Q9CXW3 (CYBP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcyclin-binding protein
Short name=CacyBP

Alternative name(s):
Siah-interacting protein

Gene names

Name:	Cacybp
Synonyms:	Sip

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1) By similarity.
Subunit structure	Monomer or homodimer. Component of some large E3 complex at least composed of UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts directly with SIAH1, SIAH2 and SKP1 By similarity. Interacts with proteins of the S100 family S100A1, S100A6, S100B, S100P and S100A12 at physiological calcium concentrations. Ref.1 Ref.4 Ref.6 Ref.7 Ref.8
Subcellular location	Nucleus. Cytoplasm. Note: Cytoplasmic in unstimulated cultured neurons. Upon increase of calcium, it localizes to a ring around the nucleus. In neuroblastoma cells, after a Retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear and perinuclear fractions may be phosphorylated. Ref.5
Tissue specificity	Highly expressed in brain and EAT cells. Expressed at low level in heart, muscle, and at very low level in the liver, spleen, lung, kidney and stomach. Ref.1
Post-translational modification	Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations. Ref.5
Sequence similarities	Contains 1 CS domain. Contains 1 SGS domain.
Sequence caution	The sequence AAC16757.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Cytoplasm Nucleus
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	beta-catenin destruction complex Inferred from sequence or structural similarity. Source: UniProtKB nuclear envelope lumen Inferred from direct assay Ref.5. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 229

228

Calcyclin-binding protein

PRO_0000185390

Regions

Domain

74 – 168

95

CS

Domain

169 – 229

61

SGS

Region

2 – 81

80

Interaction with SIAH1 By similarity

Region

74 – 229

156

Interaction with SKP1 By similarity

Region

155 – 229

75

Interaction with S100A6

Amino acid modifications

Modified residue

2

1

N-acetylalanine By similarity

Modified residue

10

1

N6-acetyllysine By similarity

Modified residue

21

1

N6-acetyllysine By similarity

Modified residue

86

1

N6-acetyllysine By similarity

Modified residue

119

1

N6-acetyllysine By similarity

Modified residue

135

1

N6-acetyllysine By similarity

Modified residue

210

1

Phosphothreonine By similarity

Secondary structure

1

.

.

.

.

.

229

Helix Strand Turn

Details...

Helix

2 – 21

20

Turn

22 – 26

5

Helix

27 – 47

21

Sequences

	Sequence		Length	Mass (Da)
	Q9CXW3 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 2B04B54D0054ACE0	FASTA	229	26,510
	10 20 30 40 50 60 MASVLEELQK DLEEVKVLLE KSTRKRLRDT LTSEKSKIET ELKNKMQQKS QKKPELDNEK 70 80 90 100 110 120 PAAVVAPLTT GYTVKISNYG WDQSDKFVKI YITLTGVHQV PTENVQVHFT ERSFDLLVKN 130 140 150 160 170 180 LNGKNYSMIV NNLLKPISVE SSSKKVKTDT VIILCRKKAE NTRWDYLTQV EKECKEKEKP 190 200 210 220 SYDTEADPSE GLMNVLKKIY EDGDDDMKRT INKAWVESRE KQAREDTEF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of a mouse brain cDNA encoding a novel protein target of calcyclin." Filipek A., Kuznicki J. J. Neurochem. 70:1793-1798(1998) [PubMed: 9572262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-22; 73-85 AND 222-229, TISSUE SPECIFICITY, INTERACTION WITH S100A6. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Characterization of the interaction of calcyclin (S100A6) and calcyclin-binding protein." Nowotny M., Bhattacharya S., Filipek A., Krezel A.M., Chazin W., Kuznicki J. J. Biol. Chem. 275:31178-31182(2000) [PubMed: 10884380] [Abstract] Cited for: INTERACTION WITH S100A6.
[5]	"Ca2+-dependent translocation of the calcyclin-binding protein in neurons and neuroblastoma NB-2a cells." Filipek A., Jastrzebska B., Nowotny M., Kwiatkowska K., Hetman M., Surmacz L., Wyroba E., Kuznicki J. J. Biol. Chem. 277:21103-21109(2002) [PubMed: 11927578] [Abstract] Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.
[6]	"CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family." Filipek A., Jastrzebska B., Nowotny M., Kuznicki J. J. Biol. Chem. 277:28848-28852(2002) [PubMed: 12042313] [Abstract] Cited for: INTERACTION WITH S100A1; S100A6; S100B; S100P AND S100A12.
[7]	"The modular structure of SIP facilitates its role in stabilizing multiprotein assemblies." Bhattacharya S., Lee Y.-T., Michowski W., Jastrzebska B., Filipek A., Kuznicki J., Chazin W.J. Biochemistry 44:9462-9471(2005) [PubMed: 15996101] [Abstract] Cited for: STRUCTURE BY NMR OF 1-77, INTERACTION WITH SKP1 AND S100A6, SUBUNIT.
[8]	"Structure of the S100A6 complex with a fragment from the C-terminal domain of Siah-1 interacting protein: a novel mode for S100 protein target recognition." Lee Y.-T., Dimitrova Y.N., Schneider G., Ridenour W.B., Bhattacharya S., Soss S.E., Caprioli R.M., Filipek A., Chazin W.J. Biochemistry 47:10921-10932(2008) [PubMed: 18803400] [Abstract] Cited for: STRUCTURE BY NMR OF 189-219 IN COMPLEX WITH S100A6, INTERACTION WITH S100A6.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U97327 mRNA. Translation: AAC16757.1. Different initiation.
AK013924 mRNA. No translation available.
BC025948 mRNA. Translation: AAH25948.1.

IPI

IPI00115650.

RefSeq

NP_033916.1. NM_009786.2.

UniGene

Mm.10702.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YSM	NMR	-	A	1-77	[»]
2JTT	NMR	-	C/D	189-219	[»]

ProteinModelPortal

Q9CXW3.

SMR

Q9CXW3. Positions 1-55, 62-181, 189-219.

DisProt

DP00226.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9CXW3. 1 interaction.

STRING

Q9CXW3.

PTM databases

PhosphoSite

Q9CXW3.

2D gel databases

REPRODUCTION-2DPAGE

Q9CXW3.

Proteomic databases

PRIDE

Q9CXW3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000014370; ENSMUSP00000014370; ENSMUSG00000014226.

GeneID

12301.

KEGG

mmu:12301.

NMPDR

fig|10090.3.peg.1314.

UCSC

uc007deg.1. mouse.

Organism-specific databases

CTD

27101.

MGI

MGI:1270839. Cacybp.

Phylogenomic databases

eggNOG

roNOG05191.

GeneTree

ENSGT00390000016470.

HOGENOM

HBG627208.

HOVERGEN

HBG003242.

InParanoid

Q9CXW3.

OMA

YAWDQSD.

OrthoDB

EOG4Q2DGC.

PhylomeDB

Q9CXW3.

Gene expression databases

ArrayExpress

Q9CXW3.

Bgee

Q9CXW3.

CleanEx

MM_CACYBP.

Genevestigator

Q9CXW3.

GermOnline

ENSMUSG00000014226. Mus musculus.

Family and domain databases

InterPro

IPR007052. CS-like_domain.
IPR017447. CS_domain.
IPR008978. HSP20-like_chaperone.
IPR007699. SGS.
IPR015120. Siah-Interact_N.
[Graphical view]

KO

K04507.

Pfam

PF04969. CS. 1 hit.
PF05002. SGS. 1 hit.
PF09032. Siah-Interact_N. 1 hit.
[Graphical view]

SUPFAM

SSF49764. HSP20_chap. 1 hit.

PROSITE

PS51203. CS. 1 hit.
PS51048. SGS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

280826.

SOURCE

Search...

Entry information

Entry name

CYBP_MOUSE

Accession

Primary (citable) accession number: Q9CXW3
Secondary accession number(s): O70140

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2001
Last modified:	November 16, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program