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Protein

Probable tRNA(His) guanylyltransferase

Gene

Thg1l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adds a GMP to the 5'-end of tRNA(His) after transcription and RNase P cleavage. This step is essential for proper recognition of the tRNA and for the fidelity of protein synthesis (By similarity).By similarity

Catalytic activityi

p-tRNA(His) + ATP + GTP = pppG-P-tRNA(His) + AMP + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi58Magnesium 1; catalyticBy similarity1
Metal bindingi58Magnesium 2; catalyticBy similarity1
Metal bindingi59Magnesium 1; via carbonyl oxygen; catalyticBy similarity1
Metal bindingi105Magnesium 1; catalyticBy similarity1
Metal bindingi105Magnesium 2; catalyticBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi58 – 63GTPBy similarity6
Nucleotide bindingi104 – 105GTPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processtRNA processing
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA(His) guanylyltransferase (EC:2.7.7.79)
Alternative name(s):
tRNA-histidine guanylyltransferase
Gene namesi
Name:Thg1l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1913878 Thg1l

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002849851 – 298Probable tRNA(His) guanylyltransferaseAdd BLAST298

Proteomic databases

EPDiQ9CY52
MaxQBiQ9CY52
PaxDbiQ9CY52
PeptideAtlasiQ9CY52
PRIDEiQ9CY52

PTM databases

iPTMnetiQ9CY52
PhosphoSitePlusiQ9CY52

Expressioni

Gene expression databases

BgeeiENSMUSG00000011254
ExpressionAtlasiQ9CY52 baseline and differential
GenevisibleiQ9CY52 MM

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000011398

Structurei

3D structure databases

ProteinModelPortaliQ9CY52
SMRiQ9CY52
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2721 Eukaryota
COG4021 LUCA
GeneTreeiENSGT00390000011705
HOGENOMiHOG000204890
HOVERGENiHBG054289
InParanoidiQ9CY52
KOiK10761
OMAiNTAFWKL
OrthoDBiEOG091G0GYS
PhylomeDBiQ9CY52
TreeFamiTF325119

Family and domain databases

Gene3Di3.30.70.3000, 1 hit
InterProiView protein in InterPro
IPR025845 Thg1_C_dom
IPR024956 tRNAHis_GuaTrfase_cat
IPR007537 tRNAHis_GuaTrfase_Thg1
IPR038469 tRNAHis_GuaTrfase_Thg1_sf
PANTHERiPTHR12729 PTHR12729, 1 hit
PfamiView protein in Pfam
PF04446 Thg1, 1 hit
PF14413 Thg1C, 1 hit
PIRSFiPIRSF028980 tRNAHis_guanylyltransferase, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CY52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWAFRTARIG SLLAATSVIL RRCLRLGVAM AKSKFEYVRN FEVQDTCLPH
60 70 80 90 100
CWVVVRLDGR NFHRFAEEHN FAKPNDSRAL HLMTKCAQTV MEELEDIVIA
110 120 130 140 150
YGQSDEYSFV FRKKSNWFKR RASKFMTLVA SQFASSYVFY WRDYFEDQPL
160 170 180 190 200
RYPPGFDGRV VLYPSNQTLK DYLSWRQADC HINNLYNTVF WALIQQSGLT
210 220 230 240 250
PVQAQQRLKG TLTADKNEIL FSEFHINYNN EPHMYRKGTV LVWQKVEEVR
260 270 280 290
TQEVRLPAEM EGEKKAVART RTRVVALNCD LIGDAFWKEH PEILAEEN
Length:298
Mass (Da):34,971
Last modified:June 1, 2001 - v1
Checksum:iD4CA0C154E452ECA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192A → V in AAH92541 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010876 mRNA Translation: BAB27240.1
AL645948 Genomic DNA No translation available.
BC092541 mRNA Translation: AAH92541.1
BC115561 mRNA Translation: AAI15562.1
BC115560 mRNA Translation: AAI15561.1
CCDSiCCDS36132.1
RefSeqiNP_001074438.1, NM_001080969.3
UniGeneiMm.269083

Genome annotation databases

EnsembliENSMUST00000011398; ENSMUSP00000011398; ENSMUSG00000011254
GeneIDi66628
KEGGimmu:66628
UCSCiuc007inv.2 mouse

Similar proteinsi

Entry informationi

Entry nameiTHG1_MOUSE
AccessioniPrimary (citable) accession number: Q9CY52
Secondary accession number(s): Q562D7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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