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Protein

Mediator of RNA polymerase II transcription subunit 31

Gene

Med31

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • limb development Source: MGI
  • negative regulation of fibroblast proliferation Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: GO_Central
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 31
Alternative name(s):
Mediator complex subunit 31
Mediator complex subunit SOH1
Gene namesi
Name:Med31
Synonyms:Soh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914529. Med31.

Subcellular locationi

GO - Cellular componenti

  • core mediator complex Source: GO_Central
  • mediator complex Source: MGI
  • nucleoplasm Source: Reactome
  • ubiquitin ligase complex Source: Ensembl

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002125282 – 131Mediator of RNA polymerase II transcription subunit 31Add BLAST130

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9CXU1.
MaxQBiQ9CXU1.
PaxDbiQ9CXU1.
PeptideAtlasiQ9CXU1.
PRIDEiQ9CXU1.

PTM databases

PhosphoSitePlusiQ9CXU1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020801.
GenevisibleiQ9CXU1. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Med19Q8C1S02EBI-309355,EBI-398761

Protein-protein interaction databases

BioGridi212068. 2 interactors.
IntActiQ9CXU1. 8 interactors.
MINTiMINT-219187.
STRINGi10090.ENSMUSP00000021157.

Structurei

3D structure databases

ProteinModelPortaliQ9CXU1.
SMRiQ9CXU1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi122 – 125Poly-Gln4

Sequence similaritiesi

Belongs to the Mediator complex subunit 31 family.Curated

Phylogenomic databases

eggNOGiKOG4086. Eukaryota.
COG5088. LUCA.
GeneTreeiENSGT00390000015531.
HOGENOMiHOG000242147.
HOVERGENiHBG052449.
InParanoidiQ9CXU1.
KOiK15153.
OMAiQCTKFID.
OrthoDBiEOG091G0VGJ.
PhylomeDBiQ9CXU1.
TreeFamiTF105799.

Family and domain databases

InterProiView protein in InterPro
IPR008831. Mediator_Med31.
PANTHERiPTHR13186. PTHR13186. 1 hit.
PfamiView protein in Pfam
PF05669. Med31. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CXU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAVAMETD DAGNRLRFQL ELEFVQCLAN PNYLNFLAQR GYFKDKAFVN
60 70 80 90 100
YLKYLLYWKE PEYAKYLKYP QCLHMLELLQ YEHFRKELVN AQCAKFIDEQ
110 120 130
QILHWQHYSR KRVRLQQALA EQQQQNNTAG K
Length:131
Mass (Da):15,771
Last modified:October 31, 2003 - v2
Checksum:iA9837A88EC94D014
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86K → E in BAB29102 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005655 mRNA. Translation: BAB24169.1.
AK013990 mRNA. Translation: BAB29102.1.
BX119911 Genomic DNA. Translation: CAI24479.1.
BC019685 mRNA. Translation: AAH19685.1.
BC051929 mRNA. Translation: AAH51929.1.
CCDSiCCDS24981.1.
RefSeqiNP_080344.2. NM_026068.2.
UniGeneiMm.159496.

Genome annotation databases

EnsembliENSMUST00000021157; ENSMUSP00000021157; ENSMUSG00000020801.
GeneIDi67279.
KEGGimmu:67279.
UCSCiuc007jyk.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMED31_MOUSE
AccessioniPrimary (citable) accession number: Q9CXU1
Secondary accession number(s): Q9DAP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: October 31, 2003
Last modified: May 10, 2017
This is version 119 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families