Reviewed, UniProtKB/Swiss-Prot Q9CYK2 (QPCT_MOUSE)

Names and origin

Protein names

Recommended name:
    Glutaminyl-peptide cyclotransferase
    EC=2.3.2.5
Alternative name(s):
    Glutaminyl-tRNA cyclotransferase
      Short name=Glutaminyl cyclase
    QC

Gene names

Name:

Qpct

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue By similarity.
Catalytic activity	L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH3.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the glutaminyl-peptide cyclotransferase family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW glutaminyl-peptide cyclotransferase activity Inferred from electronic annotation. Source: EC peptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9CYK2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9CYK2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     42-90: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 362	327	Glutaminyl-peptide cyclotransferase		PRO_0000022196
Sites
	Active site	202	1	Proton acceptor By similarity
	Metal binding	160	1	Zinc By similarity
	Metal binding	203	1	Zinc By similarity
	Metal binding	331	1	Zinc By similarity
Amino acid modifications
	Glycosylation	29	1	N-linked (GlcNAc...) Potential
	Glycosylation	50	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	42 – 90	49	Missing in isoform 2.		VSP_013911
Experimental info
	Sequence conflict	8	1	R → L in BAC29748. Ref.1
	Sequence conflict	8	1	R → L in BAC32556. Ref.1
	Sequence conflict	8	1	R → L in BAB30831. Ref.1
	Sequence conflict	31	1	S → N in AAH20023. Ref.2
	Sequence conflict	35	1	A → G in AAH20023. Ref.2
	Sequence conflict	213	1	S → T in BAB30831. Ref.1
	Sequence conflict	326	1	F → S in BAB30831. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: F23637D4EF6ED1F6	FASTA	362	41,119
	10 20 30 40 50 60 MAGSEDKRVV GTLHLLLLQA TVLSLTAGNL SLVSAAWTQE KNHHQPAHLN SSSLQQVAEG 70 80 90 100 110 120 TSISEMWQND LRPLLIERYP GSPGSYSARQ HIMQRIQRLQ AEWVVEVDTF LSRTPYGYRS 130 140 150 160 170 180 FSNIISTLNP EAKRHLVLAC HYDSKYFPRW DSRVFVGATD SAVPCAMMLE LARALDKKLH 190 200 210 220 230 240 SLKDVSGSKP DLSLRLIFFD GEEAFHHWSP QDSLYGSRHL AQKMASSPHP PGSRGTNQLD 250 260 270 280 290 300 GMDLLVLLDL IGAANPTFPN FFPKTTRWFN RLQAIEKELY ELGLLKDHSL ERKYFQNFGY 310 320 330 340 350 360 GNIIQDDHIP FLRKGVPVLH LIASPFPEVW HTMDDNEENL HASTIDNLNK IIQVFVLEYL HL
	Isoform 2. Checksum: B5FB17040CD91883	FASTA	313	35,648
	10 20 30 40 50 60 MAGSEDKRVV GTLHLLLLQA TVLSLTAGNL SLVSAAWTQE KHIMQRIQRL QAEWVVEVDT 70 80 90 100 110 120 FLSRTPYGYR SFSNIISTLN PEAKRHLVLA CHYDSKYFPR WDSRVFVGAT DSAVPCAMML 130 140 150 160 170 180 ELARALDKKL HSLKDVSGSK PDLSLRLIFF DGEEAFHHWS PQDSLYGSRH LAQKMASSPH 190 200 210 220 230 240 PPGSRGTNQL DGMDLLVLLD LIGAANPTFP NFFPKTTRWF NRLQAIEKEL YELGLLKDHS 250 260 270 280 290 300 LERKYFQNFG YGNIIQDDHI PFLRKGVPVL HLIASPFPEV WHTMDDNEEN LHASTIDNLN 310 KIIQVFVLEY LHL

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Corpora quadrigemina, Embryo and Skin.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: Czech II. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK037197 mRNA. Translation: BAC29748.1. AK045974 mRNA. Translation: BAC32556.1. AK017598 mRNA. Translation: BAB30831.1. BC020023 mRNA. Translation: AAH20023.1.
IPI	IPI00461022. IPI00606704.
RefSeq	NP_081731.1.
UniGene	Mm.293870
3D structure databases
SMR	Q9CYK2. Positions 34-362.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9CYK2.
Protein family/group databases
MEROPS	M28.979.
Proteomic databases
PRIDE	Q9CYK2.
Genome annotation databases
Ensembl	ENSMUST00000040789; ENSMUSP00000038732; ENSMUSG00000024084; Mus musculus. [Genome view] ENSMUST00000112450; ENSMUSP00000108069; ENSMUSG00000024084; Mus musculus. [Genome view]
GeneID	70536.
KEGG	mmu:70536.
UCSC	uc008dpv.1. mouse. uc008dpw.1. mouse.
Organism-specific databases
CTD	70536.
MGI	MGI:1917786. Qpct.
Phylogenomic databases
HOGENOM	HBG314483.
HOVERGEN	Q9CYK2.
InParanoid	Q9CYK2.
PhylomeDB	Q9CYK2.
Enzyme and pathway databases
BRENDA	2.3.2.5. 244.
Gene expression databases
ArrayExpress	Q9CYK2.
Bgee	Q9CYK2.
Genevestigator	Q9CYK2.
GermOnline	ENSMUSG00000024084. Mus musculus.
Family and domain databases
InterPro	IPR007484. Peptidase_M28. [Graphical view]
Pfam	PF04389. Peptidase_M28. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	331811.
SOURCE	Search...

Entry information

Entry name

QPCT_MOUSE

Accession

Primary (citable) accession number: Q9CYK2
Secondary accession number(s): Q8BH20, Q8VE07

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	January 19, 2010
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)