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Protein

Diphthine methyl ester synthase

Gene

Dph5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.By similarity1 Publication

Catalytic activityi

4 S-adenosyl-L-methionine + 2-((3S)-3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2].By similarity

Pathwayi: peptidyl-diphthamide biosynthesis

This protein is involved in the pathway peptidyl-diphthamide biosynthesis, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway peptidyl-diphthamide biosynthesis and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei84S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei87S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei163S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei225S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei250S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-MMU-5358493. Synthesis of diphthamide-EEF2.
UniPathwayiUPA00559.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthine methyl ester synthase (EC:2.1.1.314)
Alternative name(s):
Diphthamide biosynthesis methyltransferase
Gene namesi
Name:Dph5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1916990. Dph5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001561341 – 281Diphthine methyl ester synthaseAdd BLAST281

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei171PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9CWQ0.
MaxQBiQ9CWQ0.
PaxDbiQ9CWQ0.
PeptideAtlasiQ9CWQ0.
PRIDEiQ9CWQ0.

PTM databases

PhosphoSitePlusiQ9CWQ0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033554.
CleanExiMM_DPH5.
ExpressionAtlasiQ9CWQ0. baseline and differential.
GenevisibleiQ9CWQ0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043730.

Structurei

3D structure databases

ProteinModelPortaliQ9CWQ0.
SMRiQ9CWQ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni112 – 113S-adenosyl-L-methionine bindingBy similarity2

Sequence similaritiesi

Belongs to the diphthine synthase family.Curated

Phylogenomic databases

eggNOGiKOG3123. Eukaryota.
COG1798. LUCA.
GeneTreeiENSGT00390000010568.
HOGENOMiHOG000205302.
HOVERGENiHBG044879.
InParanoidiQ9CWQ0.
KOiK00586.
OMAiMTANEGL.
OrthoDBiEOG091G0EKA.
TreeFamiTF105603.

Family and domain databases

CDDicd11647. Diphthine_synthase. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01084. Diphthine_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR004551. Dphthn_synthase.
[Graphical view]
PANTHERiPTHR10882:SF0. PTHR10882:SF0. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036432. Diphthine_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00522. dph5. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9CWQ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLYLIGLGLG DAKDITVKGL EVVRRCSRVY LEAYTSVLTV GKEALEEFYG
60 70 80 90 100
RKLILADREE VEQEADNIFK DADVSDVAFL VVGDPFGATT HSDLILRATK
110 120 130 140 150
LGIPYQVIHN ASIMNAVGCC GLQLYRFGET VSIVFWTDTW RPESFFDKVK
160 170 180 190 200
RNRANGMHTL CLLDIKVKEQ SLENLIRGRK IYEPPRYMSV NQAAQQLLEI
210 220 230 240 250
VQNHRARGEE PAITEETLCV GLARVGAEDQ KIAAGTLQQM CTVSLGEPLH
260 270 280
SLVITGGNLH PLEMEMLSLF SIPESQSTDG L
Length:281
Mass (Da):31,218
Last modified:October 3, 2012 - v2
Checksum:i28AEC5E6ABBC3C1F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16T → K in BAB26968 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010475 mRNA. Translation: BAB26968.1.
AC131113 Genomic DNA. No translation available.
CH466532 Genomic DNA. Translation: EDL12395.1.
BC060372 mRNA. Translation: AAH60372.1.
CCDSiCCDS17782.1.
RefSeqiNP_081469.2. NM_027193.2.
UniGeneiMm.5915.

Genome annotation databases

EnsembliENSMUST00000043342; ENSMUSP00000043730; ENSMUSG00000033554.
ENSMUST00000189799; ENSMUSP00000140958; ENSMUSG00000033554.
GeneIDi69740.
KEGGimmu:69740.
UCSCiuc008rbs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010475 mRNA. Translation: BAB26968.1.
AC131113 Genomic DNA. No translation available.
CH466532 Genomic DNA. Translation: EDL12395.1.
BC060372 mRNA. Translation: AAH60372.1.
CCDSiCCDS17782.1.
RefSeqiNP_081469.2. NM_027193.2.
UniGeneiMm.5915.

3D structure databases

ProteinModelPortaliQ9CWQ0.
SMRiQ9CWQ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043730.

PTM databases

PhosphoSitePlusiQ9CWQ0.

Proteomic databases

EPDiQ9CWQ0.
MaxQBiQ9CWQ0.
PaxDbiQ9CWQ0.
PeptideAtlasiQ9CWQ0.
PRIDEiQ9CWQ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043342; ENSMUSP00000043730; ENSMUSG00000033554.
ENSMUST00000189799; ENSMUSP00000140958; ENSMUSG00000033554.
GeneIDi69740.
KEGGimmu:69740.
UCSCiuc008rbs.1. mouse.

Organism-specific databases

CTDi51611.
MGIiMGI:1916990. Dph5.

Phylogenomic databases

eggNOGiKOG3123. Eukaryota.
COG1798. LUCA.
GeneTreeiENSGT00390000010568.
HOGENOMiHOG000205302.
HOVERGENiHBG044879.
InParanoidiQ9CWQ0.
KOiK00586.
OMAiMTANEGL.
OrthoDBiEOG091G0EKA.
TreeFamiTF105603.

Enzyme and pathway databases

UniPathwayiUPA00559.
ReactomeiR-MMU-5358493. Synthesis of diphthamide-EEF2.

Miscellaneous databases

PROiQ9CWQ0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033554.
CleanExiMM_DPH5.
ExpressionAtlasiQ9CWQ0. baseline and differential.
GenevisibleiQ9CWQ0. MM.

Family and domain databases

CDDicd11647. Diphthine_synthase. 1 hit.
Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
HAMAPiMF_01084. Diphthine_synth. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR004551. Dphthn_synthase.
[Graphical view]
PANTHERiPTHR10882:SF0. PTHR10882:SF0. 1 hit.
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036432. Diphthine_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR00522. dph5. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDPH5_MOUSE
AccessioniPrimary (citable) accession number: Q9CWQ0
Secondary accession number(s): Q6PAC5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: October 3, 2012
Last modified: November 2, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.