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Q9CXH7

- SGOL1_MOUSE

UniProt

Q9CXH7 - SGOL1_MOUSE

Protein

Shugoshin-like 1

Gene

Sgol1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity.2 Publications

    GO - Molecular functioni

    1. kinase binding Source: MGI

    GO - Biological processi

    1. attachment of spindle microtubules to kinetochore Source: UniProtKB
    2. centriole-centriole cohesion Source: UniProtKB
    3. chromosome segregation Source: UniProtKB
    4. meiotic chromosome segregation Source: MGI
    5. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Chromosome partition, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Shugoshin-like 1
    Gene namesi
    Name:Sgol1
    Synonyms:Sgo1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1919665. Sgol1.

    Subcellular locationi

    Nucleus By similarity. Chromosomecentromere By similarity. Chromosomecentromerekinetochore 1 Publication. Cytoplasmcytoskeletonspindle pole By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
    Note: Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Localizes to the inner kinetochore from prophase to early metaphase. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Colocalizes with NEK2 and SS18L1 at the kinetochore By similarity.By similarity

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. chromosome, centromeric region Source: UniProtKB
    3. condensed chromosome kinetochore Source: UniProtKB-SubCell
    4. condensed nuclear chromosome, centromeric region Source: MGI
    5. cytoplasm Source: UniProtKB-KW
    6. kinetochore Source: UniProtKB
    7. mitotic cohesin complex Source: Ensembl
    8. spindle pole Source: MGI

    Keywords - Cellular componenti

    Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 517517Shugoshin-like 1PRO_0000055437Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei426 – 4261PhosphoserineBy similarity
    Modified residuei497 – 4971Phosphoserine; by NEK2By similarity

    Post-translational modificationi

    Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.By similarity
    Phosphorylation by NEK2 is essential for chromosome congression in mitosis and for the proper attachment of spindle microtubule to the kinetochore. Phosphorylated by PLK1 and AUKRB By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiQ9CXH7.

    PTM databases

    PhosphoSiteiQ9CXH7.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed in proliferating cells. Moderately expressed in the oocytes.1 Publication

    Gene expression databases

    BgeeiQ9CXH7.
    CleanExiMM_SGOL1.
    GenevestigatoriQ9CXH7.

    Interactioni

    Subunit structurei

    Interacts with PPP2CA (or PPP2CB), PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E, SET, LRRC59, RBM10 (or RBM5), RPL10A, RPL28, RPL7, RPL7A and RPLP1. Interaction with protein phosphatase 2A occurs most probably through direct binding to the regulatory B56 subunits: PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E. Interacts with PPP2R1A and NEK2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi215364. 5 interactions.
    IntActiQ9CXH7. 5 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CXH7.
    SMRiQ9CXH7. Positions 51-96.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 176176Necessary for interaction with PPP2CA and PPP2R1ABy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili1 – 8989Sequence AnalysisAdd
    BLAST
    Coiled coili268 – 29124Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi447 – 4559D-boxBy similarity

    Sequence similaritiesi

    Belongs to the shugoshin family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG47262.
    GeneTreeiENSGT00390000014987.
    HOGENOMiHOG000072678.
    HOVERGENiHBG080777.
    InParanoidiQ9CXH7.
    KOiK11580.
    OMAiCYYLTCQ.
    OrthoDBiEOG7FXZZJ.
    PhylomeDBiQ9CXH7.
    TreeFamiTF334213.

    Family and domain databases

    InterProiIPR011515. Shugoshin_C.
    IPR011516. Shugoshin_N.
    [Graphical view]
    PfamiPF07557. Shugoshin_C. 1 hit.
    PF07558. Shugoshin_N. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9CXH7-1) [UniParc]FASTAAdd to Basket

    Also known as: 1A

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAKERCQKRS FQDTLEDIKN RMKEKRNKNL AGIGKRKSFI VAPGQVPTNT    50
    ATLLRYYQDN NRLLVLALEN EKSKVREAQD VILQLRKECY YLTCQLYALK 100
    EKLTSRQSEE TTQNWKGRPS DVVSSIDNTT RDLSGKSLQQ IAVEETDCPY 150
    QTTEPSPAVT PETQGCDFDS GKVESTDEVL PRTISIRRHL RKDFSNISHS 200
    TTLEDCKASP RVAQSLEVKG SRCREVTVTL HRLENVCLWN KDQISLCSRL 250
    INPAKITETE VILSSKPEQI ESKHKRARKR RAEQRRTKQR CKSKSSLRSK 300
    GNKNKDKQGL PPTTLDGGIG SCDAYDFNLK GTVHPTPFRQ KMNNGCNKET 350
    DSSNSEVSDL ECSTSEDESD DLYLPPSKRL RDYRESERAV TRPRSKRGLQ 400
    YPDGKERKEV LPSTAPTGIP PETQESPRCS LKDVTNILQC PRVKIRKPSL 450
    PPKRREDSPA VALTKRRCST IKSYKEPTLA SKLRRGDPFT DLCFLNSPIF 500
    KQKRGMRCPK RRTKQTQ 517
    Length:517
    Mass (Da):58,966
    Last modified:June 1, 2001 - v1
    Checksum:i75E84B2C47833BCA
    GO
    Isoform 2 (identifier: Q9CXH7-2) [UniParc]FASTAAdd to Basket

    Also known as: 1B

    The sequence of this isoform differs from the canonical sequence as follows:
         171-172: GK → ES
         173-517: Missing.

    Show »
    Length:172
    Mass (Da):19,672
    Checksum:iA89379562D76991F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti134 – 1341S → P in BAE32427. (PubMed:16141072)Curated
    Sequence conflicti386 – 3861S → T in BAE32427. (PubMed:16141072)Curated
    Sequence conflicti462 – 4621A → S in BAE32427. (PubMed:16141072)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei171 – 1722GK → ES in isoform 2. 1 PublicationVSP_016796
    Alternative sequencei173 – 517345Missing in isoform 2. 1 PublicationVSP_016797Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB193067 mRNA. Translation: BAD95540.1.
    AB193068 mRNA. Translation: BAD95541.1.
    AK014357 mRNA. Translation: BAB29295.1.
    AK049517 mRNA. Translation: BAC33789.1.
    AK154188 mRNA. Translation: BAE32427.1.
    BC089014 mRNA. Translation: AAH89014.1.
    CCDSiCCDS28881.1. [Q9CXH7-1]
    RefSeqiNP_082508.1. NM_028232.2. [Q9CXH7-1]
    XP_006525032.1. XM_006524969.1. [Q9CXH7-2]
    UniGeneiMm.153202.

    Genome annotation databases

    EnsembliENSMUST00000024736; ENSMUSP00000024736; ENSMUSG00000023940. [Q9CXH7-1]
    GeneIDi72415.
    KEGGimmu:72415.
    UCSCiuc008czq.2. mouse. [Q9CXH7-1]
    uc008czr.2. mouse. [Q9CXH7-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB193067 mRNA. Translation: BAD95540.1 .
    AB193068 mRNA. Translation: BAD95541.1 .
    AK014357 mRNA. Translation: BAB29295.1 .
    AK049517 mRNA. Translation: BAC33789.1 .
    AK154188 mRNA. Translation: BAE32427.1 .
    BC089014 mRNA. Translation: AAH89014.1 .
    CCDSi CCDS28881.1. [Q9CXH7-1 ]
    RefSeqi NP_082508.1. NM_028232.2. [Q9CXH7-1 ]
    XP_006525032.1. XM_006524969.1. [Q9CXH7-2 ]
    UniGenei Mm.153202.

    3D structure databases

    ProteinModelPortali Q9CXH7.
    SMRi Q9CXH7. Positions 51-96.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 215364. 5 interactions.
    IntActi Q9CXH7. 5 interactions.

    PTM databases

    PhosphoSitei Q9CXH7.

    Proteomic databases

    PRIDEi Q9CXH7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024736 ; ENSMUSP00000024736 ; ENSMUSG00000023940 . [Q9CXH7-1 ]
    GeneIDi 72415.
    KEGGi mmu:72415.
    UCSCi uc008czq.2. mouse. [Q9CXH7-1 ]
    uc008czr.2. mouse. [Q9CXH7-2 ]

    Organism-specific databases

    CTDi 151648.
    MGIi MGI:1919665. Sgol1.

    Phylogenomic databases

    eggNOGi NOG47262.
    GeneTreei ENSGT00390000014987.
    HOGENOMi HOG000072678.
    HOVERGENi HBG080777.
    InParanoidi Q9CXH7.
    KOi K11580.
    OMAi CYYLTCQ.
    OrthoDBi EOG7FXZZJ.
    PhylomeDBi Q9CXH7.
    TreeFami TF334213.

    Enzyme and pathway databases

    Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
    REACT_207679. Separation of Sister Chromatids.

    Miscellaneous databases

    NextBioi 336218.
    PROi Q9CXH7.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9CXH7.
    CleanExi MM_SGOL1.
    Genevestigatori Q9CXH7.

    Family and domain databases

    InterProi IPR011515. Shugoshin_C.
    IPR011516. Shugoshin_N.
    [Graphical view ]
    Pfami PF07557. Shugoshin_C. 1 hit.
    PF07558. Shugoshin_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells."
      McGuinness B.E., Hirota T., Kudo N.R., Peters J.-M., Nasmyth K.
      PLoS Biol. 3:433-449(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
      Tissue: Testis.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J and NOD.
      Tissue: Head.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Eye.
    4. "sSgo1, a major splice variant of Sgo1, functions in centriole cohesion where it is regulated by Plk1."
      Wang X., Yang Y., Duan Q., Jiang N., Huang Y., Darzynkiewicz Z., Dai W.
      Dev. Cell 14:331-341(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    5. "Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells."
      Lee J., Kitajima T.S., Tanno Y., Yoshida K., Morita T., Miyano T., Miyake M., Watanabe Y.
      Nat. Cell Biol. 10:42-52(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiSGOL1_MOUSE
    AccessioniPrimary (citable) accession number: Q9CXH7
    Secondary accession number(s): Q3U4K4, Q588H1, Q8BKW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 10, 2006
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Shugoshin is Japanese for guardian spirit (as it is known to be a protector of centromeric cohesin).

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3