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Q9CXH7 (SGOL1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Shugoshin-like 1
Gene names
Name:Sgol1
Synonyms:Sgo1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length517 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Ref.4 Ref.5

Subunit structure

Interacts with PPP2CA (or PPP2CB), PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E, SET, LRRC59, RBM10 (or RBM5), RPL10A, RPL28, RPL7, RPL7A and RPLP1. Interaction with protein phosphatase 2A occurs most probably through direct binding to the regulatory B56 subunits: PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E. Interacts with PPP2R1A and NEK2 By similarity.

Subcellular location

Nucleus By similarity. Chromosomecentromere By similarity. Chromosomecentromerekinetochore. Cytoplasmcytoskeletonspindle pole By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Note: Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Localizes to the inner kinetochore from prophase to early metaphase. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Colocalizes with NEK2 and SS18L1 at the kinetochore By similarity. Ref.5

Tissue specificity

Ubiquitously expressed in proliferating cells. Moderately expressed in the oocytes. Ref.5

Post-translational modification

Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1 By similarity.

Phosphorylation by NEK2 is essential for chromosome congression in mitosis and for the proper attachment of spindle microtubule to the kinetochore. Phosphorylated by PLK1 and AUKRB By similarity.

Miscellaneous

Shugoshin is Japanese for guardian spirit (as it is known to be a protector of centromeric cohesin).

Sequence similarities

Belongs to the shugoshin family.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Chromosome partition
Mitosis
   Cellular componentCentromere
Chromosome
Cytoplasm
Cytoskeleton
Kinetochore
Nucleus
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMPhosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processattachment of spindle microtubules to kinetochore

Inferred from sequence or structural similarity. Source: UniProtKB

centriole-centriole cohesion

Inferred from sequence or structural similarity. Source: UniProtKB

chromosome segregation

Inferred from sequence or structural similarity. Source: UniProtKB

meiotic chromosome segregation

Inferred from sequence orthology Ref.4. Source: MGI

mitotic nuclear division

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcentrosome

Inferred from sequence or structural similarity. Source: UniProtKB

chromosome, centromeric region

Inferred from direct assay Ref.5. Source: UniProtKB

condensed chromosome kinetochore

Inferred from electronic annotation. Source: UniProtKB-SubCell

condensed nuclear chromosome, centromeric region

Inferred from direct assay Ref.5. Source: MGI

cytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

kinetochore

Inferred from direct assay Ref.5. Source: UniProtKB

mitotic cohesin complex

Inferred from electronic annotation. Source: Ensembl

spindle pole

Inferred from sequence orthology Ref.4. Source: MGI

   Molecular_functionkinase binding

Inferred from sequence orthology Ref.4. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9CXH7-1)

Also known as: 1A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9CXH7-2)

Also known as: 1B;

The sequence of this isoform differs from the canonical sequence as follows:
     171-172: GK → ES
     173-517: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 517517Shugoshin-like 1
PRO_0000055437

Regions

Region1 – 176176Necessary for interaction with PPP2CA and PPP2R1A By similarity
Coiled coil1 – 8989 Potential
Coiled coil268 – 29124 Potential
Motif447 – 4559D-box By similarity

Amino acid modifications

Modified residue4261Phosphoserine By similarity
Modified residue4971Phosphoserine; by NEK2 By similarity

Natural variations

Alternative sequence171 – 1722GK → ES in isoform 2.
VSP_016796
Alternative sequence173 – 517345Missing in isoform 2.
VSP_016797

Experimental info

Sequence conflict1341S → P in BAE32427. Ref.2
Sequence conflict3861S → T in BAE32427. Ref.2
Sequence conflict4621A → S in BAE32427. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (1A) [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 75E84B2C47833BCA

FASTA51758,966
        10         20         30         40         50         60 
MAKERCQKRS FQDTLEDIKN RMKEKRNKNL AGIGKRKSFI VAPGQVPTNT ATLLRYYQDN 

        70         80         90        100        110        120 
NRLLVLALEN EKSKVREAQD VILQLRKECY YLTCQLYALK EKLTSRQSEE TTQNWKGRPS 

       130        140        150        160        170        180 
DVVSSIDNTT RDLSGKSLQQ IAVEETDCPY QTTEPSPAVT PETQGCDFDS GKVESTDEVL 

       190        200        210        220        230        240 
PRTISIRRHL RKDFSNISHS TTLEDCKASP RVAQSLEVKG SRCREVTVTL HRLENVCLWN 

       250        260        270        280        290        300 
KDQISLCSRL INPAKITETE VILSSKPEQI ESKHKRARKR RAEQRRTKQR CKSKSSLRSK 

       310        320        330        340        350        360 
GNKNKDKQGL PPTTLDGGIG SCDAYDFNLK GTVHPTPFRQ KMNNGCNKET DSSNSEVSDL 

       370        380        390        400        410        420 
ECSTSEDESD DLYLPPSKRL RDYRESERAV TRPRSKRGLQ YPDGKERKEV LPSTAPTGIP 

       430        440        450        460        470        480 
PETQESPRCS LKDVTNILQC PRVKIRKPSL PPKRREDSPA VALTKRRCST IKSYKEPTLA 

       490        500        510 
SKLRRGDPFT DLCFLNSPIF KQKRGMRCPK RRTKQTQ 

« Hide

Isoform 2 (1B) [UniParc].

Checksum: A89379562D76991F
Show »

FASTA17219,672

References

« Hide 'large scale' references
[1]"Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells."
McGuinness B.E., Hirota T., Kudo N.R., Peters J.-M., Nasmyth K.
PLoS Biol. 3:433-449(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
Tissue: Testis.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J and NOD.
Tissue: Head.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Eye.
[4]"sSgo1, a major splice variant of Sgo1, functions in centriole cohesion where it is regulated by Plk1."
Wang X., Yang Y., Duan Q., Jiang N., Huang Y., Darzynkiewicz Z., Dai W.
Dev. Cell 14:331-341(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells."
Lee J., Kitajima T.S., Tanno Y., Yoshida K., Morita T., Miyano T., Miyake M., Watanabe Y.
Nat. Cell Biol. 10:42-52(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB193067 mRNA. Translation: BAD95540.1.
AB193068 mRNA. Translation: BAD95541.1.
AK014357 mRNA. Translation: BAB29295.1.
AK049517 mRNA. Translation: BAC33789.1.
AK154188 mRNA. Translation: BAE32427.1.
BC089014 mRNA. Translation: AAH89014.1.
CCDSCCDS28881.1. [Q9CXH7-1]
RefSeqNP_082508.1. NM_028232.2. [Q9CXH7-1]
XP_006525032.1. XM_006524969.1. [Q9CXH7-2]
UniGeneMm.153202.

3D structure databases

ProteinModelPortalQ9CXH7.
SMRQ9CXH7. Positions 51-96.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid215364. 5 interactions.
IntActQ9CXH7. 5 interactions.

PTM databases

PhosphoSiteQ9CXH7.

Proteomic databases

PRIDEQ9CXH7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000024736; ENSMUSP00000024736; ENSMUSG00000023940. [Q9CXH7-1]
GeneID72415.
KEGGmmu:72415.
UCSCuc008czq.2. mouse. [Q9CXH7-1]
uc008czr.2. mouse. [Q9CXH7-2]

Organism-specific databases

CTD151648.
MGIMGI:1919665. Sgol1.

Phylogenomic databases

eggNOGNOG47262.
GeneTreeENSGT00390000014987.
HOGENOMHOG000072678.
HOVERGENHBG080777.
InParanoidQ9CXH7.
KOK11580.
OMACYYLTCQ.
OrthoDBEOG7FXZZJ.
PhylomeDBQ9CXH7.
TreeFamTF334213.

Gene expression databases

BgeeQ9CXH7.
CleanExMM_SGOL1.
GenevestigatorQ9CXH7.

Family and domain databases

InterProIPR011515. Shugoshin_C.
IPR011516. Shugoshin_N.
[Graphical view]
PfamPF07557. Shugoshin_C. 1 hit.
PF07558. Shugoshin_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio336218.
PROQ9CXH7.
SOURCESearch...

Entry information

Entry nameSGOL1_MOUSE
AccessionPrimary (citable) accession number: Q9CXH7
Secondary accession number(s): Q3U4K4, Q588H1, Q8BKW2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot