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Protein

Chromatin target of PRMT1 protein

Gene

Chtop

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in the ligand-dependent activation of estrogen receptor target genes (By similarity). May play a role in the silencing of fetal globin genes (PubMed:20688955). Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed:22872859). Required for the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC methylates H4R3 and transactivates genes involved in glioblastomagenesis (PubMed:25284789).By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72187. mRNA 3'-end processing.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromatin target of PRMT1 protein
Alternative name(s):
Friend of PRMT1 protein
Small arginine- and glycine-rich protein
Short name:
SRAG
Gene namesi
Name:Chtop
Synonyms:Fop
ORF Names:MNCb-1706
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1913761. Chtop.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000892642 – 249Chromatin target of PRMT1 proteinAdd BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei40PhosphoserineBy similarity1
Modified residuei49PhosphoserineBy similarity1
Modified residuei64PhosphoserineBy similarity1
Modified residuei243PhosphothreonineBy similarity1

Post-translational modificationi

Asymmetrically methylated by PRMT1. Symmetrically methylated by PRMT5.1 Publication

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiQ9CY57.
PRIDEiQ9CY57.

PTM databases

iPTMnetiQ9CY57.
PhosphoSitePlusiQ9CY57.

Expressioni

Tissue specificityi

Broadly expressed with highest levels found in thymus, spleen, and lymph nodes. Expressed in an erythroid progenitor cell line derived from fetal liver.2 Publications

Developmental stagei

Broadly expressed at E16.5.1 Publication

Gene expression databases

BgeeiENSMUSG00000001017.
CleanExiMM_2500003M10RIK.
ExpressionAtlasiQ9CY57. baseline and differential.
GenevisibleiQ9CY57. MM.

Interactioni

Subunit structurei

Interacts with PRMT1 and PRMT5 (PubMed:19858291, PubMed:22872859). Interacts with the 5FMC complex; the interaction is methylation-dependent. Interacts with FYTTD1, SET and PRC1 complex members CBX4, RNF2 and PHC2; the interactions are methylation-independent. Interacts with ZNF148 (PubMed:22872859). Interacts with WDR77 and ERH (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Prmt1Q9JIF08EBI-6393116,EBI-519055
Prmt5Q8CIG84EBI-6393116,EBI-2527009

Protein-protein interaction databases

BioGridi211527. 3 interactors.
IntActiQ9CY57. 4 interactors.
MINTiMINT-1867998.
STRINGi10090.ENSMUSP00000001043.

Structurei

3D structure databases

ProteinModelPortaliQ9CY57.
SMRiQ9CY57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni154 – 207Interaction with PRMT1Add BLAST54

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi195 – 204GAR motif; involved in 5hmC bindingBy similarity10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi88 – 209Arg/Gly-richAdd BLAST122

Phylogenomic databases

eggNOGiENOG410IWUS. Eukaryota.
ENOG4111XP9. LUCA.
GeneTreeiENSGT00390000002869.
HOVERGENiHBG058458.
InParanoidiQ9CY57.
OMAiRGRGMVG.
OrthoDBiEOG091G1CHE.
PhylomeDBiQ9CY57.
TreeFamiTF331447.

Family and domain databases

InterProiIPR025715. FoP_C.
[Graphical view]
PfamiPF13865. FoP_duplication. 1 hit.
[Graphical view]
SMARTiSM01218. FoP_duplication. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CY57-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAQSAPKVV LKSTTKMSLN ERFTNMLKNK QPMPVNIRAS MQQQQQLASA
60 70 80 90 100
RNRRLAQQME NRPSVQAALK LKQKSLKQRL GKSNIQARLG RPIGALARGA
110 120 130 140 150
IGGRGLPIIQ RGLPRGGLRG GRATRTLLRG GMSLRGQNLL RGGRAVAPRM
160 170 180 190 200
GLRRGGVRGR GGPGRGGLGR GAMGRGGIGG RGRGMIGRGR GGFGGRGRGR
210 220 230 240
GRGRGALTRP VLTKEQLDNQ LDAYMSKTKG HLDAELDAYM AQTDPETND
Length:249
Mass (Da):26,585
Last modified:March 1, 2003 - v2
Checksum:i5F3E44A53ED0645F
GO
Isoform 2 (identifier: Q9CY57-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.

Note: May be due to an intron retention.
Show »
Length:224
Mass (Da):23,850
Checksum:i6D480418EFCABB24
GO
Isoform 3 (identifier: Q9CY57-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     74-74: Missing.
     137-182: Missing.

Note: No experimental confirmation available.
Show »
Length:202
Mass (Da):21,978
Checksum:i9D045E5C3055D068
GO
Isoform 4 (identifier: Q9CY57-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.
     137-182: Missing.

Note: No experimental confirmation available.
Show »
Length:178
Mass (Da):19,371
Checksum:i02A7E08E03DD3BF2
GO
Isoform 5 (identifier: Q9CY57-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-25: Missing.
     74-74: Missing.
     137-182: Missing.

Note: No experimental confirmation available.
Show »
Length:177
Mass (Da):19,243
Checksum:iB54CBF9F53701E1F
GO

Sequence cautioni

The sequence BAA95102 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB25951 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC37084 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96L → Q in BAE31263 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0117431 – 25Missing in isoform 2, isoform 4 and isoform 5. 3 PublicationsAdd BLAST25
Alternative sequenceiVSP_01174474Missing in isoform 3 and isoform 5. 1 Publication1
Alternative sequenceiVSP_011745137 – 182Missing in isoform 3, isoform 4 and isoform 5. 2 PublicationsAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041654 mRNA. Translation: BAA95102.1. Different initiation.
AK004558 mRNA. Translation: BAB23371.1.
AK005377 mRNA. Translation: BAB23984.2.
AK008885 mRNA. Translation: BAB25951.1. Different initiation.
AK010866 mRNA. Translation: BAB27232.2.
AK047914 mRNA. Translation: BAC33189.1.
AK077960 mRNA. Translation: BAC37084.1. Different initiation.
AK132769 mRNA. Translation: BAE21348.1.
AK149010 mRNA. Translation: BAE28718.1.
AK152492 mRNA. Translation: BAE31263.1.
CH466547 Genomic DNA. Translation: EDL15130.1.
BC083333 mRNA. Translation: AAH83333.1.
BC120651 mRNA. Translation: AAI20652.1.
CCDSiCCDS17532.1. [Q9CY57-2]
CCDS79964.1. [Q9CY57-5]
CCDS79965.1. [Q9CY57-4]
CCDS79966.1. [Q9CY57-3]
CCDS79968.1. [Q9CY57-1]
RefSeqiNP_001280705.1. NM_001293776.1. [Q9CY57-1]
NP_001280706.1. NM_001293777.1.
NP_001280707.1. NM_001293778.1.
NP_001280708.1. NM_001293779.1. [Q9CY57-3]
NP_001280709.1. NM_001293780.1. [Q9CY57-4]
NP_001280710.1. NM_001293781.1. [Q9CY57-5]
NP_001280711.1. NM_001293782.1. [Q9CY57-2]
NP_075704.2. NM_023215.6. [Q9CY57-2]
UniGeneiMm.154136.

Genome annotation databases

EnsembliENSMUST00000001043; ENSMUSP00000001043; ENSMUSG00000001017. [Q9CY57-2]
ENSMUST00000049937; ENSMUSP00000061800; ENSMUSG00000001017. [Q9CY57-1]
ENSMUST00000076639; ENSMUSP00000075936; ENSMUSG00000001017. [Q9CY57-3]
ENSMUST00000107342; ENSMUSP00000102965; ENSMUSG00000001017. [Q9CY57-5]
ENSMUST00000107343; ENSMUSP00000102966; ENSMUSG00000001017. [Q9CY57-4]
ENSMUST00000107344; ENSMUSP00000102967; ENSMUSG00000001017. [Q9CY57-2]
GeneIDi66511.
KEGGimmu:66511.
UCSCiuc008qco.2. mouse. [Q9CY57-1]
uc008qcr.2. mouse. [Q9CY57-3]
uc056ztp.1. mouse. [Q9CY57-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041654 mRNA. Translation: BAA95102.1. Different initiation.
AK004558 mRNA. Translation: BAB23371.1.
AK005377 mRNA. Translation: BAB23984.2.
AK008885 mRNA. Translation: BAB25951.1. Different initiation.
AK010866 mRNA. Translation: BAB27232.2.
AK047914 mRNA. Translation: BAC33189.1.
AK077960 mRNA. Translation: BAC37084.1. Different initiation.
AK132769 mRNA. Translation: BAE21348.1.
AK149010 mRNA. Translation: BAE28718.1.
AK152492 mRNA. Translation: BAE31263.1.
CH466547 Genomic DNA. Translation: EDL15130.1.
BC083333 mRNA. Translation: AAH83333.1.
BC120651 mRNA. Translation: AAI20652.1.
CCDSiCCDS17532.1. [Q9CY57-2]
CCDS79964.1. [Q9CY57-5]
CCDS79965.1. [Q9CY57-4]
CCDS79966.1. [Q9CY57-3]
CCDS79968.1. [Q9CY57-1]
RefSeqiNP_001280705.1. NM_001293776.1. [Q9CY57-1]
NP_001280706.1. NM_001293777.1.
NP_001280707.1. NM_001293778.1.
NP_001280708.1. NM_001293779.1. [Q9CY57-3]
NP_001280709.1. NM_001293780.1. [Q9CY57-4]
NP_001280710.1. NM_001293781.1. [Q9CY57-5]
NP_001280711.1. NM_001293782.1. [Q9CY57-2]
NP_075704.2. NM_023215.6. [Q9CY57-2]
UniGeneiMm.154136.

3D structure databases

ProteinModelPortaliQ9CY57.
SMRiQ9CY57.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211527. 3 interactors.
IntActiQ9CY57. 4 interactors.
MINTiMINT-1867998.
STRINGi10090.ENSMUSP00000001043.

PTM databases

iPTMnetiQ9CY57.
PhosphoSitePlusiQ9CY57.

Proteomic databases

PaxDbiQ9CY57.
PRIDEiQ9CY57.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001043; ENSMUSP00000001043; ENSMUSG00000001017. [Q9CY57-2]
ENSMUST00000049937; ENSMUSP00000061800; ENSMUSG00000001017. [Q9CY57-1]
ENSMUST00000076639; ENSMUSP00000075936; ENSMUSG00000001017. [Q9CY57-3]
ENSMUST00000107342; ENSMUSP00000102965; ENSMUSG00000001017. [Q9CY57-5]
ENSMUST00000107343; ENSMUSP00000102966; ENSMUSG00000001017. [Q9CY57-4]
ENSMUST00000107344; ENSMUSP00000102967; ENSMUSG00000001017. [Q9CY57-2]
GeneIDi66511.
KEGGimmu:66511.
UCSCiuc008qco.2. mouse. [Q9CY57-1]
uc008qcr.2. mouse. [Q9CY57-3]
uc056ztp.1. mouse. [Q9CY57-5]

Organism-specific databases

CTDi26097.
MGIiMGI:1913761. Chtop.

Phylogenomic databases

eggNOGiENOG410IWUS. Eukaryota.
ENOG4111XP9. LUCA.
GeneTreeiENSGT00390000002869.
HOVERGENiHBG058458.
InParanoidiQ9CY57.
OMAiRGRGMVG.
OrthoDBiEOG091G1CHE.
PhylomeDBiQ9CY57.
TreeFamiTF331447.

Enzyme and pathway databases

ReactomeiR-MMU-109688. Cleavage of Growing Transcript in the Termination Region.
R-MMU-159236. Transport of Mature mRNA derived from an Intron-Containing Transcript.
R-MMU-72187. mRNA 3'-end processing.

Miscellaneous databases

PROiQ9CY57.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001017.
CleanExiMM_2500003M10RIK.
ExpressionAtlasiQ9CY57. baseline and differential.
GenevisibleiQ9CY57. MM.

Family and domain databases

InterProiIPR025715. FoP_C.
[Graphical view]
PfamiPF13865. FoP_duplication. 1 hit.
[Graphical view]
SMARTiSM01218. FoP_duplication. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHTOP_MOUSE
AccessioniPrimary (citable) accession number: Q9CY57
Secondary accession number(s): Q0VBG3
, Q3U7V6, Q3UF43, Q3V105, Q5XJG9, Q8C5N4, Q9D7T3, Q9DB03, Q9DC54, Q9JJ95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.