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Protein

Probable tRNA(His) guanylyltransferase

Gene

Thg1l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis (By similarity).By similarity

Catalytic activityi

p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi58 – 581Magnesium 1; catalyticBy similarity
Metal bindingi58 – 581Magnesium 2; catalyticBy similarity
Metal bindingi59 – 591Magnesium 1; via carbonyl oxygen; catalyticBy similarity
Metal bindingi105 – 1051Magnesium 1; catalyticBy similarity
Metal bindingi105 – 1051Magnesium 2; catalyticBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi58 – 636GTPBy similarity
Nucleotide bindingi104 – 1052GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA(His) guanylyltransferase (EC:2.7.7.79)
Alternative name(s):
tRNA-histidine guanylyltransferase
Gene namesi
Name:Thg1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1913878. Thg1l.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Probable tRNA(His) guanylyltransferasePRO_0000284985Add
BLAST

Proteomic databases

EPDiQ9CY52.
MaxQBiQ9CY52.
PaxDbiQ9CY52.
PRIDEiQ9CY52.

PTM databases

iPTMnetiQ9CY52.
PhosphoSiteiQ9CY52.

Expressioni

Gene expression databases

BgeeiQ9CY52.
ExpressionAtlasiQ9CY52. baseline and differential.
GenevisibleiQ9CY52. MM.

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000011398.

Structurei

3D structure databases

ProteinModelPortaliQ9CY52.
SMRiQ9CY52. Positions 33-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2721. Eukaryota.
COG4021. LUCA.
GeneTreeiENSGT00390000011705.
HOGENOMiHOG000204890.
HOVERGENiHBG054289.
InParanoidiQ9CY52.
KOiK10761.
OMAiRFEYVKS.
OrthoDBiEOG7X0VHR.
PhylomeDBiQ9CY52.
TreeFamiTF325119.

Family and domain databases

InterProiIPR025845. Thg1_C_dom.
IPR024956. tRNAHis_GuaTrfase_cat.
IPR007537. tRNAHis_GuaTrfase_Thg1.
[Graphical view]
PANTHERiPTHR12729. PTHR12729. 1 hit.
PfamiPF04446. Thg1. 1 hit.
PF14413. Thg1C. 1 hit.
[Graphical view]
PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CY52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAFRTARIG SLLAATSVIL RRCLRLGVAM AKSKFEYVRN FEVQDTCLPH
60 70 80 90 100
CWVVVRLDGR NFHRFAEEHN FAKPNDSRAL HLMTKCAQTV MEELEDIVIA
110 120 130 140 150
YGQSDEYSFV FRKKSNWFKR RASKFMTLVA SQFASSYVFY WRDYFEDQPL
160 170 180 190 200
RYPPGFDGRV VLYPSNQTLK DYLSWRQADC HINNLYNTVF WALIQQSGLT
210 220 230 240 250
PVQAQQRLKG TLTADKNEIL FSEFHINYNN EPHMYRKGTV LVWQKVEEVR
260 270 280 290
TQEVRLPAEM EGEKKAVART RTRVVALNCD LIGDAFWKEH PEILAEEN
Length:298
Mass (Da):34,971
Last modified:June 1, 2001 - v1
Checksum:iD4CA0C154E452ECA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti192 – 1921A → V in AAH92541 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010876 mRNA. Translation: BAB27240.1.
AL645948 Genomic DNA. Translation: CAI25024.1.
BC092541 mRNA. Translation: AAH92541.1.
BC115561 mRNA. Translation: AAI15562.1.
BC115560 mRNA. Translation: AAI15561.1.
CCDSiCCDS36132.1.
RefSeqiNP_001074438.1. NM_001080969.3.
UniGeneiMm.269083.

Genome annotation databases

EnsembliENSMUST00000011398; ENSMUSP00000011398; ENSMUSG00000011254.
GeneIDi66628.
KEGGimmu:66628.
UCSCiuc007inv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010876 mRNA. Translation: BAB27240.1.
AL645948 Genomic DNA. Translation: CAI25024.1.
BC092541 mRNA. Translation: AAH92541.1.
BC115561 mRNA. Translation: AAI15562.1.
BC115560 mRNA. Translation: AAI15561.1.
CCDSiCCDS36132.1.
RefSeqiNP_001074438.1. NM_001080969.3.
UniGeneiMm.269083.

3D structure databases

ProteinModelPortaliQ9CY52.
SMRiQ9CY52. Positions 33-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000011398.

PTM databases

iPTMnetiQ9CY52.
PhosphoSiteiQ9CY52.

Proteomic databases

EPDiQ9CY52.
MaxQBiQ9CY52.
PaxDbiQ9CY52.
PRIDEiQ9CY52.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000011398; ENSMUSP00000011398; ENSMUSG00000011254.
GeneIDi66628.
KEGGimmu:66628.
UCSCiuc007inv.2. mouse.

Organism-specific databases

CTDi54974.
MGIiMGI:1913878. Thg1l.

Phylogenomic databases

eggNOGiKOG2721. Eukaryota.
COG4021. LUCA.
GeneTreeiENSGT00390000011705.
HOGENOMiHOG000204890.
HOVERGENiHBG054289.
InParanoidiQ9CY52.
KOiK10761.
OMAiRFEYVKS.
OrthoDBiEOG7X0VHR.
PhylomeDBiQ9CY52.
TreeFamiTF325119.

Miscellaneous databases

PROiQ9CY52.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CY52.
ExpressionAtlasiQ9CY52. baseline and differential.
GenevisibleiQ9CY52. MM.

Family and domain databases

InterProiIPR025845. Thg1_C_dom.
IPR024956. tRNAHis_GuaTrfase_cat.
IPR007537. tRNAHis_GuaTrfase_Thg1.
[Graphical view]
PANTHERiPTHR12729. PTHR12729. 1 hit.
PfamiPF04446. Thg1. 1 hit.
PF14413. Thg1C. 1 hit.
[Graphical view]
PIRSFiPIRSF028980. tRNAHis_guanylyltransferase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Spleen and Testis.

Entry informationi

Entry nameiTHG1_MOUSE
AccessioniPrimary (citable) accession number: Q9CY52
Secondary accession number(s): Q562D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2001
Last modified: June 8, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.