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Protein

Ribonucleoprotein PTB-binding 1

Gene

Raver1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cooperates with PTBP1 to modulate regulated alternative splicing events. Promotes exon skipping. Cooperates with PTBP1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoprotein PTB-binding 1
Alternative name(s):
Protein raver-1
Gene namesi
Name:Raver1
Synonyms:Kiaa1978
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1919016. Raver1.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

  • Note: Nuclear, in perinucleolar structures. Shuttles between nucleus and cytoplasm. Cytoplasm, at focal contacts and cell-cell contacts. Associated with myotubes during muscle differentiation.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 748747Ribonucleoprotein PTB-binding 1PRO_0000081488Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCombined sources
Modified residuei14 – 141PhosphoserineCombined sources
Modified residuei469 – 4691PhosphothreonineCombined sources
Modified residuei480 – 4801PhosphoserineBy similarity
Modified residuei576 – 5761PhosphoserineCombined sources
Modified residuei626 – 6261PhosphoserineCombined sources
Modified residuei630 – 6301PhosphoserineCombined sources
Modified residuei716 – 7161PhosphoserineCombined sources
Modified residuei720 – 7201PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9CW46.
MaxQBiQ9CW46.
PaxDbiQ9CW46.
PRIDEiQ9CW46.

PTM databases

iPTMnetiQ9CW46.
PhosphoSiteiQ9CW46.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

BgeeiQ9CW46.
CleanExiMM_RAVER1.
GenevisibleiQ9CW46. MM.

Interactioni

Subunit structurei

Interacts with PTBP1, RAVER2, VCL and ACTN1. Part of a complex containing RAVER1, VCL and ACTN1.2 Publications

Protein-protein interaction databases

BioGridi214910. 7 interactions.
DIPiDIP-44609N.
IntActiQ9CW46. 4 interactions.
MINTiMINT-1707126.
STRINGi10090.ENSMUSP00000111150.

Structurei

Secondary structure

1
748
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi61 – 644Combined sources
Helixi72 – 787Combined sources
Turni79 – 813Combined sources
Beta strandi84 – 907Combined sources
Turni91 – 944Combined sources
Beta strandi95 – 1028Combined sources
Helixi103 – 11311Combined sources
Beta strandi116 – 1205Combined sources
Beta strandi122 – 1276Combined sources
Beta strandi684 – 6863Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WI6NMR-A61-135[»]
3ZZYX-ray1.40C/D496-507[»]
3ZZZX-ray1.55C/D680-692[»]
ProteinModelPortaliQ9CW46.
SMRiQ9CW46. Positions 39-316.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9CW46.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini59 – 13072RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini132 – 21079RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini221 – 29979RRM 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni307 – 40195Interaction with PTBP1Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi45 – 6016Nuclear localization signalSequence analysisAdd
BLAST
Motifi743 – 7464Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi432 – 629198Pro-richAdd
BLAST

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IJBP. Eukaryota.
ENOG410ZP6I. LUCA.
GeneTreeiENSGT00390000006046.
HOGENOMiHOG000253941.
HOVERGENiHBG059515.
InParanoidiQ9CW46.
OMAiHGPSRHK.
OrthoDBiEOG741Z3J.
PhylomeDBiQ9CW46.
TreeFamiTF331660.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CW46-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAADVSVTHR PPLSPEAEAE AETPETVDRR APEQELPPLD PEEIRKRLEH
60 70 80 90 100
TERQFRNRRK ILIRGLPGDV TNQEVHDLLS DYELKYCFVD KYKGTAFVTL
110 120 130 140 150
LNGEQAEAAI NTFHQSRLRE RELSVQLQPT DALLCVANLP PSLTQAQFEE
160 170 180 190 200
LVRPFGSLER CFLVYSERTG HSKGYGFAEY MKKDSAARAK SDLLGKPLGP
210 220 230 240 250
RTLYVHWTDA GQLTPALLHS RCLCVDHLPP GFSDVDALRR ALSVVYTPTF
260 270 280 290 300
CQLACGQDGQ LKGFAVLEYE TAEMAEAAQE RADGQALGDS HLRVSFCAPG
310 320 330 340 350
PPGRSMLAAL IAAQATALNR GKGLLPEPNL LQLLNNLGPS ASLQLLLNPL
360 370 380 390 400
LHGGASGKQG LLGAPPAMPL LSGPALSTAL LQLALQSQSQ NQSQGQKKPG
410 420 430 440 450
ILGDSPLGTL QAGAQPSNSL LGELSAGGGL APELPPRRGK PQPLLPPLLG
460 470 480 490 500
PSGGDREPMG LGPPATQLTP PPAPVGLRGS NHRGLPKDSG PLPTPPGVSL
510 520 530 540 550
LGEPPKDYRI PLNPYLNLHS LLPSSNLAGK ETRGWGGSGR GRRPAEPPLP
560 570 580 590 600
SPAVPGGGSG SNNGNKAFQM KSRLLSPIAS NRLPPEPGLP DSYGFDYPTD
610 620 630 640 650
VGPRRLFSHP REPTLGAHGP SRHKMSPPPS SFNEPRSGGG SGGPLSHFYS
660 670 680 690 700
GSPTSYFTSG LQAGLKQSHL NKAVGSSPMG SSEGLLGLGP GPNGHSHLLK
710 720 730 740
TPLGGQKRSF SHLLPSPEPS PEGSYVGQHS QGLGGHYADS YLKRKRIF
Length:748
Mass (Da):79,382
Last modified:January 24, 2006 - v2
Checksum:iB0D775F14BD5B214
GO
Isoform 2 (identifier: Q9CW46-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     253-270: LACGQDGQLKGFAVLEYE → TCVIMASLELRDPPAFSS
     271-748: Missing.

Show »
Length:270
Mass (Da):30,392
Checksum:i99A147002C2EDA9F
GO

Sequence cautioni

The sequence BAB23670.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11M → K in BAB23670 (PubMed:11724819).Curated
Sequence conflicti397 – 3971Missing in BAD90266 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei253 – 27018LACGQ…VLEYE → TCVIMASLELRDPPAFSS in isoform 2. 1 PublicationVSP_017037Add
BLAST
Alternative sequencei271 – 748478Missing in isoform 2. 1 PublicationVSP_017038Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY275472 mRNA. Translation: AAP33691.1.
AK004919 mRNA. Translation: BAB23670.1. Different initiation.
AK039928 mRNA. Translation: BAC30481.1.
AK084478 mRNA. Translation: BAC39194.1.
AK220434 mRNA. Translation: BAD90266.1.
BC043698 mRNA. Translation: AAH43698.1.
BC108392 mRNA. Translation: AAI08393.1.
CCDSiCCDS40550.1. [Q9CW46-1]
RefSeqiNP_082187.1. NM_027911.3. [Q9CW46-1]
UniGeneiMm.196137.

Genome annotation databases

EnsembliENSMUST00000115487; ENSMUSP00000111150; ENSMUSG00000010205. [Q9CW46-1]
GeneIDi71766.
KEGGimmu:71766.
UCSCiuc009okd.2. mouse. [Q9CW46-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY275472 mRNA. Translation: AAP33691.1.
AK004919 mRNA. Translation: BAB23670.1. Different initiation.
AK039928 mRNA. Translation: BAC30481.1.
AK084478 mRNA. Translation: BAC39194.1.
AK220434 mRNA. Translation: BAD90266.1.
BC043698 mRNA. Translation: AAH43698.1.
BC108392 mRNA. Translation: AAI08393.1.
CCDSiCCDS40550.1. [Q9CW46-1]
RefSeqiNP_082187.1. NM_027911.3. [Q9CW46-1]
UniGeneiMm.196137.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WI6NMR-A61-135[»]
3ZZYX-ray1.40C/D496-507[»]
3ZZZX-ray1.55C/D680-692[»]
ProteinModelPortaliQ9CW46.
SMRiQ9CW46. Positions 39-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214910. 7 interactions.
DIPiDIP-44609N.
IntActiQ9CW46. 4 interactions.
MINTiMINT-1707126.
STRINGi10090.ENSMUSP00000111150.

PTM databases

iPTMnetiQ9CW46.
PhosphoSiteiQ9CW46.

Proteomic databases

EPDiQ9CW46.
MaxQBiQ9CW46.
PaxDbiQ9CW46.
PRIDEiQ9CW46.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000115487; ENSMUSP00000111150; ENSMUSG00000010205. [Q9CW46-1]
GeneIDi71766.
KEGGimmu:71766.
UCSCiuc009okd.2. mouse. [Q9CW46-1]

Organism-specific databases

CTDi125950.
MGIiMGI:1919016. Raver1.
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IJBP. Eukaryota.
ENOG410ZP6I. LUCA.
GeneTreeiENSGT00390000006046.
HOGENOMiHOG000253941.
HOVERGENiHBG059515.
InParanoidiQ9CW46.
OMAiHGPSRHK.
OrthoDBiEOG741Z3J.
PhylomeDBiQ9CW46.
TreeFamiTF331660.

Miscellaneous databases

ChiTaRSiRaver1. mouse.
EvolutionaryTraceiQ9CW46.
NextBioi334445.
PROiQ9CW46.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CW46.
CleanExiMM_RAVER1.
GenevisibleiQ9CW46. MM.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins."
    Huettelmaier S., Illenberger S., Grosheva I., Ruediger M., Singer R.H., Jockusch B.M.
    J. Cell Biol. 155:775-786(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INTERACTION WITH PTBP1; VCL AND ACTN1, IDENTIFICATION IN A COMPLEX WITH VCL AND ACTN1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Embryo.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Fetal heart, Liver and Thymus.
  3. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs Identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 261-748 (ISOFORM 1).
    Tissue: Fetal brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 519-748 (ISOFORM 1).
    Strain: C57BL/6J and FVB/N.
    Tissue: Embryo and Mammary gland.
  5. "The PTB interacting protein raver1 regulates alpha-tropomyosin alternative splicing."
    Gromak N., Rideau A., Southby J., Scadden A.D.J., Gooding C., Huettelmaier S., Singer R.H., Smith C.W.J.
    EMBO J. 22:6356-6364(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: INTERACTION WITH RAVER2, TISSUE SPECIFICITY.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-576 AND SER-626, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14 AND THR-469, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-469; SER-576; SER-626; SER-630; SER-716 AND SER-720, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  10. "Solution structure of the RNA binding domain from mouse hypothetical protein BAB23670."
    RIKEN structural genomics initiative (RSGI)
    Submitted (JUN-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 61-135.

Entry informationi

Entry nameiRAVR1_MOUSE
AccessioniPrimary (citable) accession number: Q9CW46
Secondary accession number(s): Q5DTT6
, Q811K0, Q8C3Z1, Q8CA14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: January 24, 2006
Last modified: May 11, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.