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Protein

Glycine--tRNA ligase

Gene

Gars

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs (By similarity).By similarity

Catalytic activityi

ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly).By similarity
P1,P(4)-bis(5'-guanosyl) tetraphosphate + H2O = GTP + GMP.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei203SubstrateBy similarity1
Binding sitei289SubstrateBy similarity1
Binding sitei425SubstrateBy similarity1
Binding sitei425Substrate; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi321 – 323ATPBy similarity3
Nucleotide bindingi331 – 336ATPBy similarity6
Nucleotide bindingi447 – 448ATPBy similarity2
Nucleotide bindingi570 – 573ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Hydrolase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine--tRNA ligase (EC:3.6.1.17, EC:6.1.1.14)
Alternative name(s):
Diadenosine tetraphosphate synthetase
Short name:
AP-4-A synthetase
Glycyl-tRNA synthetase
Short name:
GlyRS
Gene namesi
Name:Gars
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2449057. Gars.

Subcellular locationi

  • Cytoplasm By similarity
  • Mitochondrion By similarity
  • Cell projectionaxon By similarity

  • Note: Associated with granules in cultured neuron cells.By similarity

GO - Cellular componenti

  • axon Source: UniProtKB
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
  • nucleoplasm Source: MGI
  • secretory granule Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000729991 – 729Glycine--tRNA ligaseAdd BLAST729

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei194N6-acetyllysineBy similarity1
Modified residuei443PhosphotyrosineCombined sources1
Modified residuei491N6-acetyllysineBy similarity1
Modified residuei690PhosphoserineCombined sources1
Modified residuei726PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9CZD3.
MaxQBiQ9CZD3.
PaxDbiQ9CZD3.
PeptideAtlasiQ9CZD3.
PRIDEiQ9CZD3.

2D gel databases

REPRODUCTION-2DPAGEQ9CZD3.

PTM databases

iPTMnetiQ9CZD3.
PhosphoSitePlusiQ9CZD3.
SwissPalmiQ9CZD3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029777.
CleanExiMM_GARS.
GenevisibleiQ9CZD3. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi237263. 2 interactors.
DIPiDIP-60956N.
IntActiQ9CZD3. 2 interactors.
MINTiMINT-1856268.
STRINGi10090.ENSMUSP00000003572.

Structurei

3D structure databases

ProteinModelPortaliQ9CZD3.
SMRiQ9CZD3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini53 – 109WHEP-TRSPROSITE-ProRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni336 – 340Substrate bindingBy similarity5
Regioni566 – 570Substrate bindingBy similarity5

Sequence similaritiesi

Contains 1 WHEP-TRS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2298. Eukaryota.
COG0423. LUCA.
GeneTreeiENSGT00390000016949.
HOGENOMiHOG000242015.
HOVERGENiHBG036190.
InParanoidiQ9CZD3.
KOiK01880.
OMAiERFGWVE.
OrthoDBiEOG091G02U0.
PhylomeDBiQ9CZD3.
TreeFamiTF343504.

Family and domain databases

CDDicd00774. GlyRS-like_core. 1 hit.
Gene3Di1.10.287.10. 1 hit.
3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR033731. GlyRS-like_core.
IPR009068. S15_NS1_RNA-bd.
IPR002315. tRNA-synt_gly.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CZD3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPCLLPSLLR ATRAALPLLS PPRVVAASAS QRLLSAPAQP AASRSSMDSA
60 70 80 90 100
EELLAPLRLA VRQQGDFVRK LKEDKAPQVD VDRAVAELKA RKRVLEAKEL
110 120 130 140 150
ALQPKDDIVD RAKMEDTLKR RFFYDQAFAI YGGVSGLYDF GPVGCALKNN
160 170 180 190 200
IIQAWRQHFI QEEQILEIDC TMLTPEPVLK TSGHVDKFAD FMVKDVKNGE
210 220 230 240 250
CFRADHLLKA HLQKLMSDKK CSAEKKSEME SVLAQLDNYG QQELADLFVN
260 270 280 290 300
YNVKSPTTGN DLSPPVPFNL MFQTFIGPGG NMPGYLRPET AQGIFLNFKR
310 320 330 340 350
LLEFNQGKLP FAAAQIGNSF RNEISPRSGL IRVREFTMAE IEHFVDPTEK
360 370 380 390 400
DHPKFQSVAD LCLYLYSAKA QVTGQSARKM RLGDAVEQGV INNSVLGYFI
410 420 430 440 450
GRIYLYLTKV GISPDKLRFR QHMENEMAHY ACDCWDAESK TSYGWIEIVG
460 470 480 490 500
CADRSCYDLS CHARATKVPL VAEKPLKEPK TVNVVQFEPN KGAVGKAYKK
510 520 530 540 550
DAKLVLEYLS ACDECYISEM ELLLSEKGEF TIETEGKTFQ LTKDMVSVKR
560 570 580 590 600
FQKTLHVEEV VPSVIEPSFG LGRIMYTILE HTFHVREGDE QRTFFSFPAV
610 620 630 640 650
VAPFKCSVLP LSQNQEFMPF VKELSEALTR NGVSHKVDDS SGSIGRRYAR
660 670 680 690 700
TDEIGVAFGI TIDFDTVNKT PHTATLRDRD SMRQIRAEVS ELPNVVRDLA
710 720
NGNITWADVE ARYPLFEGQE TGKKETVEE
Length:729
Mass (Da):81,878
Last modified:June 1, 2001 - v1
Checksum:i596613F746B9C7D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5L → V in BAE38417 (PubMed:16141072).Curated1
Sequence conflicti584H → N in BAE27003 (PubMed:16141072).Curated1
Sequence conflicti597F → L in BAE27003 (PubMed:16141072).Curated1
Sequence conflicti694N → S in AAH21747 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK146238 mRNA. Translation: BAE27003.1.
AK165857 mRNA. Translation: BAE38417.1.
BC021747 mRNA. Translation: AAH21747.1.
CCDSiCCDS39492.1.
RefSeqiNP_851009.2. NM_180678.3.
UniGeneiMm.250004.

Genome annotation databases

EnsembliENSMUST00000003572; ENSMUSP00000003572; ENSMUSG00000029777.
GeneIDi353172.
KEGGimmu:353172.
UCSCiuc009cai.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK146238 mRNA. Translation: BAE27003.1.
AK165857 mRNA. Translation: BAE38417.1.
BC021747 mRNA. Translation: AAH21747.1.
CCDSiCCDS39492.1.
RefSeqiNP_851009.2. NM_180678.3.
UniGeneiMm.250004.

3D structure databases

ProteinModelPortaliQ9CZD3.
SMRiQ9CZD3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi237263. 2 interactors.
DIPiDIP-60956N.
IntActiQ9CZD3. 2 interactors.
MINTiMINT-1856268.
STRINGi10090.ENSMUSP00000003572.

PTM databases

iPTMnetiQ9CZD3.
PhosphoSitePlusiQ9CZD3.
SwissPalmiQ9CZD3.

2D gel databases

REPRODUCTION-2DPAGEQ9CZD3.

Proteomic databases

EPDiQ9CZD3.
MaxQBiQ9CZD3.
PaxDbiQ9CZD3.
PeptideAtlasiQ9CZD3.
PRIDEiQ9CZD3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003572; ENSMUSP00000003572; ENSMUSG00000029777.
GeneIDi353172.
KEGGimmu:353172.
UCSCiuc009cai.1. mouse.

Organism-specific databases

CTDi2617.
MGIiMGI:2449057. Gars.

Phylogenomic databases

eggNOGiKOG2298. Eukaryota.
COG0423. LUCA.
GeneTreeiENSGT00390000016949.
HOGENOMiHOG000242015.
HOVERGENiHBG036190.
InParanoidiQ9CZD3.
KOiK01880.
OMAiERFGWVE.
OrthoDBiEOG091G02U0.
PhylomeDBiQ9CZD3.
TreeFamiTF343504.

Miscellaneous databases

ChiTaRSiGars. mouse.
PROiQ9CZD3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029777.
CleanExiMM_GARS.
GenevisibleiQ9CZD3. MM.

Family and domain databases

CDDicd00774. GlyRS-like_core. 1 hit.
Gene3Di1.10.287.10. 1 hit.
3.40.50.800. 1 hit.
InterProiIPR002314. aa-tRNA-synt_IIb.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR027031. Gly-tRNA_synthase/POLG2.
IPR033731. GlyRS-like_core.
IPR009068. S15_NS1_RNA-bd.
IPR002315. tRNA-synt_gly.
IPR000738. WHEP-TRS_dom.
[Graphical view]
PANTHERiPTHR10745. PTHR10745. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF00458. WHEP-TRS. 1 hit.
[Graphical view]
PRINTSiPR01043. TRNASYNTHGLY.
SMARTiSM00991. WHEP-TRS. 1 hit.
[Graphical view]
SUPFAMiSSF47060. SSF47060. 1 hit.
SSF52954. SSF52954. 1 hit.
TIGRFAMsiTIGR00389. glyS_dimeric. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
PS00762. WHEP_TRS_1. 1 hit.
PS51185. WHEP_TRS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYG_MOUSE
AccessioniPrimary (citable) accession number: Q9CZD3
Secondary accession number(s): Q3TMM4, Q3UK01, Q8VC67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.