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Protein

Glutaminyl-peptide cyclotransferase

Gene

Qpct

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue (By similarity).By similarity

Catalytic activityi

L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH3.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi160 – 1601Zinc; catalyticBy similarity
Active sitei202 – 2021Proton acceptorBy similarity
Metal bindingi203 – 2031Zinc; catalyticBy similarity
Active sitei249 – 2491Proton acceptorBy similarity
Metal bindingi331 – 3311Zinc; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.3.2.5. 3474.

Protein family/group databases

MEROPSiM28.979.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminyl-peptide cyclotransferase (EC:2.3.2.5)
Alternative name(s):
Glutaminyl cyclase
Short name:
QC
Glutaminyl-tRNA cyclotransferase
Gene namesi
Name:Qpct
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1917786. Qpct.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Sequence analysisAdd
BLAST
Chaini36 – 362327Glutaminyl-peptide cyclotransferasePRO_0000022196Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi29 – 291N-linked (GlcNAc...)Sequence analysis
Glycosylationi50 – 501N-linked (GlcNAc...)1 Publication
Disulfide bondi140 ↔ 1651 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9CYK2.
PaxDbiQ9CYK2.
PeptideAtlasiQ9CYK2.
PRIDEiQ9CYK2.

PTM databases

PhosphoSiteiQ9CYK2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000024084.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038732.

Chemistry

BindingDBiQ9CYK2.

Structurei

Secondary structure

1
362
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi37 – 437Combined sources
Helixi51 – 6010Combined sources
Helixi63 – 697Combined sources
Helixi72 – 743Combined sources
Helixi83 – 9715Combined sources
Beta strandi99 – 1013Combined sources
Beta strandi103 – 11412Combined sources
Beta strandi117 – 12913Combined sources
Beta strandi132 – 14110Combined sources
Beta strandi150 – 1523Combined sources
Turni158 – 1614Combined sources
Helixi162 – 17413Combined sources
Helixi176 – 1805Combined sources
Helixi181 – 1833Combined sources
Beta strandi192 – 2009Combined sources
Beta strandi205 – 2073Combined sources
Beta strandi209 – 2135Combined sources
Helixi215 – 22511Combined sources
Beta strandi227 – 2304Combined sources
Helixi238 – 2414Combined sources
Beta strandi242 – 2509Combined sources
Beta strandi253 – 2553Combined sources
Helixi263 – 2653Combined sources
Helixi266 – 28116Combined sources
Beta strandi285 – 2873Combined sources
Helixi290 – 2923Combined sources
Beta strandi298 – 3003Combined sources
Helixi309 – 3124Combined sources
Turni313 – 3153Combined sources
Beta strandi318 – 3225Combined sources
Turni328 – 3314Combined sources
Helixi337 – 3393Combined sources
Helixi342 – 36019Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SI1X-ray2.90A36-362[»]
3SI2X-ray1.80A36-362[»]
ProteinModelPortaliQ9CYK2.
SMRiQ9CYK2. Positions 36-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3946. Eukaryota.
ENOG410YIYE. LUCA.
HOGENOMiHOG000189291.
HOVERGENiHBG009812.
InParanoidiQ9CYK2.
KOiK00683.
PhylomeDBiQ9CYK2.
TreeFamiTF315071.

Family and domain databases

InterProiIPR007484. Peptidase_M28.
[Graphical view]
PfamiPF04389. Peptidase_M28. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CYK2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGSEDKRVV GTLHLLLLQA TVLSLTAGNL SLVSAAWTQE KNHHQPAHLN
60 70 80 90 100
SSSLQQVAEG TSISEMWQND LRPLLIERYP GSPGSYSARQ HIMQRIQRLQ
110 120 130 140 150
AEWVVEVDTF LSRTPYGYRS FSNIISTLNP EAKRHLVLAC HYDSKYFPRW
160 170 180 190 200
DSRVFVGATD SAVPCAMMLE LARALDKKLH SLKDVSGSKP DLSLRLIFFD
210 220 230 240 250
GEEAFHHWSP QDSLYGSRHL AQKMASSPHP PGSRGTNQLD GMDLLVLLDL
260 270 280 290 300
IGAANPTFPN FFPKTTRWFN RLQAIEKELY ELGLLKDHSL ERKYFQNFGY
310 320 330 340 350
GNIIQDDHIP FLRKGVPVLH LIASPFPEVW HTMDDNEENL HASTIDNLNK
360
IIQVFVLEYL HL
Length:362
Mass (Da):41,119
Last modified:June 7, 2005 - v2
Checksum:iF23637D4EF6ED1F6
GO
Isoform 2 (identifier: Q9CYK2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     42-90: Missing.

Note: No experimental confirmation available.
Show »
Length:313
Mass (Da):35,648
Checksum:iB5FB17040CD91883
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81R → L in BAC29748 (PubMed:16141072).Curated
Sequence conflicti8 – 81R → L in BAC32556 (PubMed:16141072).Curated
Sequence conflicti8 – 81R → L in BAB30831 (PubMed:16141072).Curated
Sequence conflicti31 – 311S → N in AAH20023 (PubMed:15489334).Curated
Sequence conflicti35 – 351A → G in AAH20023 (PubMed:15489334).Curated
Sequence conflicti213 – 2131S → T in BAB30831 (PubMed:16141072).Curated
Sequence conflicti326 – 3261F → S in BAB30831 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei42 – 9049Missing in isoform 2. 1 PublicationVSP_013911Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037197 mRNA. Translation: BAC29748.1.
AK045974 mRNA. Translation: BAC32556.1.
AK017598 mRNA. Translation: BAB30831.1.
BC020023 mRNA. Translation: AAH20023.1.
CCDSiCCDS28983.1. [Q9CYK2-1]
RefSeqiNP_001303658.1. NM_001316729.1.
NP_081731.1. NM_027455.3.
UniGeneiMm.293870.

Genome annotation databases

GeneIDi70536.
KEGGimmu:70536.
UCSCiuc008dpv.1. mouse. [Q9CYK2-1]
uc008dpw.1. mouse. [Q9CYK2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK037197 mRNA. Translation: BAC29748.1.
AK045974 mRNA. Translation: BAC32556.1.
AK017598 mRNA. Translation: BAB30831.1.
BC020023 mRNA. Translation: AAH20023.1.
CCDSiCCDS28983.1. [Q9CYK2-1]
RefSeqiNP_001303658.1. NM_001316729.1.
NP_081731.1. NM_027455.3.
UniGeneiMm.293870.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3SI1X-ray2.90A36-362[»]
3SI2X-ray1.80A36-362[»]
ProteinModelPortaliQ9CYK2.
SMRiQ9CYK2. Positions 36-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000038732.

Chemistry

BindingDBiQ9CYK2.

Protein family/group databases

MEROPSiM28.979.

PTM databases

PhosphoSiteiQ9CYK2.

Proteomic databases

MaxQBiQ9CYK2.
PaxDbiQ9CYK2.
PeptideAtlasiQ9CYK2.
PRIDEiQ9CYK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi70536.
KEGGimmu:70536.
UCSCiuc008dpv.1. mouse. [Q9CYK2-1]
uc008dpw.1. mouse. [Q9CYK2-2]

Organism-specific databases

CTDi25797.
MGIiMGI:1917786. Qpct.

Phylogenomic databases

eggNOGiKOG3946. Eukaryota.
ENOG410YIYE. LUCA.
HOGENOMiHOG000189291.
HOVERGENiHBG009812.
InParanoidiQ9CYK2.
KOiK00683.
PhylomeDBiQ9CYK2.
TreeFamiTF315071.

Enzyme and pathway databases

BRENDAi2.3.2.5. 3474.

Miscellaneous databases

PROiQ9CYK2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024084.

Family and domain databases

InterProiIPR007484. Peptidase_M28.
[Graphical view]
PfamiPF04389. Peptidase_M28. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiQPCT_MOUSE
AccessioniPrimary (citable) accession number: Q9CYK2
Secondary accession number(s): Q8BH20, Q8VE07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: September 7, 2016
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.