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Protein

ATP-dependent (S)-NAD(P)H-hydrate dehydratase

Gene

Naxd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.UniRule annotation1 Publication

Catalytic activityi

ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH.UniRule annotation
ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate = ADP + phosphate + NADPH.

Cofactori

Mg2+UniRule annotation

Kineticsi

  1. KM=2.8 µM for (S)-NADHX1 Publication
  2. KM=2.5 µM for (S)-NADPHX1 Publication
  3. KM=1.8 µM for ATP1 Publication
  1. Vmax=0.43 µmol/min/mg enzyme toward (S)-NADHX1 Publication
  2. Vmax=1.6 µmol/min/mg enzyme toward (S)-NADPHX1 Publication
  3. Vmax=0.26 µmol/min/mg enzyme toward ATP1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei149NAD(P)HX; via amide nitrogenUniRule annotation1
Binding sitei271NAD(P)HXUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi242 – 246ATPUniRule annotation5
Nucleotide bindingi261 – 270ATPUniRule annotation10

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent (S)-NAD(P)H-hydrate dehydrataseUniRule annotation (EC:4.2.1.93UniRule annotation)
Alternative name(s):
ATP-dependent NAD(P)HX dehydrataseUniRule annotation
Carbohydrate kinase domain-containing proteinUniRule annotation
NAD(P)HX dehydrataseBy similarity
Gene namesi
Name:NaxdBy similarity
Synonyms:Carkd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1913353. Carkd.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: MGI
  • endoplasmic reticulum Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 42MitochondrionUniRule annotationAdd BLAST42
ChainiPRO_000033702243 – 343ATP-dependent (S)-NAD(P)H-hydrate dehydrataseAdd BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei63N6-acetyllysineCombined sources1
Modified residuei81PhosphotyrosineCombined sources1
Modified residuei216PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9CZ42.
PeptideAtlasiQ9CZ42.
PRIDEiQ9CZ42.

PTM databases

iPTMnetiQ9CZ42.
PhosphoSitePlusiQ9CZ42.
SwissPalmiQ9CZ42.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031505.

Interactioni

Protein-protein interaction databases

IntActiQ9CZ42. 3 interactors.
MINTiMINT-4126105.
STRINGi10090.ENSMUSP00000033901.

Structurei

3D structure databases

ProteinModelPortaliQ9CZ42.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 340YjeF C-terminalUniRule annotationAdd BLAST292

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni202 – 208NAD(P)HXUniRule annotation7

Sequence similaritiesi

Belongs to the NnrD/CARKD family.UniRule annotation
Contains 1 YjeF C-terminal domain.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG3974. Eukaryota.
COG0063. LUCA.
HOGENOMiHOG000163126.
HOVERGENiHBG057182.
InParanoidiQ9CZ42.
KOiK17757.
PhylomeDBiQ9CZ42.

Family and domain databases

CDDicd01171. YXKO-related. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01965. NADHX_dehydratase. 1 hit.
InterProiIPR000631. CARKD.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF01256. Carb_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00196. yjeF_cterm. 1 hit.
PROSITEiPS51383. YJEF_C_3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CZ42-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVHACGAAA AVVALLSAAI ALQWSPLYAV LQRALSLHTA HATKDMENLF
60 70 80 90 100
QLVRNIVPAL TSKKHKGQDG RIGIVGGCQE YTGAPYFAGI SALKVGADLT
110 120 130 140 150
HVFCAREAAP VIKSYSPELI VHPVLDSSNA VEEVEKWLPR LHALVVGPGL
160 170 180 190 200
GRDDLLLNNV RGILESTKAR DIPVVIDADG LWLVAQQPAL IHSYHKAILT
210 220 230 240 250
PNHVEFSRLW EAVLSSPMDS NDLKGSTLKL SQALGNITVV QKGEQDLISN
260 270 280 290 300
GQQVLVCNQE GSSRRCGGQG DLLSGSLGVM VHWALRAGPE KTNGSSPLLV
310 320 330 340
AAWGACTLTR ECNRQAFQKY GRSTTTTDMI TEVGTAFSRL FTT
Length:343
Mass (Da):36,717
Last modified:June 1, 2001 - v1
Checksum:iD0202FE6716F80D5
GO
Isoform 2 (identifier: Q9CZ42-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-45: Missing.

Show »
Length:298
Mass (Da):32,152
Checksum:i45454F0628FFD33D
GO
Isoform 3 (identifier: Q9CZ42-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-29: MAVHACGAAAAVVALLSAAIALQWSPLYA → MGFRCVAIRACGG

Show »
Length:327
Mass (Da):35,217
Checksum:i871CFDE6941678A4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti203H → N in BAB22531 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0338311 – 45Missing in isoform 2. 2 PublicationsAdd BLAST45
Alternative sequenceiVSP_0338321 – 29MAVHA…SPLYA → MGFRCVAIRACGG in isoform 3. 1 PublicationAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003048 mRNA. Translation: BAB22531.1.
AK009254 mRNA. Translation: BAB26172.1.
AK012456 mRNA. Translation: BAB28251.1.
AK013028 mRNA. Translation: BAB28607.1.
AK013069 mRNA. Translation: BAB28632.1.
AK013420 mRNA. Translation: BAB28847.1.
AK154142 mRNA. Translation: BAE32403.1.
AK171034 mRNA. Translation: BAE42200.1.
BC019538 mRNA. Translation: AAH19538.1.
BC021955 mRNA. Translation: AAH21955.1.
CCDSiCCDS57604.1. [Q9CZ42-3]
RefSeqiNP_001177286.1. NM_001190357.1.
NP_081271.2. NM_026995.4.
UniGeneiMm.267998.
Mm.64911.

Genome annotation databases

GeneIDi69225.
KEGGimmu:69225.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003048 mRNA. Translation: BAB22531.1.
AK009254 mRNA. Translation: BAB26172.1.
AK012456 mRNA. Translation: BAB28251.1.
AK013028 mRNA. Translation: BAB28607.1.
AK013069 mRNA. Translation: BAB28632.1.
AK013420 mRNA. Translation: BAB28847.1.
AK154142 mRNA. Translation: BAE32403.1.
AK171034 mRNA. Translation: BAE42200.1.
BC019538 mRNA. Translation: AAH19538.1.
BC021955 mRNA. Translation: AAH21955.1.
CCDSiCCDS57604.1. [Q9CZ42-3]
RefSeqiNP_001177286.1. NM_001190357.1.
NP_081271.2. NM_026995.4.
UniGeneiMm.267998.
Mm.64911.

3D structure databases

ProteinModelPortaliQ9CZ42.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CZ42. 3 interactors.
MINTiMINT-4126105.
STRINGi10090.ENSMUSP00000033901.

PTM databases

iPTMnetiQ9CZ42.
PhosphoSitePlusiQ9CZ42.
SwissPalmiQ9CZ42.

Proteomic databases

PaxDbiQ9CZ42.
PeptideAtlasiQ9CZ42.
PRIDEiQ9CZ42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi69225.
KEGGimmu:69225.

Organism-specific databases

CTDi69225.
MGIiMGI:1913353. Carkd.

Phylogenomic databases

eggNOGiKOG3974. Eukaryota.
COG0063. LUCA.
HOGENOMiHOG000163126.
HOVERGENiHBG057182.
InParanoidiQ9CZ42.
KOiK17757.
PhylomeDBiQ9CZ42.

Miscellaneous databases

ChiTaRSiCarkd. mouse.
PROiQ9CZ42.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031505.

Family and domain databases

CDDicd01171. YXKO-related. 1 hit.
Gene3Di3.40.1190.20. 1 hit.
HAMAPiMF_01965. NADHX_dehydratase. 1 hit.
InterProiIPR000631. CARKD.
IPR029056. Ribokinase-like.
[Graphical view]
PfamiPF01256. Carb_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00196. yjeF_cterm. 1 hit.
PROSITEiPS51383. YJEF_C_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNNRD_MOUSE
AccessioniPrimary (citable) accession number: Q9CZ42
Secondary accession number(s): Q9CQX9, Q9D7G7, Q9DC93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.