Q9CWL2 (CASZ1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc finger protein castor homolog 1

Alternative name(s):
Castor-related protein

Gene names

Name:	Casz1
Synonyms:	Cst, D4Ertd432e, Kiaa3026

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1761 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable transcription factor By similarity.
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in the brain stem and the thalamencephalon. Ref.4 Ref.5
Developmental stage	First expressed at E8.0 in the developing heart and throughout development. By E8.5, it is expressed in the lateral neural folds of the hindbrain and extends anteriorly and posteriorly to eventually cover the dorsal neural tube from the isthmus to its caudal end. From E9.5, it is expressed in the dorsomedial telencephalon. In the hindbrain, it is confined to trigeminal motor neurons and to migrating facial branchiomotor neurons. In the peripheral nervous system, it is expressed in cranial and in dorsal root ganglia. Also expressed in the developing eye and in the nasal placode. Ref.4
Induction	Up-regulated during myoblast differentiation. Ref.5
Sequence similarities	Contains 8 C2H2-type zinc fingers.
Sequence caution	The sequence BAB27027.1 differs from that shown. Reason: Frameshift at position 946.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9CWL2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Gene prediction based on similarity to human ortholog.

Isoform 2 (identifier: Q9CWL2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1166-1166: N → K
     1167-1761: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1761	1761	Zinc finger protein castor homolog 1		PRO_0000046913
Regions
	Zinc finger	550 – 574	25	C2H2-type 1
	Zinc finger	609 – 633	25	C2H2-type 2
	Zinc finger	667 – 691	25	C2H2-type 3
	Zinc finger	1031 – 1055	25	C2H2-type 4
	Zinc finger	1300 – 1324	25	C2H2-type 5
	Zinc finger	1457 – 1481	25	C2H2-type 6
	Zinc finger	1515 – 1537	23	C2H2-type 7
	Zinc finger	1571 – 1595	25	C2H2-type 8
	Compositional bias	383 – 416	34	Pro-rich
	Compositional bias	1080 – 1143	64	Pro-rich
	Compositional bias	1669 – 1681	13	Poly-Thr
	Compositional bias	1702 – 1729	28	Asp-rich
Amino acid modifications
	Modified residue	981	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1166	1	N → K in isoform 2.		VSP_027095
	Alternative sequence	1167 – 1761	595	Missing in isoform 2.		VSP_027096
Experimental info
	Sequence conflict	884	1	A → S in BAD32619. Ref.2
	Sequence conflict	912	1	Q → E in BAB27027. Ref.3
	Sequence conflict	1087	1	L → P in BAB27027. Ref.3
	Sequence conflict	1087	1	L → P in BAD32619. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 24, 2007. Version 3. Checksum: C0C1959D68D62D50	FASTA	1,761	191,188
	10 20 30 40 50 60 MDLGTAESTR CTDPPAGKPP MAAKRKGGLK LNAICAKLSR QVVVEKGAEA GSQAEGSPLH 70 80 90 100 110 120 PRDKERSGPE SGVSRAPRSE EDKRRAVIEK WVNGEYCEDP APTPVLGRIA RDQELPPEGV 130 140 150 160 170 180 YMVQPQGCSD EEDHAEEPSK DNSVLEEKES DGTASKDDSG PSTRQASGET SSLRDYAAST 190 200 210 220 230 240 MTEFLGMFGY DDQNTRDELA KKISFEKPHA GSTPEVAASS MLPSSEDTLS KRARFSKYEE 250 260 270 280 290 300 YIRKLKAGEQ LPWPAHGSKA EDRAGKEVVG PLPSLRLPSN TAHLETKATI LPLPSHSSVQ 310 320 330 340 350 360 MQNLVARASK YDFFIHKLKT GENLRPQNGS TYKKPSKYDL ENVKYLHLFK PGEGSPDMGG 370 380 390 400 410 420 AIAFKTGKVG RPSKYDVRGI QKPGPTKIPP APSLVPTPLT NVPSAPSTPG PGPEPPASLS 430 440 450 460 470 480 FNTPEYLKST FSKTDSITTG TVSTVKNGLP TDKPAVTEDV NIYQKYIARF SGSQHCGHIH 490 500 510 520 530 540 CAYQYREHYH CLDPECNYQR FTSKQDVIRH YNMHKKRDNS LQHGFMRFSP LDDCSVYYHG 550 560 570 580 590 600 CHLNGKSTHY HCMQVGCNKV YTSTSDVMTH ENFHKKNTQL INDGFQRFRA TEDCGTADCQ 610 620 630 640 650 660 FYGQKTTHFH CRRPGCTFTF KNKCDIEKHK SYHIKDDAYA KDGFKKFYKY EECKYEGCMY 670 680 690 700 710 720 SKATNHFHCI RAGCGFTFTS TSQMTSHKRK HERRHIRSSG ALGLPASLLG AKDTEHEESS 730 740 750 760 770 780 NDDLVDFSAL SSKNSSLSAS PTSQQSSASL AAAAAATTAE AIPSATKPPN SKMAGLLPQG 790 800 810 820 830 840 LSGSIPLALA LSNSGLPTTT PYFPLLPNRG SASLPVGSPG LLGSMSSGAT TSATPDMPAL 850 860 870 880 890 900 MASRAGDSAP TAATSLSVPP ASIIERISAS KGLISPMMAR LAAAALKPSA TFDPGSGQQP 910 920 930 940 950 960 TPTKFPQAQV KQEPDSAGTP GPHEASQDRS LDLTVKDPSN ESNGHAVSAN SSLLSSLMNK 970 980 990 1000 1010 1020 MSQGNPSLES FLSIKTEAEG SPAGEPSPFL GKAVKALVQE KLSEPWKVYL RRFGTKDFCD 1030 1040 1050 1060 1070 1080 AQCDFLHKAH FHCVVEECGA LFSTLDGAIK HANFHFRTEG GTAKGTPEAS FPTSAAETKP 1090 1100 1110 1120 1130 1140 PLAPSSLPAP PGTMVAGSSL EGPAPSPVSV PSTPTLLAWK QLASTIPQMP QIPSSVPHLP 1150 1160 1170 1180 1190 1200 TSPLATTSLE SAKPQVKPGF LQFQDNDPCL ATDCKYASKF HFHCLFGNCK YVCKTSGKAE 1210 1220 1230 1240 1250 1260 SHCLDHINPS NSLVNVRDQF AYYSLQCLCP NQHCEFRMRG HYHCLRTGCY FVTNITTKLP 1270 1280 1290 1300 1310 1320 WHIKKHEKAE RRAANGFKYF TKREECGRLG CKYNQVNSHF HCIREGCQFS FLLKHQMTSH 1330 1340 1350 1360 1370 1380 ARKHMRRMLG KNFDRVPPSQ GPPSLMDAET DEGMDYTGCS PGAASSESST MDRSCSSTPV 1390 1400 1410 1420 1430 1440 GNESTAAGNT ISMPTASGAK KRFWIIEDMS PFGKRRKTAS SRKMLDEGMM LEGFRRFDLY 1450 1460 1470 1480 1490 1500 EDCKDTACQF SLKVTHYHCT RENCGYKFCG RTHMYKHAQH HDRVDNLVLD DFKRFKASLS 1510 1520 1530 1540 1550 1560 CHFADCPFSG TSTHFHCLRC RFRCTDSTKV TAHRKHHGKQ DVISAAGFCQ FSSSADCAVP 1570 1580 1590 1600 1610 1620 DCKYKLKCSH FHCTYPGCRH TVVGMSQMDS HKRKHEKQER GEPPAASPGA PVNLDGSLTL 1630 1640 1650 1660 1670 1680 AAEQGSLLFL QTAAAGLGLL GDTGDPGPPV TASGTRDGPA APTPAAAATT TTTTTATATA 1690 1700 1710 1720 1730 1740 TAGESSQEDD EELELPEEEA EDDDEDDDEE DDDDEDDDDD DDDEDLRTDS EESLPEAAGE 1750 1760 AGARTPLAAL GGPGPAPTAA S
	Isoform 2 [UniParc]. Checksum: 4804ED3BB3A77BD2	FASTA	1,166	125,596
	10 20 30 40 50 60 MDLGTAESTR CTDPPAGKPP MAAKRKGGLK LNAICAKLSR QVVVEKGAEA GSQAEGSPLH 70 80 90 100 110 120 PRDKERSGPE SGVSRAPRSE EDKRRAVIEK WVNGEYCEDP APTPVLGRIA RDQELPPEGV 130 140 150 160 170 180 YMVQPQGCSD EEDHAEEPSK DNSVLEEKES DGTASKDDSG PSTRQASGET SSLRDYAAST 190 200 210 220 230 240 MTEFLGMFGY DDQNTRDELA KKISFEKPHA GSTPEVAASS MLPSSEDTLS KRARFSKYEE 250 260 270 280 290 300 YIRKLKAGEQ LPWPAHGSKA EDRAGKEVVG PLPSLRLPSN TAHLETKATI LPLPSHSSVQ 310 320 330 340 350 360 MQNLVARASK YDFFIHKLKT GENLRPQNGS TYKKPSKYDL ENVKYLHLFK PGEGSPDMGG 370 380 390 400 410 420 AIAFKTGKVG RPSKYDVRGI QKPGPTKIPP APSLVPTPLT NVPSAPSTPG PGPEPPASLS 430 440 450 460 470 480 FNTPEYLKST FSKTDSITTG TVSTVKNGLP TDKPAVTEDV NIYQKYIARF SGSQHCGHIH 490 500 510 520 530 540 CAYQYREHYH CLDPECNYQR FTSKQDVIRH YNMHKKRDNS LQHGFMRFSP LDDCSVYYHG 550 560 570 580 590 600 CHLNGKSTHY HCMQVGCNKV YTSTSDVMTH ENFHKKNTQL INDGFQRFRA TEDCGTADCQ 610 620 630 640 650 660 FYGQKTTHFH CRRPGCTFTF KNKCDIEKHK SYHIKDDAYA KDGFKKFYKY EECKYEGCMY 670 680 690 700 710 720 SKATNHFHCI RAGCGFTFTS TSQMTSHKRK HERRHIRSSG ALGLPASLLG AKDTEHEESS 730 740 750 760 770 780 NDDLVDFSAL SSKNSSLSAS PTSQQSSASL AAAAAATTAE AIPSATKPPN SKMAGLLPQG 790 800 810 820 830 840 LSGSIPLALA LSNSGLPTTT PYFPLLPNRG SASLPVGSPG LLGSMSSGAT TSATPDMPAL 850 860 870 880 890 900 MASRAGDSAP TAATSLSVPP ASIIERISAS KGLISPMMAR LAAAALKPSA TFDPGSGQQP 910 920 930 940 950 960 TPTKFPQAQV KQEPDSAGTP GPHEASQDRS LDLTVKDPSN ESNGHAVSAN SSLLSSLMNK 970 980 990 1000 1010 1020 MSQGNPSLES FLSIKTEAEG SPAGEPSPFL GKAVKALVQE KLSEPWKVYL RRFGTKDFCD 1030 1040 1050 1060 1070 1080 AQCDFLHKAH FHCVVEECGA LFSTLDGAIK HANFHFRTEG GTAKGTPEAS FPTSAAETKP 1090 1100 1110 1120 1130 1140 PLAPSSLPAP PGTMVAGSSL EGPAPSPVSV PSTPTLLAWK QLASTIPQMP QIPSSVPHLP 1150 1160 TSPLATTSLE SAKPQVKPGF LQFQDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[2]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 286-1761 (ISOFORM 2). Tissue: Pancreatic islet.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 912-1761 (ISOFORM 2). Strain: C57BL/6J. Tissue: Embryonic stem cell.
[4]	"Cst, a novel mouse gene related to Drosophila Castor, exhibits dynamic expression patterns during neurogenesis and heart development." Vacalla C.M.H., Theil T. Mech. Dev. 118:265-268(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]	"Molecular cloning and characterization of human Castor, a novel human gene upregulated during cell differentiation." Liu Z., Yang X., Tan F., Cullion K., Thiele C.J. Biochem. Biophys. Res. Commun. 344:834-844(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL607145 Genomic DNA. Translation: CAM18127.1. AL611967 Genomic DNA. No translation available. AK173341 mRNA. Translation: BAD32619.1. AK010559 mRNA. Translation: BAB27027.1. Frameshift.
IPI	IPI00342841. IPI00347203.
RefSeq	NP_081471.2. NM_027195.2.
UniGene	Mm.233879.
3D structure databases
ProteinModelPortal	Q9CWL2.
SMR	Q9CWL2. Positions 550-577, 610-636, 1571-1598.
ModBase	Search...
PTM databases
PhosphoSite	Q9CWL2.
Proteomic databases
PaxDb	Q9CWL2.
PRIDE	Q9CWL2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000094464; ENSMUSP00000092035; ENSMUSG00000028977.
GeneID	69743.
KEGG	mmu:69743.
UCSC	uc008vvl.1. mouse.
Organism-specific databases
CTD	54897.
MGI	MGI:1196251. Casz1.
Rouge	Search...
Phylogenomic databases
eggNOG	NOG324200.
GeneTree	ENSGT00390000008187.
HOVERGEN	HBG080122.
InParanoid	Q9CWL2.
OMA	TAEGTRC.
Gene expression databases
ArrayExpress	Q9CWL2.
Bgee	Q9CWL2.
CleanEx	MM_CASZ1.
Genevestigator	Q9CWL2.
GermOnline	ENSMUSG00000028977. Mus musculus.
Family and domain databases
Gene3D	1.25.10.10. 1 hit.
InterPro	IPR011989. ARM-like. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. [Graphical view]
SMART	SM00355. ZnF_C2H2. 11 hits. [Graphical view]
PROSITE	PS00028. ZINC_FINGER_C2H2_1. 9 hits. PS50157. ZINC_FINGER_C2H2_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	330234.
SOURCE	Search...

Entry information

Entry name

CASZ1_MOUSE

Accession

Primary (citable) accession number: Q9CWL2
Secondary accession number(s): A2A8A1, Q69Z25

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	July 24, 2007
Last modified:	May 1, 2013
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program