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Protein

Nuclear distribution protein nudE homolog 1

Gene

Nde1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.1 Publication

GO - Molecular functioni

  • identical protein binding Source: MGI
  • microtubule binding Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centrosome duplication Source: UniProtKB
  • centrosome localization Source: GO_Central
  • cerebral cortex development Source: MGI
  • chromosome segregation Source: GO_Central
  • establishment of chromosome localization Source: MGI
  • establishment of mitotic spindle orientation Source: MGI
  • forebrain development Source: MGI
  • microtubule nucleation Source: MGI
  • microtubule organizing center organization Source: UniProtKB
  • mitotic centrosome separation Source: GO_Central
  • neuroblast proliferation Source: MGI
  • neuron migration Source: UniProtKB
  • vesicle transport along microtubule Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Cell cycle, Cell division, Differentiation, Mitosis, Neurogenesis

Enzyme and pathway databases

ReactomeiR-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.
R-MMU-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear distribution protein nudE homolog 1
Short name:
NudE
Short name:
mNudE
Gene namesi
Name:Nde1
Synonyms:Nude
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1914453. Nde1.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: MGI
  • cleavage furrow Source: UniProtKB-SubCell
  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytoplasm Source: UniProtKB-KW
  • kinesin complex Source: GO_Central
  • kinetochore Source: MGI
  • membrane Source: MGI
  • microtubule Source: UniProtKB-KW
  • microtubule organizing center Source: UniProtKB
  • spindle Source: MGI
  • spindle pole centrosome Source: UniProtKB
  • synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Nuclear distribution protein nudE homolog 1PRO_0000240203Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei211 – 2111PhosphoserineBy similarity
Modified residuei215 – 2151PhosphothreonineBy similarity
Modified residuei228 – 2281PhosphothreonineBy similarity
Modified residuei239 – 2391PhosphoserineBy similarity
Modified residuei243 – 2431PhosphothreonineBy similarity
Modified residuei246 – 2461Phosphothreonine1 Publication
Lipidationi274 – 2741S-palmitoyl cysteine; by ZDHHC2, ZDHHC3 and ZDHHC7By similarity
Modified residuei282 – 2821PhosphoserineBy similarity
Modified residuei309 – 3091PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated in mitosis (By similarity). Phosphorylation at Thr-246 is essential for the G2/M transition.By similarity1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ9CZA6.
PaxDbiQ9CZA6.
PeptideAtlasiQ9CZA6.
PRIDEiQ9CZA6.

PTM databases

iPTMnetiQ9CZA6.
PhosphoSiteiQ9CZA6.
SwissPalmiQ9CZA6.

Expressioni

Tissue specificityi

Highly expressed in ovary. Also expressed in brain, heart, kidney, large intestine, liver, lung, small intestine and testis.2 Publications

Developmental stagei

Widely expressed in the developing brain, particularly in the neural progenitor cells of ventricular zone and the intermediate zone of the cerebral cortex. Expression peaks at E11 and declines at E15 and E17. After completion of neuronal migration expression is reduced in the cortex.2 Publications

Gene expression databases

BgeeiENSMUSG00000022678.
ExpressionAtlasiQ9CZA6. baseline and differential.
GenevisibleiQ9CZA6. MM.

Interactioni

Subunit structurei

Interacts with dynactin and PCM1 (By similarity). Self-associates. Interacts with CENPF, LIS1, CNTRL, dynein, tubulin gamma, PAFAH1B1, PCNT, SLMAP and TCP1. Interacts with ZNF365 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
CenpfQ155P78EBI-309934,EBI-2211248
DYNC1I1O145762EBI-309934,EBI-366267From a different organism.
PAFAH1B1P430346EBI-309934,EBI-720620From a different organism.

GO - Molecular functioni

  • identical protein binding Source: MGI
  • microtubule binding Source: UniProtKB

Protein-protein interaction databases

BioGridi212013. 11 interactions.
DIPiDIP-54638N.
IntActiQ9CZA6. 17 interactions.
MINTiMINT-219016.
STRINGi10090.ENSMUSP00000023359.

Structurei

3D structure databases

ProteinModelPortaliQ9CZA6.
SMRiQ9CZA6. Positions 8-167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9393Self-associationAdd
BLAST
Regioni88 – 15669Interaction with PAFAH1B1Add
BLAST
Regioni167 – 290124Interaction with CENPFAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili18 – 188171Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the nudE family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1853. Eukaryota.
ENOG410XPMP. LUCA.
GeneTreeiENSGT00390000000111.
HOGENOMiHOG000280681.
HOVERGENiHBG082010.
InParanoidiQ9CZA6.
KOiK16738.
OMAiCERPATL.
OrthoDBiEOG091G0GBV.
PhylomeDBiQ9CZA6.
TreeFamiTF325693.

Family and domain databases

InterProiIPR033494. NUDE.
IPR006964. NUDE_dom.
[Graphical view]
PANTHERiPTHR10921. PTHR10921. 1 hit.
PfamiPF04880. NUDE_C. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CZA6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDSGKTFES EEEETNYWRD LAMTYKQRAE NTQEELREFQ EGSREYEAEL
60 70 80 90 100
EAQLQQIETR NRDLLSENNR LRMELESVKE KFEMQHSEGY RQISALEDDL
110 120 130 140 150
AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA
160 170 180 190 200
FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQDKPR TPMPGSGQAK
210 220 230 240 250
RTDMAVQATG SVPSTPVAHR GPSSGLNTPG MFRRGLDSST SGTPLTPAAR
260 270 280 290 300
ISALNIVGDL LRKVGALESK LASCRNFMYD QSPSRTSGPA SGRGTKNRDG
310 320 330 340
VDRRPGSTSV GDKGSGKRLE FGKPASEPAS PALPSAQGVV KLLL
Length:344
Mass (Da):38,523
Last modified:June 1, 2001 - v1
Checksum:iC0A339C4F51EF8D9
GO
Isoform 2 (identifier: Q9CZA6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     315-317: SGK → REN
     318-344: Missing.

Note: No experimental confirmation available.
Show »
Length:317
Mass (Da):35,892
Checksum:i714BD7DD448D4EB3
GO
Isoform 3 (identifier: Q9CZA6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-317: GK → LP
     318-344: Missing.

Note: No experimental confirmation available.
Show »
Length:317
Mass (Da):35,790
Checksum:i6267E66D448D4EB3
GO
Isoform 4 (identifier: Q9CZA6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-132: Missing.

Show »
Length:212
Mass (Da):22,682
Checksum:iD9D5D1046E384F2E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 132132Missing in isoform 4. 1 PublicationVSP_019306Add
BLAST
Alternative sequencei315 – 3173SGK → REN in isoform 2. 1 PublicationVSP_019307
Alternative sequencei316 – 3172GK → LP in isoform 3. 1 PublicationVSP_019308
Alternative sequencei318 – 34427Missing in isoform 2 and isoform 3. 1 PublicationVSP_019309Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322073 mRNA. Translation: AAK08067.1.
AF290473 mRNA. Translation: AAG10062.1.
AK012830 mRNA. Translation: BAB28499.1.
AK037289 mRNA. Translation: BAE20509.1.
AK146982 mRNA. Translation: BAE27585.1.
AK150826 mRNA. Translation: BAE29888.1.
AK169743 mRNA. Translation: BAE41342.1.
BC023267 mRNA. Translation: AAH23267.1.
CCDSiCCDS37263.2. [Q9CZA6-1]
CCDS49770.1. [Q9CZA6-3]
CCDS70688.1. [Q9CZA6-2]
RefSeqiNP_001107557.1. NM_001114085.1. [Q9CZA6-3]
NP_001272432.1. NM_001285503.1. [Q9CZA6-2]
NP_001272433.1. NM_001285504.1. [Q9CZA6-4]
NP_075806.2. NM_023317.2. [Q9CZA6-1]
XP_011244302.1. XM_011246000.1. [Q9CZA6-4]
UniGeneiMm.24105.

Genome annotation databases

EnsembliENSMUST00000023359; ENSMUSP00000023359; ENSMUSG00000022678. [Q9CZA6-1]
ENSMUST00000115795; ENSMUSP00000111461; ENSMUSG00000022678. [Q9CZA6-2]
ENSMUST00000117958; ENSMUSP00000112817; ENSMUSG00000022678. [Q9CZA6-3]
GeneIDi67203.
KEGGimmu:67203.
UCSCiuc007ygy.2. mouse. [Q9CZA6-1]
uc007yha.2. mouse. [Q9CZA6-3]
uc007yhb.3. mouse. [Q9CZA6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322073 mRNA. Translation: AAK08067.1.
AF290473 mRNA. Translation: AAG10062.1.
AK012830 mRNA. Translation: BAB28499.1.
AK037289 mRNA. Translation: BAE20509.1.
AK146982 mRNA. Translation: BAE27585.1.
AK150826 mRNA. Translation: BAE29888.1.
AK169743 mRNA. Translation: BAE41342.1.
BC023267 mRNA. Translation: AAH23267.1.
CCDSiCCDS37263.2. [Q9CZA6-1]
CCDS49770.1. [Q9CZA6-3]
CCDS70688.1. [Q9CZA6-2]
RefSeqiNP_001107557.1. NM_001114085.1. [Q9CZA6-3]
NP_001272432.1. NM_001285503.1. [Q9CZA6-2]
NP_001272433.1. NM_001285504.1. [Q9CZA6-4]
NP_075806.2. NM_023317.2. [Q9CZA6-1]
XP_011244302.1. XM_011246000.1. [Q9CZA6-4]
UniGeneiMm.24105.

3D structure databases

ProteinModelPortaliQ9CZA6.
SMRiQ9CZA6. Positions 8-167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212013. 11 interactions.
DIPiDIP-54638N.
IntActiQ9CZA6. 17 interactions.
MINTiMINT-219016.
STRINGi10090.ENSMUSP00000023359.

PTM databases

iPTMnetiQ9CZA6.
PhosphoSiteiQ9CZA6.
SwissPalmiQ9CZA6.

Proteomic databases

MaxQBiQ9CZA6.
PaxDbiQ9CZA6.
PeptideAtlasiQ9CZA6.
PRIDEiQ9CZA6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023359; ENSMUSP00000023359; ENSMUSG00000022678. [Q9CZA6-1]
ENSMUST00000115795; ENSMUSP00000111461; ENSMUSG00000022678. [Q9CZA6-2]
ENSMUST00000117958; ENSMUSP00000112817; ENSMUSG00000022678. [Q9CZA6-3]
GeneIDi67203.
KEGGimmu:67203.
UCSCiuc007ygy.2. mouse. [Q9CZA6-1]
uc007yha.2. mouse. [Q9CZA6-3]
uc007yhb.3. mouse. [Q9CZA6-2]

Organism-specific databases

CTDi54820.
MGIiMGI:1914453. Nde1.

Phylogenomic databases

eggNOGiKOG1853. Eukaryota.
ENOG410XPMP. LUCA.
GeneTreeiENSGT00390000000111.
HOGENOMiHOG000280681.
HOVERGENiHBG082010.
InParanoidiQ9CZA6.
KOiK16738.
OMAiCERPATL.
OrthoDBiEOG091G0GBV.
PhylomeDBiQ9CZA6.
TreeFamiTF325693.

Enzyme and pathway databases

ReactomeiR-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.
R-MMU-8854518. AURKA Activation by TPX2.

Miscellaneous databases

PROiQ9CZA6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022678.
ExpressionAtlasiQ9CZA6. baseline and differential.
GenevisibleiQ9CZA6. MM.

Family and domain databases

InterProiIPR033494. NUDE.
IPR006964. NUDE_dom.
[Graphical view]
PANTHERiPTHR10921. PTHR10921. 1 hit.
PfamiPF04880. NUDE_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDE1_MOUSE
AccessioniPrimary (citable) accession number: Q9CZA6
Secondary accession number(s): Q3UBS6, Q3UIC1, Q9ERR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Homozygous loss of this protein results in microcephaly that preferentially affects the cerebral cortex. Affected animals have a smaller cortex with reduced superficial cortical layers, although cortical lamination is mostly preserved. The smaller cortex size seems to reflect both reduced progenitor cell division and altered specification of cell fates following progenitor cell division.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.