Q9CY58 (PAIRB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Plasminogen activator inhibitor 1 RNA-binding protein

Alternative name(s):
PAI1 RNA-binding protein 1
Short name=PAI-RBP1
SERPINE1 mRNA-binding protein 1

Gene names

Name:	Serbp1
Synonyms:	Pairbp1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the regulation of mRNA stability. Binds to the 3'-most 134 nt of the SERPINE1/PAI1 mRNA, a region which confers cyclic nucleotide regulation of message decay By similarity.
Subunit structure	Interacts with CHD3 and TDRD3 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Cytoplasm › perinuclear region By similarity.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Coding sequence diversity	Alternative splicing
Ligand	RNA-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell perinuclear region of cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: Compara
Molecular_function	mRNA 3'-UTR binding Inferred from sequence or structural similarity. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9CY58-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9CY58-2)

The sequence of this isoform differs from the canonical sequence as follows:
     233-247: Missing.

Isoform 3 (identifier: Q9CY58-3)

The sequence of this isoform differs from the canonical sequence as follows:
     203-208: Missing.
     233-247: Missing.

Isoform 4 (identifier: Q9CY58-4)

The sequence of this isoform differs from the canonical sequence as follows:
     233-235: ESP → LVF
     236-407: Missing.

Note: May be due to intron retention. No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 407	406	Plasminogen activator inhibitor 1 RNA-binding protein		PRO_0000058183
Amino acid modifications
	Modified residue	25	1	Phosphoserine Ref.3 Ref.4 Ref.6
	Modified residue	68	1	N6-acetyllysine By similarity
	Modified residue	122	1	N6-acetyllysine By similarity
	Modified residue	140	1	N6-acetyllysine By similarity
	Modified residue	211	1	N6-acetyllysine By similarity
	Modified residue	234	1	Phosphoserine Ref.5
	Modified residue	244	1	Phosphotyrosine Ref.5
	Modified residue	252	1	Phosphoserine Ref.5
	Modified residue	329	1	Phosphoserine Ref.7
	Modified residue	390	1	Phosphoserine Ref.3
	Modified residue	391	1	Phosphoserine By similarity
	Modified residue	393	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	203 – 208	6	Missing in isoform 3.		VSP_011632
	Alternative sequence	233 – 247	15	Missing in isoform 2 and isoform 3.		VSP_011633
	Alternative sequence	233 – 235	3	ESP → LVF in isoform 4.		VSP_011634
	Alternative sequence	236 – 407	172	Missing in isoform 4.		VSP_011635
Experimental info
	Sequence conflict	5	1	L → P in BAC40253. Ref.1
	Sequence conflict	403	1	F → S in BAB23466. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: AC4F61EF7F38A8E5	FASTA	407	44,714
	10 20 30 40 50 60 MPGHLQEGFG CVVTNRFDQL FDDESDPFEV LKAAENKKKE AGGGGVGGPG AKSAAQAAAQ 70 80 90 100 110 120 TNSNAAGKQL RKESQKDRKN PLPPSVGVAD KKEETQPPVA LKKEGIRRVG RRPDQQLQGD 130 140 150 160 170 180 GKLIDRRAER RPPRERRFEK PLEEKGEGGE FSVDRPIIER PIRGRGGLGR GRGGRGRGMG 190 200 210 220 230 240 RGDGFDSRGK REFDRHSGSD RSSFSHYSGL KHEDKRGGSG SHNWGTVKDE LTESPKYIQK 250 260 270 280 290 300 QISYNCSDLD QSNVTEETPE GEEHPVADTE NKENEVEEVK EEGPKEMTLD EWKAIQNKDR 310 320 330 340 350 360 AKVEFNIRKP NEGADGQWKK GFVLHKSKSE EAHAEDSVMD HHFRKPANDI TSQLEINFGD 370 380 390 400 LGRPGRGGRG GRGGRGRGGR PNRGSRTDKS SASAPDVDDP EAFPALA
	Isoform 2 [UniParc]. Checksum: 1569ACE4336B118B	FASTA	392	42,944
	10 20 30 40 50 60 MPGHLQEGFG CVVTNRFDQL FDDESDPFEV LKAAENKKKE AGGGGVGGPG AKSAAQAAAQ 70 80 90 100 110 120 TNSNAAGKQL RKESQKDRKN PLPPSVGVAD KKEETQPPVA LKKEGIRRVG RRPDQQLQGD 130 140 150 160 170 180 GKLIDRRAER RPPRERRFEK PLEEKGEGGE FSVDRPIIER PIRGRGGLGR GRGGRGRGMG 190 200 210 220 230 240 RGDGFDSRGK REFDRHSGSD RSSFSHYSGL KHEDKRGGSG SHNWGTVKDE LTDLDQSNVT 250 260 270 280 290 300 EETPEGEEHP VADTENKENE VEEVKEEGPK EMTLDEWKAI QNKDRAKVEF NIRKPNEGAD 310 320 330 340 350 360 GQWKKGFVLH KSKSEEAHAE DSVMDHHFRK PANDITSQLE INFGDLGRPG RGGRGGRGGR 370 380 390 GRGGRPNRGS RTDKSSASAP DVDDPEAFPA LA
	Isoform 3 [UniParc]. Checksum: A22FB50BE68F72CB	FASTA	386	42,235
	10 20 30 40 50 60 MPGHLQEGFG CVVTNRFDQL FDDESDPFEV LKAAENKKKE AGGGGVGGPG AKSAAQAAAQ 70 80 90 100 110 120 TNSNAAGKQL RKESQKDRKN PLPPSVGVAD KKEETQPPVA LKKEGIRRVG RRPDQQLQGD 130 140 150 160 170 180 GKLIDRRAER RPPRERRFEK PLEEKGEGGE FSVDRPIIER PIRGRGGLGR GRGGRGRGMG 190 200 210 220 230 240 RGDGFDSRGK REFDRHSGSD RSGLKHEDKR GGSGSHNWGT VKDELTDLDQ SNVTEETPEG 250 260 270 280 290 300 EEHPVADTEN KENEVEEVKE EGPKEMTLDE WKAIQNKDRA KVEFNIRKPN EGADGQWKKG 310 320 330 340 350 360 FVLHKSKSEE AHAEDSVMDH HFRKPANDIT SQLEINFGDL GRPGRGGRGG RGGRGRGGRP 370 380 NRGSRTDKSS ASAPDVDDPE AFPALA
	Isoform 4 [UniParc]. Checksum: 687C4A3292F7B607	FASTA	235	25,679
	10 20 30 40 50 60 MPGHLQEGFG CVVTNRFDQL FDDESDPFEV LKAAENKKKE AGGGGVGGPG AKSAAQAAAQ 70 80 90 100 110 120 TNSNAAGKQL RKESQKDRKN PLPPSVGVAD KKEETQPPVA LKKEGIRRVG RRPDQQLQGD 130 140 150 160 170 180 GKLIDRRAER RPPRERRFEK PLEEKGEGGE FSVDRPIIER PIRGRGGLGR GRGGRGRGMG 190 200 210 220 230 RGDGFDSRGK REFDRHSGSD RSSFSHYSGL KHEDKRGGSG SHNWGTVKDE LTLVF

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2; 3 AND 4). Strain: C57BL/6J and NOD. Tissue: Embryo, Embryonic liver, Lung and Thymus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: FVB/N. Tissue: Eye and Salivary gland.
[3]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25 AND SER-390, MASS SPECTROMETRY. Tissue: Embryonic brain.
[4]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25, MASS SPECTROMETRY. Tissue: Liver.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234; SER-252 AND TYR-244, MASS SPECTROMETRY. Tissue: Liver.
[6]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25, MASS SPECTROMETRY. Tissue: Macrophage.
[7]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-329, MASS SPECTROMETRY. Tissue: Embryonic fibroblast.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK004678 mRNA. Translation: BAB23466.1. AK010860 mRNA. Translation: BAB27229.1. AK012390 mRNA. Translation: BAB28207.1. AK045190 mRNA. Translation: BAC32255.1. AK088278 mRNA. Translation: BAC40253.1. BC013665 mRNA. Translation: AAH13665.1. BC030502 mRNA. Translation: AAH30502.1.
IPI	IPI00471475. IPI00471476. IPI00471477. IPI00471478.
RefSeq	NP_001107037.1. NM_001113565.1. NP_001107038.1. NM_001113566.1. NP_080090.2. NM_025814.2.
UniGene	Mm.240490. Mm.440861.
3D structure databases
ProteinModelPortal	Q9CY58.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9CY58. 1 interaction.
PTM databases
PhosphoSite	Q9CY58.
Proteomic databases
PaxDb	Q9CY58.
PRIDE	Q9CY58.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000042990; ENSMUSP00000039110; ENSMUSG00000036371.
GeneID	66870.
KEGG	mmu:66870.
UCSC	uc009cfh.1. mouse. uc009cfi.1. mouse. uc009cfj.2. mouse.
Organism-specific databases
CTD	26135.
MGI	MGI:1914120. Serbp1.
Phylogenomic databases
eggNOG	NOG321910.
GeneTree	ENSGT00520000055591.
HOGENOM	HOG000220826.
HOVERGEN	HBG056357.
InParanoid	Q9CY58.
KO	K13199.
OMA	PKYIQKP.
OrthoDB	EOG4DZ1W6.
Gene expression databases
ArrayExpress	Q9CY58.
Bgee	Q9CY58.
CleanEx	MM_SERBP1.
Genevestigator	Q9CY58.
GermOnline	ENSMUSG00000036371. Mus musculus.
Family and domain databases
InterPro	IPR006861. HABP4_PAIRBP1-bd. IPR027205. SERBP1. [Graphical view]
PANTHER	PTHR12299:SF8. PTHR12299:SF8. 1 hit.
Pfam	PF04774. HABP4_PAI-RBP1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	SERBP1. mouse.
NextBio	322883.
SOURCE	Search...

Entry information

Entry name

PAIRB_MOUSE

Accession

Primary (citable) accession number: Q9CY58
Secondary accession number(s): Q8BHS2 , Q8BHU0, Q91WP3, Q9CSN0, Q9DBY6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 27, 2004
Last sequence update:	September 27, 2004
Last modified:	April 3, 2013
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program