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Protein

Glutaminyl-peptide cyclotransferase

Gene

Qpct

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue (By similarity).By similarity

Catalytic activityi

L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH3.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi160Zinc; catalyticBy similarity1
Active sitei202Proton acceptorBy similarity1
Metal bindingi203Zinc; catalyticBy similarity1
Active sitei249Proton acceptorBy similarity1
Metal bindingi331Zinc; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.2.5. 3474.

Protein family/group databases

MEROPSiM28.979.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminyl-peptide cyclotransferase (EC:2.3.2.5)
Alternative name(s):
Glutaminyl cyclase
Short name:
QC
Glutaminyl-tRNA cyclotransferase
Gene namesi
Name:Qpct
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1917786. Qpct.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 35Sequence analysisAdd BLAST35
ChainiPRO_000002219636 – 362Glutaminyl-peptide cyclotransferaseAdd BLAST327

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi29N-linked (GlcNAc...)Sequence analysis1
Glycosylationi50N-linked (GlcNAc...)1 Publication1
Disulfide bondi140 ↔ 1651 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9CYK2.
PeptideAtlasiQ9CYK2.
PRIDEiQ9CYK2.

PTM databases

PhosphoSitePlusiQ9CYK2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024084.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038732.

Chemistry databases

BindingDBiQ9CYK2.

Structurei

Secondary structure

1362
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi37 – 43Combined sources7
Helixi51 – 60Combined sources10
Helixi63 – 69Combined sources7
Helixi72 – 74Combined sources3
Helixi83 – 97Combined sources15
Beta strandi99 – 101Combined sources3
Beta strandi103 – 114Combined sources12
Beta strandi117 – 129Combined sources13
Beta strandi132 – 141Combined sources10
Beta strandi150 – 152Combined sources3
Turni158 – 161Combined sources4
Helixi162 – 174Combined sources13
Helixi176 – 180Combined sources5
Helixi181 – 183Combined sources3
Beta strandi192 – 200Combined sources9
Beta strandi205 – 207Combined sources3
Beta strandi209 – 213Combined sources5
Helixi215 – 225Combined sources11
Beta strandi227 – 230Combined sources4
Helixi238 – 241Combined sources4
Beta strandi242 – 250Combined sources9
Beta strandi253 – 255Combined sources3
Helixi263 – 265Combined sources3
Helixi266 – 281Combined sources16
Beta strandi285 – 287Combined sources3
Helixi290 – 292Combined sources3
Beta strandi298 – 300Combined sources3
Helixi309 – 312Combined sources4
Turni313 – 315Combined sources3
Beta strandi318 – 322Combined sources5
Turni328 – 331Combined sources4
Helixi337 – 339Combined sources3
Helixi342 – 360Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SI1X-ray2.90A36-362[»]
3SI2X-ray1.80A36-362[»]
ProteinModelPortaliQ9CYK2.
SMRiQ9CYK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3946. Eukaryota.
ENOG410YIYE. LUCA.
HOGENOMiHOG000189291.
HOVERGENiHBG009812.
InParanoidiQ9CYK2.
KOiK00683.
PhylomeDBiQ9CYK2.
TreeFamiTF315071.

Family and domain databases

InterProiIPR007484. Peptidase_M28.
[Graphical view]
PfamiPF04389. Peptidase_M28. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CYK2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGSEDKRVV GTLHLLLLQA TVLSLTAGNL SLVSAAWTQE KNHHQPAHLN
60 70 80 90 100
SSSLQQVAEG TSISEMWQND LRPLLIERYP GSPGSYSARQ HIMQRIQRLQ
110 120 130 140 150
AEWVVEVDTF LSRTPYGYRS FSNIISTLNP EAKRHLVLAC HYDSKYFPRW
160 170 180 190 200
DSRVFVGATD SAVPCAMMLE LARALDKKLH SLKDVSGSKP DLSLRLIFFD
210 220 230 240 250
GEEAFHHWSP QDSLYGSRHL AQKMASSPHP PGSRGTNQLD GMDLLVLLDL
260 270 280 290 300
IGAANPTFPN FFPKTTRWFN RLQAIEKELY ELGLLKDHSL ERKYFQNFGY
310 320 330 340 350
GNIIQDDHIP FLRKGVPVLH LIASPFPEVW HTMDDNEENL HASTIDNLNK
360
IIQVFVLEYL HL
Length:362
Mass (Da):41,119
Last modified:June 7, 2005 - v2
Checksum:iF23637D4EF6ED1F6
GO
Isoform 2 (identifier: Q9CYK2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-90: Missing.

Note: No experimental confirmation available.
Show »
Length:313
Mass (Da):35,648
Checksum:iB5FB17040CD91883
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8R → L in BAC29748 (PubMed:16141072).Curated1
Sequence conflicti8R → L in BAC32556 (PubMed:16141072).Curated1
Sequence conflicti8R → L in BAB30831 (PubMed:16141072).Curated1
Sequence conflicti31S → N in AAH20023 (PubMed:15489334).Curated1
Sequence conflicti35A → G in AAH20023 (PubMed:15489334).Curated1
Sequence conflicti213S → T in BAB30831 (PubMed:16141072).Curated1
Sequence conflicti326F → S in BAB30831 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01391142 – 90Missing in isoform 2. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037197 mRNA. Translation: BAC29748.1.
AK045974 mRNA. Translation: BAC32556.1.
AK017598 mRNA. Translation: BAB30831.1.
BC020023 mRNA. Translation: AAH20023.1.
CCDSiCCDS28983.1. [Q9CYK2-1]
RefSeqiNP_001303658.1. NM_001316729.1.
NP_081731.1. NM_027455.3.
UniGeneiMm.293870.

Genome annotation databases

GeneIDi70536.
KEGGimmu:70536.
UCSCiuc008dpv.1. mouse. [Q9CYK2-1]
uc008dpw.1. mouse. [Q9CYK2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037197 mRNA. Translation: BAC29748.1.
AK045974 mRNA. Translation: BAC32556.1.
AK017598 mRNA. Translation: BAB30831.1.
BC020023 mRNA. Translation: AAH20023.1.
CCDSiCCDS28983.1. [Q9CYK2-1]
RefSeqiNP_001303658.1. NM_001316729.1.
NP_081731.1. NM_027455.3.
UniGeneiMm.293870.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3SI1X-ray2.90A36-362[»]
3SI2X-ray1.80A36-362[»]
ProteinModelPortaliQ9CYK2.
SMRiQ9CYK2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038732.

Chemistry databases

BindingDBiQ9CYK2.

Protein family/group databases

MEROPSiM28.979.

PTM databases

PhosphoSitePlusiQ9CYK2.

Proteomic databases

PaxDbiQ9CYK2.
PeptideAtlasiQ9CYK2.
PRIDEiQ9CYK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi70536.
KEGGimmu:70536.
UCSCiuc008dpv.1. mouse. [Q9CYK2-1]
uc008dpw.1. mouse. [Q9CYK2-2]

Organism-specific databases

CTDi25797.
MGIiMGI:1917786. Qpct.

Phylogenomic databases

eggNOGiKOG3946. Eukaryota.
ENOG410YIYE. LUCA.
HOGENOMiHOG000189291.
HOVERGENiHBG009812.
InParanoidiQ9CYK2.
KOiK00683.
PhylomeDBiQ9CYK2.
TreeFamiTF315071.

Enzyme and pathway databases

BRENDAi2.3.2.5. 3474.

Miscellaneous databases

PROiQ9CYK2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024084.

Family and domain databases

InterProiIPR007484. Peptidase_M28.
[Graphical view]
PfamiPF04389. Peptidase_M28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQPCT_MOUSE
AccessioniPrimary (citable) accession number: Q9CYK2
Secondary accession number(s): Q8BH20, Q8VE07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.