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Protein

26S proteasome non-ATPase regulatory subunit 8

Gene

Psmd8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.By similarity

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-MMU-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-MMU-350562. Regulation of ornithine decarboxylase (ODC).
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-446652. Interleukin-1 family signaling.
R-MMU-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-MMU-4608870. Asymmetric localization of PCP proteins.
R-MMU-4641257. Degradation of AXIN.
R-MMU-4641258. Degradation of DVL.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.
R-MMU-5610780. Degradation of GLI1 by the proteasome.
R-MMU-5610785. GLI3 is processed to GLI3R by the proteasome.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676590. NIK-->noncanonical NF-kB signaling.
R-MMU-5687128. MAPK6/MAPK4 signaling.
R-MMU-5689603. UCH proteinases.
R-MMU-5689880. Ub-specific processing proteases.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-MMU-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-MMU-69481. G2/M Checkpoints.
R-MMU-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-MMU-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-MMU-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-MMU-8939236. RUNX1 regulates transcription of genes involved in differentiation of HSCs.
R-MMU-8939902. Regulation of RUNX2 expression and activity.
R-MMU-8941858. Regulation of RUNX3 expression and activity.
R-MMU-8948751. Regulation of PTEN stability and activity.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome non-ATPase regulatory subunit 8
Alternative name(s):
26S proteasome regulatory subunit RPN12
26S proteasome regulatory subunit S14
Gene namesi
Name:Psmd8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1888669. Psmd8.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001738471 – 35326S proteasome non-ATPase regulatory subunit 8Add BLAST353

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki300Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

EPDiQ9CX56.
MaxQBiQ9CX56.
PaxDbiQ9CX56.
PRIDEiQ9CX56.

PTM databases

iPTMnetiQ9CX56.
PhosphoSitePlusiQ9CX56.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030591.
ExpressionAtlasiQ9CX56. baseline and differential.
GenevisibleiQ9CX56. MM.

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits including PSMD8, a base containing 6 ATPases and few additional components.By similarity

Protein-protein interaction databases

BioGridi208250. 1 interactor.
IntActiQ9CX56. 2 interactors.
MINTiMINT-1869975.
STRINGi10090.ENSMUSP00000051657.

Structurei

3D structure databases

ProteinModelPortaliQ9CX56.
SMRiQ9CX56.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini165 – 334PCIPROSITE-ProRule annotationAdd BLAST170

Sequence similaritiesi

Belongs to the proteasome subunit S14 family.Curated

Phylogenomic databases

eggNOGiKOG3151. Eukaryota.
ENOG410XXP2. LUCA.
GeneTreeiENSGT00390000014682.
HOGENOMiHOG000196008.
HOVERGENiHBG024389.
InParanoidiQ9CX56.
KOiK03031.
OMAiGAFWSIR.
OrthoDBiEOG091G0G73.
PhylomeDBiQ9CX56.
TreeFamiTF106233.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiView protein in InterPro
IPR006746. 26S_Psome_Rpn12.
IPR033464. CSN8_PSD8_EIF3K.
IPR036388. WH-like_DNA-bd_sf.
PANTHERiPTHR12387. PTHR12387. 1 hit.
PfamiView protein in Pfam
PF10075. CSN8_PSD8_EIF3K. 1 hit.
PROSITEiView protein in PROSITE
PS50250. PCI. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CX56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFIKGRAAKT PRGEPRRSSR GGRKLAVVAP PPVLGSTSRP HFRRESIARR
60 70 80 90 100
RCRKSGRRLA ASRKMAATAA TVNGSTTVSS SGPAATSVGI LQAAAGMYEQ
110 120 130 140 150
LKDEWNRKNP NLSKCGEELG RLKLVLLELN FLPTTGTKLT KQQLILARDI
160 170 180 190 200
LEIGAQWSIL CKDIPSFERY MAQLKCYYFD YKEQLPESAY MHQLLGLNLL
210 220 230 240 250
FLLSQNRVAE FHTELERLPA KDIQTNVYIK HPVSLEQYLM EGSYNKVFLA
260 270 280 290 300
KGNIPAESYT FFIDILLDTI RDEIAGCIEK AYEKILFAEA TRILFFSTPK
310 320 330 340 350
KMTDYAKKRG WVLGPNNYYS FASQQQKPED STIPSTELAK QVIEYARQLE

MIV
Length:353
Mass (Da):39,930
Last modified:October 5, 2010 - v2
Checksum:i45C8836045CDDDF6
GO

Sequence cautioni

The sequence BAB32006 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC164564 Genomic DNA. No translation available.
AK020132 mRNA. Translation: BAB32006.2. Different initiation.
CCDSiCCDS52169.1.
RefSeqiNP_080821.3. NM_026545.3.
UniGeneiMm.273152.

Genome annotation databases

EnsembliENSMUST00000059642; ENSMUSP00000051657; ENSMUSG00000030591.
ENSMUST00000186182; ENSMUSP00000139514; ENSMUSG00000030591.
GeneIDi57296.
KEGGimmu:57296.
UCSCiuc009gbb.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiPSMD8_MOUSE
AccessioniPrimary (citable) accession number: Q9CX56
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: October 5, 2010
Last modified: November 22, 2017
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

It is uncertain whether Met-1 or Met-65 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families