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Q9CXY9

- GPI8_MOUSE

UniProt

Q9CXY9 - GPI8_MOUSE

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Protein
GPI-anchor transamidase
Gene
Pigk
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Mediates GPI anchoring in the endoplasmic reticulum, by replacing a protein's C-terminal GPI attachment signal peptide with a pre-assembled GPI. During this transamidation reaction, the GPI transamidase forms a carbonyl intermediate with the substrate protein By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641 By similarity
Active sitei206 – 2061 By similarity

GO - Molecular functioni

  1. GPI-anchor transamidase activity Source: UniProtKB
  2. cysteine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. attachment of GPI anchor to protein Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

GPI-anchor biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00196.

Protein family/group databases

MEROPSiC13.005.

Names & Taxonomyi

Protein namesi
Recommended name:
GPI-anchor transamidase (EC:3.-.-.-)
Short name:
GPI transamidase
Alternative name(s):
Phosphatidylinositol-glycan biosynthesis class K protein
Short name:
PIG-K
Gene namesi
Name:Pigk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:1913863. Pigk.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 367340Lumenal Reviewed prediction
Add
BLAST
Transmembranei368 – 38821Helical; Reviewed prediction
Add
BLAST
Topological domaini389 – 3957Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. GPI-anchor transamidase complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 By similarity
Add
BLAST
Chaini28 – 395368GPI-anchor transamidase
PRO_0000026530Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi92 – 92Interchain (with C-186 in PIGT) By similarity

Post-translational modificationi

The disulfide bond between PIGK/GPI8 and PIGT is important for normal enzyme activity By similarity.

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ9CXY9.
PaxDbiQ9CXY9.
PRIDEiQ9CXY9.

PTM databases

PhosphoSiteiQ9CXY9.

Expressioni

Gene expression databases

ArrayExpressiQ9CXY9.
BgeeiQ9CXY9.
GenevestigatoriQ9CXY9.

Interactioni

Subunit structurei

Forms a complex with PIGT, PIGS, PIGU and GAA1 By similarity.

Protein-protein interaction databases

IntActiQ9CXY9. 1 interaction.
STRINGi10090.ENSMUSP00000045351.

Structurei

3D structure databases

ProteinModelPortaliQ9CXY9.
SMRiQ9CXY9. Positions 43-308.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni311 – 32111Essential for GPI attachment By similarity
Add
BLAST

Sequence similaritiesi

Belongs to the peptidase C13 family.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5206.
GeneTreeiENSGT00530000063391.
HOGENOMiHOG000204398.
HOVERGENiHBG027488.
KOiK05290.
OrthoDBiEOG70W3DZ.

Family and domain databases

InterProiIPR028361. GPI_transamidase.
IPR001096. Peptidase_C13.
[Graphical view]
PANTHERiPTHR12000. PTHR12000. 1 hit.
PfamiPF01650. Peptidase_C13. 1 hit.
[Graphical view]
PIRSFiPIRSF500138. GPI8. 1 hit.
PIRSF019663. Legumain. 1 hit.
PRINTSiPR00776. HEMOGLOBNASE.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CXY9-1 [UniParc]FASTAAdd to Basket

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MAAPCFLTLR VATLAALALL SLGSSAAGHI EDQAEQFFRS GHTNNWAVLV    50
CTSRFWFNYR HVANTLSVYR SVKRLGIPDS HIVLMLADDM ACNARNPKPA 100
TVFSHKNMEL NVYGDDVEVD YRSYEVTVEN FLRVLTGRVP PSTPRSKRLL 150
SDDRSNILIY MTGHGGNGFL KFQDSEEITN IELADAFEQM WQKRRYNELL 200
FIIDTCQGAS MYERFYSPNI MALASSQVGE DSLSHQPDPA IGVHLMDRYT 250
FYVLEFLEEI NPASQTNMND LFQVCPKSLC VSTPGHRTDL FQRDPKNVLI 300
TDFFGSVRKV EITTEKISLQ WDSQVVDSSS KEDGTAEERM GPLKYAEQLP 350
VAQIIHQKPK PRDWHPPGGF ILGLWALIIM VFFKTYGIKH MKFIF 395
Length:395
Mass (Da):44,895
Last modified:August 16, 2004 - v2
Checksum:i4765ADDF4D46E386
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti125 – 1262EV → RG in BAB29018. 1 Publication
Sequence conflicti138 – 1381R → E in BAB29018. 1 Publication
Sequence conflicti154 – 1541R → K in BAB29018. 1 Publication
Sequence conflicti257 – 2582LE → WK in BAB29018. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK013853 mRNA. Translation: BAB29018.1.
AK077893 mRNA. Translation: BAC37051.1.
AK082806 mRNA. Translation: BAC38629.1.
BC060175 mRNA. Translation: AAH60175.1.
CCDSiCCDS17919.1.
RefSeqiNP_079938.1. NM_025662.5.
NP_821135.1. NM_178016.3.
UniGeneiMm.331447.

Genome annotation databases

EnsembliENSMUST00000159899; ENSMUSP00000123772; ENSMUSG00000039047.
GeneIDi329777.
KEGGimmu:329777.
UCSCiuc012czo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK013853 mRNA. Translation: BAB29018.1 .
AK077893 mRNA. Translation: BAC37051.1 .
AK082806 mRNA. Translation: BAC38629.1 .
BC060175 mRNA. Translation: AAH60175.1 .
CCDSi CCDS17919.1.
RefSeqi NP_079938.1. NM_025662.5.
NP_821135.1. NM_178016.3.
UniGenei Mm.331447.

3D structure databases

ProteinModelPortali Q9CXY9.
SMRi Q9CXY9. Positions 43-308.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9CXY9. 1 interaction.
STRINGi 10090.ENSMUSP00000045351.

Protein family/group databases

MEROPSi C13.005.

PTM databases

PhosphoSitei Q9CXY9.

Proteomic databases

MaxQBi Q9CXY9.
PaxDbi Q9CXY9.
PRIDEi Q9CXY9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000159899 ; ENSMUSP00000123772 ; ENSMUSG00000039047 .
GeneIDi 329777.
KEGGi mmu:329777.
UCSCi uc012czo.1. mouse.

Organism-specific databases

CTDi 10026.
MGIi MGI:1913863. Pigk.

Phylogenomic databases

eggNOGi COG5206.
GeneTreei ENSGT00530000063391.
HOGENOMi HOG000204398.
HOVERGENi HBG027488.
KOi K05290.
OrthoDBi EOG70W3DZ.

Enzyme and pathway databases

UniPathwayi UPA00196 .

Miscellaneous databases

NextBioi 398962.
PROi Q9CXY9.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9CXY9.
Bgeei Q9CXY9.
Genevestigatori Q9CXY9.

Family and domain databases

InterProi IPR028361. GPI_transamidase.
IPR001096. Peptidase_C13.
[Graphical view ]
PANTHERi PTHR12000. PTHR12000. 1 hit.
Pfami PF01650. Peptidase_C13. 1 hit.
[Graphical view ]
PIRSFi PIRSF500138. GPI8. 1 hit.
PIRSF019663. Legumain. 1 hit.
PRINTSi PR00776. HEMOGLOBNASE.
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic head and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiGPI8_MOUSE
AccessioniPrimary (citable) accession number: Q9CXY9
Secondary accession number(s): Q8BH63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: August 16, 2004
Last modified: July 9, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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