Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

Gene

B3gat1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.By similarity

Catalytic activityi

UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine.By similarity

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei122UDP-glucuronateBy similarity1
Binding sitei165UDP-glucuronateBy similarity1
Binding sitei170UDP-glucuronateBy similarity1
Metal bindingi197ManganeseBy similarity1
Active sitei284Proton donor/acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi91 – 93UDP-glucuronate bindingBy similarity3
Nucleotide bindingi195 – 197UDP-glucuronate bindingBy similarity3
Nucleotide bindingi311 – 313UDP-glucuronate bindingBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1By similarity (EC:2.4.1.135By similarity)
Alternative name(s):
Beta-1,3-glucuronyltransferase 1
Glucuronosyltransferase P
Short name:
GlcAT-PBy similarity
UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
Short name:
GlcUAT-P
Gene namesi
Name:B3gat1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1924148. B3gat1.

Subcellular locationi

Isoform 1 :
  • Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
  • Secreted By similarity
Isoform 2 :
  • Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity
  • Endoplasmic reticulum membrane By similarity
  • Secreted By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini28 – 334LumenalSequence analysisAdd BLAST307

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001951681 – 334Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1Add BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei103PhosphothreonineBy similarity1
Modified residuei108PhosphothreonineBy similarity1
Glycosylationi140N-linked (GlcNAc...)Sequence analysis1
Glycosylationi184N-linked (GlcNAc...)Sequence analysis1
Glycosylationi303N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9CW73.
PRIDEiQ9CW73.

PTM databases

PhosphoSitePlusiQ9CW73.

Expressioni

Gene expression databases

BgeeiENSMUSG00000045994.

Interactioni

Subunit structurei

Homodimer. Interacts with SAR1A.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei228Interaction with galactose moiety of substrate glycoproteinBy similarity1
Sitei321Interaction with galactose moiety of substrate glycoproteinBy similarity1

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125700.

Structurei

3D structure databases

ProteinModelPortaliQ9CW73.
SMRiQ9CW73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni3 – 5Essential for transport from endoplasmic reticulum to Golgi apparatus and interaction with SAR1ABy similarity3
Regioni245 – 254Interaction with galactose moiety of substrate glycoproteinBy similarity10

Sequence similaritiesi

Belongs to the glycosyltransferase 43 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
GeneTreeiENSGT00390000017640.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiQ9CW73.
KOiK00735.
OMAiKDEGSDP.
TreeFamiTF313522.

Family and domain databases

CDDicd00218. GlcAT-I. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CW73-1) [UniParc]FASTAAdd to basket
Also known as: sGlcAT-PBy similarity

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPKRRDILAI VLIVLPWTLL ITVWHQSSLA PLLAVHKDEG SDPRHEAPPG
60 70 80 90 100
ADPREYCMSD RDIVEVVRTE YVYTRPPPWS DTLPTIHVVT PTYSRPVQKA
110 120 130 140 150
ELTRMANTLL HVPNLHWLVV EDAPRRTPLT ARLLRDTGLN YTHLHVETPR
160 170 180 190 200
NYKLRGDARD PRIPRGTMQR NLALRWLRET FPRNSTQPGV VYFADDDNTY
210 220 230 240 250
SLELFEEMRS TRRVSVWPVA FVGGLRYEAP RVNGAGKVVG WKTVFDPHRP
260 270 280 290 300
FAIDMAGFAV NLRLILQRSQ AYFKLRGVKG GYQESSLLRE LVTLNDLEPK
310 320 330
AANCTKILVW HTRTEKPVLV NEGKKGFTDP SVEI
Length:334
Mass (Da):38,237
Last modified:October 5, 2016 - v3
Checksum:i2C9AD8A19AA11379
GO
Isoform 2 (identifier: Q9CW73-2) [UniParc]FASTAAdd to basket
Also known as: lGlcAT-PBy similarity

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGNEELWVQPALEM

Show »
Length:347
Mass (Da):39,735
Checksum:iCD4997A7E0BEDD12
GO

Sequence cautioni

The sequence BAC31996 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti121E → G in BAC31996 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0585391M → MGNEELWVQPALEM in isoform 2. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055781 mRNA. Translation: BAB85676.1.
AK003020 mRNA. Translation: BAB22514.1.
AK044599 mRNA. Translation: BAC31996.1. Different initiation.
AK082739 mRNA. Translation: BAC38594.1.
AC109831 Genomic DNA. No translation available.
AC161424 Genomic DNA. No translation available.
BC034655 mRNA. Translation: AAH34655.1.
CCDSiCCDS22936.1. [Q9CW73-1]
CCDS80970.1. [Q9CW73-2]
PIRiJC7828.
RefSeqiNP_001297695.1. NM_001310766.1. [Q9CW73-2]
NP_084068.1. NM_029792.1. [Q9CW73-1]
XP_006510737.1. XM_006510674.1. [Q9CW73-1]
UniGeneiMm.218788.

Genome annotation databases

EnsembliENSMUST00000115269; ENSMUSP00000110924; ENSMUSG00000045994. [Q9CW73-2]
ENSMUST00000159799; ENSMUSP00000124438; ENSMUSG00000045994. [Q9CW73-2]
ENSMUST00000160899; ENSMUSP00000124067; ENSMUSG00000045994. [Q9CW73-2]
ENSMUST00000161115; ENSMUSP00000125700; ENSMUSG00000045994. [Q9CW73-1]
GeneIDi76898.
KEGGimmu:76898.
UCSCiuc009opo.1. mouse.
uc009opp.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055781 mRNA. Translation: BAB85676.1.
AK003020 mRNA. Translation: BAB22514.1.
AK044599 mRNA. Translation: BAC31996.1. Different initiation.
AK082739 mRNA. Translation: BAC38594.1.
AC109831 Genomic DNA. No translation available.
AC161424 Genomic DNA. No translation available.
BC034655 mRNA. Translation: AAH34655.1.
CCDSiCCDS22936.1. [Q9CW73-1]
CCDS80970.1. [Q9CW73-2]
PIRiJC7828.
RefSeqiNP_001297695.1. NM_001310766.1. [Q9CW73-2]
NP_084068.1. NM_029792.1. [Q9CW73-1]
XP_006510737.1. XM_006510674.1. [Q9CW73-1]
UniGeneiMm.218788.

3D structure databases

ProteinModelPortaliQ9CW73.
SMRiQ9CW73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000125700.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

PTM databases

PhosphoSitePlusiQ9CW73.

Proteomic databases

PaxDbiQ9CW73.
PRIDEiQ9CW73.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000115269; ENSMUSP00000110924; ENSMUSG00000045994. [Q9CW73-2]
ENSMUST00000159799; ENSMUSP00000124438; ENSMUSG00000045994. [Q9CW73-2]
ENSMUST00000160899; ENSMUSP00000124067; ENSMUSG00000045994. [Q9CW73-2]
ENSMUST00000161115; ENSMUSP00000125700; ENSMUSG00000045994. [Q9CW73-1]
GeneIDi76898.
KEGGimmu:76898.
UCSCiuc009opo.1. mouse.
uc009opp.1. mouse.

Organism-specific databases

CTDi27087.
MGIiMGI:1924148. B3gat1.

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
GeneTreeiENSGT00390000017640.
HOGENOMiHOG000261693.
HOVERGENiHBG050650.
InParanoidiQ9CW73.
KOiK00735.
OMAiKDEGSDP.
TreeFamiTF313522.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.

Miscellaneous databases

PROiQ9CW73.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045994.

Family and domain databases

CDDicd00218. GlcAT-I. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB3GA1_MOUSE
AccessioniPrimary (citable) accession number: Q9CW73
Secondary accession number(s): Q6PIG8, Q8BXN8, Q8R531
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: October 5, 2016
Last modified: November 30, 2016
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.