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Protein

Interleukin enhancer-binding factor 2

Gene

Ilf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Appears to function predominantly as a heterodimeric complex with ILF3. This complex may regulate transcription of the IL2 gene during T-cell activation. It can also promote the formation of stable DNA-dependent protein kinase holoenzyme complexes on DNA (By similarity). Essential for the efficient reshuttling of ILF3 into the nucleus (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin enhancer-binding factor 2
Alternative name(s):
Nuclear factor of activated T-cells 45 kDa
Gene namesi
Name:Ilf2
Synonyms:Nf45
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1915031. Ilf2.

Subcellular locationi

  • Nucleusnucleolus 1 Publication
  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001260641 – 390Interleukin enhancer-binding factor 2Add BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei16Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei16Omega-N-methylarginine; alternateCombined sources1
Modified residuei24Omega-N-methylarginineCombined sources1
Modified residuei52PhosphoserineBy similarity1
Modified residuei68PhosphoserineBy similarity1
Modified residuei388PhosphothreonineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ9CXY6.
MaxQBiQ9CXY6.
PaxDbiQ9CXY6.
PRIDEiQ9CXY6.

PTM databases

iPTMnetiQ9CXY6.
PhosphoSitePlusiQ9CXY6.

Expressioni

Tissue specificityi

Expressed in brain, kidney and ovary; highly expressed in testis, particularly within pachytene cells.1 Publication

Developmental stagei

Expression in testis begins with developmental differentiation of pachytene spermatocytes.1 Publication

Gene expression databases

BgeeiENSMUSG00000001016.
CleanExiMM_ILF2.
GenevisibleiQ9CXY6. MM.

Interactioni

Subunit structurei

Forms heterodimers with ILF3. ILF2-ILF3 heterodimers may also bind to PRKDC/XRCC7: this may stabilize the interaction of PRKDC/XRCC7 and the heterodimeric complex of G22P1/KU70 and XRCC5/KU80. Forms a complex with ILF3, YLPM1, KHDRBS1, RBMX, NCOA5 and PPP1CA. Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with IGF2BP1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi212438. 5 interactors.
IntActiQ9CXY6. 4 interactors.
MINTiMINT-4125797.
STRINGi10090.ENSMUSP00000001042.

Structurei

Secondary structure

1390
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi34 – 36Combined sources3
Helixi38 – 40Combined sources3
Helixi51 – 64Combined sources14
Helixi68 – 90Combined sources23
Turni94 – 98Combined sources5
Beta strandi99 – 105Combined sources7
Helixi106 – 110Combined sources5
Beta strandi119 – 128Combined sources10
Helixi132 – 149Combined sources18
Beta strandi151 – 153Combined sources3
Beta strandi156 – 160Combined sources5
Beta strandi163 – 167Combined sources5
Beta strandi172 – 179Combined sources8
Helixi181 – 185Combined sources5
Turni189 – 191Combined sources3
Helixi195 – 214Combined sources20
Helixi218 – 233Combined sources16
Helixi235 – 237Combined sources3
Helixi242 – 254Combined sources13
Beta strandi256 – 260Combined sources5
Helixi264 – 276Combined sources13
Turni277 – 280Combined sources4
Beta strandi292 – 294Combined sources3
Helixi298 – 301Combined sources4
Helixi304 – 322Combined sources19
Helixi326 – 329Combined sources4
Beta strandi332 – 334Combined sources3
Helixi337 – 340Combined sources4
Beta strandi344 – 346Combined sources3
Beta strandi349 – 351Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AT7X-ray1.90A29-390[»]
4AT8X-ray2.69A/C29-390[»]
4AT9X-ray2.80A29-390[»]
4ATBX-ray3.10A/C29-390[»]
ProteinModelPortaliQ9CXY6.
SMRiQ9CXY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 371DZFPROSITE-ProRule annotationAdd BLAST348

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi366 – 372Poly-Glu7
Compositional biasi381 – 384Poly-Glu4

Sequence similaritiesi

Contains 1 DZF domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3793. Eukaryota.
ENOG410XQCJ. LUCA.
GeneTreeiENSGT00550000074528.
HOGENOMiHOG000067801.
HOVERGENiHBG052120.
InParanoidiQ9CXY6.
KOiK13089.
OMAiYEKPPER.
OrthoDBiEOG091G0HP1.
PhylomeDBiQ9CXY6.
TreeFamiTF320194.

Family and domain databases

InterProiIPR006116. 2-5-oligoadenylate_synth_N.
IPR006561. DZF_dom.
[Graphical view]
PfamiPF07528. DZF. 1 hit.
[Graphical view]
SMARTiSM00572. DZF. 1 hit.
[Graphical view]
PROSITEiPS51703. DZF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CXY6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGDRGRGRG GRFGSRGGPG GGFRPFVPHI PFDFYLCEMA FPRVKPAPDE
60 70 80 90 100
TSFSEALLKR NQDLAPNSAE QASILSLVTK INNVIDNLIV APGTFEVQIE
110 120 130 140 150
EVRQVGSYKK GTMTTGHNVA DLVVILKILP TLEAVAALGN KVVESLRAQD
160 170 180 190 200
PSEVLTMLTN ETGFEISSSD ATVKILITTV PPNLRKLDPE LHLDIKVLQS
210 220 230 240 250
ALAAIRHARW FEENASQSTV KVLIRLLKDL RIRFPGFEPL TPWILDLLGH
260 270 280 290 300
YAVMNNPTRQ PLALNVAYRR CLQILAAGLF LPGSVGITDP CESGNFRVHT
310 320 330 340 350
VMTLEQQDMV CYTAQTLVRI LSHGGFRKIL GQEGDASYLA SEISTWDGVI
360 370 380 390
VTPSEKAYEK PPEKKEGEEE EENTEEPPQG EEEESMETQE
Length:390
Mass (Da):43,062
Last modified:June 1, 2001 - v1
Checksum:i75BAD022DCD4EE01
GO

Sequence cautioni

The sequence BAC27594 differs from that shown. Intron retention.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF458249 Genomic DNA. Translation: AAL59388.1.
AK013858 mRNA. Translation: BAB29021.1.
AK031892 mRNA. Translation: BAC27594.1. Sequence problems.
AK078003 mRNA. Translation: BAC37097.1.
AK157129 mRNA. Translation: BAE33972.1.
BC004033 mRNA. Translation: AAH04033.1.
BC024718 mRNA. Translation: AAH24718.1.
CCDSiCCDS17530.1.
RefSeqiNP_080650.1. NM_026374.3.
UniGeneiMm.227258.

Genome annotation databases

EnsembliENSMUST00000001042; ENSMUSP00000001042; ENSMUSG00000001016.
GeneIDi67781.
KEGGimmu:67781.
UCSCiuc008qcj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF458249 Genomic DNA. Translation: AAL59388.1.
AK013858 mRNA. Translation: BAB29021.1.
AK031892 mRNA. Translation: BAC27594.1. Sequence problems.
AK078003 mRNA. Translation: BAC37097.1.
AK157129 mRNA. Translation: BAE33972.1.
BC004033 mRNA. Translation: AAH04033.1.
BC024718 mRNA. Translation: AAH24718.1.
CCDSiCCDS17530.1.
RefSeqiNP_080650.1. NM_026374.3.
UniGeneiMm.227258.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4AT7X-ray1.90A29-390[»]
4AT8X-ray2.69A/C29-390[»]
4AT9X-ray2.80A29-390[»]
4ATBX-ray3.10A/C29-390[»]
ProteinModelPortaliQ9CXY6.
SMRiQ9CXY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212438. 5 interactors.
IntActiQ9CXY6. 4 interactors.
MINTiMINT-4125797.
STRINGi10090.ENSMUSP00000001042.

PTM databases

iPTMnetiQ9CXY6.
PhosphoSitePlusiQ9CXY6.

Proteomic databases

EPDiQ9CXY6.
MaxQBiQ9CXY6.
PaxDbiQ9CXY6.
PRIDEiQ9CXY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001042; ENSMUSP00000001042; ENSMUSG00000001016.
GeneIDi67781.
KEGGimmu:67781.
UCSCiuc008qcj.1. mouse.

Organism-specific databases

CTDi3608.
MGIiMGI:1915031. Ilf2.

Phylogenomic databases

eggNOGiKOG3793. Eukaryota.
ENOG410XQCJ. LUCA.
GeneTreeiENSGT00550000074528.
HOGENOMiHOG000067801.
HOVERGENiHBG052120.
InParanoidiQ9CXY6.
KOiK13089.
OMAiYEKPPER.
OrthoDBiEOG091G0HP1.
PhylomeDBiQ9CXY6.
TreeFamiTF320194.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiIlf2. mouse.
PROiQ9CXY6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000001016.
CleanExiMM_ILF2.
GenevisibleiQ9CXY6. MM.

Family and domain databases

InterProiIPR006116. 2-5-oligoadenylate_synth_N.
IPR006561. DZF_dom.
[Graphical view]
PfamiPF07528. DZF. 1 hit.
[Graphical view]
SMARTiSM00572. DZF. 1 hit.
[Graphical view]
PROSITEiPS51703. DZF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiILF2_MOUSE
AccessioniPrimary (citable) accession number: Q9CXY6
Secondary accession number(s): Q3U083
, Q5RKG0, Q8CCY9, Q99KS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.