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Protein

GRB2-related adapter protein

Gene

Grap

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Couples signals from receptor and cytoplasmic tyrosine kinases to the Ras signaling pathway.By similarity

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-1433557. Signaling by SCF-KIT.

Names & Taxonomyi

Protein namesi
Recommended name:
GRB2-related adapter protein
Gene namesi
Name:Grap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1918770. Grap.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000882071 – 217GRB2-related adapter proteinAdd BLAST217

Proteomic databases

EPDiQ9CX99.
MaxQBiQ9CX99.
PaxDbiQ9CX99.
PRIDEiQ9CX99.

PTM databases

iPTMnetiQ9CX99.
PhosphoSitePlusiQ9CX99.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004837.
CleanExiMM_GRAP.
GenevisibleiQ9CX99. MM.

Interactioni

Subunit structurei

Associates through its SH2 domain with ligand-activated receptors for stem cell factor (KIT) and erythropoietin (EPOR). Also forms a stable complex with the Bcr-Abl oncoprotein. GRAP is associated with the Ras guanine nucleotide exchange factor SOS1, primarily through its N-terminal SH3 domain. Interacts with phosphorylated LAT upon TCR activation. Interacts with SHB (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9CX99. 4 interactors.
STRINGi10090.ENSMUSP00000004959.

Structurei

3D structure databases

ProteinModelPortaliQ9CX99.
SMRiQ9CX99.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 58SH3 1PROSITE-ProRule annotationAdd BLAST58
Domaini60 – 152SH2PROSITE-ProRule annotationAdd BLAST93
Domaini156 – 215SH3 2PROSITE-ProRule annotationAdd BLAST60

Sequence similaritiesi

Belongs to the GRB2/sem-5/DRK family.Curated

Keywords - Domaini

Repeat, SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG3601. Eukaryota.
ENOG410XR1G. LUCA.
GeneTreeiENSGT00820000126999.
HOGENOMiHOG000251625.
HOVERGENiHBG005404.
InParanoidiQ9CX99.
OMAiRACFAQA.
OrthoDBiEOG091G0HWS.
PhylomeDBiQ9CX99.
TreeFamiTF354288.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiView protein in InterPro
IPR000980. SH2.
IPR011511. SH3_2.
IPR001452. SH3_domain.
PfamiView protein in Pfam
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
PF07653. SH3_2. 1 hit.
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
SMARTiView protein in SMART
SM00252. SH2. 1 hit.
SM00326. SH3. 2 hits.
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF55550. SSF55550. 1 hit.
PROSITEiView protein in PROSITE
PS50001. SH2. 1 hit.
PS50002. SH3. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9CX99-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESVALYSFQ ATESDELAFN KGDTLKILNM EDDQNWYKAE LRGAEGFVPK
60 70 80 90 100
NYIRVKPHPW YSGRISRQLA EETLMKRNHL GAFLIRESES SPGEFSVSVN
110 120 130 140 150
YGDQVQHFKV LREASGKYFL WEEKFNSLNE LVDFYRTTTI AKRRQIFLCD
160 170 180 190 200
EQPLIKPSRA CFAQAQFDFS AQDPSQLSLR RGDIVEVVER EDPHWWRGRA
210
GGRLGFFPRS YVQPVHL
Length:217
Mass (Da):25,277
Last modified:June 1, 2001 - v1
Checksum:i1AF124C3D2773DD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018457 mRNA. Translation: BAB31222.1.
AK153888 mRNA. Translation: BAE32235.1.
AL596209 Genomic DNA. Translation: CAI24871.1.
BC120674 mRNA. Translation: AAI20675.1.
BC120676 mRNA. Translation: AAI20677.1.
CCDSiCCDS24817.1.
RefSeqiNP_082093.1. NM_027817.3.
UniGeneiMm.202760.

Genome annotation databases

EnsembliENSMUST00000004959; ENSMUSP00000004959; ENSMUSG00000004837.
GeneIDi71520.
KEGGimmu:71520.
UCSCiuc007jhz.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGRAP_MOUSE
AccessioniPrimary (citable) accession number: Q9CX99
Secondary accession number(s): Q0VBE3, Q3U545
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: June 1, 2001
Last modified: May 10, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families