Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Insulin gene enhancer protein ISL-2

Gene

Isl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi191 – 25060HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • negative regulation of neuron differentiation Source: MGI
  • neuron fate commitment Source: MGI
  • regulation of transcription, DNA-templated Source: InterPro
  • retinal ganglion cell axon guidance Source: MGI
  • spinal cord motor neuron cell fate specification Source: MGI
  • visceral motor neuron differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin gene enhancer protein ISL-2
Short name:
Islet-2
Gene namesi
Name:Isl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:109156. Isl2.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Insulin gene enhancer protein ISL-2PRO_0000075753Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei154 – 1541PhosphoserineBy similarity
Modified residuei157 – 1571PhosphoserineBy similarity
Modified residuei279 – 2791PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9CXV0.
PRIDEiQ9CXV0.

PTM databases

iPTMnetiQ9CXV0.
PhosphoSiteiQ9CXV0.

Expressioni

Gene expression databases

CleanExiMM_ISL2.
ExpressionAtlasiQ9CXV0. baseline and differential.
GenevisibleiQ9CXV0. MM.

Interactioni

Subunit structurei

Interacts with LHX4.1 Publication

Protein-protein interaction databases

BioGridi222577. 8 interactions.
STRINGi10090.ENSMUSP00000034869.

Structurei

Secondary structure

1
359
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi272 – 2765Combined sources
Helixi283 – 2864Combined sources
Beta strandi290 – 2967Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MMKX-ray2.16A/B272-301[»]
ProteinModelPortaliQ9CXV0.
SMRiQ9CXV0. Positions 25-143, 190-254, 272-301.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 8662LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini87 – 14963LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni272 – 30130LIM-binding domain (LID)Add
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 2 LIM zinc-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox, LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118921.
HOGENOMiHOG000236304.
HOVERGENiHBG004671.
InParanoidiQ9CXV0.
KOiK18492.
OMAiRHDSAVQ.
TreeFamiTF315442.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CXV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDIIFHYPF LGAMGDHSKK KPGTAMCVGC GSQIHDQFIL RVSPDLEWHA
60 70 80 90 100
ACLKCAECSQ YLDETCTCFV RDGKTYCKRD YVRLFGIKCA QCQVGFSSSD
110 120 130 140 150
LVMRARDSVY HIECFRCSVC SRQLLPGDEF SLREHELLCR ADHGLLLERA
160 170 180 190 200
AAGSPRSPGP LPGARGLHLP DAGSGRQPSL RTHVHKQAEK TTRVRTVLNE
210 220 230 240 250
KQLHTLRTCY AANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKKS
260 270 280 290 300
ILMKQLQQQQ HSDKASLQGL TGTPLVAGSP IRHENAVQGS AVEVQTYQPP
310 320 330 340 350
WKALSEFALQ SDLDQPAFQQ LVSFSESGSL GNSSGSDVTS LSSQLPDTPN

SMVPSPVET
Length:359
Mass (Da):39,728
Last modified:October 3, 2012 - v2
Checksum:iA3745127E6C5FD61
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti157 – 1571S → T in BAB29088 (PubMed:16141072).Curated
Sequence conflicti185 – 1851H → D in BAB29088 (PubMed:16141072).Curated
Sequence conflicti267 – 2671L → F in BAB29088 (PubMed:16141072).Curated
Sequence conflicti282 – 2821R → G in BAB29088 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013964 mRNA. Translation: BAB29088.1.
AC116699 Genomic DNA. No translation available.
CH466522 Genomic DNA. Translation: EDL25854.1.
CCDSiCCDS23205.1.
RefSeqiNP_081673.2. NM_027397.3.
UniGeneiMm.273996.

Genome annotation databases

EnsembliENSMUST00000034869; ENSMUSP00000034869; ENSMUSG00000032318.
GeneIDi104360.
KEGGimmu:104360.
UCSCiuc009psm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013964 mRNA. Translation: BAB29088.1.
AC116699 Genomic DNA. No translation available.
CH466522 Genomic DNA. Translation: EDL25854.1.
CCDSiCCDS23205.1.
RefSeqiNP_081673.2. NM_027397.3.
UniGeneiMm.273996.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MMKX-ray2.16A/B272-301[»]
ProteinModelPortaliQ9CXV0.
SMRiQ9CXV0. Positions 25-143, 190-254, 272-301.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi222577. 8 interactions.
STRINGi10090.ENSMUSP00000034869.

PTM databases

iPTMnetiQ9CXV0.
PhosphoSiteiQ9CXV0.

Proteomic databases

PaxDbiQ9CXV0.
PRIDEiQ9CXV0.

Protocols and materials databases

DNASUi104360.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034869; ENSMUSP00000034869; ENSMUSG00000032318.
GeneIDi104360.
KEGGimmu:104360.
UCSCiuc009psm.1. mouse.

Organism-specific databases

CTDi64843.
MGIiMGI:109156. Isl2.

Phylogenomic databases

eggNOGiKOG0490. Eukaryota.
ENOG410YIJ3. LUCA.
GeneTreeiENSGT00760000118921.
HOGENOMiHOG000236304.
HOVERGENiHBG004671.
InParanoidiQ9CXV0.
KOiK18492.
OMAiRHDSAVQ.
TreeFamiTF315442.

Miscellaneous databases

PROiQ9CXV0.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ISL2.
ExpressionAtlasiQ9CXV0. baseline and differential.
GenevisibleiQ9CXV0. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
2.10.110.10. 2 hits.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00412. LIM. 2 hits.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
SM00132. LIM. 2 hits.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic head.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Structural basis for partial redundancy in a class of transcription factors, the LIM homeodomain proteins, in neural cell type specification."
    Gadd M.S., Bhati M., Jeffries C.M., Langley D.B., Trewhella J., Guss J.M., Matthews J.M.
    J. Biol. Chem. 286:42971-42980(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.16 ANGSTROMS) OF 272-301 IN COMPLEX WITH LHX4.

Entry informationi

Entry nameiISL2_MOUSE
AccessioniPrimary (citable) accession number: Q9CXV0
Secondary accession number(s): G5E888
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: October 3, 2012
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.