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Protein

Probable glutamate--tRNA ligase, mitochondrial

Gene

Ears2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50ATPBy similarity1
Binding sitei76GlutamateBy similarity1
Binding sitei246GlutamateBy similarity1
Binding sitei249ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi284 – 288ATPBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamate--tRNA ligase, mitochondrial (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:Ears2
Synonyms:Kiaa1970
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1914667. Ears2.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 41MitochondrionSequence analysisAdd BLAST41
ChainiPRO_000025456242 – 523Probable glutamate--tRNA ligase, mitochondrialAdd BLAST482

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei256N6-succinyllysineCombined sources1
Modified residuei486N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9CXJ1.
MaxQBiQ9CXJ1.
PaxDbiQ9CXJ1.
PeptideAtlasiQ9CXJ1.
PRIDEiQ9CXJ1.

PTM databases

iPTMnetiQ9CXJ1.
PhosphoSitePlusiQ9CXJ1.
SwissPalmiQ9CXJ1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030871.
GenevisibleiQ9CXJ1. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033159.

Chemistry databases

BindingDBiQ9CXJ1.

Structurei

3D structure databases

ProteinModelPortaliQ9CXJ1.
SMRiQ9CXJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 42Glutamate bindingBy similarity3
Regioni228 – 232Glutamate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi45 – 53"HIGH" region9
Motifi284 – 288"KMSKS" region5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1149. Eukaryota.
COG0008. LUCA.
GeneTreeiENSGT00390000009759.
HOGENOMiHOG000252720.
HOVERGENiHBG056174.
InParanoidiQ9CXJ1.
KOiK01885.
OMAiPAHSYLW.
OrthoDBiEOG091G08PN.
PhylomeDBiQ9CXJ1.
TreeFamiTF313268.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CXJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAPLKRLLL AEPHVVALGH RVGRREASLG PDPGAPVRVR FAPSPTGFLH
60 70 80 90 100
LGGLRTALYN YIFAKKHQGS FILRLEDTDQ SRLVPGAAES IEDMLEWAGI
110 120 130 140 150
PPDESPRRGG PAGPYCQSQR LALYAQATEA LLRSGAAYPC FCLPQRLELL
160 170 180 190 200
KKEALRSRQT PRYDNRCRNL SQAQVAQKLA VDPKPAIRFR LEEAVPAFQD
210 220 230 240 250
LVYGWTQHEV ASVEGDPVIL KSDGFPTYHL ACVVDDHHMS ISHVLRGSEW
260 270 280 290 300
LVSTSKHLLL YQALGWQPPR FAHLPLLLNR DGSKLSKRQG DIFLEHFAAT
310 320 330 340 350
GFLPEALLDI ITNCGSGFAE NQMGRTLPEL ITQFDLTRIT CHSALLDLEK
360 370 380 390 400
LPEFNRLHLR RLVSSETQRP QLVEKLQGLV KEAFGSELQN KDVLDPAYME
410 420 430 440 450
RILLLRQGHI SRLQDLVSPV YSYLWTRPAV HRSELGASSE NVDVIAKRLL
460 470 480 490 500
GLLERPGLSL TQDVLNRELK KLSEGLEGAK HSSVMKLLRM ALSGQLQGPP
510 520
VAEMMVSLGP KEVRERIQKV LSS
Length:523
Mass (Da):58,326
Last modified:June 1, 2001 - v1
Checksum:i39D506771FB96608
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188R → S in BAE39582 (PubMed:16141072).Curated1
Sequence conflicti227T → A in BAE39582 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014324 mRNA. Translation: BAB29273.1.
AK167506 mRNA. Translation: BAE39582.1.
AK169225 mRNA. Translation: BAE40994.1.
BC025907 mRNA. Translation: AAH25907.1.
CCDSiCCDS21807.1.
RefSeqiNP_080416.1. NM_026140.3.
UniGeneiMm.45171.
Mm.49615.

Genome annotation databases

EnsembliENSMUST00000033159; ENSMUSP00000033159; ENSMUSG00000030871.
GeneIDi67417.
KEGGimmu:67417.
UCSCiuc009joe.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014324 mRNA. Translation: BAB29273.1.
AK167506 mRNA. Translation: BAE39582.1.
AK169225 mRNA. Translation: BAE40994.1.
BC025907 mRNA. Translation: AAH25907.1.
CCDSiCCDS21807.1.
RefSeqiNP_080416.1. NM_026140.3.
UniGeneiMm.45171.
Mm.49615.

3D structure databases

ProteinModelPortaliQ9CXJ1.
SMRiQ9CXJ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033159.

Chemistry databases

BindingDBiQ9CXJ1.

PTM databases

iPTMnetiQ9CXJ1.
PhosphoSitePlusiQ9CXJ1.
SwissPalmiQ9CXJ1.

Proteomic databases

EPDiQ9CXJ1.
MaxQBiQ9CXJ1.
PaxDbiQ9CXJ1.
PeptideAtlasiQ9CXJ1.
PRIDEiQ9CXJ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033159; ENSMUSP00000033159; ENSMUSG00000030871.
GeneIDi67417.
KEGGimmu:67417.
UCSCiuc009joe.2. mouse.

Organism-specific databases

CTDi124454.
MGIiMGI:1914667. Ears2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1149. Eukaryota.
COG0008. LUCA.
GeneTreeiENSGT00390000009759.
HOGENOMiHOG000252720.
HOVERGENiHBG056174.
InParanoidiQ9CXJ1.
KOiK01885.
OMAiPAHSYLW.
OrthoDBiEOG091G08PN.
PhylomeDBiQ9CXJ1.
TreeFamiTF313268.

Miscellaneous databases

ChiTaRSiEars2. mouse.
PROiQ9CXJ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030871.
GenevisibleiQ9CXJ1. MM.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYEM_MOUSE
AccessioniPrimary (citable) accession number: Q9CXJ1
Secondary accession number(s): Q3TJB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.