Q9CXB8 (ALPK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-protein kinase 1
EC=2.7.11.-

Alternative name(s):
Lymphocyte alpha-protein kinase

Gene names

Name:	Alpk1
Synonyms:	Kiaa1527, Lak

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1231 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Kinases that recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.
Sequence similarities	Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily. Contains 1 alpha-type protein kinase domain.

Ontologies

Keywords
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1231	1231	Alpha-protein kinase 1		PRO_0000260029
Regions
	Domain	1003 – 1223	221	Alpha-type protein kinase
	Compositional bias	902 – 946	45	Ser-rich
Experimental info
	Sequence conflict	93 – 110	18	AALRA…AASAI → VGRILPPVPLTSPREAFR in BAC30902. Ref.2
	Sequence conflict	209 – 210	2	MW → TD in BAB31195. Ref.2
	Sequence conflict	233	1	K → Q in BAD32469. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9CXB8 [UniParc]. Last modified November 28, 2006. Version 2. Checksum: 94C2C68F6C74241D	FASTA	1,231	136,134
	10 20 30 40 50 60 MNNQDAVASI LHECKQVLDR LLLETPDVST EDKSEDQRCR ASLPSELRTL IQEAEEMKWP 70 80 90 100 110 120 FVPEKWQYKQ AMSPEDKTNL QDVIGAGLQQ LLAALRASIL VQDCAAASAI VFLMDRFLYG 130 140 150 160 170 180 LDVSGKLLQV AKGLHKLKPA TPIAPQVVIR QARVSVNSGK LLKAEYILSS LISNNGATGT 190 200 210 220 230 240 WLYRNESDKV LVQSVCIQIR GQILQKLGMW YEAAELIWAS VIGYLTLPQP DKKGISTSLG 250 260 270 280 290 300 ILADIFVSMS KTDYEKFKKS PKVNLALLKE FDHHLLSAAE ACKLAAAFSA YTPLFVLRAV 310 320 330 340 350 360 NIRGTCLLSY SCSADCPPGM KSVHLCEAKE AFEIGLLTKK DGELVSGKQE LHSFIKAAFG 370 380 390 400 410 420 LTTVHSRLHG ETDAVRAARQ LCSEAVGKLY TFSTSPTSQD REGLSQEIMS LISQVKGHLR 430 440 450 460 470 480 VQSFPNLDVC SYVPESFKCG LDRLILHGHV DFQQILETYS QHHTSVCEVF ESTCGNSKSN 490 500 510 520 530 540 QRDTKSEVCI TTLKTETNTA DTMVATLERV SSQDSRSTAS SKMSKKDQGK LQRERGRSWT 550 560 570 580 590 600 RSKAFRVSLD LDMETETEPP NHSNGGTDVF NKSLRDNSSS CSWGRLSGLS SSTSWEEVNC 610 620 630 640 650 660 AVQDVVRKGS GQEKHPVEAQ SSEAVSEEPK RNRSSRAVFL SSKLRGVSLQ TTGDDNLESS 670 680 690 700 710 720 PSQLHNHTSI LPFNAKDTCL ASGAGLVETA EGSNNTSLQS SHSCGSDSWS LSSSDRFTDV 730 740 750 760 770 780 TTNPSVQEEE PSGIMGDVPE SKYDFKDWHG EKNGGTLTEI CTGPELTFAP SSVDPEGETA 790 800 810 820 830 840 ESTDDGLSPS QVALGCLEGS HSMSTRRTFF PDGSVQNADS AKTGCSVRDQ TVDPDASTVD 850 860 870 880 890 900 EEGQMLDSTE VCSIGQDGAH RPRALRSGQS AEGPKSFVNG SRPSPIFDED FSTTEEGEEL 910 920 930 940 950 960 GSMLKSSQNS SSYSPWWLKS PAFSRSSSDG ESSWSLLNSS RSSFASLAGQ TSQEILEART 970 980 990 1000 1010 1020 LQPDDLEKLL AGVRHDWLLQ RLENTGVLKS NQLQQAHSAL LLKYSKKSEL WTAQETVVYL 1030 1040 1050 1060 1070 1080 GDYLKVKKKG KQRNAFWVHY LHQEETLGRY VGKEYKERKG LRHHFTDVER QMTAQHYVTE 1090 1100 1110 1120 1130 1140 FNKRLYEQKI PTQIFYVPST ILLILEDRTI KGCISVEPYI LGEFVKLSNN TKVVKNEYKA 1150 1160 1170 1180 1190 1200 TEYGLAYGHF SYEFSNHRDV VVDLQGWVTG NGKGLIYLTD PQIHSVDQKD VTTNFGKRGI 1210 1220 1230 FYFFNNQHAS CNEICHRLSL TRPSLEQTSK V

References

[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-209. Strain: C57BL/6J. Tissue: Aorta, Lung and Vein.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 233-1231. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC158308 Genomic DNA. No translation available. AC163391 Genomic DNA. No translation available. AK018401 mRNA. Translation: BAB31195.1. AK041315 mRNA. Translation: BAC30902.1. AK173191 mRNA. Translation: BAD32469.1.
IPI	IPI00110130.
UniGene	Mm.158704.
3D structure databases
ProteinModelPortal	Q9CXB8.
SMR	Q9CXB8. Positions 1049-1220.
ModBase	Search...
PTM databases
PhosphoSite	Q9CXB8.
Proteomic databases
PRIDE	Q9CXB8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000161239; ENSMUSP00000123967; ENSMUSG00000028028.
UCSC	uc008rhj.2. mouse.
Organism-specific databases
MGI	MGI:1918731. Alpk1.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG73232.
GeneTree	ENSGT00530000063613.
HOGENOM	HOG000169279.
HOVERGEN	HBG079770.
InParanoid	Q9CXB8.
OrthoDB	EOG4QNMVF.
Gene expression databases
ArrayExpress	Q9CXB8.
Bgee	Q9CXB8.
CleanEx	MM_ALPK1.
Genevestigator	Q9CXB8.
GermOnline	ENSMUSG00000032755. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR004166. MHCK_EF2_kinase. [Graphical view]
Pfam	PF02816. Alpha_kinase. 1 hit. [Graphical view]
SMART	SM00811. Alpha_kinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51158. ALPHA_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ALPK1. mouse.
SOURCE	Search...

Entry information

Entry name

ALPK1_MOUSE

Accession

Primary (citable) accession number: Q9CXB8
Secondary accession number(s): Q69ZH5, Q8BLT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 28, 2006
Last modified:	May 1, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program