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Protein

IST1 homolog

Gene

Ist1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Proposed to be involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells (By similarity).By similarity

GO - Molecular functioni

  1. MIT domain binding Source: MGI
  2. protein complex binding Source: MGI
  3. protein domain specific binding Source: MGI

GO - Biological processi

  1. abscission Source: MGI
  2. cell division Source: MGI
  3. cytokinesis Source: Ensembl
  4. establishment of protein localization Source: MGI
  5. positive regulation of collateral sprouting Source: MGI
  6. positive regulation of proteolysis Source: MGI
  7. protein localization Source: MGI
  8. viral capsid secondary envelopment Source: MGI
  9. viral release from host cell Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Names & Taxonomyi

Protein namesi
Recommended name:
IST1 homolog
Gene namesi
Name:Ist1
Synonyms:Kiaa0174
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:1919205. Ist1.

Subcellular locationi

Cytoplasmic vesicle By similarity
Note: Localizes to the midbody of dividing cells.By similarity

GO - Cellular componenti

  1. centrosome Source: MGI
  2. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  3. cytosol Source: MGI
  4. endoplasmic reticulum-Golgi intermediate compartment Source: MGI
  5. extracellular vesicular exosome Source: MGI
  6. Flemming body Source: MGI
  7. midbody Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362IST1 homologPRO_0000050728Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431PhosphotyrosineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9CX00.
PaxDbiQ9CX00.
PRIDEiQ9CX00.

PTM databases

PhosphoSiteiQ9CX00.

Expressioni

Gene expression databases

BgeeiQ9CX00.
CleanExiMM_2400003C14RIK.
GenevestigatoriQ9CX00.

Interactioni

Subunit structurei

Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with the ESCRT-I complex. Interacts with MITD1, in competition with VSP4 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9CX00. 2 interactions.
MINTiMINT-4125720.

Structurei

3D structure databases

ProteinModelPortaliQ9CX00.
SMRiQ9CX00. Positions 2-187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IST1 family.Curated

Phylogenomic databases

eggNOGiNOG273475.
GeneTreeiENSGT00390000007453.
HOGENOMiHOG000205346.
InParanoidiQ9CX00.
OMAiDTYDNFV.
OrthoDBiEOG7K3TNC.
PhylomeDBiQ9CX00.
TreeFamiTF314258.

Family and domain databases

InterProiIPR005061. DUF292_euk.
[Graphical view]
PfamiPF03398. Ist1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CX00-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLGSGFKAER LRVNLRLVIN RLKLLEKKKT ELAQKARKEI ADYLAAGKDE
60 70 80 90 100
RARIRVEHII REDYLVEAME ILELYCDLLL ARFGLIQSMK ELDSGLAESV
110 120 130 140 150
STLIWAAPRL QSEVAELKIV ADQLCAKYSK EYGKLCRTNQ IGTVNDRLMH
160 170 180 190 200
KLSVEAPPKI LVERYLIEIA KNYNVPYEPD SVVMAEAPVG VETDLIDVGF
210 220 230 240 250
TDDVKKGGPG RGGGGGFTAP VGGPDGIVPM PMPMPMPSPN APFAYPLPKG
260 270 280 290 300
PSDFSGLPVG TYQAFPNIHP PQIPATPPSY ESVDDINGDK TVSSAQIVGP
310 320 330 340 350
KPEAPAKPPS RPVDNYNTFV LPELPSVPDT LPTASAGAST SASEDIDFDD
360
LSRRFEELKK KT
Length:362
Mass (Da):39,468
Last modified:June 1, 2001 - v1
Checksum:iF3FB378DDBCD8BE7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti252 – 2565SDFSG → VSMCV in BAC65497. (PubMed:12693553)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010262 mRNA. Translation: BAB26804.1.
AK088192 mRNA. Translation: BAC40200.1.
AK152830 mRNA. Translation: BAE31530.1.
BC017605 mRNA. Translation: AAH17605.1.
AK122215 mRNA. Translation: BAC65497.1.
CCDSiCCDS22655.1.
RefSeqiNP_082294.1. NM_028018.2.
UniGeneiMm.290036.

Genome annotation databases

EnsembliENSMUST00000034164; ENSMUSP00000034164; ENSMUSG00000031729.
GeneIDi71955.
KEGGimmu:71955.
UCSCiuc009niy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010262 mRNA. Translation: BAB26804.1.
AK088192 mRNA. Translation: BAC40200.1.
AK152830 mRNA. Translation: BAE31530.1.
BC017605 mRNA. Translation: AAH17605.1.
AK122215 mRNA. Translation: BAC65497.1.
CCDSiCCDS22655.1.
RefSeqiNP_082294.1. NM_028018.2.
UniGeneiMm.290036.

3D structure databases

ProteinModelPortaliQ9CX00.
SMRiQ9CX00. Positions 2-187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CX00. 2 interactions.
MINTiMINT-4125720.

PTM databases

PhosphoSiteiQ9CX00.

Proteomic databases

MaxQBiQ9CX00.
PaxDbiQ9CX00.
PRIDEiQ9CX00.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034164; ENSMUSP00000034164; ENSMUSG00000031729.
GeneIDi71955.
KEGGimmu:71955.
UCSCiuc009niy.2. mouse.

Organism-specific databases

CTDi9798.
MGIiMGI:1919205. Ist1.
RougeiSearch...

Phylogenomic databases

eggNOGiNOG273475.
GeneTreeiENSGT00390000007453.
HOGENOMiHOG000205346.
InParanoidiQ9CX00.
OMAiDTYDNFV.
OrthoDBiEOG7K3TNC.
PhylomeDBiQ9CX00.
TreeFamiTF314258.

Miscellaneous databases

NextBioi335044.
PROiQ9CX00.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CX00.
CleanExiMM_2400003C14RIK.
GenevestigatoriQ9CX00.

Family and domain databases

InterProiIPR005061. DUF292_euk.
[Graphical view]
PfamiPF03398. Ist1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow and Thymus.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 35-256.
    Tissue: Brain.

Entry informationi

Entry nameiIST1_MOUSE
AccessioniPrimary (citable) accession number: Q9CX00
Secondary accession number(s): Q80U68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: June 1, 2001
Last modified: February 4, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.