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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1

Gene

Ddah1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.1 Publication

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Enzyme regulationi

Inhibited by zinc ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301Substrate; via carbonyl oxygenBy similarity
Binding sitei73 – 731SubstrateBy similarity
Binding sitei78 – 781SubstrateBy similarity
Binding sitei79 – 791SubstrateBy similarity
Binding sitei98 – 981SubstrateBy similarity
Binding sitei145 – 1451SubstrateBy similarity
Active sitei173 – 1731Proton donorBy similarity
Binding sitei268 – 2681Substrate; via carbonyl oxygenBy similarity
Active sitei274 – 2741NucleophileBy similarity
Metal bindingi274 – 2741ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.3.18. 3474.
ReactomeiR-MMU-203615. eNOS activation.

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (EC:3.5.3.18)
Short name:
DDAH-1
Short name:
Dimethylarginine dimethylaminohydrolase 1
Alternative name(s):
DDAHI
Dimethylargininase-1
Gene namesi
Name:Ddah1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1916469. Ddah1.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Death at embryonic stages. Heterozygous mice show no visible phenotype, but have higher than normal tissue and plasma levels of asymmetric dimethylarginine (ADMA). They have increased mean arterial blood pressure and systemic vascular resistance, and decreased cardiac output and heart rate, probably due to reduced levels of nitric oxide (NO).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 285284N(G),N(G)-dimethylarginine dimethylaminohydrolase 1PRO_0000171119Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei33 – 331PhosphoserineBy similarity
Modified residuei222 – 2221S-nitrosocysteineBy similarity
Modified residuei274 – 2741S-nitrosocysteineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein, S-nitrosylation

Proteomic databases

MaxQBiQ9CWS0.
PaxDbiQ9CWS0.
PeptideAtlasiQ9CWS0.
PRIDEiQ9CWS0.

2D gel databases

REPRODUCTION-2DPAGEQ9CWS0.

PTM databases

iPTMnetiQ9CWS0.
PhosphoSiteiQ9CWS0.

Expressioni

Tissue specificityi

Detected in skeletal muscle, lung, heart and brain (at protein level). Detected in liver, kidney and lung.1 Publication

Gene expression databases

BgeeiENSMUSG00000028194.
CleanExiMM_DDAH1.
ExpressionAtlasiQ9CWS0. baseline and differential.
GenevisibleiQ9CWS0. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi213300. 1 interaction.
IntActiQ9CWS0. 4 interactions.
MINTiMINT-1854424.
STRINGi10090.ENSMUSP00000029845.

Structurei

3D structure databases

ProteinModelPortaliQ9CWS0.
SMRiQ9CWS0. Positions 8-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiENOG410IFS6. Eukaryota.
COG1834. LUCA.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiQ9CWS0.
KOiK01482.
OMAiCNGIALI.
OrthoDBiEOG091G0M5S.
PhylomeDBiQ9CWS0.
TreeFamiTF314737.

Family and domain databases

InterProiIPR033199. DDAH/AD.
IPR033197. DDAH1.
[Graphical view]
PANTHERiPTHR12737. PTHR12737. 1 hit.
PTHR12737:SF17. PTHR12737:SF17. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CWS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLGHPSAF GRATHAVVRA PPESLCRHAL RRSQGEEVDF ARAERQHELY
60 70 80 90 100
VGVLGSKLGL QVVQLPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK
110 120 130 140 150
EVDMMKEALE KLQLNIVEMK DENATLDGGD VLFTGREFFV GLSKRTNQRG
160 170 180 190 200
AEILADTFKD YAVSTVPVAD SLHLKSFCSM AGPNLIAIGS SESAQKALKI
210 220 230 240 250
MQQMSDHRYD KLTVPDDMAA NCIYLNIPSK GHVLLHRTPE EYPESAKVYE
260 270 280
KLKDHLLIPV SNSEMEKVDG LLTCCSVFIN KKIDS
Length:285
Mass (Da):31,381
Last modified:January 23, 2007 - v3
Checksum:i290B8DDF5AAE7928
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010430 mRNA. Translation: BAB26932.1.
AK143507 mRNA. Translation: BAE25405.1.
AK148907 mRNA. Translation: BAE28696.1.
BC034505 mRNA. Translation: AAH34505.1.
CCDSiCCDS17896.1.
RefSeqiNP_081269.1. NM_026993.3.
UniGeneiMm.234247.

Genome annotation databases

EnsembliENSMUST00000029845; ENSMUSP00000029845; ENSMUSG00000028194.
GeneIDi69219.
KEGGimmu:69219.
UCSCiuc008rqr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010430 mRNA. Translation: BAB26932.1.
AK143507 mRNA. Translation: BAE25405.1.
AK148907 mRNA. Translation: BAE28696.1.
BC034505 mRNA. Translation: AAH34505.1.
CCDSiCCDS17896.1.
RefSeqiNP_081269.1. NM_026993.3.
UniGeneiMm.234247.

3D structure databases

ProteinModelPortaliQ9CWS0.
SMRiQ9CWS0. Positions 8-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi213300. 1 interaction.
IntActiQ9CWS0. 4 interactions.
MINTiMINT-1854424.
STRINGi10090.ENSMUSP00000029845.

PTM databases

iPTMnetiQ9CWS0.
PhosphoSiteiQ9CWS0.

2D gel databases

REPRODUCTION-2DPAGEQ9CWS0.

Proteomic databases

MaxQBiQ9CWS0.
PaxDbiQ9CWS0.
PeptideAtlasiQ9CWS0.
PRIDEiQ9CWS0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029845; ENSMUSP00000029845; ENSMUSG00000028194.
GeneIDi69219.
KEGGimmu:69219.
UCSCiuc008rqr.2. mouse.

Organism-specific databases

CTDi23576.
MGIiMGI:1916469. Ddah1.

Phylogenomic databases

eggNOGiENOG410IFS6. Eukaryota.
COG1834. LUCA.
GeneTreeiENSGT00390000009331.
HOGENOMiHOG000161035.
HOVERGENiHBG055937.
InParanoidiQ9CWS0.
KOiK01482.
OMAiCNGIALI.
OrthoDBiEOG091G0M5S.
PhylomeDBiQ9CWS0.
TreeFamiTF314737.

Enzyme and pathway databases

BRENDAi3.5.3.18. 3474.
ReactomeiR-MMU-203615. eNOS activation.

Miscellaneous databases

ChiTaRSiDdah1. mouse.
PROiQ9CWS0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028194.
CleanExiMM_DDAH1.
ExpressionAtlasiQ9CWS0. baseline and differential.
GenevisibleiQ9CWS0. MM.

Family and domain databases

InterProiIPR033199. DDAH/AD.
IPR033197. DDAH1.
[Graphical view]
PANTHERiPTHR12737. PTHR12737. 1 hit.
PTHR12737:SF17. PTHR12737:SF17. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDDAH1_MOUSE
AccessioniPrimary (citable) accession number: Q9CWS0
Secondary accession number(s): Q3UF65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.