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Protein

26S proteasome non-ATPase regulatory subunit 8

Gene

Psmd8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.By similarity

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-MMU-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-MMU-350562. Regulation of ornithine decarboxylase (ODC).
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-446652. Interleukin-1 signaling.
R-MMU-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-MMU-4608870. Asymmetric localization of PCP proteins.
R-MMU-4641257. Degradation of AXIN.
R-MMU-4641258. Degradation of DVL.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.
R-MMU-5610780. Degradation of GLI1 by the proteasome.
R-MMU-5610785. GLI3 is processed to GLI3R by the proteasome.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676590. NIK-->noncanonical NF-kB signaling.
R-MMU-5687128. MAPK6/MAPK4 signaling.
R-MMU-5689603. UCH proteinases.
R-MMU-5689880. Ub-specific processing proteases.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-MMU-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-MMU-69481. G2/M Checkpoints.
R-MMU-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-MMU-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-MMU-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-MMU-8941858. Regulation of RUNX3 expression and activity.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 8
Alternative name(s):
26S proteasome regulatory subunit RPN12
26S proteasome regulatory subunit S14
Gene namesi
Name:Psmd8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1888669. Psmd8.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738471 – 35326S proteasome non-ATPase regulatory subunit 8Add BLAST353

Proteomic databases

EPDiQ9CX56.
MaxQBiQ9CX56.
PaxDbiQ9CX56.
PRIDEiQ9CX56.

PTM databases

iPTMnetiQ9CX56.
PhosphoSitePlusiQ9CX56.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030591.
ExpressionAtlasiQ9CX56. baseline and differential.
GenevisibleiQ9CX56. MM.

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD8, a base containing 6 ATPases and few additional components.By similarity

Protein-protein interaction databases

IntActiQ9CX56. 2 interactors.
MINTiMINT-1869975.
STRINGi10090.ENSMUSP00000051657.

Structurei

3D structure databases

ProteinModelPortaliQ9CX56.
SMRiQ9CX56.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the proteasome subunit S14 family.Curated

Phylogenomic databases

eggNOGiKOG3151. Eukaryota.
ENOG410XXP2. LUCA.
GeneTreeiENSGT00390000014682.
HOGENOMiHOG000196008.
HOVERGENiHBG024389.
InParanoidiQ9CX56.
KOiK03031.
OMAiGAFWSIR.
OrthoDBiEOG091G0G73.
PhylomeDBiQ9CX56.
TreeFamiTF106233.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR006746. 26S_Psome_Rpn12.
IPR033464. CSN8_PSD8_EIF3K.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR12387. PTHR12387. 1 hit.
PfamiView protein in Pfam
PF10075. CSN8_PSD8_EIF3K. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CX56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIKGRAAKT PRGEPRRSSR GGRKLAVVAP PPVLGSTSRP HFRRESIARR
60 70 80 90 100
RCRKSGRRLA ASRKMAATAA TVNGSTTVSS SGPAATSVGI LQAAAGMYEQ
110 120 130 140 150
LKDEWNRKNP NLSKCGEELG RLKLVLLELN FLPTTGTKLT KQQLILARDI
160 170 180 190 200
LEIGAQWSIL CKDIPSFERY MAQLKCYYFD YKEQLPESAY MHQLLGLNLL
210 220 230 240 250
FLLSQNRVAE FHTELERLPA KDIQTNVYIK HPVSLEQYLM EGSYNKVFLA
260 270 280 290 300
KGNIPAESYT FFIDILLDTI RDEIAGCIEK AYEKILFAEA TRILFFSTPK
310 320 330 340 350
KMTDYAKKRG WVLGPNNYYS FASQQQKPED STIPSTELAK QVIEYARQLE

MIV
Length:353
Mass (Da):39,930
Last modified:October 5, 2010 - v2
Checksum:i45C8836045CDDDF6
GO

Sequence cautioni

The sequence BAB32006 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC164564 Genomic DNA. No translation available.
AK020132 mRNA. Translation: BAB32006.2. Different initiation.
CCDSiCCDS52169.1.
RefSeqiNP_080821.3. NM_026545.3.
UniGeneiMm.273152.

Genome annotation databases

EnsembliENSMUST00000059642; ENSMUSP00000051657; ENSMUSG00000030591.
ENSMUST00000186182; ENSMUSP00000139514; ENSMUSG00000030591.
GeneIDi57296.
KEGGimmu:57296.
UCSCiuc009gbb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC164564 Genomic DNA. No translation available.
AK020132 mRNA. Translation: BAB32006.2. Different initiation.
CCDSiCCDS52169.1.
RefSeqiNP_080821.3. NM_026545.3.
UniGeneiMm.273152.

3D structure databases

ProteinModelPortaliQ9CX56.
SMRiQ9CX56.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CX56. 2 interactors.
MINTiMINT-1869975.
STRINGi10090.ENSMUSP00000051657.

PTM databases

iPTMnetiQ9CX56.
PhosphoSitePlusiQ9CX56.

Proteomic databases

EPDiQ9CX56.
MaxQBiQ9CX56.
PaxDbiQ9CX56.
PRIDEiQ9CX56.

Protocols and materials databases

DNASUi57296.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059642; ENSMUSP00000051657; ENSMUSG00000030591.
ENSMUST00000186182; ENSMUSP00000139514; ENSMUSG00000030591.
GeneIDi57296.
KEGGimmu:57296.
UCSCiuc009gbb.2. mouse.

Organism-specific databases

CTDi5714.
MGIiMGI:1888669. Psmd8.

Phylogenomic databases

eggNOGiKOG3151. Eukaryota.
ENOG410XXP2. LUCA.
GeneTreeiENSGT00390000014682.
HOGENOMiHOG000196008.
HOVERGENiHBG024389.
InParanoidiQ9CX56.
KOiK03031.
OMAiGAFWSIR.
OrthoDBiEOG091G0G73.
PhylomeDBiQ9CX56.
TreeFamiTF106233.

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-MMU-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-MMU-350562. Regulation of ornithine decarboxylase (ODC).
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-446652. Interleukin-1 signaling.
R-MMU-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-MMU-4608870. Asymmetric localization of PCP proteins.
R-MMU-4641257. Degradation of AXIN.
R-MMU-4641258. Degradation of DVL.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.
R-MMU-5610780. Degradation of GLI1 by the proteasome.
R-MMU-5610785. GLI3 is processed to GLI3R by the proteasome.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676590. NIK-->noncanonical NF-kB signaling.
R-MMU-5687128. MAPK6/MAPK4 signaling.
R-MMU-5689603. UCH proteinases.
R-MMU-5689880. Ub-specific processing proteases.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-MMU-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-MMU-69481. G2/M Checkpoints.
R-MMU-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-MMU-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-MMU-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-MMU-8941858. Regulation of RUNX3 expression and activity.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiPR:Q9CX56.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030591.
ExpressionAtlasiQ9CX56. baseline and differential.
GenevisibleiQ9CX56. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR006746. 26S_Psome_Rpn12.
IPR033464. CSN8_PSD8_EIF3K.
IPR011991. WHTH_DNA-bd_dom.
PANTHERiPTHR12387. PTHR12387. 1 hit.
PfamiView protein in Pfam
PF10075. CSN8_PSD8_EIF3K. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPSMD8_MOUSE
AccessioniPrimary (citable) accession number: Q9CX56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: October 5, 2010
Last modified: June 7, 2017
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-65 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.