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Q9CWR8 (DNM3L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA (cytosine-5)-methyltransferase 3-like
Gene names
Name:Dnmt3l
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Ref.5 Ref.6

Subunit structure

Homodimer. Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with histone H3 (via N-terminus) By similarity.

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in testis, thymus, ovary, and heart.

Sequence similarities

Contains 1 ADD domain.

Contains 1 GATA-type zinc finger.

Contains 1 PHD-type zinc finger.

Ontologies

Keywords
   Cellular componentNucleus
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processchorionic trophoblast cell differentiation

Inferred from mutant phenotype PubMed 16920095. Source: MGI

genetic imprinting

Inferred from mutant phenotype PubMed 16920095. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 11934864. Source: MGI

methylation

Inferred from mutant phenotype PubMed 16920095. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from sequence or structural similarity PubMed 12202768. Source: UniProtKB

placenta development

Inferred from mutant phenotype PubMed 16920095. Source: MGI

positive regulation of catalytic activity

Inferred from direct assay PubMed 16543361. Source: GOC

   Cellular_componentcondensed nuclear chromosome

Inferred from direct assay PubMed 16543361. Source: MGI

cytoplasm

Inferred from direct assay PubMed 11934864PubMed 16543361. Source: MGI

cytosol

Inferred from electronic annotation. Source: Ensembl

nuclear heterochromatin

Inferred from direct assay PubMed 11934864. Source: MGI

   Molecular_functionenzyme activator activity

Inferred from direct assay PubMed 16543361. Source: MGI

enzyme binding

Inferred from sequence or structural similarity PubMed 12202768. Source: UniProtKB

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 24074865. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dnmt3aO885086EBI-3043871,EBI-995154
Dnmt3bO885095EBI-3043871,EBI-7987547
EZH2Q159102EBI-3043871,EBI-530054From a different organism.
Ezh2Q6118810EBI-3043871,EBI-904311

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 421421DNA (cytosine-5)-methyltransferase 3-like
PRO_0000088048

Regions

Domain75 – 207133ADD
Zinc finger86 – 11631GATA-type; atypical
Zinc finger127 – 18357PHD-type; atypical

Experimental info

Mutagenesis2971F → A: Loss of binding to DNMT3A. Ref.7
Mutagenesis2971F → E: Loss of binding to DNMT3A. Ref.7

Sequences

Sequence LengthMass (Da)Tools
Q9CWR8 [UniParc].

Last modified June 1, 2001. Version 1.
Checksum: 6C996D220C6F6D83

FASTA42147,993
        10         20         30         40         50         60 
MGSRETPSSC SKTLETLDLE TSDSSSPDAD SPLEEQWLKS SPALKEDSVD VVLEDCKEPL 

        70         80         90        100        110        120 
SPSSPPTGRE MIRYEVKVNR RSIEDICLCC GTLQVYTRHP LFEGGLCAPC KDKFLESLFL 

       130        140        150        160        170        180 
YDDDGHQSYC TICCSGGTLF ICESPDCTRC YCFECVDILV GPGTSERINA MACWVCFLCL 

       190        200        210        220        230        240 
PFSRSGLLQR RKRWRHQLKA FHDQEGAGPM EIYKTVSAWK RQPVRVLSLF RNIDKVLKSL 

       250        260        270        280        290        300 
GFLESGSGSG GGTLKYVEDV TNVVRRDVEK WGPFDLVYGS TQPLGSSCDR CPGWYMFQFH 

       310        320        330        340        350        360 
RILQYALPRQ ESQRPFFWIF MDNLLLTEDD QETTTRFLQT EAVTLQDVRG RDYQNAMRVW 

       370        380        390        400        410        420 
SNIPGLKSKH APLTPKEEEY LQAQVRSRSK LDAPKVDLLV KNCLLPLREY FKYFSQNSLP 


L 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and initial characterization of the mouse Dnmt3l gene."
Aapola U., Lyle R., Krohn K., Antonarakis S.E., Peterson P.
Cytogenet. Cell Genet. 92:122-126(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Full-length cDNA of a murine Dnmt3-like gene."
Shaoping X., Hata K., Li E.
Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryonic stem cell.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo.
[5]"Dnmt3L and the establishment of maternal genomic imprints."
Bourc'his D., Xu G.L., Lin C.S., Bollman B., Bestor T.H.
Science 294:2536-2539(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L."
Gowher H., Liebert K., Hermann A., Xu G., Jeltsch A.
J. Biol. Chem. 280:13341-13348(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"Formation of nucleoprotein filaments by mammalian DNA methyltransferase Dnmt3a in complex with regulator Dnmt3L."
Jurkowska R.Z., Anspach N., Urbanke C., Jia D., Reinhardt R., Nellen W., Cheng X., Jeltsch A.
Nucleic Acids Res. 36:6656-6663(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF PHE-297.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ404467 mRNA. Translation: CAB94726.1.
AF220524 mRNA. Translation: AAF73868.1.
AK010434 mRNA. Translation: BAB26936.1.
BC083147 mRNA. Translation: AAH83147.1.
CCDSCCDS23962.1.
RefSeqNP_001075164.1. NM_001081695.2.
NP_001271126.1. NM_001284197.1.
NP_062321.1. NM_019448.4.
UniGeneMm.13433.

3D structure databases

ProteinModelPortalQ9CWR8.
SMRQ9CWR8. Positions 68-415.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid207659. 6 interactions.
DIPDIP-49029N.
IntActQ9CWR8. 6 interactions.

Protein family/group databases

REBASE6168. M.MmuDnmt3L.

PTM databases

PhosphoSiteQ9CWR8.

Proteomic databases

PaxDbQ9CWR8.
PRIDEQ9CWR8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000746; ENSMUSP00000000746; ENSMUSG00000000730.
ENSMUST00000138785; ENSMUSP00000121562; ENSMUSG00000000730.
ENSMUST00000151242; ENSMUSP00000116970; ENSMUSG00000000730.
GeneID54427.
KEGGmmu:54427.
UCSCuc007fxe.1. mouse.

Organism-specific databases

CTD29947.
MGIMGI:1859287. Dnmt3l.

Phylogenomic databases

eggNOGNOG69419.
GeneTreeENSGT00390000008341.
HOGENOMHOG000246422.
HOVERGENHBG051382.
InParanoidQ9CWR8.
KOK17400.
OMATVPVWKR.
OrthoDBEOG7MWGW6.
PhylomeDBQ9CWR8.
TreeFamTF329039.

Gene expression databases

ArrayExpressQ9CWR8.
BgeeQ9CWR8.
CleanExMM_DNMT3L.
GenevestigatorQ9CWR8.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR025766. ADD.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
SUPFAMSSF57903. SSF57903. 1 hit.
PROSITEPS51533. ADD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio311304.
PROQ9CWR8.
SOURCESearch...

Entry information

Entry nameDNM3L_MOUSE
AccessionPrimary (citable) accession number: Q9CWR8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 2001
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot