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Q9CXY6 (ILF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interleukin enhancer-binding factor 2
Alternative name(s):
Nuclear factor of activated T-cells 45 kDa
Gene names
Name:Ilf2
Synonyms:Nf45
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length390 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA By similarity. Essential for the efficient reshuttling of ILF3 into the nucleus By similarity. Ref.4

Subunit structure

Forms heterodimers with ILF3. ILF2-ILF3 heterodimers may also bind to PRKDC/XRCC7: this may stabilize the interaction of PRKDC/XRCC7 and the heterodimeric complex of G22P1/KU70 and XRCC5/KU80. Forms a complex with ILF3, YLPM1, KHDRBS1, RBMX, NCOA5 and PPP1CA. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with IGF2BP1 By similarity. Ref.4

Subcellular location

Nucleusnucleolus. Cytoplasm By similarity. Nucleus By similarity. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs By similarity. Ref.5

Tissue specificity

Expressed in brain, kidney and ovary; highly expressed in testis, particularly within pachytene cells. Ref.5

Developmental stage

Expression in testis begins with developmental differentiation of pachytene spermatocytes. Ref.5

Sequence similarities

Contains 1 DZF domain.

Sequence caution

The sequence BAC27594.1 differs from that shown. Reason: Intron retention.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 390390Interleukin enhancer-binding factor 2
PRO_0000126064

Regions

Domain24 – 371348DZF
Compositional bias366 – 3727Poly-Glu
Compositional bias381 – 3844Poly-Glu

Amino acid modifications

Modified residue3881Phosphothreonine By similarity

Secondary structure

.......................................................... 390
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9CXY6 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 75BAD022DCD4EE01

FASTA39043,062
        10         20         30         40         50         60 
MRGDRGRGRG GRFGSRGGPG GGFRPFVPHI PFDFYLCEMA FPRVKPAPDE TSFSEALLKR 

        70         80         90        100        110        120 
NQDLAPNSAE QASILSLVTK INNVIDNLIV APGTFEVQIE EVRQVGSYKK GTMTTGHNVA 

       130        140        150        160        170        180 
DLVVILKILP TLEAVAALGN KVVESLRAQD PSEVLTMLTN ETGFEISSSD ATVKILITTV 

       190        200        210        220        230        240 
PPNLRKLDPE LHLDIKVLQS ALAAIRHARW FEENASQSTV KVLIRLLKDL RIRFPGFEPL 

       250        260        270        280        290        300 
TPWILDLLGH YAVMNNPTRQ PLALNVAYRR CLQILAAGLF LPGSVGITDP CESGNFRVHT 

       310        320        330        340        350        360 
VMTLEQQDMV CYTAQTLVRI LSHGGFRKIL GQEGDASYLA SEISTWDGVI VTPSEKAYEK 

       370        380        390 
PPEKKEGEEE EENTEEPPQG EEEESMETQE 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of cyclosporin A- and FK506-sensitive nuclear factor of activated T-cells: NF45 and NF90."
Kao P.N., Chen L., Brock G., Ng J., Kenny J., Smith A.J., Corthesy B.
J. Biol. Chem. 269:20691-20699(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J and NOD.
Tissue: Head, Medulla oblongata and Spleen.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary tumor.
[4]"Autoantibodies define a family of proteins with conserved double-stranded RNA-binding domains as well as DNA binding activity."
Satoh M., Shaheen V.M., Kao P.N., Okano T., Shaw M., Yoshida H., Richards H.B., Reeves W.H.
J. Biol. Chem. 274:34598-34604(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ILF3.
[5]"Ilf2 is regulated during meiosis and associated to transcriptionally active chromatin."
Lopez-Fernandez L.A., Parraga M., del Mazo J.
Mech. Dev. 111:153-157(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF458249 Genomic DNA. Translation: AAL59388.1.
AK013858 mRNA. Translation: BAB29021.1.
AK031892 mRNA. Translation: BAC27594.1. Sequence problems.
AK078003 mRNA. Translation: BAC37097.1.
AK157129 mRNA. Translation: BAE33972.1.
BC004033 mRNA. Translation: AAH04033.1.
BC024718 mRNA. Translation: AAH24718.1.
CCDSCCDS17530.1.
RefSeqNP_080650.1. NM_026374.3.
UniGeneMm.227258.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4AT7X-ray1.90A29-390[»]
4AT8X-ray2.69A/C29-390[»]
4AT9X-ray2.80A29-390[»]
4ATBX-ray3.10A/C29-390[»]
ProteinModelPortalQ9CXY6.
SMRQ9CXY6. Positions 29-361.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid212438. 4 interactions.
IntActQ9CXY6. 4 interactions.
MINTMINT-4125797.

PTM databases

PhosphoSiteQ9CXY6.

Proteomic databases

MaxQBQ9CXY6.
PaxDbQ9CXY6.
PRIDEQ9CXY6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000001042; ENSMUSP00000001042; ENSMUSG00000001016.
GeneID67781.
KEGGmmu:67781.
UCSCuc008qcj.1. mouse.

Organism-specific databases

CTD3608.
MGIMGI:1915031. Ilf2.

Phylogenomic databases

eggNOGNOG247512.
GeneTreeENSGT00550000074528.
HOGENOMHOG000067801.
HOVERGENHBG052120.
InParanoidQ9CXY6.
KOK13089.
OMAFEENAHH.
OrthoDBEOG75XGM4.
PhylomeDBQ9CXY6.
TreeFamTF320194.

Gene expression databases

BgeeQ9CXY6.
CleanExMM_ILF2.
GenevestigatorQ9CXY6.

Family and domain databases

InterProIPR006116. 2-5-oligoadenylate_synth_N.
IPR006561. DZF.
[Graphical view]
PfamPF07528. DZF. 1 hit.
[Graphical view]
SMARTSM00572. DZF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSILF2. mouse.
NextBio325549.
PROQ9CXY6.
SOURCESearch...

Entry information

Entry nameILF2_MOUSE
AccessionPrimary (citable) accession number: Q9CXY6
Secondary accession number(s): Q3U083 expand/collapse secondary AC list , Q5RKG0, Q8CCY9, Q99KS3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot