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Protein

Mediator of RNA polymerase II transcription subunit 7

Gene

Med7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • regulation of transcription by RNA polymerase II Source: MGI
  • stem cell population maintenance Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway
R-MMU-442533 Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 7
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 9
Short name:
CRSP complex subunit 9
Mediator complex subunit 7
Gene namesi
Name:Med7
Synonyms:Crsp9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1913463 Med7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000794091 – 233Mediator of RNA polymerase II transcription subunit 7Add BLAST233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki185Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki185Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei195PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9CZB6
MaxQBiQ9CZB6
PaxDbiQ9CZB6
PeptideAtlasiQ9CZB6
PRIDEiQ9CZB6

PTM databases

iPTMnetiQ9CZB6
PhosphoSitePlusiQ9CZB6

Expressioni

Gene expression databases

BgeeiENSMUSG00000020397
CleanExiMM_MED7
ExpressionAtlasiQ9CZB6 baseline and differential
GenevisibleiQ9CZB6 MM

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Protein-protein interaction databases

BioGridi211303, 2 interactors
CORUMiQ9CZB6
IntActiQ9CZB6, 2 interactors
STRINGi10090.ENSMUSP00000020665

Structurei

3D structure databases

ProteinModelPortaliQ9CZB6
SMRiQ9CZB6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 14Poly-Pro4
Compositional biasi32 – 37Poly-Pro6

Sequence similaritiesi

Belongs to the Mediator complex subunit 7 family.Curated

Phylogenomic databases

eggNOGiKOG0570 Eukaryota
ENOG410ZFRU LUCA
GeneTreeiENSGT00530000064187
HOGENOMiHOG000241102
HOVERGENiHBG002997
InParanoidiQ9CZB6
KOiK15148
OMAiKRLYPQH
OrthoDBiEOG091G0N9D
PhylomeDBiQ9CZB6
TreeFamiTF314411

Family and domain databases

InterProiView protein in InterPro
IPR037212 Med7/Med21-like
IPR009244 Mediatior_Med7
PANTHERiPTHR21428 PTHR21428, 1 hit
PfamiView protein in Pfam
PF05983 Med7, 1 hit
SUPFAMiSSF140718 SSF140718, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CZB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ
60 70 80 90 100
CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINFL DLLDILIRSP
110 120 130 140 150
GSIKREEKLE DLKLLFVHVH HLINEYRPHQ ARETLRVMME VQKRQRLETA
160 170 180 190 200
ERFQKHLERV IEMIQNCLAS LPDDLPHSEA GMRVKAEPMD TDDNSNCPGQ
210 220 230
NEQQRESSGH RRDQIIEKDA ALCVLIDEMN ERP
Length:233
Mass (Da):27,205
Last modified:April 12, 2005 - v2
Checksum:iCB340CCF5770541C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26I → S in AAH11333 (PubMed:15489334).Curated1
Sequence conflicti33 – 34KP → GR in AAH11333 (PubMed:15489334).Curated2
Sequence conflicti49 – 50FQ → KR in AAH11333 (PubMed:15489334).Curated2
Sequence conflicti73D → Y in BAB23272 (PubMed:16141072).Curated1
Sequence conflicti88N → T in BAB23272 (PubMed:16141072).Curated1
Sequence conflicti91D → N in BAB28478 (PubMed:16141072).Curated1
Sequence conflicti104K → N in BAB28478 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004354 mRNA Translation: BAB23272.1
AK010357 mRNA Translation: BAB26877.1
AK012799 mRNA Translation: BAB28478.1
AK144918 mRNA Translation: BAE26135.1
AK147739 mRNA Translation: BAE28106.1
AK167407 mRNA Translation: BAE39496.1
AL669948 Genomic DNA No translation available.
BC011333 mRNA Translation: AAH11333.1
CCDSiCCDS36134.1
RefSeqiNP_001098000.1, NM_001104530.1
NP_001098026.1, NM_001104556.1
NP_001098027.1, NM_001104557.1
NP_079702.3, NM_025426.3
XP_006533994.1, XM_006533931.3
XP_006533995.1, XM_006533932.3
XP_006533996.1, XM_006533933.3
XP_006533997.1, XM_006533934.3
UniGeneiMm.24159

Genome annotation databases

EnsembliENSMUST00000020665; ENSMUSP00000020665; ENSMUSG00000020397
ENSMUST00000109232; ENSMUSP00000104855; ENSMUSG00000020397
ENSMUST00000170928; ENSMUSP00000131852; ENSMUSG00000020397
GeneIDi66213
KEGGimmu:66213
UCSCiuc007iok.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMED7_MOUSE
AccessioniPrimary (citable) accession number: Q9CZB6
Secondary accession number(s): Q3UGV1
, Q91VN3, Q9CWV8, Q9D0V9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: March 28, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health