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Q9CXJ1

- SYEM_MOUSE

UniProt

Q9CXJ1 - SYEM_MOUSE

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Protein

Probable glutamate--tRNA ligase, mitochondrial

Gene

Ears2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501ATPBy similarity
Binding sitei76 – 761GlutamateBy similarity
Binding sitei246 – 2461GlutamateBy similarity
Binding sitei249 – 2491ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi284 – 2885ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-tRNA(Gln) ligase activity Source: Ensembl
  3. glutamate-tRNA ligase activity Source: UniProtKB-EC
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: Ensembl
  2. tRNA aminoacylation for mitochondrial protein translation Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, mitochondrial (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:Ears2
Synonyms:Kiaa1970
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1914667. Ears2.

Subcellular locationi

Mitochondrion matrix By similarity

GO - Cellular componenti

  1. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4141MitochondrionSequence AnalysisAdd
BLAST
Chaini42 – 523482Probable glutamate--tRNA ligase, mitochondrialPRO_0000254562Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei256 – 2561N6-succinyllysine1 Publication
Modified residuei486 – 4861N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ9CXJ1.
PaxDbiQ9CXJ1.
PRIDEiQ9CXJ1.

PTM databases

PhosphoSiteiQ9CXJ1.

Expressioni

Gene expression databases

BgeeiQ9CXJ1.
GenevestigatoriQ9CXJ1.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033159.

Structurei

3D structure databases

ProteinModelPortaliQ9CXJ1.
SMRiQ9CXJ1. Positions 37-499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni40 – 423Glutamate bindingBy similarity
Regioni228 – 2325Glutamate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 539"HIGH" region
Motifi284 – 2885"KMSKS" region

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0008.
GeneTreeiENSGT00390000009759.
HOGENOMiHOG000252720.
HOVERGENiHBG056174.
InParanoidiQ9CXJ1.
KOiK01885.
OMAiTKHSNVM.
OrthoDBiEOG73Z2SZ.
PhylomeDBiQ9CXJ1.
TreeFamiTF313268.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CXJ1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAPLKRLLL AEPHVVALGH RVGRREASLG PDPGAPVRVR FAPSPTGFLH
60 70 80 90 100
LGGLRTALYN YIFAKKHQGS FILRLEDTDQ SRLVPGAAES IEDMLEWAGI
110 120 130 140 150
PPDESPRRGG PAGPYCQSQR LALYAQATEA LLRSGAAYPC FCLPQRLELL
160 170 180 190 200
KKEALRSRQT PRYDNRCRNL SQAQVAQKLA VDPKPAIRFR LEEAVPAFQD
210 220 230 240 250
LVYGWTQHEV ASVEGDPVIL KSDGFPTYHL ACVVDDHHMS ISHVLRGSEW
260 270 280 290 300
LVSTSKHLLL YQALGWQPPR FAHLPLLLNR DGSKLSKRQG DIFLEHFAAT
310 320 330 340 350
GFLPEALLDI ITNCGSGFAE NQMGRTLPEL ITQFDLTRIT CHSALLDLEK
360 370 380 390 400
LPEFNRLHLR RLVSSETQRP QLVEKLQGLV KEAFGSELQN KDVLDPAYME
410 420 430 440 450
RILLLRQGHI SRLQDLVSPV YSYLWTRPAV HRSELGASSE NVDVIAKRLL
460 470 480 490 500
GLLERPGLSL TQDVLNRELK KLSEGLEGAK HSSVMKLLRM ALSGQLQGPP
510 520
VAEMMVSLGP KEVRERIQKV LSS
Length:523
Mass (Da):58,326
Last modified:June 1, 2001 - v1
Checksum:i39D506771FB96608
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti188 – 1881R → S in BAE39582. (PubMed:16141072)Curated
Sequence conflicti227 – 2271T → A in BAE39582. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK014324 mRNA. Translation: BAB29273.1.
AK167506 mRNA. Translation: BAE39582.1.
AK169225 mRNA. Translation: BAE40994.1.
BC025907 mRNA. Translation: AAH25907.1.
CCDSiCCDS21807.1.
RefSeqiNP_080416.1. NM_026140.2.
UniGeneiMm.45171.

Genome annotation databases

EnsembliENSMUST00000033159; ENSMUSP00000033159; ENSMUSG00000030871.
GeneIDi67417.
KEGGimmu:67417.
UCSCiuc009joe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK014324 mRNA. Translation: BAB29273.1 .
AK167506 mRNA. Translation: BAE39582.1 .
AK169225 mRNA. Translation: BAE40994.1 .
BC025907 mRNA. Translation: AAH25907.1 .
CCDSi CCDS21807.1.
RefSeqi NP_080416.1. NM_026140.2.
UniGenei Mm.45171.

3D structure databases

ProteinModelPortali Q9CXJ1.
SMRi Q9CXJ1. Positions 37-499.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10090.ENSMUSP00000033159.

Chemistry

BindingDBi Q9CXJ1.

PTM databases

PhosphoSitei Q9CXJ1.

Proteomic databases

MaxQBi Q9CXJ1.
PaxDbi Q9CXJ1.
PRIDEi Q9CXJ1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000033159 ; ENSMUSP00000033159 ; ENSMUSG00000030871 .
GeneIDi 67417.
KEGGi mmu:67417.
UCSCi uc009joe.1. mouse.

Organism-specific databases

CTDi 124454.
MGIi MGI:1914667. Ears2.
Rougei Search...

Phylogenomic databases

eggNOGi COG0008.
GeneTreei ENSGT00390000009759.
HOGENOMi HOG000252720.
HOVERGENi HBG056174.
InParanoidi Q9CXJ1.
KOi K01885.
OMAi TKHSNVM.
OrthoDBi EOG73Z2SZ.
PhylomeDBi Q9CXJ1.
TreeFami TF313268.

Miscellaneous databases

ChiTaRSi EARS2. mouse.
NextBioi 324506.
PROi Q9CXJ1.
SOURCEi Search...

Gene expression databases

Bgeei Q9CXJ1.
Genevestigatori Q9CXJ1.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head and Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  3. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-256, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiSYEM_MOUSE
AccessioniPrimary (citable) accession number: Q9CXJ1
Secondary accession number(s): Q3TJB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3