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Protein

H/ACA ribonucleoprotein complex subunit 1

Gene

Gar1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRibonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex subunit 1
Alternative name(s):
Nucleolar protein family A member 1
snoRNP protein GAR1
Gene namesi
Name:Gar1
Synonyms:Nola1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1930948 Gar1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002085531 – 231H/ACA ribonucleoprotein complex subunit 1Add BLAST231

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki145Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9CY66
PRIDEiQ9CY66

PTM databases

iPTMnetiQ9CY66
PhosphoSitePlusiQ9CY66

Expressioni

Gene expression databases

BgeeiENSMUSG00000028010
ExpressionAtlasiQ9CY66 baseline and differential
GenevisibleiQ9CY66 MM

Interactioni

Subunit structurei

Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which contains NHP2/NOLA2, GAR1/NOLA1, NOP10/NOLA3, and DKC1/NOLA4, which is presumed to be the catalytic subunit. The complex contains a stable core formed by binding of one or two NOP10-DKC1 heterodimers to NHP2; GAR1 subsequently binds to this core via DKC1. The complex binds a box H/ACA small nucleolar RNA (snoRNA), which may target the specific site of modification within the RNA substrate. The complex also interacts with TERC, which contains a 3'-terminal domain related to the box H/ACA snoRNAs. Specific interactions with snoRNAs or TERC are mediated by GAR1 and NHP2. Associates with NOLC1/NOPP140. H/ACA snoRNPs interact with the SMN complex, consisting of SMN1 or SMN2, GEMIN2/SIP1, DDX20/GEMIN3, and GEMIN4. This is mediated by interaction between GAR1 and SMN1 or SMN2. The SMN complex may be required for correct assembly of the H/ACA snoRNP complex. Component of the telomerase holoenzyme complex at least composed of TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and a telomerase RNA template component (TERC) (By similarity).By similarity

Protein-protein interaction databases

BioGridi212682, 3 interactors
IntActiQ9CY66, 4 interactors
MINTiQ9CY66
STRINGi10090.ENSMUSP00000029643

Structurei

3D structure databases

ProteinModelPortaliQ9CY66
SMRiQ9CY66
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni4 – 68RGG-box 1Add BLAST65
Regioni180 – 231RGG-box 2Add BLAST52

Domaini

Interaction with SMN1 requires at least one of the RGG-box regions.By similarity

Sequence similaritiesi

Belongs to the GAR1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3262 Eukaryota
COG3277 LUCA
GeneTreeiENSGT00730000111223
InParanoidiQ9CY66
KOiK11128
OMAiKCITHEN
OrthoDBiEOG091G0Z6Z
TreeFamiTF350747

Family and domain databases

Gene3Di2.40.10.230, 1 hit
InterProiView protein in InterPro
IPR038664 Gar1/Naf1_Cbf5-bd_sf
IPR021154 H/ACA_rnp_Gar1
IPR007504 H/ACA_rnp_Gar1/Naf1
IPR009000 Transl_B-barrel_sf
PANTHERiPTHR23237 PTHR23237, 2 hits
PfamiView protein in Pfam
PF04410 Gar1, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD020235 Gar1_RNA_bd, 1 hit
SUPFAMiSSF50447 SSF50447, 1 hit

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CY66-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSFRGGGRGG FNRGGGGGGF NRGGGSNNHF RGGGGGGGGS FRGGGGGGGG
60 70 80 90 100
SFRGGGRGGF GRGGGRGGFN KFQDQGPPER VVLLGEFMHP CEDDIVCKCT
110 120 130 140 150
TEENKVPYFN APVYLENKEQ VGKVDEIFGQ LRDFYFSVKL SENMKASSFK
160 170 180 190 200
KLQKFYIDPY KLLPLQRFLP RPPGEKGPPR GGGGGGRGGR GGGRGGGGRG
210 220 230
GGRGGGFRGG RGGGGGFRGG RGGGGFRGRG H
Length:231
Mass (Da):23,474
Last modified:June 1, 2001 - v1
Checksum:iFDD03BBEE258D662
GO
Isoform 2 (identifier: Q9CY66-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     49-50: Missing.

Show »
Length:229
Mass (Da):23,360
Checksum:i50C18372B6CA72E8
GO
Isoform 3 (identifier: Q9CY66-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     26-37: Missing.
     49-50: Missing.

Show »
Length:217
Mass (Da):22,262
Checksum:i791A30206340BFBF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti52F → L in BAC36056 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01142626 – 37Missing in isoform 3. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_01142749 – 50Missing in isoform 2 and isoform 3. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK021256 mRNA Translation: BAB32351.1
AK075923 mRNA Translation: BAC36056.1
BC021873 mRNA Translation: AAH21873.1
BC048685 mRNA Translation: AAH48685.1
CCDSiCCDS38634.1 [Q9CY66-1]
RefSeqiNP_080854.1, NM_026578.3 [Q9CY66-1]
XP_011238510.1, XM_011240208.2 [Q9CY66-1]
UniGeneiMm.24873

Genome annotation databases

EnsembliENSMUST00000029643; ENSMUSP00000029643; ENSMUSG00000028010 [Q9CY66-1]
GeneIDi68147
KEGGimmu:68147
UCSCiuc008rim.2 mouse [Q9CY66-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGAR1_MOUSE
AccessioniPrimary (citable) accession number: Q9CY66
Secondary accession number(s): Q80X93, Q8C6C5, Q8VDH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: June 1, 2001
Last modified: April 25, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health