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Protein

Tryptophan--tRNA ligase, mitochondrial

Gene

Wars2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42ATPBy similarity1
Binding sitei167L-tryptophanBy similarity1
Binding sitei217ATP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 51ATPBy similarity4
Nucleotide bindingi179 – 181ATPBy similarity3
Nucleotide bindingi226 – 230ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

  • tryptophanyl-tRNA aminoacylation Source: InterPro
  • vasculogenesis Source: MGI

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, mitochondrial (EC:6.1.1.2)
Alternative name(s):
(Mt)TrpRS
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:Wars2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1917810. Wars2.

Subcellular locationi

  • Mitochondrion matrix By similarity

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB
  • mitochondrion Source: MGI
  • plasma membrane Source: MGI

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 18MitochondrionBy similarityAdd BLAST18
ChainiPRO_000003582919 – 360Tryptophan--tRNA ligase, mitochondrialAdd BLAST342

Proteomic databases

EPDiQ9CYK1.
MaxQBiQ9CYK1.
PaxDbiQ9CYK1.
PeptideAtlasiQ9CYK1.
PRIDEiQ9CYK1.

PTM databases

iPTMnetiQ9CYK1.
PhosphoSitePlusiQ9CYK1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004233.
CleanExiMM_WARS2.
ExpressionAtlasiQ9CYK1. baseline and differential.
GenevisibleiQ9CYK1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004343.

Structurei

3D structure databases

ProteinModelPortaliQ9CYK1.
SMRiQ9CYK1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2713. Eukaryota.
COG0180. LUCA.
GeneTreeiENSGT00510000047425.
HOGENOMiHOG000059940.
HOVERGENiHBG027444.
InParanoidiQ9CYK1.
KOiK01867.
OMAiYLGAIKH.
OrthoDBiEOG091G04TZ.
TreeFamiTF314321.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProiView protein in InterPro
IPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
PfamiView protein in Pfam
PF00579. tRNA-synt_1b. 1 hit.
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CYK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALFSVRKAR ECWRFIRALH KGPAATLAPQ KESGERVFSG IQPTGILHLG
60 70 80 90 100
NYLGAIESWV NLQEEYDTVI YSIVDLHSIT VPQDPTVLQQ SILDMTAVLL
110 120 130 140 150
ACGINPEKSI LFQQSKVSEH TQLSWILTCM VRLPRLQHLH QWKAKAAKQK
160 170 180 190 200
HDGTVGLLTY PVLQAADILC YKSTHVPVGE DQVQHMELVQ DLARSFNQKY
210 220 230 240 250
GEFFPLPKSI LTSMKKVKSL RDPSSKMSKS DPDKLATVRI TDSPEEIVQK
260 270 280 290 300
FRKAVTDFTS EVTYEPDSRA GVSNMVAIHA AVSGLSVEEV VRSSAGLDTA
310 320 330 340 350
RYKLLVADAV IEKFAPIRKE IEKLKMDKDH LRKVLLVGSA KAKELASPVF
360
EEVKKLVGIL
Length:360
Mass (Da):40,153
Last modified:July 27, 2011 - v2
Checksum:iE777D70B3827807A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti148 – 149KQ → NR in BAB30833 (PubMed:16141072).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017602 mRNA. Translation: BAB30833.1.
AK042715 mRNA. Translation: BAC31342.1.
AK042964 mRNA. Translation: BAC31422.1.
BC089583 mRNA. Translation: AAH89583.1.
CCDSiCCDS17672.1.
RefSeqiNP_081738.2. NM_027462.4.
UniGeneiMm.316754.

Genome annotation databases

EnsembliENSMUST00000004343; ENSMUSP00000004343; ENSMUSG00000004233.
GeneIDi70560.
KEGGimmu:70560.
UCSCiuc008qqk.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSYWM_MOUSE
AccessioniPrimary (citable) accession number: Q9CYK1
Secondary accession number(s): Q8BFV8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: July 27, 2011
Last modified: July 5, 2017
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families