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Protein

Vacuolar-sorting protein SNF8

Gene

Snf8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the endosomal sorting complex required for transport II (ESCRT-II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. Required for degradation of both endocytosed EGF and EGFR, but not for the EGFR ligand-mediated internalization. Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transcription, Transcription regulation, Transport

Enzyme and pathway databases

ReactomeiR-MMU-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar-sorting protein SNF8
Alternative name(s):
ESCRT-II complex subunit VPS22
Gene namesi
Name:Snf8
Synonyms:D11Moh34
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1343161. Snf8.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Vacuolar-sorting protein SNF8PRO_0000215210Add
BLAST

Proteomic databases

EPDiQ9CZ28.
MaxQBiQ9CZ28.
PaxDbiQ9CZ28.
PeptideAtlasiQ9CZ28.
PRIDEiQ9CZ28.

PTM databases

iPTMnetiQ9CZ28.
PhosphoSiteiQ9CZ28.

Expressioni

Gene expression databases

BgeeiQ9CZ28.
ExpressionAtlasiQ9CZ28. baseline and differential.
GenevisibleiQ9CZ28. MM.

Interactioni

Subunit structurei

Component of the endosomal sorting complex required for transport II (ESCRT-II), composed of SNF8, VPS25 and VPS36. SNF8 is essential for the stability of the ESCRT-II complex. ESCRT-II interacts with ELL. Interacts with TSG101 (via the C-terminal domain). Interacts with RILPL1 (via the N-terminal domain); which recruits ESCRT-II to the endosome membranes. Interacts with 14-3-3 proteins (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205435. 4 interactions.
IntActiQ9CZ28. 5 interactions.
MINTiMINT-4126095.
STRINGi10090.ENSMUSP00000006217.

Structurei

3D structure databases

ProteinModelPortaliQ9CZ28.
SMRiQ9CZ28. Positions 34-252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili27 – 5327Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the SNF8 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG3341. Eukaryota.
ENOG410XPVM. LUCA.
GeneTreeiENSGT00390000007843.
HOGENOMiHOG000170930.
HOVERGENiHBG079872.
InParanoidiQ9CZ28.
KOiK12188.
OMAiWFPSLFP.
OrthoDBiEOG73FQNG.
PhylomeDBiQ9CZ28.
TreeFamiTF105950.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR007286. EAP30.
IPR016689. ESCRT-2_cplx_Snf8.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12806. PTHR12806. 1 hit.
PfamiPF04157. EAP30. 1 hit.
[Graphical view]
PIRSFiPIRSF017215. ESCRT2_Vps22. 1 hit.
SUPFAMiSSF46785. SSF46785. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9CZ28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRRGVGAGA IAKKKLAEAK YKERGTVLAE DQLAQMSKQL DMFKTNLEEF
60 70 80 90 100
ASKHKQEIRK NPEFRVQFQD MCATIGVDPL ASGKGFWSEM LGVGDFYYEL
110 120 130 140 150
GVQIIEVCLA LKHRNGGLIT LEELHQQVLK GRGKFAQDVS QDDLIRAIKK
160 170 180 190 200
LKALGTGFGI IPVGGTYLIQ SVPAELNMDH TVVLQLAEKN GYVTVSEIKT
210 220 230 240 250
SLKWETERAR QVLEHLLKEG LAWLDLQAPG EAHYWLPALF TDLYSQEISA

EEAKEAFP
Length:258
Mass (Da):28,886
Last modified:June 1, 2001 - v1
Checksum:iFD64947ED7815D5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013058 mRNA. Translation: BAB28626.1.
AL603682 Genomic DNA. Translation: CAM18269.1.
BC003938 mRNA. Translation: AAH03938.1.
CCDSiCCDS25288.1.
RefSeqiNP_291046.1. NM_033568.2.
UniGeneiMm.372235.

Genome annotation databases

EnsembliENSMUST00000006217; ENSMUSP00000006217; ENSMUSG00000006058.
GeneIDi27681.
KEGGimmu:27681.
UCSCiuc007lbb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013058 mRNA. Translation: BAB28626.1.
AL603682 Genomic DNA. Translation: CAM18269.1.
BC003938 mRNA. Translation: AAH03938.1.
CCDSiCCDS25288.1.
RefSeqiNP_291046.1. NM_033568.2.
UniGeneiMm.372235.

3D structure databases

ProteinModelPortaliQ9CZ28.
SMRiQ9CZ28. Positions 34-252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205435. 4 interactions.
IntActiQ9CZ28. 5 interactions.
MINTiMINT-4126095.
STRINGi10090.ENSMUSP00000006217.

PTM databases

iPTMnetiQ9CZ28.
PhosphoSiteiQ9CZ28.

Proteomic databases

EPDiQ9CZ28.
MaxQBiQ9CZ28.
PaxDbiQ9CZ28.
PeptideAtlasiQ9CZ28.
PRIDEiQ9CZ28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000006217; ENSMUSP00000006217; ENSMUSG00000006058.
GeneIDi27681.
KEGGimmu:27681.
UCSCiuc007lbb.1. mouse.

Organism-specific databases

CTDi11267.
MGIiMGI:1343161. Snf8.

Phylogenomic databases

eggNOGiKOG3341. Eukaryota.
ENOG410XPVM. LUCA.
GeneTreeiENSGT00390000007843.
HOGENOMiHOG000170930.
HOVERGENiHBG079872.
InParanoidiQ9CZ28.
KOiK12188.
OMAiWFPSLFP.
OrthoDBiEOG73FQNG.
PhylomeDBiQ9CZ28.
TreeFamiTF105950.

Enzyme and pathway databases

ReactomeiR-MMU-917729. Endosomal Sorting Complex Required For Transport (ESCRT).

Miscellaneous databases

PROiQ9CZ28.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CZ28.
ExpressionAtlasiQ9CZ28. baseline and differential.
GenevisibleiQ9CZ28. MM.

Family and domain databases

Gene3Di1.10.10.10. 2 hits.
InterProiIPR007286. EAP30.
IPR016689. ESCRT-2_cplx_Snf8.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR12806. PTHR12806. 1 hit.
PfamiPF04157. EAP30. 1 hit.
[Graphical view]
PIRSFiPIRSF017215. ESCRT2_Vps22. 1 hit.
SUPFAMiSSF46785. SSF46785. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Heart, Kidney, Liver, Lung, Pancreas and Spleen.

Entry informationi

Entry nameiSNF8_MOUSE
AccessioniPrimary (citable) accession number: Q9CZ28
Secondary accession number(s): A2A6M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.