Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mediator of RNA polymerase II transcription subunit 7

Gene

Med7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 7
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 9
Short name:
CRSP complex subunit 9
Mediator complex subunit 7
Gene namesi
Name:Med7
Synonyms:Crsp9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1913463. Med7.

Subcellular locationi

GO - Cellular componenti

  • mediator complex Source: MGI
  • nucleoplasm Source: Reactome
  • transcription factor complex Source: MGI
  • ubiquitin ligase complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000794091 – 233Mediator of RNA polymerase II transcription subunit 7Add BLAST233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki185Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki185Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei195PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9CZB6.
MaxQBiQ9CZB6.
PaxDbiQ9CZB6.
PeptideAtlasiQ9CZB6.
PRIDEiQ9CZB6.

PTM databases

iPTMnetiQ9CZB6.
PhosphoSitePlusiQ9CZB6.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020397.
CleanExiMM_MED7.
ExpressionAtlasiQ9CZB6. baseline and differential.
GenevisibleiQ9CZB6. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Protein-protein interaction databases

BioGridi211303. 2 interactors.
IntActiQ9CZB6. 2 interactors.
STRINGi10090.ENSMUSP00000020665.

Structurei

3D structure databases

ProteinModelPortaliQ9CZB6.
SMRiQ9CZB6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi11 – 14Poly-Pro4
Compositional biasi32 – 37Poly-Pro6

Sequence similaritiesi

Belongs to the Mediator complex subunit 7 family.Curated

Phylogenomic databases

eggNOGiKOG0570. Eukaryota.
ENOG410ZFRU. LUCA.
GeneTreeiENSGT00530000064187.
HOGENOMiHOG000241102.
HOVERGENiHBG002997.
InParanoidiQ9CZB6.
KOiK15148.
OMAiLRVMMEL.
OrthoDBiEOG091G0N9D.
PhylomeDBiQ9CZB6.
TreeFamiTF314411.

Family and domain databases

InterProiIPR009244. Mediatior_Med7.
[Graphical view]
PfamiPF05983. Med7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CZB6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEPQQVSAL PPPPMQYIKE YTDENIQEGL APKPPPPIKD SYMMFGNQFQ
60 70 80 90 100
CDDLIIRPLE SQGIERLHPM QFDHKKELRK LNMSILINFL DLLDILIRSP
110 120 130 140 150
GSIKREEKLE DLKLLFVHVH HLINEYRPHQ ARETLRVMME VQKRQRLETA
160 170 180 190 200
ERFQKHLERV IEMIQNCLAS LPDDLPHSEA GMRVKAEPMD TDDNSNCPGQ
210 220 230
NEQQRESSGH RRDQIIEKDA ALCVLIDEMN ERP
Length:233
Mass (Da):27,205
Last modified:April 12, 2005 - v2
Checksum:iCB340CCF5770541C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26I → S in AAH11333 (PubMed:15489334).Curated1
Sequence conflicti33 – 34KP → GR in AAH11333 (PubMed:15489334).Curated2
Sequence conflicti49 – 50FQ → KR in AAH11333 (PubMed:15489334).Curated2
Sequence conflicti73D → Y in BAB23272 (PubMed:16141072).Curated1
Sequence conflicti88N → T in BAB23272 (PubMed:16141072).Curated1
Sequence conflicti91D → N in BAB28478 (PubMed:16141072).Curated1
Sequence conflicti104K → N in BAB28478 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004354 mRNA. Translation: BAB23272.1.
AK010357 mRNA. Translation: BAB26877.1.
AK012799 mRNA. Translation: BAB28478.1.
AK144918 mRNA. Translation: BAE26135.1.
AK147739 mRNA. Translation: BAE28106.1.
AK167407 mRNA. Translation: BAE39496.1.
AL669948 Genomic DNA. No translation available.
BC011333 mRNA. Translation: AAH11333.1.
CCDSiCCDS36134.1.
RefSeqiNP_001098000.1. NM_001104530.1.
NP_001098026.1. NM_001104556.1.
NP_001098027.1. NM_001104557.1.
NP_079702.3. NM_025426.3.
XP_006533994.1. XM_006533931.3.
XP_006533995.1. XM_006533932.3.
XP_006533996.1. XM_006533933.3.
XP_006533997.1. XM_006533934.3.
UniGeneiMm.24159.

Genome annotation databases

EnsembliENSMUST00000020665; ENSMUSP00000020665; ENSMUSG00000020397.
ENSMUST00000109232; ENSMUSP00000104855; ENSMUSG00000020397.
ENSMUST00000170928; ENSMUSP00000131852; ENSMUSG00000020397.
GeneIDi66213.
KEGGimmu:66213.
UCSCiuc007iok.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004354 mRNA. Translation: BAB23272.1.
AK010357 mRNA. Translation: BAB26877.1.
AK012799 mRNA. Translation: BAB28478.1.
AK144918 mRNA. Translation: BAE26135.1.
AK147739 mRNA. Translation: BAE28106.1.
AK167407 mRNA. Translation: BAE39496.1.
AL669948 Genomic DNA. No translation available.
BC011333 mRNA. Translation: AAH11333.1.
CCDSiCCDS36134.1.
RefSeqiNP_001098000.1. NM_001104530.1.
NP_001098026.1. NM_001104556.1.
NP_001098027.1. NM_001104557.1.
NP_079702.3. NM_025426.3.
XP_006533994.1. XM_006533931.3.
XP_006533995.1. XM_006533932.3.
XP_006533996.1. XM_006533933.3.
XP_006533997.1. XM_006533934.3.
UniGeneiMm.24159.

3D structure databases

ProteinModelPortaliQ9CZB6.
SMRiQ9CZB6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211303. 2 interactors.
IntActiQ9CZB6. 2 interactors.
STRINGi10090.ENSMUSP00000020665.

PTM databases

iPTMnetiQ9CZB6.
PhosphoSitePlusiQ9CZB6.

Proteomic databases

EPDiQ9CZB6.
MaxQBiQ9CZB6.
PaxDbiQ9CZB6.
PeptideAtlasiQ9CZB6.
PRIDEiQ9CZB6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020665; ENSMUSP00000020665; ENSMUSG00000020397.
ENSMUST00000109232; ENSMUSP00000104855; ENSMUSG00000020397.
ENSMUST00000170928; ENSMUSP00000131852; ENSMUSG00000020397.
GeneIDi66213.
KEGGimmu:66213.
UCSCiuc007iok.2. mouse.

Organism-specific databases

CTDi9443.
MGIiMGI:1913463. Med7.

Phylogenomic databases

eggNOGiKOG0570. Eukaryota.
ENOG410ZFRU. LUCA.
GeneTreeiENSGT00530000064187.
HOGENOMiHOG000241102.
HOVERGENiHBG002997.
InParanoidiQ9CZB6.
KOiK15148.
OMAiLRVMMEL.
OrthoDBiEOG091G0N9D.
PhylomeDBiQ9CZB6.
TreeFamiTF314411.

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSiMed7. mouse.
PROiQ9CZB6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020397.
CleanExiMM_MED7.
ExpressionAtlasiQ9CZB6. baseline and differential.
GenevisibleiQ9CZB6. MM.

Family and domain databases

InterProiIPR009244. Mediatior_Med7.
[Graphical view]
PfamiPF05983. Med7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMED7_MOUSE
AccessioniPrimary (citable) accession number: Q9CZB6
Secondary accession number(s): Q3UGV1
, Q91VN3, Q9CWV8, Q9D0V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: April 12, 2005
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.