Reviewed, UniProtKB/Swiss-Prot Q9CW73 (B3GA1_MOUSE)

Names and origin

Protein names

Recommended name:
    Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
    EC=2.4.1.135
Alternative name(s):
    Beta-1,3-glucuronyltransferase 1
    Glucuronosyltransferase-P
      Short name=GlcAT-P
    UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase
      Short name=GlcUAT-P

Gene names

Name:

B3gat1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group By similarity.
Catalytic activity	UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer Potential.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 43 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity Inferred from direct assay. Source: MGI galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1		PRO_0000195168
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 27	21	Signal-anchor for type II membrane protein Potential
	Topological domain	28 – 334	307	Lumenal Potential
Sites
	Active site	284	1	Proton acceptor By similarity
	Metal binding	197	1	Manganese By similarity
Amino acid modifications
	Glycosylation	140	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	121	1	G → E in BAB22514. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9CW73-1 [UniParc]. Last modified April 26, 2005. Version 2. Checksum: 6BDAF9F6DA80A40C	FASTA	334	38,165
	10 20 30 40 50 60 MPKRRDILAI VLIVLPWTLL ITVWHQSSLA PLLAVHKDEG SDPRHEAPPG ADPREYCMSD 70 80 90 100 110 120 RDIVEVVRTE YVYTRPPPWS DTLPTIHVVT PTYSRPVQKA ELTRMANTLL HVPNLHWLVV 130 140 150 160 170 180 GDAPRRTPLT ARLLRDTGLN YTHLHVETPR NYKLRGDARD PRIPRGTMQR NLALRWLRET 190 200 210 220 230 240 FPRNSTQPGV VYFADDDNTY SLELFEEMRS TRRVSVWPVA FVGGLRYEAP RVNGAGKVVG 250 260 270 280 290 300 WKTVFDPHRP FAIDMAGFAV NLRLILQRSQ AYFKLRGVKG GYQESSLLRE LVTLNDLEPK 310 320 330 AANCTKILVW HTRTEKPVLV NEGKKGFTDP SVEI

References

[1]

"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Brain and Retina.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK003020 mRNA. Translation: BAB22514.1. AK044599 mRNA. Translation: BAC31996.1. Different initiation.
IPI	IPI00405708.
UniGene	Mm.218788
3D structure databases
HSSP	HSSP built from PDB template 1KWS based on UniProtKB O94766.
SMR	Q9CW73. Positions 83-334.
ModBase	Search...
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Genome annotation databases
Ensembl	ENSMUSG00000045994. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1924148. B3gat1.
Phylogenomic databases
HOVERGEN	Q9CW73.
Enzyme and pathway databases
BRENDA	2.4.1.135. 244.
Gene expression databases
ArrayExpress	Q9CW73.
Bgee	Q9CW73.
GermOnline	ENSMUSG00000045994. Mus musculus.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
PANTHER	PTHR10896. Glyco_trans_43. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

B3GA1_MOUSE

Accession

Primary (citable) accession number: Q9CW73
Secondary accession number(s): Q8BXN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	April 26, 2005
Last modified:	June 16, 2009
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)