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Protein

Tryptophan--tRNA ligase, mitochondrial

Gene

Wars2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. tryptophan-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: InterPro
  2. vasculogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligase, mitochondrial (EC:6.1.1.2)
Alternative name(s):
(Mt)TrpRS
Tryptophanyl-tRNA synthetase
Short name:
TrpRS
Gene namesi
Name:Wars2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1917810. Wars2.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 1818MitochondrionBy similarityAdd
BLAST
Chaini19 – 360342Tryptophan--tRNA ligase, mitochondrialPRO_0000035829Add
BLAST

Proteomic databases

MaxQBiQ9CYK1.
PaxDbiQ9CYK1.
PRIDEiQ9CYK1.

PTM databases

PhosphoSiteiQ9CYK1.

Expressioni

Gene expression databases

BgeeiQ9CYK1.
CleanExiMM_WARS2.
GenevestigatoriQ9CYK1.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004343.

Structurei

3D structure databases

ProteinModelPortaliQ9CYK1.
SMRiQ9CYK1. Positions 37-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0180.
GeneTreeiENSGT00510000047425.
HOGENOMiHOG000059940.
HOVERGENiHBG027444.
InParanoidiQ9CYK1.
KOiK01867.
OMAiGWGQFKP.
OrthoDBiEOG7J447N.
TreeFamiTF314321.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CYK1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALFSVRKAR ECWRFIRALH KGPAATLAPQ KESGERVFSG IQPTGILHLG
60 70 80 90 100
NYLGAIESWV NLQEEYDTVI YSIVDLHSIT VPQDPTVLQQ SILDMTAVLL
110 120 130 140 150
ACGINPEKSI LFQQSKVSEH TQLSWILTCM VRLPRLQHLH QWKAKAAKQK
160 170 180 190 200
HDGTVGLLTY PVLQAADILC YKSTHVPVGE DQVQHMELVQ DLARSFNQKY
210 220 230 240 250
GEFFPLPKSI LTSMKKVKSL RDPSSKMSKS DPDKLATVRI TDSPEEIVQK
260 270 280 290 300
FRKAVTDFTS EVTYEPDSRA GVSNMVAIHA AVSGLSVEEV VRSSAGLDTA
310 320 330 340 350
RYKLLVADAV IEKFAPIRKE IEKLKMDKDH LRKVLLVGSA KAKELASPVF
360
EEVKKLVGIL
Length:360
Mass (Da):40,153
Last modified:July 26, 2011 - v2
Checksum:iE777D70B3827807A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti148 – 1492KQ → NR in BAB30833 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017602 mRNA. Translation: BAB30833.1.
AK042715 mRNA. Translation: BAC31342.1.
AK042964 mRNA. Translation: BAC31422.1.
BC089583 mRNA. Translation: AAH89583.1.
CCDSiCCDS17672.1.
RefSeqiNP_081738.2. NM_027462.4.
UniGeneiMm.316754.

Genome annotation databases

EnsembliENSMUST00000004343; ENSMUSP00000004343; ENSMUSG00000004233.
GeneIDi70560.
KEGGimmu:70560.
UCSCiuc008qqk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017602 mRNA. Translation: BAB30833.1.
AK042715 mRNA. Translation: BAC31342.1.
AK042964 mRNA. Translation: BAC31422.1.
BC089583 mRNA. Translation: AAH89583.1.
CCDSiCCDS17672.1.
RefSeqiNP_081738.2. NM_027462.4.
UniGeneiMm.316754.

3D structure databases

ProteinModelPortaliQ9CYK1.
SMRiQ9CYK1. Positions 37-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000004343.

PTM databases

PhosphoSiteiQ9CYK1.

Proteomic databases

MaxQBiQ9CYK1.
PaxDbiQ9CYK1.
PRIDEiQ9CYK1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004343; ENSMUSP00000004343; ENSMUSG00000004233.
GeneIDi70560.
KEGGimmu:70560.
UCSCiuc008qqk.1. mouse.

Organism-specific databases

CTDi10352.
MGIiMGI:1917810. Wars2.

Phylogenomic databases

eggNOGiCOG0180.
GeneTreeiENSGT00510000047425.
HOGENOMiHOG000059940.
HOVERGENiHBG027444.
InParanoidiQ9CYK1.
KOiK01867.
OMAiGWGQFKP.
OrthoDBiEOG7J447N.
TreeFamiTF314321.

Miscellaneous databases

NextBioi331864.
PROiQ9CYK1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CYK1.
CleanExiMM_WARS2.
GenevestigatoriQ9CYK1.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum and Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiSYWM_MOUSE
AccessioniPrimary (citable) accession number: Q9CYK1
Secondary accession number(s): Q8BFV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 5, 2002
Last sequence update: July 26, 2011
Last modified: January 6, 2015
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.