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Protein

Glycine--tRNA ligase

Gene

Gars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs (By similarity).By similarity

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei203 – 2031SubstrateBy similarity
Binding sitei289 – 2891SubstrateBy similarity
Binding sitei425 – 4251SubstrateBy similarity
Binding sitei425 – 4251Substrate; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi321 – 3233ATPBy similarity
Nucleotide bindingi331 – 3366ATPBy similarity
Nucleotide bindingi447 – 4482ATPBy similarity
Nucleotide bindingi570 – 5734ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligase (EC:6.1.1.14)
Alternative name(s):
Diadenosine tetraphosphate synthetase
Short name:
AP-4-A synthetase
Glycyl-tRNA synthetase
Short name:
GlyRS
Gene namesi
Name:Gars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2449057. Gars.

Subcellular locationi

  • Cytoplasm By similarity
  • Mitochondrion By similarity
  • Cell projectionaxon By similarity

  • Note: Associated with granules in cultured neuron cells.By similarity

GO - Cellular componenti

  • axon Source: UniProtKB
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
  • nucleoplasm Source: MGI
  • secretory granule Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 729729Glycine--tRNA ligasePRO_0000072999Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei194 – 1941N6-acetyllysineBy similarity
Modified residuei443 – 4431PhosphotyrosineCombined sources
Modified residuei491 – 4911N6-acetyllysineBy similarity
Modified residuei690 – 6901PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9CZD3.
MaxQBiQ9CZD3.
PaxDbiQ9CZD3.
PRIDEiQ9CZD3.

2D gel databases

REPRODUCTION-2DPAGEQ9CZD3.

PTM databases

iPTMnetiQ9CZD3.
PhosphoSiteiQ9CZD3.
SwissPalmiQ9CZD3.

Expressioni

Gene expression databases

BgeeiQ9CZD3.
CleanExiMM_GARS.
GenevisibleiQ9CZD3. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi237263. 2 interactions.
DIPiDIP-60956N.
IntActiQ9CZD3. 2 interactions.
MINTiMINT-1856268.
STRINGi10090.ENSMUSP00000003572.

Structurei

3D structure databases

ProteinModelPortaliQ9CZD3.
SMRiQ9CZD3. Positions 108-718.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini53 – 10957WHEP-TRSPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni336 – 3405Substrate bindingBy similarity
Regioni566 – 5705Substrate bindingBy similarity

Sequence similaritiesi

Contains 1 WHEP-TRS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2298. Eukaryota.
COG0423. LUCA.
GeneTreeiENSGT00390000016949.
HOGENOMiHOG000242015.
HOVERGENiHBG036190.
InParanoidiQ9CZD3.
KOiK01880.
OMAiERFGWVE.
OrthoDBiEOG7FBRH1.
PhylomeDBiQ9CZD3.
TreeFamiTF343504.

Family and domain databases

Gene3Di1.10.287.10. 1 hit.
3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR009068. S15_NS1_RNA-bd.
IPR002315. tRNA-synt_gly.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CZD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPCLLPSLLR ATRAALPLLS PPRVVAASAS QRLLSAPAQP AASRSSMDSA
60 70 80 90 100
EELLAPLRLA VRQQGDFVRK LKEDKAPQVD VDRAVAELKA RKRVLEAKEL
110 120 130 140 150
ALQPKDDIVD RAKMEDTLKR RFFYDQAFAI YGGVSGLYDF GPVGCALKNN
160 170 180 190 200
IIQAWRQHFI QEEQILEIDC TMLTPEPVLK TSGHVDKFAD FMVKDVKNGE
210 220 230 240 250
CFRADHLLKA HLQKLMSDKK CSAEKKSEME SVLAQLDNYG QQELADLFVN
260 270 280 290 300
YNVKSPTTGN DLSPPVPFNL MFQTFIGPGG NMPGYLRPET AQGIFLNFKR
310 320 330 340 350
LLEFNQGKLP FAAAQIGNSF RNEISPRSGL IRVREFTMAE IEHFVDPTEK
360 370 380 390 400
DHPKFQSVAD LCLYLYSAKA QVTGQSARKM RLGDAVEQGV INNSVLGYFI
410 420 430 440 450
GRIYLYLTKV GISPDKLRFR QHMENEMAHY ACDCWDAESK TSYGWIEIVG
460 470 480 490 500
CADRSCYDLS CHARATKVPL VAEKPLKEPK TVNVVQFEPN KGAVGKAYKK
510 520 530 540 550
DAKLVLEYLS ACDECYISEM ELLLSEKGEF TIETEGKTFQ LTKDMVSVKR
560 570 580 590 600
FQKTLHVEEV VPSVIEPSFG LGRIMYTILE HTFHVREGDE QRTFFSFPAV
610 620 630 640 650
VAPFKCSVLP LSQNQEFMPF VKELSEALTR NGVSHKVDDS SGSIGRRYAR
660 670 680 690 700
TDEIGVAFGI TIDFDTVNKT PHTATLRDRD SMRQIRAEVS ELPNVVRDLA
710 720
NGNITWADVE ARYPLFEGQE TGKKETVEE
Length:729
Mass (Da):81,878
Last modified:June 1, 2001 - v1
Checksum:i596613F746B9C7D0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51L → V in BAE38417 (PubMed:16141072).Curated
Sequence conflicti584 – 5841H → N in BAE27003 (PubMed:16141072).Curated
Sequence conflicti597 – 5971F → L in BAE27003 (PubMed:16141072).Curated
Sequence conflicti694 – 6941N → S in AAH21747 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK146238 mRNA. Translation: BAE27003.1.
AK165857 mRNA. Translation: BAE38417.1.
BC021747 mRNA. Translation: AAH21747.1.
CCDSiCCDS39492.1.
RefSeqiNP_851009.2. NM_180678.3.
UniGeneiMm.250004.

Genome annotation databases

EnsembliENSMUST00000003572; ENSMUSP00000003572; ENSMUSG00000029777.
GeneIDi353172.
KEGGimmu:353172.
UCSCiuc009cai.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK146238 mRNA. Translation: BAE27003.1.
AK165857 mRNA. Translation: BAE38417.1.
BC021747 mRNA. Translation: AAH21747.1.
CCDSiCCDS39492.1.
RefSeqiNP_851009.2. NM_180678.3.
UniGeneiMm.250004.

3D structure databases

ProteinModelPortaliQ9CZD3.
SMRiQ9CZD3. Positions 108-718.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi237263. 2 interactions.
DIPiDIP-60956N.
IntActiQ9CZD3. 2 interactions.
MINTiMINT-1856268.
STRINGi10090.ENSMUSP00000003572.

PTM databases

iPTMnetiQ9CZD3.
PhosphoSiteiQ9CZD3.
SwissPalmiQ9CZD3.

2D gel databases

REPRODUCTION-2DPAGEQ9CZD3.

Proteomic databases

EPDiQ9CZD3.
MaxQBiQ9CZD3.
PaxDbiQ9CZD3.
PRIDEiQ9CZD3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003572; ENSMUSP00000003572; ENSMUSG00000029777.
GeneIDi353172.
KEGGimmu:353172.
UCSCiuc009cai.1. mouse.

Organism-specific databases

CTDi2617.
MGIiMGI:2449057. Gars.

Phylogenomic databases

eggNOGiKOG2298. Eukaryota.
COG0423. LUCA.
GeneTreeiENSGT00390000016949.
HOGENOMiHOG000242015.
HOVERGENiHBG036190.
InParanoidiQ9CZD3.
KOiK01880.
OMAiERFGWVE.
OrthoDBiEOG7FBRH1.
PhylomeDBiQ9CZD3.
TreeFamiTF343504.

Miscellaneous databases

ChiTaRSiGars. mouse.
PROiQ9CZD3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CZD3.
CleanExiMM_GARS.
GenevisibleiQ9CZD3. MM.

Family and domain databases

Gene3Di1.10.287.10. 1 hit.
3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR009068. S15_NS1_RNA-bd.
IPR002315. tRNA-synt_gly.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: BALB/cJ and C57BL/6J.
    Tissue: Embryo and Lung.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Salivary gland.
  3. Lubec G., Klug S.
    Submitted (MAR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 309-321; 554-573 AND 593-605, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Hippocampus.
  4. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-443, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-690, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiSYG_MOUSE
AccessioniPrimary (citable) accession number: Q9CZD3
Secondary accession number(s): Q3TMM4, Q3UK01, Q8VC67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.