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Protein

NEDD8-conjugating enzyme UBE2F

Gene

Ube2f

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.1 Publication

Catalytic activityi

S-NEDD8yl-[E2 NEDD8-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 NEDD8-conjugating enzyme]-L-cysteine + N(6)-NEDD8yl-[acceptor protein]-L-lysine.

Pathwayi: protein neddylation

This protein is involved in the pathway protein neddylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein neddylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei116Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • NEDD8 transferase activity Source: UniProtKB
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin protein ligase binding Source: GO_Central

GO - Biological processi

  • protein neddylation Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00885.

Names & Taxonomyi

Protein namesi
Recommended name:
NEDD8-conjugating enzyme UBE2F (EC:2.3.2.-)
Alternative name(s):
NEDD8 carrier protein UBE2F
NEDD8 protein ligase UBE2F
NEDD8-conjugating enzyme 2
RING-type E3 NEDD8 transferase UBE2F
Ubiquitin-conjugating enzyme E2 F
Gene namesi
Name:Ube2f
Synonyms:Ube2fa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1915171. Ube2f.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002630781 – 185NEDD8-conjugating enzyme UBE2FAdd BLAST185

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Post-translational modificationi

The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9CY34.
MaxQBiQ9CY34.
PaxDbiQ9CY34.
PeptideAtlasiQ9CY34.
PRIDEiQ9CY34.

PTM databases

iPTMnetiQ9CY34.
PhosphoSitePlusiQ9CY34.

Expressioni

Tissue specificityi

Widely expressed (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000034343.
CleanExiMM_UBE2F.
ExpressionAtlasiQ9CY34. baseline and differential.
GenevisibleiQ9CY34. MM.

Interactioni

Subunit structurei

Interacts with UBA3 and RBX2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054303.

Structurei

3D structure databases

ProteinModelPortaliQ9CY34.
SMRiQ9CY34.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 29Interaction with UBA3By similarityAdd BLAST29

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0420. Eukaryota.
ENOG410XS81. LUCA.
GeneTreeiENSGT00630000089859.
HOGENOMiHOG000233456.
InParanoidiQ9CY34.
KOiK10687.
OMAiLHHFQLA.
OrthoDBiEOG091G0N8A.
PhylomeDBiQ9CY34.
TreeFamiTF101125.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CY34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTLASKLKR DDGLKGSRTS ASTSDSTRRV SVRDKLLVKE VAELEANLPC
60 70 80 90 100
TCKVHFPDPN KLHCFQLTVS PDEGYYQGGK FQFETEVPDA YNMVPPKVKC
110 120 130 140 150
LTKIWHPNIT ETGEICLSLL REHSIDGTGW APTRTLKDVV WGLNSLFTDL
160 170 180
LNFDDPLNIE AAEHHLRDKE DFRDKVDEYI KRYAR
Length:185
Mass (Da):21,110
Last modified:June 1, 2001 - v1
Checksum:iDE16464086DBFB06
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30V → C in BAB25362 (PubMed:16141072).Curated1
Sequence conflicti31S → A in BAB25372 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007937 mRNA. Translation: BAB25362.1.
AK007960 mRNA. Translation: BAB25372.1.
AK010957 mRNA. Translation: BAB27290.1.
AK030594 mRNA. Translation: BAC27036.1.
AK153481 mRNA. Translation: BAE32030.1.
BC006057 mRNA. Translation: AAH06057.1.
BC016117 mRNA. Translation: AAH16117.1.
CCDSiCCDS48322.1.
RefSeqiNP_080730.1. NM_026454.3.
XP_006529899.1. XM_006529836.3.
XP_006529900.1. XM_006529837.3.
XP_006529901.1. XM_006529838.3.
XP_006529902.1. XM_006529839.3.
XP_006529903.1. XM_006529840.3.
XP_011246369.1. XM_011248067.2.
UniGeneiMm.337238.

Genome annotation databases

EnsembliENSMUST00000059743; ENSMUSP00000054303; ENSMUSG00000034343.
ENSMUST00000171112; ENSMUSP00000131330; ENSMUSG00000034343.
ENSMUST00000171165; ENSMUSP00000126173; ENSMUSG00000034343.
ENSMUST00000178627; ENSMUSP00000136915; ENSMUSG00000034343.
ENSMUST00000191368; ENSMUSP00000140743; ENSMUSG00000034343.
GeneIDi67921.
KEGGimmu:67921.
UCSCiuc007bzy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007937 mRNA. Translation: BAB25362.1.
AK007960 mRNA. Translation: BAB25372.1.
AK010957 mRNA. Translation: BAB27290.1.
AK030594 mRNA. Translation: BAC27036.1.
AK153481 mRNA. Translation: BAE32030.1.
BC006057 mRNA. Translation: AAH06057.1.
BC016117 mRNA. Translation: AAH16117.1.
CCDSiCCDS48322.1.
RefSeqiNP_080730.1. NM_026454.3.
XP_006529899.1. XM_006529836.3.
XP_006529900.1. XM_006529837.3.
XP_006529901.1. XM_006529838.3.
XP_006529902.1. XM_006529839.3.
XP_006529903.1. XM_006529840.3.
XP_011246369.1. XM_011248067.2.
UniGeneiMm.337238.

3D structure databases

ProteinModelPortaliQ9CY34.
SMRiQ9CY34.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000054303.

PTM databases

iPTMnetiQ9CY34.
PhosphoSitePlusiQ9CY34.

Proteomic databases

EPDiQ9CY34.
MaxQBiQ9CY34.
PaxDbiQ9CY34.
PeptideAtlasiQ9CY34.
PRIDEiQ9CY34.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059743; ENSMUSP00000054303; ENSMUSG00000034343.
ENSMUST00000171112; ENSMUSP00000131330; ENSMUSG00000034343.
ENSMUST00000171165; ENSMUSP00000126173; ENSMUSG00000034343.
ENSMUST00000178627; ENSMUSP00000136915; ENSMUSG00000034343.
ENSMUST00000191368; ENSMUSP00000140743; ENSMUSG00000034343.
GeneIDi67921.
KEGGimmu:67921.
UCSCiuc007bzy.1. mouse.

Organism-specific databases

CTDi140739.
MGIiMGI:1915171. Ube2f.

Phylogenomic databases

eggNOGiKOG0420. Eukaryota.
ENOG410XS81. LUCA.
GeneTreeiENSGT00630000089859.
HOGENOMiHOG000233456.
InParanoidiQ9CY34.
KOiK10687.
OMAiLHHFQLA.
OrthoDBiEOG091G0N8A.
PhylomeDBiQ9CY34.
TreeFamiTF101125.

Enzyme and pathway databases

UniPathwayiUPA00885.

Miscellaneous databases

PROiPR:Q9CY34.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034343.
CleanExiMM_UBE2F.
ExpressionAtlasiQ9CY34. baseline and differential.
GenevisibleiQ9CY34. MM.

Family and domain databases

CDDicd00195. UBCc. 1 hit.
Gene3Di3.10.110.10. 1 hit.
InterProiView protein in InterPro
IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
PfamiView protein in Pfam
PF00179. UQ_con. 1 hit.
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiView protein in PROSITE
PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUB2FA_MOUSE
AccessioniPrimary (citable) accession number: Q9CY34
Secondary accession number(s): Q99JK3, Q9D8K0, Q9D8K7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: June 1, 2001
Last modified: May 10, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.