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Q9CXY9

- GPI8_MOUSE

UniProt

Q9CXY9 - GPI8_MOUSE

Protein

GPI-anchor transamidase

Gene

Pigk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 2 (16 Aug 2004)
      Previous versions | rss
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    Functioni

    Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei164 – 1641By similarity
    Active sitei206 – 2061By similarity

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: InterPro
    2. GPI-anchor transamidase activity Source: UniProtKB

    GO - Biological processi

    1. attachment of GPI anchor to protein Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    GPI-anchor biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00196.

    Protein family/group databases

    MEROPSiC13.005.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GPI-anchor transamidase (EC:3.-.-.-)
    Short name:
    GPI transamidase
    Alternative name(s):
    Phosphatidylinositol-glycan biosynthesis class K protein
    Short name:
    PIG-K
    Gene namesi
    Name:Pigk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1913863. Pigk.

    Subcellular locationi

    GO - Cellular componenti

    1. GPI-anchor transamidase complex Source: UniProtKB

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727By similarityAdd
    BLAST
    Chaini28 – 395368GPI-anchor transamidasePRO_0000026530Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi92 – 92Interchain (with C-186 in PIGT)By similarity

    Post-translational modificationi

    The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity.By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiQ9CXY9.
    PaxDbiQ9CXY9.
    PRIDEiQ9CXY9.

    PTM databases

    PhosphoSiteiQ9CXY9.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9CXY9.
    BgeeiQ9CXY9.
    GenevestigatoriQ9CXY9.

    Interactioni

    Subunit structurei

    Forms a complex with PIGT, PIGS, PIGU and GAA1.By similarity

    Protein-protein interaction databases

    IntActiQ9CXY9. 1 interaction.
    STRINGi10090.ENSMUSP00000045351.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CXY9.
    SMRiQ9CXY9. Positions 43-308.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 367340LumenalSequence AnalysisAdd
    BLAST
    Topological domaini389 – 3957CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei368 – 38821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni311 – 32111Essential for GPI attachmentBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C13 family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5206.
    GeneTreeiENSGT00530000063391.
    HOGENOMiHOG000204398.
    HOVERGENiHBG027488.
    KOiK05290.
    OrthoDBiEOG70W3DZ.

    Family and domain databases

    InterProiIPR028361. GPI_transamidase.
    IPR001096. Peptidase_C13.
    [Graphical view]
    PANTHERiPTHR12000. PTHR12000. 1 hit.
    PfamiPF01650. Peptidase_C13. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500138. GPI8. 1 hit.
    PIRSF019663. Legumain. 1 hit.
    PRINTSiPR00776. HEMOGLOBNASE.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9CXY9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAPCFLTLR VATLAALALL SLGSSAAGHI EDQAEQFFRS GHTNNWAVLV    50
    CTSRFWFNYR HVANTLSVYR SVKRLGIPDS HIVLMLADDM ACNARNPKPA 100
    TVFSHKNMEL NVYGDDVEVD YRSYEVTVEN FLRVLTGRVP PSTPRSKRLL 150
    SDDRSNILIY MTGHGGNGFL KFQDSEEITN IELADAFEQM WQKRRYNELL 200
    FIIDTCQGAS MYERFYSPNI MALASSQVGE DSLSHQPDPA IGVHLMDRYT 250
    FYVLEFLEEI NPASQTNMND LFQVCPKSLC VSTPGHRTDL FQRDPKNVLI 300
    TDFFGSVRKV EITTEKISLQ WDSQVVDSSS KEDGTAEERM GPLKYAEQLP 350
    VAQIIHQKPK PRDWHPPGGF ILGLWALIIM VFFKTYGIKH MKFIF 395
    Length:395
    Mass (Da):44,895
    Last modified:August 16, 2004 - v2
    Checksum:i4765ADDF4D46E386
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti125 – 1262EV → RG in BAB29018. (PubMed:16141072)Curated
    Sequence conflicti138 – 1381R → E in BAB29018. (PubMed:16141072)Curated
    Sequence conflicti154 – 1541R → K in BAB29018. (PubMed:16141072)Curated
    Sequence conflicti257 – 2582LE → WK in BAB29018. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK013853 mRNA. Translation: BAB29018.1.
    AK077893 mRNA. Translation: BAC37051.1.
    AK082806 mRNA. Translation: BAC38629.1.
    BC060175 mRNA. Translation: AAH60175.1.
    CCDSiCCDS17919.1.
    RefSeqiNP_079938.1. NM_025662.5.
    NP_821135.1. NM_178016.3.
    UniGeneiMm.331447.

    Genome annotation databases

    EnsembliENSMUST00000159899; ENSMUSP00000123772; ENSMUSG00000039047.
    GeneIDi329777.
    KEGGimmu:329777.
    UCSCiuc012czo.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK013853 mRNA. Translation: BAB29018.1 .
    AK077893 mRNA. Translation: BAC37051.1 .
    AK082806 mRNA. Translation: BAC38629.1 .
    BC060175 mRNA. Translation: AAH60175.1 .
    CCDSi CCDS17919.1.
    RefSeqi NP_079938.1. NM_025662.5.
    NP_821135.1. NM_178016.3.
    UniGenei Mm.331447.

    3D structure databases

    ProteinModelPortali Q9CXY9.
    SMRi Q9CXY9. Positions 43-308.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9CXY9. 1 interaction.
    STRINGi 10090.ENSMUSP00000045351.

    Protein family/group databases

    MEROPSi C13.005.

    PTM databases

    PhosphoSitei Q9CXY9.

    Proteomic databases

    MaxQBi Q9CXY9.
    PaxDbi Q9CXY9.
    PRIDEi Q9CXY9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000159899 ; ENSMUSP00000123772 ; ENSMUSG00000039047 .
    GeneIDi 329777.
    KEGGi mmu:329777.
    UCSCi uc012czo.1. mouse.

    Organism-specific databases

    CTDi 10026.
    MGIi MGI:1913863. Pigk.

    Phylogenomic databases

    eggNOGi COG5206.
    GeneTreei ENSGT00530000063391.
    HOGENOMi HOG000204398.
    HOVERGENi HBG027488.
    KOi K05290.
    OrthoDBi EOG70W3DZ.

    Enzyme and pathway databases

    UniPathwayi UPA00196 .

    Miscellaneous databases

    NextBioi 398962.
    PROi Q9CXY9.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9CXY9.
    Bgeei Q9CXY9.
    Genevestigatori Q9CXY9.

    Family and domain databases

    InterProi IPR028361. GPI_transamidase.
    IPR001096. Peptidase_C13.
    [Graphical view ]
    PANTHERi PTHR12000. PTHR12000. 1 hit.
    Pfami PF01650. Peptidase_C13. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF500138. GPI8. 1 hit.
    PIRSF019663. Legumain. 1 hit.
    PRINTSi PR00776. HEMOGLOBNASE.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryonic head and Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiGPI8_MOUSE
    AccessioniPrimary (citable) accession number: Q9CXY9
    Secondary accession number(s): Q8BH63
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2002
    Last sequence update: August 16, 2004
    Last modified: October 1, 2014
    This is version 95 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3