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Protein

Mediator of RNA polymerase II transcription subunit 7

Gene

Med7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

  1. RNA polymerase II transcription cofactor activity Source: InterPro

GO - Biological processi

  1. regulation of transcription from RNA polymerase II promoter Source: MGI
  2. stem cell maintenance Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.
REACT_275829. Generic Transcription Pathway.
REACT_309805. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 7
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 9
Short name:
CRSP complex subunit 9
Mediator complex subunit 7
Gene namesi
Name:Med7
Synonyms:Crsp9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1913463. Med7.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. mediator complex Source: MGI
  2. mitochondrion Source: MGI
  3. nuclear membrane Source: MGI
  4. nucleoplasm Source: MGI
  5. nucleus Source: MGI
  6. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 233233Mediator of RNA polymerase II transcription subunit 7PRO_0000079409Add
BLAST

Proteomic databases

MaxQBiQ9CZB6.
PaxDbiQ9CZB6.
PRIDEiQ9CZB6.

PTM databases

PhosphoSiteiQ9CZB6.

Expressioni

Gene expression databases

BgeeiQ9CZB6.
CleanExiMM_MED7.
ExpressionAtlasiQ9CZB6. baseline and differential.
GenevestigatoriQ9CZB6.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Protein-protein interaction databases

BioGridi211303. 2 interactions.
IntActiQ9CZB6. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9CZB6.
SMRiQ9CZB6. Positions 106-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi11 – 144Poly-Pro
Compositional biasi32 – 376Poly-Pro

Sequence similaritiesi

Belongs to the Mediator complex subunit 7 family.Curated

Phylogenomic databases

eggNOGiNOG274591.
GeneTreeiENSGT00530000064187.
HOGENOMiHOG000241102.
HOVERGENiHBG002997.
InParanoidiQ9CZB6.
KOiK15148.
OMAiMCSIIDE.
PhylomeDBiQ9CZB6.
TreeFamiTF314411.

Family and domain databases

InterProiIPR009244. Mediatior_Med7.
[Graphical view]
PfamiPF05983. Med7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CZB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ
60 70 80 90 100
CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINFL DLLDILIRSP
110 120 130 140 150
GSIKREEKLE DLKLLFVHVH HLINEYRPHQ ARETLRVMME VQKRQRLETA
160 170 180 190 200
ERFQKHLERV IEMIQNCLAS LPDDLPHSEA GMRVKAEPMD TDDNSNCPGQ
210 220 230
NEQQRESSGH RRDQIIEKDA ALCVLIDEMN ERP
Length:233
Mass (Da):27,205
Last modified:April 11, 2005 - v2
Checksum:iCB340CCF5770541C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261I → S in AAH11333 (PubMed:15489334).Curated
Sequence conflicti33 – 342KP → GR in AAH11333 (PubMed:15489334).Curated
Sequence conflicti49 – 502FQ → KR in AAH11333 (PubMed:15489334).Curated
Sequence conflicti73 – 731D → Y in BAB23272 (PubMed:16141072).Curated
Sequence conflicti88 – 881N → T in BAB23272 (PubMed:16141072).Curated
Sequence conflicti91 – 911D → N in BAB28478 (PubMed:16141072).Curated
Sequence conflicti104 – 1041K → N in BAB28478 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004354 mRNA. Translation: BAB23272.1.
AK010357 mRNA. Translation: BAB26877.1.
AK012799 mRNA. Translation: BAB28478.1.
AK144918 mRNA. Translation: BAE26135.1.
AK147739 mRNA. Translation: BAE28106.1.
AK167407 mRNA. Translation: BAE39496.1.
AL669948 Genomic DNA. No translation available.
BC011333 mRNA. Translation: AAH11333.1.
CCDSiCCDS36134.1.
RefSeqiNP_001098000.1. NM_001104530.1.
NP_001098026.1. NM_001104556.1.
NP_001098027.1. NM_001104557.1.
NP_079702.3. NM_025426.3.
XP_006533994.1. XM_006533931.2.
XP_006533995.1. XM_006533932.2.
XP_006533996.1. XM_006533933.2.
XP_006533997.1. XM_006533934.2.
UniGeneiMm.24159.

Genome annotation databases

EnsembliENSMUST00000020665; ENSMUSP00000020665; ENSMUSG00000020397.
ENSMUST00000109232; ENSMUSP00000104855; ENSMUSG00000020397.
ENSMUST00000170928; ENSMUSP00000131852; ENSMUSG00000020397.
GeneIDi66213.
KEGGimmu:66213.
UCSCiuc007iok.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004354 mRNA. Translation: BAB23272.1.
AK010357 mRNA. Translation: BAB26877.1.
AK012799 mRNA. Translation: BAB28478.1.
AK144918 mRNA. Translation: BAE26135.1.
AK147739 mRNA. Translation: BAE28106.1.
AK167407 mRNA. Translation: BAE39496.1.
AL669948 Genomic DNA. No translation available.
BC011333 mRNA. Translation: AAH11333.1.
CCDSiCCDS36134.1.
RefSeqiNP_001098000.1. NM_001104530.1.
NP_001098026.1. NM_001104556.1.
NP_001098027.1. NM_001104557.1.
NP_079702.3. NM_025426.3.
XP_006533994.1. XM_006533931.2.
XP_006533995.1. XM_006533932.2.
XP_006533996.1. XM_006533933.2.
XP_006533997.1. XM_006533934.2.
UniGeneiMm.24159.

3D structure databases

ProteinModelPortaliQ9CZB6.
SMRiQ9CZB6. Positions 106-170.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211303. 2 interactions.
IntActiQ9CZB6. 2 interactions.

PTM databases

PhosphoSiteiQ9CZB6.

Proteomic databases

MaxQBiQ9CZB6.
PaxDbiQ9CZB6.
PRIDEiQ9CZB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020665; ENSMUSP00000020665; ENSMUSG00000020397.
ENSMUST00000109232; ENSMUSP00000104855; ENSMUSG00000020397.
ENSMUST00000170928; ENSMUSP00000131852; ENSMUSG00000020397.
GeneIDi66213.
KEGGimmu:66213.
UCSCiuc007iok.2. mouse.

Organism-specific databases

CTDi9443.
MGIiMGI:1913463. Med7.

Phylogenomic databases

eggNOGiNOG274591.
GeneTreeiENSGT00530000064187.
HOGENOMiHOG000241102.
HOVERGENiHBG002997.
InParanoidiQ9CZB6.
KOiK15148.
OMAiMCSIIDE.
PhylomeDBiQ9CZB6.
TreeFamiTF314411.

Enzyme and pathway databases

ReactomeiREACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.
REACT_275829. Generic Transcription Pathway.
REACT_309805. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

ChiTaRSiMed7. mouse.
NextBioi320983.
PROiQ9CZB6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CZB6.
CleanExiMM_MED7.
ExpressionAtlasiQ9CZB6. baseline and differential.
GenevestigatoriQ9CZB6.

Family and domain databases

InterProiIPR009244. Mediatior_Med7.
[Graphical view]
PfamiPF05983. Med7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo, Embryonic stem cell, Mammary gland, Melanocyte and Placenta.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.

Entry informationi

Entry nameiMED7_MOUSE
AccessioniPrimary (citable) accession number: Q9CZB6
Secondary accession number(s): Q3UGV1
, Q91VN3, Q9CWV8, Q9D0V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 11, 2005
Last sequence update: April 11, 2005
Last modified: March 31, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.