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Protein

Mitochondrial import receptor subunit TOM34

Gene

Tomm34

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM34
Alternative name(s):
Translocase of outer membrane 34 kDa subunit
Gene namesi
Name:Tomm34
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1914395. Tomm34.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • membrane Source: MGI
  • mitochondrial outer membrane Source: MGI
  • mitochondrion Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable, grow normally and both males and females are fertile. There is no effect on spermatogenesis or protein import into mitochondria.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Mitochondrial import receptor subunit TOM34PRO_0000106335Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei8 – 81PhosphoserineCombined sources
Modified residuei160 – 1601PhosphoserineBy similarity
Modified residuei186 – 1861PhosphoserineCombined sources
Isoform 2 (identifier: Q9CYG7-2)
Modified residuei8 – 81PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9CYG7.
MaxQBiQ9CYG7.
PaxDbiQ9CYG7.
PeptideAtlasiQ9CYG7.
PRIDEiQ9CYG7.

PTM databases

iPTMnetiQ9CYG7.
PhosphoSiteiQ9CYG7.

Expressioni

Tissue specificityi

Isoform 1 is ubiquitously expressed while isoform 2 is expressed only in mature testicular germ cells. Isoform 1 is expressed in all testicular cells. Isoform 2 is highly expressed in early to late pachytene cells but expression is significantly decreased in round spermatid cells.1 Publication

Developmental stagei

Isoform 2 is hardly detectable in testis at 1 week post partum but expression gradually increases for 2 to 4 weeks post partum. Isoform 1 is detected during this period but its expression does not change.1 Publication

Gene expression databases

BgeeiENSMUSG00000018322.
CleanExiMM_TOMM34.
GenevisibleiQ9CYG7. MM.

Interactioni

Subunit structurei

Interacts with HSP90A, VCP, ATP6M, KIAA0665, AMPK, and DMAP1 through its TPR repeat.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211975. 1 interaction.
IntActiQ9CYG7. 1 interaction.
STRINGi10090.ENSMUSP00000018466.

Structurei

3D structure databases

ProteinModelPortaliQ9CYG7.
SMRiQ9CYG7. Positions 11-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati9 – 4234TPR 1Add
BLAST
Repeati51 – 8434TPR 2Add
BLAST
Repeati85 – 11834TPR 3Add
BLAST
Repeati193 – 22634TPR 4Add
BLAST
Repeati227 – 26034TPR 5Add
BLAST
Repeati261 – 29434TPR 6Add
BLAST

Sequence similaritiesi

Belongs to the Tom34 family.Curated
Contains 6 TPR repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00840000129761.
HOVERGENiHBG105545.
InParanoidiQ9CYG7.
KOiK17766.
OMAiNCNECIK.
OrthoDBiEOG091G0FFR.
PhylomeDBiQ9CYG7.
TreeFamiTF106202.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Isoforms 1 and 2 are produced by 2 alternative initial coding exons with very similar sequences which differ at only 4 positions.
Isoform 1 (identifier: Q9CYG7-1) [UniParc]FASTAAdd to basket
Also known as: Tomm34a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPKVSDSVE QLRAAGNQNF RNGQYGEASA LYERALRLLQ ARGSADPEEE
60 70 80 90 100
SVLYSNRAAC YLKDGNCTDC IKDCTSALAL VPFSIKPLLR RASAYEALEK
110 120 130 140 150
YALAYVDYKT VLQIDNSVAS ALEGINRITR ALMDSLGPEW RLKLPPIPVV
160 170 180 190 200
PVSAQKRWNS LPSDNHKETA KTKSKEATAT KSRVPSAGDV ERAKALKEEG
210 220 230 240 250
NDLVKKGNHK KAIEKYSESL LCSSLESATY SNRALCHLVL KQYKEAVKDC
260 270 280 290 300
TEALKLDGKN VKAFYRRAQA YKALKDYKSS LSDISSLLQI EPRNGPAQKL

RQEVNQNMN
Length:309
Mass (Da):34,278
Last modified:June 1, 2001 - v1
Checksum:i4367551F4063278A
GO
Isoform 2 (identifier: Q9CYG7-2) [UniParc]FASTAAdd to basket
Also known as: Tomm34b

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: MAPKVSDSVEQLRAAGNQNFRNGQYG → MAPKLSDSVEELRAAGNQSFRNGQYA

Show »
Length:309
Mass (Da):34,280
Checksum:i3DC7545AC92EA6AC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti59 – 591A → S in BAC57494 (PubMed:12801914).Curated
Sequence conflicti59 – 591A → S in BAC57495 (PubMed:12801914).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2626MAPKV…NGQYG → MAPKLSDSVEELRAAGNQSF RNGQYA in isoform 2. 1 PublicationVSP_035044Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB087254 Genomic DNA. Translation: BAC57494.1.
AB087254 Genomic DNA. Translation: BAC57495.1.
AK011786 mRNA. Translation: BAB27840.1.
AK017699 mRNA. Translation: BAB30882.1.
AK075873 mRNA. Translation: BAC36020.1.
AK168329 mRNA. Translation: BAE40267.1.
AL591542 Genomic DNA. Translation: CAM20764.1.
AL591542 Genomic DNA. Translation: CAM20765.1.
BC018278 mRNA. Translation: AAH18278.1.
CCDSiCCDS17020.1. [Q9CYG7-2]
CCDS71187.1. [Q9CYG7-1]
RefSeqiNP_001278084.1. NM_001291155.1. [Q9CYG7-1]
NP_080272.1. NM_025996.5. [Q9CYG7-2]
XP_006500130.1. XM_006500067.1. [Q9CYG7-2]
UniGeneiMm.23173.

Genome annotation databases

EnsembliENSMUST00000018466; ENSMUSP00000018466; ENSMUSG00000018322. [Q9CYG7-2]
ENSMUST00000109384; ENSMUSP00000105010; ENSMUSG00000018322. [Q9CYG7-1]
GeneIDi67145.
KEGGimmu:67145.
UCSCiuc008ntr.2. mouse. [Q9CYG7-2]
uc008nts.2. mouse. [Q9CYG7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB087254 Genomic DNA. Translation: BAC57494.1.
AB087254 Genomic DNA. Translation: BAC57495.1.
AK011786 mRNA. Translation: BAB27840.1.
AK017699 mRNA. Translation: BAB30882.1.
AK075873 mRNA. Translation: BAC36020.1.
AK168329 mRNA. Translation: BAE40267.1.
AL591542 Genomic DNA. Translation: CAM20764.1.
AL591542 Genomic DNA. Translation: CAM20765.1.
BC018278 mRNA. Translation: AAH18278.1.
CCDSiCCDS17020.1. [Q9CYG7-2]
CCDS71187.1. [Q9CYG7-1]
RefSeqiNP_001278084.1. NM_001291155.1. [Q9CYG7-1]
NP_080272.1. NM_025996.5. [Q9CYG7-2]
XP_006500130.1. XM_006500067.1. [Q9CYG7-2]
UniGeneiMm.23173.

3D structure databases

ProteinModelPortaliQ9CYG7.
SMRiQ9CYG7. Positions 11-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211975. 1 interaction.
IntActiQ9CYG7. 1 interaction.
STRINGi10090.ENSMUSP00000018466.

PTM databases

iPTMnetiQ9CYG7.
PhosphoSiteiQ9CYG7.

Proteomic databases

EPDiQ9CYG7.
MaxQBiQ9CYG7.
PaxDbiQ9CYG7.
PeptideAtlasiQ9CYG7.
PRIDEiQ9CYG7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018466; ENSMUSP00000018466; ENSMUSG00000018322. [Q9CYG7-2]
ENSMUST00000109384; ENSMUSP00000105010; ENSMUSG00000018322. [Q9CYG7-1]
GeneIDi67145.
KEGGimmu:67145.
UCSCiuc008ntr.2. mouse. [Q9CYG7-2]
uc008nts.2. mouse. [Q9CYG7-1]

Organism-specific databases

CTDi10953.
MGIiMGI:1914395. Tomm34.

Phylogenomic databases

eggNOGiKOG1124. Eukaryota.
COG0457. LUCA.
GeneTreeiENSGT00840000129761.
HOVERGENiHBG105545.
InParanoidiQ9CYG7.
KOiK17766.
OMAiNCNECIK.
OrthoDBiEOG091G0FFR.
PhylomeDBiQ9CYG7.
TreeFamiTF106202.

Miscellaneous databases

ChiTaRSiTomm34. mouse.
PROiQ9CYG7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018322.
CleanExiMM_TOMM34.
GenevisibleiQ9CYG7. MM.

Family and domain databases

Gene3Di1.25.40.10. 2 hits.
InterProiIPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical_dom.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF00515. TPR_1. 1 hit.
PF13181. TPR_8. 1 hit.
[Graphical view]
SMARTiSM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 2 hits.
PROSITEiPS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOM34_MOUSE
AccessioniPrimary (citable) accession number: Q9CYG7
Secondary accession number(s): A2A5N5
, A2A5N6, Q3THD0, Q812F1, Q812F2, Q9D059
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.