Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9CWR8

- DNM3L_MOUSE

UniProt

Q9CWR8 - DNM3L_MOUSE

Protein

DNA (cytosine-5)-methyltransferase 3-like

Gene

Dnmt3l

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri86 – 11631GATA-type; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri127 – 18357PHD-type; atypicalPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. enzyme activator activity Source: MGI
    2. enzyme binding Source: UniProtKB
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct

    GO - Biological processi

    1. chorionic trophoblast cell differentiation Source: MGI
    2. genetic imprinting Source: MGI
    3. in utero embryonic development Source: MGI
    4. methylation Source: MGI
    5. negative regulation of transcription, DNA-templated Source: UniProtKB
    6. placenta development Source: MGI
    7. positive regulation of catalytic activity Source: GOC

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    REBASEi6168. M.MmuDnmt3L.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA (cytosine-5)-methyltransferase 3-like
    Gene namesi
    Name:Dnmt3l
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:1859287. Dnmt3l.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. condensed nuclear chromosome Source: MGI
    2. cytoplasm Source: MGI
    3. cytosol Source: Ensembl
    4. nuclear heterochromatin Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi297 – 2971F → A: Loss of binding to DNMT3A. 1 Publication
    Mutagenesisi297 – 2971F → E: Loss of binding to DNMT3A. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 421421DNA (cytosine-5)-methyltransferase 3-likePRO_0000088048Add
    BLAST

    Proteomic databases

    PaxDbiQ9CWR8.
    PRIDEiQ9CWR8.

    PTM databases

    PhosphoSiteiQ9CWR8.

    Expressioni

    Tissue specificityi

    Expressed in testis, thymus, ovary, and heart.

    Gene expression databases

    ArrayExpressiQ9CWR8.
    BgeeiQ9CWR8.
    CleanExiMM_DNMT3L.
    GenevestigatoriQ9CWR8.

    Interactioni

    Subunit structurei

    Homodimer. Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with histone H3 (via N-terminus) By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Dnmt3aO885086EBI-3043871,EBI-995154
    Dnmt3bO885095EBI-3043871,EBI-7987547
    EZH2Q159102EBI-3043871,EBI-530054From a different organism.
    Ezh2Q6118810EBI-3043871,EBI-904311

    Protein-protein interaction databases

    BioGridi207659. 6 interactions.
    DIPiDIP-49029N.
    IntActiQ9CWR8. 6 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CWR8.
    SMRiQ9CWR8. Positions 68-415.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini75 – 207133ADDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ADD domain.PROSITE-ProRule annotation
    Contains 1 GATA-type zinc finger.PROSITE-ProRule annotation
    Contains 1 PHD-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri86 – 11631GATA-type; atypicalPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri127 – 18357PHD-type; atypicalPROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG69419.
    GeneTreeiENSGT00390000008341.
    HOGENOMiHOG000246422.
    HOVERGENiHBG051382.
    InParanoidiQ9CWR8.
    KOiK17400.
    OMAiTVPVWKR.
    OrthoDBiEOG7MWGW6.
    PhylomeDBiQ9CWR8.
    TreeFamiTF329039.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR025766. ADD.
    IPR011011. Znf_FYVE_PHD.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    SUPFAMiSSF57903. SSF57903. 1 hit.
    PROSITEiPS51533. ADD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9CWR8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGSRETPSSC SKTLETLDLE TSDSSSPDAD SPLEEQWLKS SPALKEDSVD    50
    VVLEDCKEPL SPSSPPTGRE MIRYEVKVNR RSIEDICLCC GTLQVYTRHP 100
    LFEGGLCAPC KDKFLESLFL YDDDGHQSYC TICCSGGTLF ICESPDCTRC 150
    YCFECVDILV GPGTSERINA MACWVCFLCL PFSRSGLLQR RKRWRHQLKA 200
    FHDQEGAGPM EIYKTVSAWK RQPVRVLSLF RNIDKVLKSL GFLESGSGSG 250
    GGTLKYVEDV TNVVRRDVEK WGPFDLVYGS TQPLGSSCDR CPGWYMFQFH 300
    RILQYALPRQ ESQRPFFWIF MDNLLLTEDD QETTTRFLQT EAVTLQDVRG 350
    RDYQNAMRVW SNIPGLKSKH APLTPKEEEY LQAQVRSRSK LDAPKVDLLV 400
    KNCLLPLREY FKYFSQNSLP L 421
    Length:421
    Mass (Da):47,993
    Last modified:June 1, 2001 - v1
    Checksum:i6C996D220C6F6D83
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ404467 mRNA. Translation: CAB94726.1.
    AF220524 mRNA. Translation: AAF73868.1.
    AK010434 mRNA. Translation: BAB26936.1.
    BC083147 mRNA. Translation: AAH83147.1.
    CCDSiCCDS23962.1.
    RefSeqiNP_001075164.1. NM_001081695.2.
    NP_001271126.1. NM_001284197.1.
    NP_062321.1. NM_019448.4.
    UniGeneiMm.13433.

    Genome annotation databases

    EnsembliENSMUST00000000746; ENSMUSP00000000746; ENSMUSG00000000730.
    ENSMUST00000138785; ENSMUSP00000121562; ENSMUSG00000000730.
    ENSMUST00000151242; ENSMUSP00000116970; ENSMUSG00000000730.
    GeneIDi54427.
    KEGGimmu:54427.
    UCSCiuc007fxe.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ404467 mRNA. Translation: CAB94726.1 .
    AF220524 mRNA. Translation: AAF73868.1 .
    AK010434 mRNA. Translation: BAB26936.1 .
    BC083147 mRNA. Translation: AAH83147.1 .
    CCDSi CCDS23962.1.
    RefSeqi NP_001075164.1. NM_001081695.2.
    NP_001271126.1. NM_001284197.1.
    NP_062321.1. NM_019448.4.
    UniGenei Mm.13433.

    3D structure databases

    ProteinModelPortali Q9CWR8.
    SMRi Q9CWR8. Positions 68-415.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 207659. 6 interactions.
    DIPi DIP-49029N.
    IntActi Q9CWR8. 6 interactions.

    Protein family/group databases

    REBASEi 6168. M.MmuDnmt3L.

    PTM databases

    PhosphoSitei Q9CWR8.

    Proteomic databases

    PaxDbi Q9CWR8.
    PRIDEi Q9CWR8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000746 ; ENSMUSP00000000746 ; ENSMUSG00000000730 .
    ENSMUST00000138785 ; ENSMUSP00000121562 ; ENSMUSG00000000730 .
    ENSMUST00000151242 ; ENSMUSP00000116970 ; ENSMUSG00000000730 .
    GeneIDi 54427.
    KEGGi mmu:54427.
    UCSCi uc007fxe.1. mouse.

    Organism-specific databases

    CTDi 29947.
    MGIi MGI:1859287. Dnmt3l.

    Phylogenomic databases

    eggNOGi NOG69419.
    GeneTreei ENSGT00390000008341.
    HOGENOMi HOG000246422.
    HOVERGENi HBG051382.
    InParanoidi Q9CWR8.
    KOi K17400.
    OMAi TVPVWKR.
    OrthoDBi EOG7MWGW6.
    PhylomeDBi Q9CWR8.
    TreeFami TF329039.

    Miscellaneous databases

    NextBioi 311304.
    PROi Q9CWR8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9CWR8.
    Bgeei Q9CWR8.
    CleanExi MM_DNMT3L.
    Genevestigatori Q9CWR8.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR025766. ADD.
    IPR011011. Znf_FYVE_PHD.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    SUPFAMi SSF57903. SSF57903. 1 hit.
    PROSITEi PS51533. ADD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and initial characterization of the mouse Dnmt3l gene."
      Aapola U., Lyle R., Krohn K., Antonarakis S.E., Peterson P.
      Cytogenet. Cell Genet. 92:122-126(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Full-length cDNA of a murine Dnmt3-like gene."
      Shaoping X., Hata K., Li E.
      Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryonic stem cell.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    5. "Dnmt3L and the establishment of maternal genomic imprints."
      Bourc'his D., Xu G.L., Lin C.S., Bollman B., Bestor T.H.
      Science 294:2536-2539(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L."
      Gowher H., Liebert K., Hermann A., Xu G., Jeltsch A.
      J. Biol. Chem. 280:13341-13348(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Formation of nucleoprotein filaments by mammalian DNA methyltransferase Dnmt3a in complex with regulator Dnmt3L."
      Jurkowska R.Z., Anspach N., Urbanke C., Jia D., Reinhardt R., Nellen W., Cheng X., Jeltsch A.
      Nucleic Acids Res. 36:6656-6663(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF PHE-297.

    Entry informationi

    Entry nameiDNM3L_MOUSE
    AccessioniPrimary (citable) accession number: Q9CWR8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3