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Protein

Regulation of nuclear pre-mRNA domain-containing protein 1B

Gene

Rprd1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-6807505 RNA polymerase II transcribes snRNA genes

Names & Taxonomyi

Protein namesi
Recommended name:
Regulation of nuclear pre-mRNA domain-containing protein 1B
Alternative name(s):
Cell cycle-related and expression-elevated protein in tumor
Gene namesi
Name:Rprd1b
Synonyms:Crept
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1917720 Rprd1b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000794422 – 326Regulation of nuclear pre-mRNA domain-containing protein 1BAdd BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei132PhosphoserineBy similarity1
Modified residuei134PhosphoserineCombined sources1
Modified residuei161PhosphotyrosineBy similarity1
Modified residuei166PhosphoserineCombined sources1
Modified residuei299PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9CSU0
PaxDbiQ9CSU0
PeptideAtlasiQ9CSU0
PRIDEiQ9CSU0

PTM databases

iPTMnetiQ9CSU0
PhosphoSitePlusiQ9CSU0

Expressioni

Tissue specificityi

Widely expressed in the adult with highest levels in liver, colon, prostate and uterus and lowest levels in heart and kidney. Not detected in rectum.1 Publication

Developmental stagei

Highly expressed during early embryonic development (at protein level). Low levels detected in the adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000027651
ExpressionAtlasiQ9CSU0 baseline and differential
GenevisibleiQ9CSU0 MM

Interactioni

Subunit structurei

Associates with the RNA polymerase II complex.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi214076, 34 interactors
IntActiQ9CSU0, 35 interactors
MINTiQ9CSU0
STRINGi10090.ENSMUSP00000099412

Structurei

3D structure databases

ProteinModelPortaliQ9CSU0
SMRiQ9CSU0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 133CIDPROSITE-ProRule annotationAdd BLAST132

Sequence similaritiesi

Belongs to the UPF0400 (RTT103) family.Curated

Phylogenomic databases

eggNOGiKOG2669 Eukaryota
ENOG410XRAP LUCA
GeneTreeiENSGT00400000022016
HOVERGENiHBG051177
InParanoidiQ9CSU0
KOiK15559
OMAiKTWQREL
OrthoDBiEOG091G0F8X
PhylomeDBiQ9CSU0
TreeFamiTF320926

Family and domain databases

Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR006569 CID_dom
IPR032337 CREPT
IPR008942 ENTH_VHS
IPR006903 RNA_pol_II-bd
PfamiView protein in Pfam
PF16566 CREPT, 1 hit
PF04818 CTD_bind, 1 hit
SMARTiView protein in SMART
SM00582 RPR, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS51391 CID, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CSU0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSFSESALE KKLSELSNSQ QSVQTLSLWL IHHRKHAGPI VSVWHRELRK
60 70 80 90 100
AKSNRKLTFL YLANDVIQNS KRKGPEFTRE FESVLVDAFS HVAREADEGC
110 120 130 140 150
KKPLERLLNI WQERSVYGGE FIQQLKLSME DSKSPPPKAA EEKKSLKRTF
160 170 180 190 200
QQIQEEEDDD YPGSYSPQDP SAGPLLTEEL IKALQDLENA ASGDATVRQK
210 220 230 240 250
IASLPQEVQD VSLLEKITDK EAAERLSKTV DEACLLLAEY NGRLAAELED
260 270 280 290 300
RRQLARMLVE YTQNQKEVLS EKEKKLEEYK QKLARVTQVR KELKSHIQSL
310 320
PDLSLLPNVT GGLAPLPSAG DLFSTD
Length:326
Mass (Da):36,884
Last modified:October 10, 2002 - v2
Checksum:i904B9792FBBDFA71
GO
Isoform 2 (identifier: Q9CSU0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     278-294: EYKQKLARVTQVRKELK → RLTQQLQPCSDPQNVSF
     295-326: Missing.

Note: No experimental confirmation available.
Show »
Length:294
Mass (Da):33,512
Checksum:i51BDAC942555DCE2
GO
Isoform 3 (identifier: Q9CSU0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     220-224: KEAAE → AETEA
     225-326: Missing.

Note: No experimental confirmation available.
Show »
Length:224
Mass (Da):25,336
Checksum:i654C9B2E23620CC1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17S → G in BAE30966 (PubMed:15489334).Curated1
Sequence conflicti17S → G in BAE31047 (PubMed:15489334).Curated1
Sequence conflicti139Missing in BAB27954 (PubMed:16141072).Curated1
Sequence conflicti139Missing in BAE28092 (PubMed:16141072).Curated1
Sequence conflicti139Missing in BAE28237 (PubMed:16141072).Curated1
Sequence conflicti139Missing in CAM23183 (PubMed:19468303).Curated1
Sequence conflicti139Missing in CAM23185 (PubMed:19468303).Curated1
Sequence conflicti139Missing in AAH42447 (PubMed:15489334).Curated1
Sequence conflicti139Missing in AAH54350 (PubMed:15489334).Curated1
Sequence conflicti206 – 222QEVQD…TDKEA → SGSARRVAARENYRCRN in BAE24207 (PubMed:16141072).CuratedAdd BLAST17
Sequence conflicti223 – 326Missing in BAE24207 (PubMed:16141072).CuratedAdd BLAST104
Sequence conflicti270S → P in BAB27954 (PubMed:16141072).Curated1
Sequence conflicti317P → S in BAE28092 (PubMed:16141072).Curated1
Sequence conflicti317P → S in BAE28237 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_035576220 – 224KEAAE → AETEA in isoform 3. Curated5
Alternative sequenceiVSP_035577225 – 326Missing in isoform 3. CuratedAdd BLAST102
Alternative sequenceiVSP_018618278 – 294EYKQK…RKELK → RLTQQLQPCSDPQNVSF in isoform 2. 1 PublicationAdd BLAST17
Alternative sequenceiVSP_018619295 – 326Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ372939 mRNA Translation: ABD34792.1
AK011979 mRNA Translation: BAB27954.3
AK139995 mRNA Translation: BAE24207.1
AK147718 mRNA Translation: BAE28092.1
AK147930 mRNA Translation: BAE28237.1
AK152125 mRNA Translation: BAE30966.1
AK152220 mRNA Translation: BAE31047.1
AL669824 Genomic DNA Translation: CAM23181.1
AL669824 Genomic DNA Translation: CAM23182.1
AL669824 Genomic DNA Translation: CAM23183.1
AL669824 Genomic DNA Translation: CAM23184.1
AL669824 Genomic DNA Translation: CAM23185.1
BC028819 mRNA Translation: AAH28819.3
BC042447 mRNA Translation: AAH42447.1
BC054350 mRNA Translation: AAH54350.1
CCDSiCCDS71176.1 [Q9CSU0-2]
CCDS71177.1 [Q9CSU0-1]
RefSeqiNP_001278063.1, NM_001291134.1 [Q9CSU0-1]
NP_001278064.1, NM_001291135.1 [Q9CSU0-2]
NP_001278065.1, NM_001291136.1
NP_081710.1, NM_027434.3
UniGeneiMm.290810

Genome annotation databases

EnsembliENSMUST00000029180; ENSMUSP00000029180; ENSMUSG00000027651 [Q9CSU0-1]
ENSMUST00000109518; ENSMUSP00000105144; ENSMUSG00000027651 [Q9CSU0-2]
ENSMUST00000152452; ENSMUSP00000118434; ENSMUSG00000027651 [Q9CSU0-3]
GeneIDi70470
KEGGimmu:70470
UCSCiuc008npl.2 mouse [Q9CSU0-1]
uc008npn.2 mouse [Q9CSU0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRPR1B_MOUSE
AccessioniPrimary (citable) accession number: Q9CSU0
Secondary accession number(s): A2ACF2
, A2ACF4, A2ACF5, A2ACF6, Q1WDE6, Q3U8H1, Q3UGH1, Q3USX3, Q6GTJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: October 10, 2002
Last modified: March 28, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health