Q9CX98 (CP2U1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2U1
EC=1.14.14.1

Gene names

Name:

Cyp2u1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	530 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the hydroxylation of arachidonic acid, docosahexaenoic acid and other long chain fatty acids. May modulate the arachidonic acid signaling pathway and play a role in other fatty acid signaling processes By similarity.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity. Microsome membrane By similarity.
Tissue specificity	Widely expressed. Ref.2
Developmental stage	Expressed at all stages after E7. Ref.2
Sequence similarities	Belongs to the cytochrome P450 family.
Sequence caution	The sequence AK142740 differs from that shown. Reason: Frameshift at position 245.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9CX98-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9CX98-2)

The sequence of this isoform differs from the canonical sequence as follows:
     473-501: KRVCMGEQLAKMELFLMFVSLMQTFTFAL → QLKLGFNLFFTLSLVCVCVCVCVCVYRHV
     502-530: Missing.

Isoform 3 (identifier: Q9CX98-3)

The sequence of this isoform differs from the canonical sequence as follows:
     236-270: Missing.
     416-416: V → A
     417-530: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 530	530	Cytochrome P450 2U1		PRO_0000291757
Regions
	Transmembrane	21 – 41	21	Helical; Potential
	Transmembrane	99 – 119	21	Helical; Potential
	Transmembrane	247 – 267	21	Helical; Potential
	Transmembrane	328 – 348	21	Helical; Potential
	Transmembrane	481 – 501	21	Helical; Potential
Sites
	Metal binding	476	1	Iron (heme axial ligand) By similarity
Natural variations
	Alternative sequence	236 – 270	35	Missing in isoform 3.		VSP_026224
	Alternative sequence	416	1	V → A in isoform 3.		VSP_026225
	Alternative sequence	417 – 530	114	Missing in isoform 3.		VSP_026226
	Alternative sequence	473 – 501	29	KRVCM…FTFAL → QLKLGFNLFFTLSLVCVCVC VCVCVYRHV in isoform 2.		VSP_026227
	Alternative sequence	502 – 530	29	Missing in isoform 2.		VSP_026228

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified June 26, 2007. Version 2. Checksum: FCB595EADA88A213	FASTA	530	60,578
	10 20 30 40 50 60 MSSLGDQRPA AGEQPGARLH VRATGGALLL CLLAVLLGWV WLRRQRACGI PPGPKPRPLV 70 80 90 100 110 120 GNFGHLLVPR FLRPQFWLGS GSQTDTVGQH VYLARMARVY GNIFSFFIGH RLVVVLSDFH 130 140 150 160 170 180 SVREALVQQA EVFSDRPRMP LISIMTKEKG IVFAHYGPIW KQQRRFSHST LRHFGLGKLS 190 200 210 220 230 240 LEPRIIEEFA YVKEAMQKHG EAPFSPFPII SNAVSNIICS LCFGQRFDYT NKEFKKVLDF 250 260 270 280 290 300 MSRGLEICLH SQLFLINICP WFYYLPFGPF KELRQIERDI SCFLKNIIRE HQESLDASNP 310 320 330 340 350 360 QDFIDMYLLH MEEEQGASRR SSFDEDYLFY IIGDLFIAGT DTTTNSLLWC LLYMSLNPDV 370 380 390 400 410 420 QKKVHEEIER VIGCDRAPSL TDKAQMPYTE ATIMEVQRLS MVVPLAIPHM TSEKTVLQGF 430 440 450 460 470 480 TIPKGTVVLI NLWSVHRDPA IWEKPDDFCP HRFLDDQGQL LKRETFIPFG IGKRVCMGEQ 490 500 510 520 530 LAKMELFLMF VSLMQTFTFA LPEGSEKPVM TGRFGLTLAP HPFNVTISKR
	Isoform 2 [UniParc]. Checksum: 4A409AA7E463D369	FASTA	501	57,321
	10 20 30 40 50 60 MSSLGDQRPA AGEQPGARLH VRATGGALLL CLLAVLLGWV WLRRQRACGI PPGPKPRPLV 70 80 90 100 110 120 GNFGHLLVPR FLRPQFWLGS GSQTDTVGQH VYLARMARVY GNIFSFFIGH RLVVVLSDFH 130 140 150 160 170 180 SVREALVQQA EVFSDRPRMP LISIMTKEKG IVFAHYGPIW KQQRRFSHST LRHFGLGKLS 190 200 210 220 230 240 LEPRIIEEFA YVKEAMQKHG EAPFSPFPII SNAVSNIICS LCFGQRFDYT NKEFKKVLDF 250 260 270 280 290 300 MSRGLEICLH SQLFLINICP WFYYLPFGPF KELRQIERDI SCFLKNIIRE HQESLDASNP 310 320 330 340 350 360 QDFIDMYLLH MEEEQGASRR SSFDEDYLFY IIGDLFIAGT DTTTNSLLWC LLYMSLNPDV 370 380 390 400 410 420 QKKVHEEIER VIGCDRAPSL TDKAQMPYTE ATIMEVQRLS MVVPLAIPHM TSEKTVLQGF 430 440 450 460 470 480 TIPKGTVVLI NLWSVHRDPA IWEKPDDFCP HRFLDDQGQL LKRETFIPFG IGQLKLGFNL 490 500 FFTLSLVCVC VCVCVCVYRH V
	Isoform 3 [UniParc]. Checksum: E9832B9102B2DF99	FASTA	381	43,367
	10 20 30 40 50 60 MSSLGDQRPA AGEQPGARLH VRATGGALLL CLLAVLLGWV WLRRQRACGI PPGPKPRPLV 70 80 90 100 110 120 GNFGHLLVPR FLRPQFWLGS GSQTDTVGQH VYLARMARVY GNIFSFFIGH RLVVVLSDFH 130 140 150 160 170 180 SVREALVQQA EVFSDRPRMP LISIMTKEKG IVFAHYGPIW KQQRRFSHST LRHFGLGKLS 190 200 210 220 230 240 LEPRIIEEFA YVKEAMQKHG EAPFSPFPII SNAVSNIICS LCFGQRFDYT NKEFKKELRQ 250 260 270 280 290 300 IERDISCFLK NIIREHQESL DASNPQDFID MYLLHMEEEQ GASRRSSFDE DYLFYIIGDL 310 320 330 340 350 360 FIAGTDTTTN SLLWCLLYMS LNPDVQKKVH EEIERVIGCD RAPSLTDKAQ MPYTEATIME 370 380 VQRLSMVVPL AIPHMTSEKT A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3). Strain: C57BL/6J. Tissue: Heart, Lung and Thymus.
[2]	"Expression patterns of mouse and human CYP orthologs (families 1-4) during development and in different adult tissues." Choudhary D., Jansson I., Stoilov I., Sarfarazi M., Schenkman J.B. Arch. Biochem. Biophys. 436:50-61(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK018458 mRNA. Translation: BAB31223.1. AK041477 mRNA. Translation: BAC30954.1. AK142740 mRNA. No translation available.
IPI	IPI00109875. IPI00775884. IPI00850984.
RefSeq	NP_082092.2. NM_027816.3.
UniGene	Mm.473179.
3D structure databases
HSSP	HSSP built from PDB template 2F9Q based on UniProtKB P10635.
ProteinModelPortal	Q9CX98.
SMR	Q9CX98. Positions 50-530.
ModBase	Search...
PTM databases
PhosphoSite	Q9CX98.
Proteomic databases
PRIDE	Q9CX98.
Protocols and materials databases
DNASU	71519.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000106337; ENSMUSP00000101944; ENSMUSG00000027983.
GeneID	71519.
KEGG	mmu:71519.
UCSC	uc008rjn.1. mouse. uc008rjo.1. mouse. uc008rjp.1. mouse.
Organism-specific databases
CTD	113612.
MGI	MGI:1918769. Cyp2u1.
Phylogenomic databases
eggNOG	COG2124.
GeneTree	ENSGT00700000104455.
HOGENOM	HOG000036991.
HOVERGEN	HBG015789.
InParanoid	Q9CX98.
KO	K07422.
OMA	IEEFAYV.
OrthoDB	EOG476K02.
Gene expression databases
Bgee	Q9CX98.
Genevestigator	Q9CX98.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008069. Cyt_P450_E_grp-I_CYP2D-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01686. EP450ICYP2D. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CYP2U1. mouse.
NextBio	333927.
SOURCE	Search...

Entry information

Entry name

CP2U1_MOUSE

Accession

Primary (citable) accession number: Q9CX98
Secondary accession number(s): Q8BIM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 26, 2007
Last sequence update:	June 26, 2007
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program