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Protein

Histone-lysine N-methyltransferase SMYD3

Gene

Smyd3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation. Also methylates 'Lys-5' of histone H4. Plays an important role in transcriptional activation as a member of an RNA polymerase complex. Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences.By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone].PROSITE-ProRule annotation

Enzyme regulationi

Histone methyltransferase activity strongly stimulated by HSPCA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei124S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei132S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei239S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei259S-adenosyl-L-methioninePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri49 – 87MYND-typePROSITE-ProRule annotationAdd BLAST39

GO - Molecular functioni

  • histone-lysine N-methyltransferase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II core binding Source: MGI
  • RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI

GO - Biological processi

  • cellular response to dexamethasone stimulus Source: MGI
  • establishment of protein localization Source: MGI
  • myotube cell development Source: MGI
  • negative regulation of protein kinase activity Source: MGI
  • nucleosome assembly Source: MGI
  • positive regulation of peptidyl-serine phosphorylation Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI

Keywordsi

Molecular functionChromatin regulator, Methyltransferase, Transferase
LigandMetal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

BRENDAi2.1.1.43 3474
ReactomeiR-MMU-3214841 PKMTs methylate histone lysines

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase SMYD3 (EC:2.1.1.43)
Alternative name(s):
SET and MYND domain-containing protein 3
Zinc finger MYND domain-containing protein 1
Gene namesi
Name:Smyd3
Synonyms:Zmynd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1916976 Smyd3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002183131 – 428Histone-lysine N-methyltransferase SMYD3Add BLAST428

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9CWR2
MaxQBiQ9CWR2
PaxDbiQ9CWR2
PRIDEiQ9CWR2

PTM databases

iPTMnetiQ9CWR2
PhosphoSitePlusiQ9CWR2

Expressioni

Gene expression databases

BgeeiENSMUSG00000055067
CleanExiMM_SMYD3
ExpressionAtlasiQ9CWR2 baseline and differential
GenevisibleiQ9CWR2 MM

Interactioni

Subunit structurei

Interacts with HSPCA. Interacts with HELZ. Interacts with POLR2A; the interaction may be indirect and may be mediated by HELZ. Interacts with HSP90AA1; this interaction enhances SMYD3 histone-lysine N-methyltransferase.By similarity

GO - Molecular functioni

  • RNA polymerase II core binding Source: MGI

Protein-protein interaction databases

BioGridi213640, 1 interactor
STRINGi10090.ENSMUSP00000117410

Structurei

3D structure databases

ProteinModelPortaliQ9CWR2
SMRiQ9CWR2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 240SETPROSITE-ProRule annotationAdd BLAST237

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 16S-adenosyl-L-methionine bindingBy similarity3
Regioni205 – 206S-adenosyl-L-methionine bindingBy similarity2
Regioni272 – 428C-terminal domain; essential for histone methyltransferase activity, nuclear localization and mediates interaction with HSP90AA1By similarityAdd BLAST157

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri49 – 87MYND-typePROSITE-ProRule annotationAdd BLAST39

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2084 Eukaryota
COG2940 LUCA
GeneTreeiENSGT00530000063077
HOGENOMiHOG000007850
HOVERGENiHBG105004
InParanoidiQ9CWR2
KOiK11426
OMAiDVCKVCL
OrthoDBiEOG091G066T
PhylomeDBiQ9CWR2
TreeFamiTF106487

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR025805 Hist-Lys_N-MeTrfase_Smyd3
IPR001214 SET_dom
IPR011990 TPR-like_helical_dom_sf
IPR002893 Znf_MYND
PfamiView protein in Pfam
PF00856 SET, 1 hit
PF01753 zf-MYND, 1 hit
SMARTiView protein in SMART
SM00317 SET, 1 hit
PROSITEiView protein in PROSITE
PS51574 SAM_MT43_2, 1 hit
PS50280 SET, 1 hit
PS01360 ZF_MYND_1, 1 hit
PS50865 ZF_MYND_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CWR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEALKVEKFT TANRGNGLRA VAPLRPGELL FRSDPLAYTV CKGSRGVVCD
60 70 80 90 100
RCLLGKEKLM RCSQCRIAKY CSAKCQKKAW PDHRRECSCL KSCKPRYPPD
110 120 130 140 150
SVRLLGRVIV KLMDEKPSES EKLYSFYDLE SNISKLTEDK KEGLRQLAMT
160 170 180 190 200
FQHFMREEIQ DASQLPPSFD LFEAFAKVIC NSFTICNAEM QEVGVGLYPS
210 220 230 240 250
MSLLNHSCDP NCSIVFNGPH LLLRAVREIE AGEELTICYL DMLMTSEERR
260 270 280 290 300
KQLRDQYCFE CDCIRCQTQD KDADMLTGDE QIWKEVQESL KKIEELKAHW
310 320 330 340 350
KWEQVLALCQ AIINSNSNRL PDINIYQLKV LDCAMDACIN LGMLEEALFY
360 370 380 390 400
AMRTMEPYRI FFPGSHPVRG VQVMKVGKLQ LHQGMFPQAM KNLRLAFDIM
410 420
KVTHGREHSL IEDLILLLEE CDANIRAS
Length:428
Mass (Da):49,126
Last modified:June 1, 2001 - v1
Checksum:i1EC765044E1FB0DA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti318N → D in AAH61485 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010447 mRNA Translation: BAB26947.1
AK044168 mRNA Translation: BAC31804.1
AK046829 mRNA Translation: BAC32887.1
BC052431 mRNA Translation: AAH52431.1
BC061485 mRNA Translation: AAH61485.1
CCDSiCCDS15560.1
RefSeqiNP_081464.1, NM_027188.3
XP_006497045.1, XM_006496982.3
XP_017167700.1, XM_017312211.1
XP_017167702.1, XM_017312213.1
UniGeneiMm.222338

Genome annotation databases

EnsembliENSMUST00000128302; ENSMUSP00000117410; ENSMUSG00000055067
GeneIDi69726
KEGGimmu:69726
UCSCiuc007dvj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSMYD3_MOUSE
AccessioniPrimary (citable) accession number: Q9CWR2
Secondary accession number(s): Q6P7V6, Q8BG90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health