ID   MED31_MOUSE             Reviewed;         131 AA.
AC   Q9CXU1; Q9DAP5;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2003, sequence version 2.
DT   27-MAR-2024, entry version 153.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 31;
DE   AltName: Full=Mediator complex subunit 31;
DE   AltName: Full=Mediator complex subunit SOH1;
GN   Name=Med31; Synonyms=Soh1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Embryo, and Testis;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Brain, and Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Liver, Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC       the regulated transcription of nearly all RNA polymerase II-dependent
CC       genes. Mediator functions as a bridge to convey information from gene-
CC       specific regulatory proteins to the basal RNA polymerase II
CC       transcription machinery. Mediator is recruited to promoters by direct
CC       interactions with regulatory proteins and serves as a scaffold for the
CC       assembly of a functional preinitiation complex with RNA polymerase II
CC       and the general transcription factors (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC       MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC       MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC       MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC       CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC       module termed the CDK8 module. Mediator containing the CDK8 module is
CC       less active than Mediator lacking this module in supporting
CC       transcriptional activation. Individual preparations of the Mediator
CC       complex lacking one or more distinct subunits have been variously
CC       termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).
CC       {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q9CXU1; Q8C1S0: Med19; NbExp=2; IntAct=EBI-309355, EBI-398761;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 31 family.
CC       {ECO:0000305}.
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DR   EMBL; AK005655; BAB24169.1; -; mRNA.
DR   EMBL; AK013990; BAB29102.1; -; mRNA.
DR   EMBL; BX119911; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC019685; AAH19685.1; -; mRNA.
DR   EMBL; BC051929; AAH51929.1; -; mRNA.
DR   CCDS; CCDS24981.1; -.
DR   RefSeq; NP_080344.2; NM_026068.2.
DR   PDB; 6W1S; EM; 4.02 A; Z=13-105.
DR   PDBsum; 6W1S; -.
DR   AlphaFoldDB; Q9CXU1; -.
DR   EMDB; EMD-21514; -.
DR   SMR; Q9CXU1; -.
DR   BioGRID; 212068; 2.
DR   ComplexPortal; CPX-3264; Core mediator complex.
DR   IntAct; Q9CXU1; 10.
DR   MINT; Q9CXU1; -.
DR   STRING; 10090.ENSMUSP00000021157; -.
DR   iPTMnet; Q9CXU1; -.
DR   PhosphoSitePlus; Q9CXU1; -.
DR   EPD; Q9CXU1; -.
DR   MaxQB; Q9CXU1; -.
DR   PaxDb; 10090-ENSMUSP00000021157; -.
DR   PeptideAtlas; Q9CXU1; -.
DR   ProteomicsDB; 293453; -.
DR   Pumba; Q9CXU1; -.
DR   Antibodypedia; 23820; 140 antibodies from 25 providers.
DR   DNASU; 67279; -.
DR   Ensembl; ENSMUST00000021157.9; ENSMUSP00000021157.9; ENSMUSG00000020801.9.
DR   GeneID; 67279; -.
DR   KEGG; mmu:67279; -.
DR   UCSC; uc007jyk.1; mouse.
DR   AGR; MGI:1914529; -.
DR   CTD; 51003; -.
DR   MGI; MGI:1914529; Med31.
DR   VEuPathDB; HostDB:ENSMUSG00000020801; -.
DR   eggNOG; KOG4086; Eukaryota.
DR   GeneTree; ENSGT00390000015531; -.
DR   HOGENOM; CLU_071681_5_1_1; -.
DR   InParanoid; Q9CXU1; -.
DR   OMA; VNSQCTK; -.
DR   OrthoDB; 306448at2759; -.
DR   PhylomeDB; Q9CXU1; -.
DR   TreeFam; TF105799; -.
DR   BioGRID-ORCS; 67279; 21 hits in 80 CRISPR screens.
DR   ChiTaRS; Med31; mouse.
DR   PRO; PR:Q9CXU1; -.
DR   Proteomes; UP000000589; Chromosome 11.
DR   RNAct; Q9CXU1; Protein.
DR   Bgee; ENSMUSG00000020801; Expressed in endocardial cushion and 248 other cell types or tissues.
DR   GO; GO:0070847; C:core mediator complex; ISO:MGI.
DR   GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR   GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR   GO; GO:0005634; C:nucleus; ISO:MGI.
DR   GO; GO:0000151; C:ubiquitin ligase complex; ISO:MGI.
DR   GO; GO:0003712; F:transcription coregulator activity; IEA:InterPro.
DR   GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:Ensembl.
DR   GO; GO:0060173; P:limb development; IMP:MGI.
DR   GO; GO:0048147; P:negative regulation of fibroblast proliferation; IMP:MGI.
DR   GO; GO:0032968; P:positive regulation of transcription elongation by RNA polymerase II; NAS:ComplexPortal.
DR   GO; GO:0060261; P:positive regulation of transcription initiation by RNA polymerase II; NAS:ComplexPortal.
DR   GO; GO:0016567; P:protein ubiquitination; ISO:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; NAS:ComplexPortal.
DR   Gene3D; 1.10.10.1340; Mediator of RNA polymerase II, submodule Med31 (Soh1); 1.
DR   InterPro; IPR038089; Med31_sf.
DR   InterPro; IPR008831; Mediator_Med31.
DR   PANTHER; PTHR13186; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31; 1.
DR   PANTHER; PTHR13186:SF0; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 31; 1.
DR   Pfam; PF05669; Med31; 1.
DR   Genevisible; Q9CXU1; MM.
PE   1: Evidence at protein level;
KW   3D-structure; Acetylation; Activator; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y3C7"
FT   CHAIN           2..131
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   31"
FT                   /id="PRO_0000212528"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y3C7"
FT   CONFLICT        86
FT                   /note="K -> E (in Ref. 1; BAB29102)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   131 AA;  15771 MW;  A9837A88EC94D014 CRC64;
     MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN YLKYLLYWKE
     PEYAKYLKYP QCLHMLELLQ YEHFRKELVN AQCAKFIDEQ QILHWQHYSR KRVRLQQALA
     EQQQQNNTAG K
//