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Q9CWQ0

- DPH5_MOUSE

UniProt

Q9CWQ0 - DPH5_MOUSE

Protein

Diphthine synthase

Gene

Dph5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (03 Oct 2012)
      Previous versions | rss
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    Functioni

    S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EFE2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.1 Publication

    Catalytic activityi

    3 S-adenosyl-L-methionine + 2-(3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] = 3 S-adenosyl-L-homocysteine + 2-(3-carboxy-3-(trimethylammonio)propyl)-L-histidine-[translation elongation factor 2].

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei9 – 91S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei84 – 841S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei87 – 871S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
    Binding sitei163 – 1631S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei225 – 2251S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei250 – 2501S-adenosyl-L-methionine; via carbonyl oxygenBy similarity

    GO - Molecular functioni

    1. diphthine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00559.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diphthine synthase (EC:2.1.1.98)
    Alternative name(s):
    Diphthamide biosynthesis methyltransferase
    Gene namesi
    Name:Dph5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:1916990. Dph5.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 281281Diphthine synthasePRO_0000156134Add
    BLAST

    Proteomic databases

    PaxDbiQ9CWQ0.
    PRIDEiQ9CWQ0.

    PTM databases

    PhosphoSiteiQ9CWQ0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9CWQ0.
    CleanExiMM_DPH5.
    GenevestigatoriQ9CWQ0.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CWQ0.
    SMRiQ9CWQ0. Positions 1-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni112 – 1132S-adenosyl-L-methionine bindingBy similarity

    Sequence similaritiesi

    Belongs to the diphthine synthase family.Curated

    Phylogenomic databases

    eggNOGiCOG1798.
    GeneTreeiENSGT00390000010568.
    HOGENOMiHOG000205302.
    HOVERGENiHBG044879.
    InParanoidiQ9CWQ0.
    KOiK00586.
    OMAiANCPAVA.
    TreeFamiTF105603.

    Family and domain databases

    Gene3Di3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    HAMAPiMF_01084. Diphthine_synth.
    InterProiIPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR004551. Dphthn_synthase.
    [Graphical view]
    PfamiPF00590. TP_methylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036432. Diphthine_synth. 1 hit.
    SUPFAMiSSF53790. SSF53790. 1 hit.
    TIGRFAMsiTIGR00522. dph5. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9CWQ0-1 [UniParc]FASTAAdd to Basket

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    MLYLIGLGLG DAKDITVKGL EVVRRCSRVY LEAYTSVLTV GKEALEEFYG    50
    RKLILADREE VEQEADNIFK DADVSDVAFL VVGDPFGATT HSDLILRATK 100
    LGIPYQVIHN ASIMNAVGCC GLQLYRFGET VSIVFWTDTW RPESFFDKVK 150
    RNRANGMHTL CLLDIKVKEQ SLENLIRGRK IYEPPRYMSV NQAAQQLLEI 200
    VQNHRARGEE PAITEETLCV GLARVGAEDQ KIAAGTLQQM CTVSLGEPLH 250
    SLVITGGNLH PLEMEMLSLF SIPESQSTDG L 281
    Length:281
    Mass (Da):31,218
    Last modified:October 3, 2012 - v2
    Checksum:i28AEC5E6ABBC3C1F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161T → K in BAB26968. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK010475 mRNA. Translation: BAB26968.1.
    AC131113 Genomic DNA. No translation available.
    CH466532 Genomic DNA. Translation: EDL12395.1.
    BC060372 mRNA. Translation: AAH60372.1.
    CCDSiCCDS17782.1.
    RefSeqiNP_081469.2. NM_027193.2.
    UniGeneiMm.5915.

    Genome annotation databases

    EnsembliENSMUST00000043342; ENSMUSP00000043730; ENSMUSG00000033554.
    GeneIDi69740.
    KEGGimmu:69740.
    UCSCiuc008rbs.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK010475 mRNA. Translation: BAB26968.1 .
    AC131113 Genomic DNA. No translation available.
    CH466532 Genomic DNA. Translation: EDL12395.1 .
    BC060372 mRNA. Translation: AAH60372.1 .
    CCDSi CCDS17782.1.
    RefSeqi NP_081469.2. NM_027193.2.
    UniGenei Mm.5915.

    3D structure databases

    ProteinModelPortali Q9CWQ0.
    SMRi Q9CWQ0. Positions 1-270.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9CWQ0.

    Proteomic databases

    PaxDbi Q9CWQ0.
    PRIDEi Q9CWQ0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043342 ; ENSMUSP00000043730 ; ENSMUSG00000033554 .
    GeneIDi 69740.
    KEGGi mmu:69740.
    UCSCi uc008rbs.1. mouse.

    Organism-specific databases

    CTDi 51611.
    MGIi MGI:1916990. Dph5.

    Phylogenomic databases

    eggNOGi COG1798.
    GeneTreei ENSGT00390000010568.
    HOGENOMi HOG000205302.
    HOVERGENi HBG044879.
    InParanoidi Q9CWQ0.
    KOi K00586.
    OMAi ANCPAVA.
    TreeFami TF105603.

    Enzyme and pathway databases

    UniPathwayi UPA00559 .

    Miscellaneous databases

    NextBioi 330226.
    PROi Q9CWQ0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9CWQ0.
    CleanExi MM_DPH5.
    Genevestigatori Q9CWQ0.

    Family and domain databases

    Gene3Di 3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    HAMAPi MF_01084. Diphthine_synth.
    InterProi IPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR004551. Dphthn_synthase.
    [Graphical view ]
    Pfami PF00590. TP_methylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036432. Diphthine_synth. 1 hit.
    SUPFAMi SSF53790. SSF53790. 1 hit.
    TIGRFAMsi TIGR00522. dph5. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryonic stem cell.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Olfactory epithelium.
    5. "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2."
      Liu S., Milne G.T., Kuremsky J.G., Fink G.R., Leppla S.H.
      Mol. Cell. Biol. 24:9487-9497(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiDPH5_MOUSE
    AccessioniPrimary (citable) accession number: Q9CWQ0
    Secondary accession number(s): Q6PAC5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 10, 2003
    Last sequence update: October 3, 2012
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3