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Protein

DNA (cytosine-5)-methyltransferase 3-like

Gene

Dnmt3l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri86 – 11631GATA-type; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri127 – 18357PHD-type; atypicalPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. enzyme activator activity Source: MGI
  2. enzyme binding Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. chorionic trophoblast cell differentiation Source: MGI
  2. genetic imprinting Source: MGI
  3. in utero embryonic development Source: MGI
  4. methylation Source: MGI
  5. negative regulation of transcription, DNA-templated Source: UniProtKB
  6. placenta development Source: MGI
  7. positive regulation of catalytic activity Source: MGI
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_351456. DNA methylation.

Protein family/group databases

REBASEi6168. M.MmuDnmt3L.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase 3-like
Gene namesi
Name:Dnmt3l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1859287. Dnmt3l.

Subcellular locationi

  1. Nucleus Curated

GO - Cellular componenti

  1. condensed nuclear chromosome Source: MGI
  2. cytoplasm Source: MGI
  3. cytosol Source: MGI
  4. nuclear heterochromatin Source: MGI
  5. nucleoplasm Source: Reactome
  6. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi297 – 2971F → A: Loss of binding to DNMT3A. 1 Publication
Mutagenesisi297 – 2971F → E: Loss of binding to DNMT3A. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421DNA (cytosine-5)-methyltransferase 3-likePRO_0000088048Add
BLAST

Proteomic databases

PaxDbiQ9CWR8.
PRIDEiQ9CWR8.

PTM databases

PhosphoSiteiQ9CWR8.

Expressioni

Tissue specificityi

Expressed in testis, thymus, ovary, and heart.

Gene expression databases

BgeeiQ9CWR8.
CleanExiMM_DNMT3L.
ExpressionAtlasiQ9CWR8. baseline and differential.
GenevestigatoriQ9CWR8.

Interactioni

Subunit structurei

Homodimer. Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with histone H3 (via N-terminus) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Dnmt3aO885086EBI-3043871,EBI-995154
Dnmt3bO885095EBI-3043871,EBI-7987547
EZH2Q159102EBI-3043871,EBI-530054From a different organism.
Ezh2Q6118810EBI-3043871,EBI-904311

Protein-protein interaction databases

BioGridi207659. 6 interactions.
DIPiDIP-49029N.
IntActiQ9CWR8. 6 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9CWR8.
SMRiQ9CWR8. Positions 68-415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini75 – 207133ADDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ADD domain.PROSITE-ProRule annotation
Contains 1 GATA-type zinc finger.PROSITE-ProRule annotation
Contains 1 PHD-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri86 – 11631GATA-type; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri127 – 18357PHD-type; atypicalPROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG69419.
GeneTreeiENSGT00390000008341.
HOGENOMiHOG000246422.
HOVERGENiHBG051382.
InParanoidiQ9CWR8.
KOiK17400.
OMAiTVPVWKR.
OrthoDBiEOG7MWGW6.
PhylomeDBiQ9CWR8.
TreeFamiTF329039.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR025766. ADD.
IPR030486. DNMT3L.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23068:SF5. PTHR23068:SF5. 1 hit.
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS51533. ADD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CWR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSRETPSSC SKTLETLDLE TSDSSSPDAD SPLEEQWLKS SPALKEDSVD
60 70 80 90 100
VVLEDCKEPL SPSSPPTGRE MIRYEVKVNR RSIEDICLCC GTLQVYTRHP
110 120 130 140 150
LFEGGLCAPC KDKFLESLFL YDDDGHQSYC TICCSGGTLF ICESPDCTRC
160 170 180 190 200
YCFECVDILV GPGTSERINA MACWVCFLCL PFSRSGLLQR RKRWRHQLKA
210 220 230 240 250
FHDQEGAGPM EIYKTVSAWK RQPVRVLSLF RNIDKVLKSL GFLESGSGSG
260 270 280 290 300
GGTLKYVEDV TNVVRRDVEK WGPFDLVYGS TQPLGSSCDR CPGWYMFQFH
310 320 330 340 350
RILQYALPRQ ESQRPFFWIF MDNLLLTEDD QETTTRFLQT EAVTLQDVRG
360 370 380 390 400
RDYQNAMRVW SNIPGLKSKH APLTPKEEEY LQAQVRSRSK LDAPKVDLLV
410 420
KNCLLPLREY FKYFSQNSLP L
Length:421
Mass (Da):47,993
Last modified:June 1, 2001 - v1
Checksum:i6C996D220C6F6D83
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ404467 mRNA. Translation: CAB94726.1.
AF220524 mRNA. Translation: AAF73868.1.
AK010434 mRNA. Translation: BAB26936.1.
BC083147 mRNA. Translation: AAH83147.1.
CCDSiCCDS23962.1.
RefSeqiNP_001075164.1. NM_001081695.2.
NP_001271126.1. NM_001284197.1.
NP_062321.1. NM_019448.4.
UniGeneiMm.13433.

Genome annotation databases

EnsembliENSMUST00000000746; ENSMUSP00000000746; ENSMUSG00000000730.
ENSMUST00000138785; ENSMUSP00000121562; ENSMUSG00000000730.
ENSMUST00000151242; ENSMUSP00000116970; ENSMUSG00000000730.
GeneIDi54427.
KEGGimmu:54427.
UCSCiuc007fxe.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ404467 mRNA. Translation: CAB94726.1.
AF220524 mRNA. Translation: AAF73868.1.
AK010434 mRNA. Translation: BAB26936.1.
BC083147 mRNA. Translation: AAH83147.1.
CCDSiCCDS23962.1.
RefSeqiNP_001075164.1. NM_001081695.2.
NP_001271126.1. NM_001284197.1.
NP_062321.1. NM_019448.4.
UniGeneiMm.13433.

3D structure databases

ProteinModelPortaliQ9CWR8.
SMRiQ9CWR8. Positions 68-415.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207659. 6 interactions.
DIPiDIP-49029N.
IntActiQ9CWR8. 6 interactions.

Protein family/group databases

REBASEi6168. M.MmuDnmt3L.

PTM databases

PhosphoSiteiQ9CWR8.

Proteomic databases

PaxDbiQ9CWR8.
PRIDEiQ9CWR8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000746; ENSMUSP00000000746; ENSMUSG00000000730.
ENSMUST00000138785; ENSMUSP00000121562; ENSMUSG00000000730.
ENSMUST00000151242; ENSMUSP00000116970; ENSMUSG00000000730.
GeneIDi54427.
KEGGimmu:54427.
UCSCiuc007fxe.1. mouse.

Organism-specific databases

CTDi29947.
MGIiMGI:1859287. Dnmt3l.

Phylogenomic databases

eggNOGiNOG69419.
GeneTreeiENSGT00390000008341.
HOGENOMiHOG000246422.
HOVERGENiHBG051382.
InParanoidiQ9CWR8.
KOiK17400.
OMAiTVPVWKR.
OrthoDBiEOG7MWGW6.
PhylomeDBiQ9CWR8.
TreeFamiTF329039.

Enzyme and pathway databases

ReactomeiREACT_351456. DNA methylation.

Miscellaneous databases

NextBioi311304.
PROiQ9CWR8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CWR8.
CleanExiMM_DNMT3L.
ExpressionAtlasiQ9CWR8. baseline and differential.
GenevestigatoriQ9CWR8.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR025766. ADD.
IPR030486. DNMT3L.
IPR011011. Znf_FYVE_PHD.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR23068:SF5. PTHR23068:SF5. 1 hit.
SUPFAMiSSF57903. SSF57903. 1 hit.
PROSITEiPS51533. ADD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and initial characterization of the mouse Dnmt3l gene."
    Aapola U., Lyle R., Krohn K., Antonarakis S.E., Peterson P.
    Cytogenet. Cell Genet. 92:122-126(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Full-length cDNA of a murine Dnmt3-like gene."
    Shaoping X., Hata K., Li E.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic stem cell.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  5. "Dnmt3L and the establishment of maternal genomic imprints."
    Bourc'his D., Xu G.L., Lin C.S., Bollman B., Bestor T.H.
    Science 294:2536-2539(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Mechanism of stimulation of catalytic activity of Dnmt3A and Dnmt3B DNA-(cytosine-C5)-methyltransferases by Dnmt3L."
    Gowher H., Liebert K., Hermann A., Xu G., Jeltsch A.
    J. Biol. Chem. 280:13341-13348(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Formation of nucleoprotein filaments by mammalian DNA methyltransferase Dnmt3a in complex with regulator Dnmt3L."
    Jurkowska R.Z., Anspach N., Urbanke C., Jia D., Reinhardt R., Nellen W., Cheng X., Jeltsch A.
    Nucleic Acids Res. 36:6656-6663(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF PHE-297.

Entry informationi

Entry nameiDNM3L_MOUSE
AccessioniPrimary (citable) accession number: Q9CWR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 1, 2001
Last modified: April 29, 2015
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.