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Q9CXH7

- SGOL1_MOUSE

UniProt

Q9CXH7 - SGOL1_MOUSE

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Protein

Shugoshin-like 1

Gene

Sgol1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity.2 Publications

GO - Molecular functioni

  1. kinase binding Source: MGI

GO - Biological processi

  1. attachment of spindle microtubules to kinetochore Source: UniProtKB
  2. centriole-centriole cohesion Source: UniProtKB
  3. chromosome segregation Source: UniProtKB
  4. meiotic chromosome segregation Source: MGI
  5. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

ReactomeiREACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.
REACT_256756. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Shugoshin-like 1
Gene namesi
Name:Sgol1
Synonyms:Sgo1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1919665. Sgol1.

Subcellular locationi

Nucleus By similarity. Chromosomecentromere By similarity. Chromosomecentromerekinetochore 1 Publication. Cytoplasmcytoskeletonspindle pole By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
Note: Localizes to the inner centromere throughout prophase until metaphase and disappears at anaphase. During prometaphase, it localizes to a single focus, while at metaphase, it localizes to 2 spots corresponding to the 2 centromeres. Centromeric localization requires the presence of BUB1 and the interaction with PPP2R1A. Localizes to the inner kinetochore from prophase to early metaphase. Phosphorylation by AUKRB and the presence of BUB1 are required for localization to the kinetochore. Colocalizes with NEK2 and SS18L1 at the kinetochore (By similarity).By similarity

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. chromosome, centromeric region Source: UniProtKB
  3. condensed nuclear chromosome, centromeric region Source: MGI
  4. cytoplasm Source: UniProtKB-KW
  5. kinetochore Source: UniProtKB
  6. mitotic cohesin complex Source: Ensembl
  7. spindle pole Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 517517Shugoshin-like 1PRO_0000055437Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei426 – 4261PhosphoserineBy similarity
Modified residuei497 – 4971Phosphoserine; by NEK2By similarity

Post-translational modificationi

Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.By similarity
Phosphorylation by NEK2 is essential for chromosome congression in mitosis and for the proper attachment of spindle microtubule to the kinetochore. Phosphorylated by PLK1 and AUKRB (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9CXH7.
PRIDEiQ9CXH7.

PTM databases

PhosphoSiteiQ9CXH7.

Expressioni

Tissue specificityi

Ubiquitously expressed in proliferating cells. Moderately expressed in the oocytes.1 Publication

Gene expression databases

BgeeiQ9CXH7.
CleanExiMM_SGOL1.
GenevestigatoriQ9CXH7.

Interactioni

Subunit structurei

Interacts with PPP2CA (or PPP2CB), PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E, SET, LRRC59, RBM10 (or RBM5), RPL10A, RPL28, RPL7, RPL7A and RPLP1. Interaction with protein phosphatase 2A occurs most probably through direct binding to the regulatory B56 subunits: PPP2R1B, PPP2R5A, PPP2R5B, PPP2R5C, PPP2R5D, PPP2R5E. Interacts with PPP2R1A and NEK2 (By similarity).By similarity

Protein-protein interaction databases

BioGridi215364. 5 interactions.
IntActiQ9CXH7. 5 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9CXH7.
SMRiQ9CXH7. Positions 51-96.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 176176Necessary for interaction with PPP2CA and PPP2R1ABy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1 – 8989Sequence AnalysisAdd
BLAST
Coiled coili268 – 29124Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi447 – 4559D-boxBy similarity

Sequence similaritiesi

Belongs to the shugoshin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG47262.
GeneTreeiENSGT00390000014987.
HOGENOMiHOG000072678.
HOVERGENiHBG080777.
InParanoidiQ9CXH7.
KOiK11580.
OMAiCYYLTCQ.
OrthoDBiEOG7FXZZJ.
PhylomeDBiQ9CXH7.
TreeFamiTF334213.

Family and domain databases

InterProiIPR011515. Shugoshin_C.
IPR011516. Shugoshin_N.
[Graphical view]
PfamiPF07557. Shugoshin_C. 1 hit.
PF07558. Shugoshin_N. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9CXH7-1) [UniParc]FASTAAdd to Basket

Also known as: 1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKERCQKRS FQDTLEDIKN RMKEKRNKNL AGIGKRKSFI VAPGQVPTNT
60 70 80 90 100
ATLLRYYQDN NRLLVLALEN EKSKVREAQD VILQLRKECY YLTCQLYALK
110 120 130 140 150
EKLTSRQSEE TTQNWKGRPS DVVSSIDNTT RDLSGKSLQQ IAVEETDCPY
160 170 180 190 200
QTTEPSPAVT PETQGCDFDS GKVESTDEVL PRTISIRRHL RKDFSNISHS
210 220 230 240 250
TTLEDCKASP RVAQSLEVKG SRCREVTVTL HRLENVCLWN KDQISLCSRL
260 270 280 290 300
INPAKITETE VILSSKPEQI ESKHKRARKR RAEQRRTKQR CKSKSSLRSK
310 320 330 340 350
GNKNKDKQGL PPTTLDGGIG SCDAYDFNLK GTVHPTPFRQ KMNNGCNKET
360 370 380 390 400
DSSNSEVSDL ECSTSEDESD DLYLPPSKRL RDYRESERAV TRPRSKRGLQ
410 420 430 440 450
YPDGKERKEV LPSTAPTGIP PETQESPRCS LKDVTNILQC PRVKIRKPSL
460 470 480 490 500
PPKRREDSPA VALTKRRCST IKSYKEPTLA SKLRRGDPFT DLCFLNSPIF
510
KQKRGMRCPK RRTKQTQ
Length:517
Mass (Da):58,966
Last modified:June 1, 2001 - v1
Checksum:i75E84B2C47833BCA
GO
Isoform 2 (identifier: Q9CXH7-2) [UniParc]FASTAAdd to Basket

Also known as: 1B

The sequence of this isoform differs from the canonical sequence as follows:
     171-172: GK → ES
     173-517: Missing.

Show »
Length:172
Mass (Da):19,672
Checksum:iA89379562D76991F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti134 – 1341S → P in BAE32427. (PubMed:16141072)Curated
Sequence conflicti386 – 3861S → T in BAE32427. (PubMed:16141072)Curated
Sequence conflicti462 – 4621A → S in BAE32427. (PubMed:16141072)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei171 – 1722GK → ES in isoform 2. 1 PublicationVSP_016796
Alternative sequencei173 – 517345Missing in isoform 2. 1 PublicationVSP_016797Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB193067 mRNA. Translation: BAD95540.1.
AB193068 mRNA. Translation: BAD95541.1.
AK014357 mRNA. Translation: BAB29295.1.
AK049517 mRNA. Translation: BAC33789.1.
AK154188 mRNA. Translation: BAE32427.1.
BC089014 mRNA. Translation: AAH89014.1.
CCDSiCCDS28881.1. [Q9CXH7-1]
RefSeqiNP_082508.1. NM_028232.2. [Q9CXH7-1]
XP_006525032.1. XM_006524969.1. [Q9CXH7-2]
UniGeneiMm.153202.

Genome annotation databases

EnsembliENSMUST00000024736; ENSMUSP00000024736; ENSMUSG00000023940. [Q9CXH7-1]
GeneIDi72415.
KEGGimmu:72415.
UCSCiuc008czq.2. mouse. [Q9CXH7-1]
uc008czr.2. mouse. [Q9CXH7-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB193067 mRNA. Translation: BAD95540.1 .
AB193068 mRNA. Translation: BAD95541.1 .
AK014357 mRNA. Translation: BAB29295.1 .
AK049517 mRNA. Translation: BAC33789.1 .
AK154188 mRNA. Translation: BAE32427.1 .
BC089014 mRNA. Translation: AAH89014.1 .
CCDSi CCDS28881.1. [Q9CXH7-1 ]
RefSeqi NP_082508.1. NM_028232.2. [Q9CXH7-1 ]
XP_006525032.1. XM_006524969.1. [Q9CXH7-2 ]
UniGenei Mm.153202.

3D structure databases

ProteinModelPortali Q9CXH7.
SMRi Q9CXH7. Positions 51-96.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 215364. 5 interactions.
IntActi Q9CXH7. 5 interactions.

PTM databases

PhosphoSitei Q9CXH7.

Proteomic databases

MaxQBi Q9CXH7.
PRIDEi Q9CXH7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000024736 ; ENSMUSP00000024736 ; ENSMUSG00000023940 . [Q9CXH7-1 ]
GeneIDi 72415.
KEGGi mmu:72415.
UCSCi uc008czq.2. mouse. [Q9CXH7-1 ]
uc008czr.2. mouse. [Q9CXH7-2 ]

Organism-specific databases

CTDi 151648.
MGIi MGI:1919665. Sgol1.

Phylogenomic databases

eggNOGi NOG47262.
GeneTreei ENSGT00390000014987.
HOGENOMi HOG000072678.
HOVERGENi HBG080777.
InParanoidi Q9CXH7.
KOi K11580.
OMAi CYYLTCQ.
OrthoDBi EOG7FXZZJ.
PhylomeDBi Q9CXH7.
TreeFami TF334213.

Enzyme and pathway databases

Reactomei REACT_198961. Resolution of Sister Chromatid Cohesion.
REACT_207679. Separation of Sister Chromatids.
REACT_256756. Mitotic Prometaphase.

Miscellaneous databases

NextBioi 336218.
PROi Q9CXH7.
SOURCEi Search...

Gene expression databases

Bgeei Q9CXH7.
CleanExi MM_SGOL1.
Genevestigatori Q9CXH7.

Family and domain databases

InterProi IPR011515. Shugoshin_C.
IPR011516. Shugoshin_N.
[Graphical view ]
Pfami PF07557. Shugoshin_C. 1 hit.
PF07558. Shugoshin_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Shugoshin prevents dissociation of cohesin from centromeres during mitosis in vertebrate cells."
    McGuinness B.E., Hirota T., Kudo N.R., Peters J.-M., Nasmyth K.
    PLoS Biol. 3:433-449(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J and NOD.
    Tissue: Head.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6.
    Tissue: Eye.
  4. "sSgo1, a major splice variant of Sgo1, functions in centriole cohesion where it is regulated by Plk1."
    Wang X., Yang Y., Duan Q., Jiang N., Huang Y., Darzynkiewicz Z., Dai W.
    Dev. Cell 14:331-341(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Unified mode of centromeric protection by shugoshin in mammalian oocytes and somatic cells."
    Lee J., Kitajima T.S., Tanno Y., Yoshida K., Morita T., Miyano T., Miyake M., Watanabe Y.
    Nat. Cell Biol. 10:42-52(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiSGOL1_MOUSE
AccessioniPrimary (citable) accession number: Q9CXH7
Secondary accession number(s): Q3U4K4, Q588H1, Q8BKW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: June 1, 2001
Last modified: November 26, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Shugoshin is Japanese for guardian spirit (as it is known to be a protector of centromeric cohesin).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3