Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DNA helicase MCM8

Gene

Mcm8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8-MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G1 phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi447 – 4548ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cellular response to DNA damage stimulus Source: UniProtKB
  • DNA replication Source: UniProtKB-KW
  • double-strand break repair via homologous recombination Source: UniProtKB
  • female gamete generation Source: UniProtKB
  • male gamete generation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA damage, DNA repair, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-68689. CDC6 association with the ORC:origin complex.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68867. Assembly of the pre-replicative complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-68962. Activation of the pre-replicative complex.
R-MMU-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA helicase MCM8 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance 8
Gene namesi
Name:Mcm8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1913884. Mcm8.

Subcellular locationi

  • Nucleus By similarity

  • Note: Localizes to nuclear foci and colocalizes with RAD51.By similarity

GO - Cellular componenti

  • MCM8-MCM9 complex Source: UniProtKB
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are viable but are sterile due to defects in double-strand break repair during gametogenesis. Testes are apoptotic and contain spermatocytes that have persistent DNA damage and unsynapsed chromosomes due to defective homologous recombinatio. Ovaries are characterized by an early block of follicle development, and they later develop tumors.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 833833DNA helicase MCM8PRO_0000194126Add
BLAST

Proteomic databases

MaxQBiQ9CWV1.
PaxDbiQ9CWV1.
PRIDEiQ9CWV1.

PTM databases

PhosphoSiteiQ9CWV1.

Expressioni

Gene expression databases

BgeeiQ9CWV1.
GenevisibleiQ9CWV1. MM.

Interactioni

Subunit structurei

Interacts with CDC6 and ORC2 (By similarity). Component of the MCM8-MCM9 complex, which forms a hexamer composed of MCM8 and MCM8.By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066842.

Structurei

3D structure databases

ProteinModelPortaliQ9CWV1.
SMRiQ9CWV1. Positions 72-746.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini395 – 602208MCMAdd
BLAST

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiKOG0480. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00790000123057.
HOGENOMiHOG000224129.
HOVERGENiHBG031700.
InParanoidiQ9CWV1.
KOiK10737.
OMAiLIVNSLC.
OrthoDBiEOG7966G8.
PhylomeDBiQ9CWV1.
TreeFamiTF323155.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR001208. MCM_dom.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS50051. MCM_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CWV1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGAYRGRGF GRGRFQSWKR GRGGGNFSGR WRERENRVDL NEASGKHASA
60 70 80 90 100
QASQPLLQQS TLDQFIPYKG WKLYFSEVYS NNSPFIEKIQ AFEKFFTRHI
110 120 130 140 150
DLYDKDEIER KGSILVDFKE LTKADEITNL IPDIENALRD APEKTLACMG
160 170 180 190 200
LAIHQVLTKD LERHAAELQA QEGLSNGGET MVNVPHIYAR VYNYEPLTHL
210 220 230 240 250
KNIRATCYGK YISIRGTVVR VSNIKPLCTN MAFQCAACGE IQSFPLPDGK
260 270 280 290 300
YTLPTKCPVP ACRGRSFAPL RSSPLTVTLD WQLIKIQELM SDAQREAGRI
310 320 330 340 350
PRTIECELVH DLVDSCVPGD TVTVTGIVKV SNSEEGSRNK NDKCMFLLYI
360 370 380 390 400
EANSVSNSKG PKAQTAEDGC KHGTLMEFSL KDLYAIREIQ AEENLLKLVV
410 420 430 440 450
NSLCPVIFGH ELVKAGLTLA LFGGSQKYAD DKNRIPIRGD PHVLIVGDPG
460 470 480 490 500
LGKSQMLQAA CNVAPRGVYV CGNTTTSSGL TVTLSKDSSS GDFALEAGAL
510 520 530 540 550
VLGDQGICGI DEFDKMGNQH QALLEAMEQQ SISLAKAGVV CSLPARTSII
560 570 580 590 600
AAANPVGGHY NKARTVSENL KMGSALLSRF DLVFILLDTP NEQHDHLLSE
610 620 630 640 650
HVIAIRAGKQ KAVSSATVTR VLSQDSNTSV LEVVSEKPLS ERLKVAPGEQ
660 670 680 690 700
TDPIPHQLLR KYIGYARQYV HPRLSTDAAQ ALQDFYLELR KQSQRVGSSP
710 720 730 740 750
ITTRQLESLI RLTEARARLE LREEATREDA EDIIEIMKHS MLGTYSDEFG
760 770 780 790 800
NLDFERSQHG SGMSNRSTAK RFISALNSIA ERTYNNIFQY HQLRQIAKEL
810 820 830
NIQVADFENF IGSLNDQGYL LKKGPKIYQL QTM
Length:833
Mass (Da):92,371
Last modified:September 27, 2005 - v3
Checksum:iB45385BFB778B9E0
GO
Isoform 2 (identifier: Q9CWV1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     50-77: Missing.

Note: No experimental confirmation available.
Show »
Length:805
Mass (Da):89,101
Checksum:i1990CBFEEAA15484
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti379 – 3791S → Y in BAE21892 (PubMed:16141072).Curated
Sequence conflicti550 – 5501I → V in AAH46780 (PubMed:15489334).Curated
Sequence conflicti749 – 7491F → L in BAB26885 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei50 – 7728Missing in isoform 2. 1 PublicationVSP_015786Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010365 mRNA. Translation: BAB26885.1.
AK133858 mRNA. Translation: BAE21892.1.
AL929562 Genomic DNA. Translation: CAM26898.1.
AL929562 Genomic DNA. Translation: CAM26899.1.
CH466519 Genomic DNA. Translation: EDL28362.1.
BC046780 mRNA. Translation: AAH46780.1.
BC052070 mRNA. Translation: AAH52070.1.
CCDSiCCDS16778.1. [Q9CWV1-2]
CCDS71152.1. [Q9CWV1-1]
RefSeqiNP_001277983.1. NM_001291054.1. [Q9CWV1-1]
NP_079952.2. NM_025676.4. [Q9CWV1-2]
UniGeneiMm.157070.

Genome annotation databases

EnsembliENSMUST00000028831; ENSMUSP00000028831; ENSMUSG00000027353. [Q9CWV1-1]
ENSMUST00000066559; ENSMUSP00000066842; ENSMUSG00000027353. [Q9CWV1-2]
GeneIDi66634.
KEGGimmu:66634.
UCSCiuc008mni.2. mouse. [Q9CWV1-2]
uc008mnj.2. mouse. [Q9CWV1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010365 mRNA. Translation: BAB26885.1.
AK133858 mRNA. Translation: BAE21892.1.
AL929562 Genomic DNA. Translation: CAM26898.1.
AL929562 Genomic DNA. Translation: CAM26899.1.
CH466519 Genomic DNA. Translation: EDL28362.1.
BC046780 mRNA. Translation: AAH46780.1.
BC052070 mRNA. Translation: AAH52070.1.
CCDSiCCDS16778.1. [Q9CWV1-2]
CCDS71152.1. [Q9CWV1-1]
RefSeqiNP_001277983.1. NM_001291054.1. [Q9CWV1-1]
NP_079952.2. NM_025676.4. [Q9CWV1-2]
UniGeneiMm.157070.

3D structure databases

ProteinModelPortaliQ9CWV1.
SMRiQ9CWV1. Positions 72-746.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066842.

PTM databases

PhosphoSiteiQ9CWV1.

Proteomic databases

MaxQBiQ9CWV1.
PaxDbiQ9CWV1.
PRIDEiQ9CWV1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028831; ENSMUSP00000028831; ENSMUSG00000027353. [Q9CWV1-1]
ENSMUST00000066559; ENSMUSP00000066842; ENSMUSG00000027353. [Q9CWV1-2]
GeneIDi66634.
KEGGimmu:66634.
UCSCiuc008mni.2. mouse. [Q9CWV1-2]
uc008mnj.2. mouse. [Q9CWV1-1]

Organism-specific databases

CTDi84515.
MGIiMGI:1913884. Mcm8.

Phylogenomic databases

eggNOGiKOG0480. Eukaryota.
COG1241. LUCA.
GeneTreeiENSGT00790000123057.
HOGENOMiHOG000224129.
HOVERGENiHBG031700.
InParanoidiQ9CWV1.
KOiK10737.
OMAiLIVNSLC.
OrthoDBiEOG7966G8.
PhylomeDBiQ9CWV1.
TreeFamiTF323155.

Enzyme and pathway databases

ReactomeiR-MMU-176187. Activation of ATR in response to replication stress.
R-MMU-68689. CDC6 association with the ORC:origin complex.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68867. Assembly of the pre-replicative complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-68962. Activation of the pre-replicative complex.
R-MMU-69300. Removal of licensing factors from origins.

Miscellaneous databases

NextBioi322234.
PROiQ9CWV1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CWV1.
GenevisibleiQ9CWV1. MM.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR031327. MCM.
IPR001208. MCM_dom.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
SMARTiSM00382. AAA. 1 hit.
SM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 2 hits.
PROSITEiPS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Embryonic stem cell.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Fetal brain.
  5. "MCM8- and MCM9-deficient mice reveal gametogenesis defects and genome instability due to impaired homologous recombination."
    Lutzmann M., Grey C., Traver S., Ganier O., Maya-Mendoza A., Ranisavljevic N., Bernex F., Nishiyama A., Montel N., Gavois E., Forichon L., de Massy B., Mechali M.
    Mol. Cell 47:523-534(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE MCM8-MCM9 COMPLEX, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMCM8_MOUSE
AccessioniPrimary (citable) accession number: Q9CWV1
Secondary accession number(s): A2AVM8
, A2AVM9, Q3UZG5, Q80US2, Q80VI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: September 27, 2005
Last modified: January 20, 2016
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.