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Q9CYC6

- DCP2_MOUSE

UniProt

Q9CYC6 - DCP2_MOUSE

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Protein
m7GpppN-mRNA hydrolase
Gene
Dcp2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking an RNA moiety.1 Publication

Catalytic activityi

5'-(N(7)-methylguanosine 5'-triphospho)-(mRNA) + H2O = N(7)-methylguanosine 5'-diphosphate + 5'-phospho-(mRNA).

Cofactori

Manganese. Required for highest activity. Can also utilize magnesium ions By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi144 – 1441Manganese By similarity
Metal bindingi148 – 1481Manganese By similarity

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
  2. m7G(5')pppN diphosphatase activity Source: UniProtKB
  3. manganese ion binding Source: InterPro

GO - Biological processi

  1. histone mRNA catabolic process Source: UniProtKB
  2. mRNA catabolic process Source: UniProtKB
  3. nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nonsense-mediated mRNA decay

Keywords - Ligandi

Manganese, Metal-binding, RNA-binding

Enzyme and pathway databases

ReactomeiREACT_198696. KSRP destabilizes mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
m7GpppN-mRNA hydrolase (EC:3.6.1.62)
Alternative name(s):
mRNA-decapping enzyme 2
Gene namesi
Name:Dcp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:1917890. Dcp2.

Subcellular locationi

CytoplasmP-body By similarity. Nucleus By similarity
Note: Predominantly cytoplasmic, in processing bodies (PB). A minor amount is nuclear By similarity.

GO - Cellular componenti

  1. RISC complex Source: Ensembl
  2. cytoplasmic mRNA processing body Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 422422m7GpppN-mRNA hydrolase
PRO_0000057052Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei246 – 2461Phosphoserine1 Publication
Modified residuei247 – 2471Phosphoserine1 Publication
Modified residuei249 – 2491Phosphoserine1 Publication
Modified residuei276 – 2761Phosphoserine By similarity
Modified residuei284 – 2841Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9CYC6.
PRIDEiQ9CYC6.

PTM databases

PhosphoSiteiQ9CYC6.

Expressioni

Tissue specificityi

Strongly expressed in brain and testis. Weakly expressed in lung. Not detected in heart, liver, kidney and muscle (at protein level).1 Publication

Developmental stagei

Strongly expressed in brain, heart, liver at 14.5 and 16.5 dpc. Strongly expressed in brain at 20 dpc. Weakly expressed in heart and liver at 20 dpc (at protein level).1 Publication

Gene expression databases

BgeeiQ9CYC6.
CleanExiMM_DCP2.
GenevestigatoriQ9CYC6.

Interactioni

Subunit structurei

Found in a mRNA decay complex with LSM1, LSM3, LSM4, EXOSC2, EXOSC4, EXOSC10, PARN, XRN1, CNOT6, UPF1, UPF2 and UPF3B. Forms a complex with DCP1A, EDC3, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC4/HEDLS. Interacts with DPC1B, UPF1, UPF2 and UPF3B. Associates with polysomes. Interacts (via N-terminus and C-terminus) with TRIM21 (via N-terminus and C-terminus) By similarity. Interacts with LIMD1, WTIP and AJUBA By similarity. Interacts with DDX17 in an RNA-dependent manner By similarity. Interacts with ZC3HAV1 By similarity. Interacts with APOBEC3G in an RNA-dependent manner By similarity.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025350.

Structurei

3D structure databases

ProteinModelPortaliQ9CYC6.
SMRiQ9CYC6. Positions 12-242.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini95 – 226132Nudix hydrolase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi129 – 15022Nudix box
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0494.
GeneTreeiENSGT00390000018878.
HOGENOMiHOG000005974.
HOVERGENiHBG051321.
InParanoidiQ3TLD9.
KOiK12613.
OMAiIINCTIY.
OrthoDBiEOG7XM2XP.
TreeFamiTF314180.

Family and domain databases

Gene3Di3.90.79.10. 1 hit.
InterProiIPR007722. mRNA_decapping_BoxA.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view]
PfamiPF05026. DCP2. 1 hit.
PF00293. NUDIX. 1 hit.
[Graphical view]
SUPFAMiSSF55811. SSF55811. 1 hit.
PROSITEiPS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CYC6-1 [UniParc]FASTAAdd to Basket

« Hide

MEPKRLEIPG SVLDDLCSRF ILHIPSEERD NAIRVCFQIE LAHWFYLDFY    50
MQNTPGLPQC GIRDFAKAVF SHCPFLLPQG EDVEKILDEW KEYKMGVPTY 100
GAIILDETLE NVLLVQGYLA KSGWGFPKGK VNKEEAPHDC AAREVFEETG 150
FDIKDYICKD DYIELRINDQ LARLYIIPGV PKDTKFNPKT RREIRNIEWF 200
SIEKLPCHRN DMTPKSKLGL APNKFFMAIP FIRPLRDWLS RRFGDSSDSD 250
NGFSSAGSTP ARPTVEKLSR TKFRHSQQLF PEGSPSDQWV KHRQPLQQKS 300
HSNHGEVSDL LKAKNQNMRG NGRKQYQDSP NQKKRANGVH GQPAKQQNPL 350
VKCEKKLHPR KLQDNFETDA TCDLPCSGEE PSVEHAEGHS VACNGHCKFP 400
FSSRAFLSFK FDQNAIMKIL DL 422
Length:422
Mass (Da):48,380
Last modified:July 27, 2011 - v2
Checksum:i80FE791B05E4C8FD
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti226 – 2261F → Y in BAB30946. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK017809 mRNA. Translation: BAB30946.1.
AK166560 mRNA. Translation: BAE38853.1.
CCDSiCCDS50275.1.
RefSeqiNP_081766.1. NM_027490.1.
UniGeneiMm.87629.

Genome annotation databases

EnsembliENSMUST00000025350; ENSMUSP00000025350; ENSMUSG00000024472.
GeneIDi70640.
KEGGimmu:70640.
UCSCiuc008eux.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK017809 mRNA. Translation: BAB30946.1 .
AK166560 mRNA. Translation: BAE38853.1 .
CCDSi CCDS50275.1.
RefSeqi NP_081766.1. NM_027490.1.
UniGenei Mm.87629.

3D structure databases

ProteinModelPortali Q9CYC6.
SMRi Q9CYC6. Positions 12-242.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000025350.

PTM databases

PhosphoSitei Q9CYC6.

Proteomic databases

PaxDbi Q9CYC6.
PRIDEi Q9CYC6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000025350 ; ENSMUSP00000025350 ; ENSMUSG00000024472 .
GeneIDi 70640.
KEGGi mmu:70640.
UCSCi uc008eux.2. mouse.

Organism-specific databases

CTDi 167227.
MGIi MGI:1917890. Dcp2.

Phylogenomic databases

eggNOGi COG0494.
GeneTreei ENSGT00390000018878.
HOGENOMi HOG000005974.
HOVERGENi HBG051321.
InParanoidi Q3TLD9.
KOi K12613.
OMAi IINCTIY.
OrthoDBi EOG7XM2XP.
TreeFami TF314180.

Enzyme and pathway databases

Reactomei REACT_198696. KSRP destabilizes mRNA.

Miscellaneous databases

NextBioi 332021.
PROi Q9CYC6.
SOURCEi Search...

Gene expression databases

Bgeei Q9CYC6.
CleanExi MM_DCP2.
Genevestigatori Q9CYC6.

Family and domain databases

Gene3Di 3.90.79.10. 1 hit.
InterProi IPR007722. mRNA_decapping_BoxA.
IPR020084. NUDIX_hydrolase_CS.
IPR000086. NUDIX_hydrolase_dom.
IPR015797. NUDIX_hydrolase_dom-like.
[Graphical view ]
Pfami PF05026. DCP2. 1 hit.
PF00293. NUDIX. 1 hit.
[Graphical view ]
SUPFAMi SSF55811. SSF55811. 1 hit.
PROSITEi PS51462. NUDIX. 1 hit.
PS00893. NUDIX_BOX. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-246; SER-247 AND SER-249, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  3. "Multiple mRNA decapping enzymes in mammalian cells."
    Song M.G., Li Y., Kiledjian M.
    Mol. Cell 40:423-432(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A DECAPPING ENZYME, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiDCP2_MOUSE
AccessioniPrimary (citable) accession number: Q9CYC6
Secondary accession number(s): Q3TLD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi