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Protein

Cytochrome P450 2U1

Gene

Cyp2u1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the hydroxylation of arachidonic acid, docosahexaenoic acid and other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes (By similarity).By similarity

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi476 – 4761Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: GO_Central
  3. iron ion binding Source: InterPro
  4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  5. oxygen binding Source: GO_Central
  6. steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  1. exogenous drug catabolic process Source: GO_Central
  2. organic acid metabolic process Source: GO_Central
  3. oxidation-reduction process Source: GO_Central
  4. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiREACT_300263. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
REACT_314116. Miscellaneous substrates.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2U1 (EC:1.14.14.1)
Gene namesi
Name:Cyp2u1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1918769. Cyp2u1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei21 – 4121HelicalSequence AnalysisAdd
BLAST
Transmembranei99 – 11921HelicalSequence AnalysisAdd
BLAST
Transmembranei247 – 26721HelicalSequence AnalysisAdd
BLAST
Transmembranei328 – 34821HelicalSequence AnalysisAdd
BLAST
Transmembranei481 – 50121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
  4. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 530530Cytochrome P450 2U1PRO_0000291757Add
BLAST

Proteomic databases

PRIDEiQ9CX98.

PTM databases

PhosphoSiteiQ9CX98.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Developmental stagei

Expressed at all stages after E7.1 Publication

Gene expression databases

BgeeiQ9CX98.
GenevestigatoriQ9CX98.

Structurei

3D structure databases

ProteinModelPortaliQ9CX98.
SMRiQ9CX98. Positions 50-530.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiQ9CX98.
KOiK07422.
OMAiIEEFAYV.
OrthoDBiEOG7RBZ85.
PhylomeDBiQ9CX98.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CX98-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSLGDQRPA AGEQPGARLH VRATGGALLL CLLAVLLGWV WLRRQRACGI
60 70 80 90 100
PPGPKPRPLV GNFGHLLVPR FLRPQFWLGS GSQTDTVGQH VYLARMARVY
110 120 130 140 150
GNIFSFFIGH RLVVVLSDFH SVREALVQQA EVFSDRPRMP LISIMTKEKG
160 170 180 190 200
IVFAHYGPIW KQQRRFSHST LRHFGLGKLS LEPRIIEEFA YVKEAMQKHG
210 220 230 240 250
EAPFSPFPII SNAVSNIICS LCFGQRFDYT NKEFKKVLDF MSRGLEICLH
260 270 280 290 300
SQLFLINICP WFYYLPFGPF KELRQIERDI SCFLKNIIRE HQESLDASNP
310 320 330 340 350
QDFIDMYLLH MEEEQGASRR SSFDEDYLFY IIGDLFIAGT DTTTNSLLWC
360 370 380 390 400
LLYMSLNPDV QKKVHEEIER VIGCDRAPSL TDKAQMPYTE ATIMEVQRLS
410 420 430 440 450
MVVPLAIPHM TSEKTVLQGF TIPKGTVVLI NLWSVHRDPA IWEKPDDFCP
460 470 480 490 500
HRFLDDQGQL LKRETFIPFG IGKRVCMGEQ LAKMELFLMF VSLMQTFTFA
510 520 530
LPEGSEKPVM TGRFGLTLAP HPFNVTISKR
Length:530
Mass (Da):60,578
Last modified:June 25, 2007 - v2
Checksum:iFCB595EADA88A213
GO
Isoform 2 (identifier: Q9CX98-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     473-501: KRVCMGEQLAKMELFLMFVSLMQTFTFAL → QLKLGFNLFFTLSLVCVCVCVCVCVYRHV
     502-530: Missing.

Show »
Length:501
Mass (Da):57,321
Checksum:i4A409AA7E463D369
GO
Isoform 3 (identifier: Q9CX98-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     236-270: Missing.
     416-416: V → A
     417-530: Missing.

Note: No experimental confirmation available.

Show »
Length:381
Mass (Da):43,367
Checksum:iE9832B9102B2DF99
GO

Sequence cautioni

The sequence AK142740 differs from that shown. Reason: Frameshift at position 245. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei236 – 27035Missing in isoform 3. 1 PublicationVSP_026224Add
BLAST
Alternative sequencei416 – 4161V → A in isoform 3. 1 PublicationVSP_026225
Alternative sequencei417 – 530114Missing in isoform 3. 1 PublicationVSP_026226Add
BLAST
Alternative sequencei473 – 50129KRVCM…FTFAL → QLKLGFNLFFTLSLVCVCVC VCVCVYRHV in isoform 2. 1 PublicationVSP_026227Add
BLAST
Alternative sequencei502 – 53029Missing in isoform 2. 1 PublicationVSP_026228Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018458 mRNA. Translation: BAB31223.1.
AK041477 mRNA. Translation: BAC30954.1.
AK142740 mRNA. No translation available.
CCDSiCCDS51069.1. [Q9CX98-1]
RefSeqiNP_082092.2. NM_027816.3. [Q9CX98-1]
UniGeneiMm.473179.

Genome annotation databases

EnsembliENSMUST00000106337; ENSMUSP00000101944; ENSMUSG00000027983. [Q9CX98-1]
GeneIDi71519.
KEGGimmu:71519.
UCSCiuc008rjn.1. mouse. [Q9CX98-1]
uc008rjo.1. mouse. [Q9CX98-2]
uc008rjp.1. mouse. [Q9CX98-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK018458 mRNA. Translation: BAB31223.1.
AK041477 mRNA. Translation: BAC30954.1.
AK142740 mRNA. No translation available.
CCDSiCCDS51069.1. [Q9CX98-1]
RefSeqiNP_082092.2. NM_027816.3. [Q9CX98-1]
UniGeneiMm.473179.

3D structure databases

ProteinModelPortaliQ9CX98.
SMRiQ9CX98. Positions 50-530.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9CX98.

Proteomic databases

PRIDEiQ9CX98.

Protocols and materials databases

DNASUi71519.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106337; ENSMUSP00000101944; ENSMUSG00000027983. [Q9CX98-1]
GeneIDi71519.
KEGGimmu:71519.
UCSCiuc008rjn.1. mouse. [Q9CX98-1]
uc008rjo.1. mouse. [Q9CX98-2]
uc008rjp.1. mouse. [Q9CX98-3]

Organism-specific databases

CTDi113612.
MGIiMGI:1918769. Cyp2u1.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiQ9CX98.
KOiK07422.
OMAiIEEFAYV.
OrthoDBiEOG7RBZ85.
PhylomeDBiQ9CX98.
TreeFamiTF352043.

Enzyme and pathway databases

ReactomeiREACT_300263. Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE).
REACT_314116. Miscellaneous substrates.

Miscellaneous databases

NextBioi333927.
PROiQ9CX98.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CX98.
GenevestigatoriQ9CX98.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J.
    Tissue: Heart, Lung and Thymus.
  2. "Expression patterns of mouse and human CYP orthologs (families 1-4) during development and in different adult tissues."
    Choudhary D., Jansson I., Stoilov I., Sarfarazi M., Schenkman J.B.
    Arch. Biochem. Biophys. 436:50-61(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiCP2U1_MOUSE
AccessioniPrimary (citable) accession number: Q9CX98
Secondary accession number(s): Q8BIM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 25, 2007
Last sequence update: June 25, 2007
Last modified: March 31, 2015
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.