Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcyclin-binding protein

Gene

Cacybp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1) (By similarity).By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Calcyclin-binding protein
Short name:
CacyBP
Alternative name(s):
Siah-interacting protein
Gene namesi
Name:Cacybp
Synonyms:Sip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1270839. Cacybp.

Subcellular locationi

GO - Cellular componenti

  • beta-catenin destruction complex Source: UniProtKB
  • cytoplasm Source: MGI
  • extracellular exosome Source: MGI
  • nuclear envelope lumen Source: MGI
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 229228Calcyclin-binding proteinPRO_0000185390Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei3 – 31PhosphoserineBy similarity
Modified residuei10 – 101N6-acetyllysineBy similarity
Modified residuei21 – 211N6-acetyllysineBy similarity
Modified residuei36 – 361PhosphoserineBy similarity
Modified residuei86 – 861N6-acetyllysineBy similarity
Modified residuei119 – 1191N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9CXW3.
MaxQBiQ9CXW3.
PaxDbiQ9CXW3.
PeptideAtlasiQ9CXW3.
PRIDEiQ9CXW3.
TopDownProteomicsiQ9CXW3.

2D gel databases

REPRODUCTION-2DPAGEQ9CXW3.

PTM databases

iPTMnetiQ9CXW3.
PhosphoSiteiQ9CXW3.
SwissPalmiQ9CXW3.

Expressioni

Tissue specificityi

Highly expressed in brain and EAT cells. Expressed at low level in heart, muscle, and at very low level in the liver, spleen, lung, kidney and stomach.1 Publication

Gene expression databases

BgeeiENSMUSG00000014226.
CleanExiMM_CACYBP.
ExpressionAtlasiQ9CXW3. baseline and differential.
GenevisibleiQ9CXW3. MM.

Interactioni

Subunit structurei

Monomer or homodimer. Component of some large E3 complex at least composed of UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X. Interacts directly with SIAH1, SIAH2 and SKP1 (By similarity). Interacts with proteins of the S100 family S100A1, S100A6, S100B, S100P and S100A12 in a calcium-dependent manner (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
S100A1P026394EBI-767146,EBI-6477285From a different organism.
S100a6P140694EBI-767146,EBI-6478740
S100BP026384EBI-767146,EBI-458452From a different organism.
S100PP258152EBI-767146,EBI-743700From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198445. 11 interactions.
IntActiQ9CXW3. 11 interactions.
MINTiMINT-238999.
STRINGi10090.ENSMUSP00000014370.

Structurei

Secondary structure

1
229
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 2120Combined sources
Turni22 – 265Combined sources
Helixi27 – 4721Combined sources
Helixi192 – 2009Combined sources
Helixi207 – 21610Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YSMNMR-A1-77[»]
2JTTNMR-C/D189-219[»]
DisProtiDP00226.
ProteinModelPortaliQ9CXW3.
SMRiQ9CXW3. Positions 1-55, 62-181, 189-219.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9CXW3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini74 – 16895CSPROSITE-ProRule annotationAdd
BLAST
Domaini169 – 22961SGSPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 8180Interaction with SIAH1By similarityAdd
BLAST
Regioni74 – 229156Interaction with SKP1By similarityAdd
BLAST
Regioni155 – 22975Interaction with S100A6Add
BLAST

Sequence similaritiesi

Contains 1 CS domain.PROSITE-ProRule annotation
Contains 1 SGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3260. Eukaryota.
ENOG410XSB6. LUCA.
GeneTreeiENSGT00390000016470.
HOGENOMiHOG000238284.
HOVERGENiHBG003242.
InParanoidiQ9CXW3.
KOiK04507.
OMAiMKKMYND.
OrthoDBiEOG091G0LQC.
PhylomeDBiQ9CXW3.
TreeFamiTF323891.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR007699. SGS_dom.
IPR015120. Siah-Interact_N.
[Graphical view]
PfamiPF04969. CS. 1 hit.
PF05002. SGS. 1 hit.
PF09032. Siah-Interact_N. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51203. CS. 1 hit.
PS51048. SGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CXW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVLEELQK DLEEVKVLLE KSTRKRLRDT LTSEKSKIET ELKNKMQQKS
60 70 80 90 100
QKKPELDNEK PAAVVAPLTT GYTVKISNYG WDQSDKFVKI YITLTGVHQV
110 120 130 140 150
PTENVQVHFT ERSFDLLVKN LNGKNYSMIV NNLLKPISVE SSSKKVKTDT
160 170 180 190 200
VIILCRKKAE NTRWDYLTQV EKECKEKEKP SYDTEADPSE GLMNVLKKIY
210 220
EDGDDDMKRT INKAWVESRE KQAREDTEF
Length:229
Mass (Da):26,510
Last modified:June 1, 2001 - v1
Checksum:i2B04B54D0054ACE0
GO

Sequence cautioni

The sequence AAC16757 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97327 mRNA. Translation: AAC16757.1. Different initiation.
AK013924 mRNA. No translation available.
BC025948 mRNA. Translation: AAH25948.1.
CCDSiCCDS15406.1.
RefSeqiNP_033916.1. NM_009786.2.
UniGeneiMm.10702.

Genome annotation databases

EnsembliENSMUST00000014370; ENSMUSP00000014370; ENSMUSG00000014226.
GeneIDi12301.
KEGGimmu:12301.
UCSCiuc007deg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97327 mRNA. Translation: AAC16757.1. Different initiation.
AK013924 mRNA. No translation available.
BC025948 mRNA. Translation: AAH25948.1.
CCDSiCCDS15406.1.
RefSeqiNP_033916.1. NM_009786.2.
UniGeneiMm.10702.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1YSMNMR-A1-77[»]
2JTTNMR-C/D189-219[»]
DisProtiDP00226.
ProteinModelPortaliQ9CXW3.
SMRiQ9CXW3. Positions 1-55, 62-181, 189-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198445. 11 interactions.
IntActiQ9CXW3. 11 interactions.
MINTiMINT-238999.
STRINGi10090.ENSMUSP00000014370.

PTM databases

iPTMnetiQ9CXW3.
PhosphoSiteiQ9CXW3.
SwissPalmiQ9CXW3.

2D gel databases

REPRODUCTION-2DPAGEQ9CXW3.

Proteomic databases

EPDiQ9CXW3.
MaxQBiQ9CXW3.
PaxDbiQ9CXW3.
PeptideAtlasiQ9CXW3.
PRIDEiQ9CXW3.
TopDownProteomicsiQ9CXW3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014370; ENSMUSP00000014370; ENSMUSG00000014226.
GeneIDi12301.
KEGGimmu:12301.
UCSCiuc007deg.2. mouse.

Organism-specific databases

CTDi27101.
MGIiMGI:1270839. Cacybp.

Phylogenomic databases

eggNOGiKOG3260. Eukaryota.
ENOG410XSB6. LUCA.
GeneTreeiENSGT00390000016470.
HOGENOMiHOG000238284.
HOVERGENiHBG003242.
InParanoidiQ9CXW3.
KOiK04507.
OMAiMKKMYND.
OrthoDBiEOG091G0LQC.
PhylomeDBiQ9CXW3.
TreeFamiTF323891.

Miscellaneous databases

EvolutionaryTraceiQ9CXW3.
PROiQ9CXW3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000014226.
CleanExiMM_CACYBP.
ExpressionAtlasiQ9CXW3. baseline and differential.
GenevisibleiQ9CXW3. MM.

Family and domain databases

Gene3Di2.60.40.790. 1 hit.
InterProiIPR007052. CS_dom.
IPR008978. HSP20-like_chaperone.
IPR007699. SGS_dom.
IPR015120. Siah-Interact_N.
[Graphical view]
PfamiPF04969. CS. 1 hit.
PF05002. SGS. 1 hit.
PF09032. Siah-Interact_N. 1 hit.
[Graphical view]
SUPFAMiSSF49764. SSF49764. 1 hit.
PROSITEiPS51203. CS. 1 hit.
PS51048. SGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYBP_MOUSE
AccessioniPrimary (citable) accession number: Q9CXW3
Secondary accession number(s): O70140
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.