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Protein

DNA helicase MCM8

Gene

Mcm8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G1 phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi447 – 454ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cellular response to DNA damage stimulus Source: UniProtKB
  • DNA replication initiation Source: InterPro
  • double-strand break repair via homologous recombination Source: UniProtKB
  • female gamete generation Source: UniProtKB
  • male gamete generation Source: UniProtKB

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA damage, DNA repair, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-176187 Activation of ATR in response to replication stress
R-MMU-68689 CDC6 association with the ORC:origin complex
R-MMU-68827 CDT1 association with the CDC6:ORC:origin complex
R-MMU-68867 Assembly of the pre-replicative complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-68962 Activation of the pre-replicative complex

Names & Taxonomyi

Protein namesi
Recommended name:
DNA helicase MCM8 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance 8
Gene namesi
Name:Mcm8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1913884 Mcm8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are viable but are sterile due to defects in double-strand break repair during gametogenesis. Testes are apoptotic and contain spermatocytes that have persistent DNA damage and unsynapsed chromosomes due to defective homologous recombinatio. Ovaries are characterized by an early block of follicle development, and they later develop tumors.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941261 – 833DNA helicase MCM8Add BLAST833

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei623PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9CWV1
PeptideAtlasiQ9CWV1
PRIDEiQ9CWV1

PTM databases

iPTMnetiQ9CWV1
PhosphoSitePlusiQ9CWV1

Expressioni

Gene expression databases

BgeeiENSMUSG00000027353
GenevisibleiQ9CWV1 MM

Interactioni

Subunit structurei

Interacts with CDC6 and ORC2 (By similarity). Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM8.By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066842

Structurei

3D structure databases

ProteinModelPortaliQ9CWV1
SMRiQ9CWV1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini395 – 602MCMAdd BLAST208

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0480 Eukaryota
COG1241 LUCA
GeneTreeiENSGT00910000144163
HOGENOMiHOG000224129
HOVERGENiHBG031700
InParanoidiQ9CWV1
KOiK10737
OMAiNVRANYY
OrthoDBiEOG091G01BH
PhylomeDBiQ9CWV1
TreeFamiTF323155

Family and domain databases

InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR031327 MCM
IPR001208 MCM_dom
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS50051 MCM_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CWV1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGAYRGRGF GRGRFQSWKR GRGGGNFSGR WRERENRVDL NEASGKHASA
60 70 80 90 100
QASQPLLQQS TLDQFIPYKG WKLYFSEVYS NNSPFIEKIQ AFEKFFTRHI
110 120 130 140 150
DLYDKDEIER KGSILVDFKE LTKADEITNL IPDIENALRD APEKTLACMG
160 170 180 190 200
LAIHQVLTKD LERHAAELQA QEGLSNGGET MVNVPHIYAR VYNYEPLTHL
210 220 230 240 250
KNIRATCYGK YISIRGTVVR VSNIKPLCTN MAFQCAACGE IQSFPLPDGK
260 270 280 290 300
YTLPTKCPVP ACRGRSFAPL RSSPLTVTLD WQLIKIQELM SDAQREAGRI
310 320 330 340 350
PRTIECELVH DLVDSCVPGD TVTVTGIVKV SNSEEGSRNK NDKCMFLLYI
360 370 380 390 400
EANSVSNSKG PKAQTAEDGC KHGTLMEFSL KDLYAIREIQ AEENLLKLVV
410 420 430 440 450
NSLCPVIFGH ELVKAGLTLA LFGGSQKYAD DKNRIPIRGD PHVLIVGDPG
460 470 480 490 500
LGKSQMLQAA CNVAPRGVYV CGNTTTSSGL TVTLSKDSSS GDFALEAGAL
510 520 530 540 550
VLGDQGICGI DEFDKMGNQH QALLEAMEQQ SISLAKAGVV CSLPARTSII
560 570 580 590 600
AAANPVGGHY NKARTVSENL KMGSALLSRF DLVFILLDTP NEQHDHLLSE
610 620 630 640 650
HVIAIRAGKQ KAVSSATVTR VLSQDSNTSV LEVVSEKPLS ERLKVAPGEQ
660 670 680 690 700
TDPIPHQLLR KYIGYARQYV HPRLSTDAAQ ALQDFYLELR KQSQRVGSSP
710 720 730 740 750
ITTRQLESLI RLTEARARLE LREEATREDA EDIIEIMKHS MLGTYSDEFG
760 770 780 790 800
NLDFERSQHG SGMSNRSTAK RFISALNSIA ERTYNNIFQY HQLRQIAKEL
810 820 830
NIQVADFENF IGSLNDQGYL LKKGPKIYQL QTM
Length:833
Mass (Da):92,371
Last modified:September 27, 2005 - v3
Checksum:iB45385BFB778B9E0
GO
Isoform 2 (identifier: Q9CWV1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-77: Missing.

Note: No experimental confirmation available.
Show »
Length:805
Mass (Da):89,101
Checksum:i1990CBFEEAA15484
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti379S → Y in BAE21892 (PubMed:16141072).Curated1
Sequence conflicti550I → V in AAH46780 (PubMed:15489334).Curated1
Sequence conflicti749F → L in BAB26885 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01578650 – 77Missing in isoform 2. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010365 mRNA Translation: BAB26885.1
AK133858 mRNA Translation: BAE21892.1
AL929562 Genomic DNA Translation: CAM26898.1
AL929562 Genomic DNA Translation: CAM26899.1
CH466519 Genomic DNA Translation: EDL28362.1
BC046780 mRNA Translation: AAH46780.1
BC052070 mRNA Translation: AAH52070.1
CCDSiCCDS16778.1 [Q9CWV1-2]
CCDS71152.1 [Q9CWV1-1]
RefSeqiNP_001277983.1, NM_001291054.1 [Q9CWV1-1]
NP_079952.2, NM_025676.4 [Q9CWV1-2]
UniGeneiMm.157070

Genome annotation databases

EnsembliENSMUST00000028831; ENSMUSP00000028831; ENSMUSG00000027353 [Q9CWV1-1]
ENSMUST00000066559; ENSMUSP00000066842; ENSMUSG00000027353 [Q9CWV1-2]
GeneIDi66634
KEGGimmu:66634
UCSCiuc008mni.2 mouse [Q9CWV1-2]
uc008mnj.2 mouse [Q9CWV1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMCM8_MOUSE
AccessioniPrimary (citable) accession number: Q9CWV1
Secondary accession number(s): A2AVM8
, A2AVM9, Q3UZG5, Q80US2, Q80VI0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: September 27, 2005
Last modified: May 23, 2018
This is version 132 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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