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Protein

Protein ecdysoneless homolog

Gene

Ecd

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of p53/TP53 stability and function. Inhibits MDM2-mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP. May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (By similarity). Involved in regulation of cell cycle progression (PubMed:26711270). Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex. May play a role in regulation of pre-mRNA splicing (By similarity).By similarity

GO - Biological processi

  • cell proliferation Source: MGI
  • mRNA processing Source: UniProtKB-KW
  • regulation of G1/S transition of mitotic cell cycle Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • RNA splicing Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

mRNA processing, mRNA splicing, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ecdysoneless homologBy similarity
Gene namesi
Name:Ecd
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1917851. Ecd.

Subcellular locationi

  • Cytoplasm
  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 641641Protein ecdysoneless homologPRO_0000220845Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei503 – 5031PhosphoserineBy similarity
Modified residuei505 – 5051PhosphoserineBy similarity
Modified residuei519 – 5191PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated predominantly by CK2 on two serine-containing clusters; involved in cell cycle regulation activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9CS74.
MaxQBiQ9CS74.
PaxDbiQ9CS74.
PRIDEiQ9CS74.

PTM databases

iPTMnetiQ9CS74.
PhosphoSiteiQ9CS74.

Expressioni

Gene expression databases

BgeeiQ9CS74.
CleanExiMM_ECD.
GenevisibleiQ9CS74. MM.

Interactioni

Subunit structurei

Interacts with TP53, MDM2, TXNIP. Interacts (phosphorylated) with PIH1D1. Interacts with RUVBL1 mediating the PIH1D1-independent association with the R2TP complex. Interacts with RB1, RBL1 and RBL2; ECD competes with E2F1 for binding to hypophospshorylated RB1. Interacts with EP300.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Itgb1bp2Q9R0002EBI-7922565,EBI-7922331

Protein-protein interaction databases

BioGridi214157. 9 interactions.
IntActiQ9CS74. 11 interactions.
STRINGi10090.ENSMUSP00000022344.

Structurei

3D structure databases

ProteinModelPortaliQ9CS74.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni439 – 641203Transcription activationBy similarityAdd
BLAST
Regioni481 – 49717Involved in nuclear exportBy similarityAdd
BLAST
Regioni502 – 53130Acidic region required for transactivation activityBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the ECD family.Curated

Phylogenomic databases

eggNOGiKOG2406. Eukaryota.
ENOG410XR07. LUCA.
GeneTreeiENSGT00390000015361.
HOGENOMiHOG000029899.
HOVERGENiHBG023145.
InParanoidiQ9CS74.
OMAiIVYIVKQ.
OrthoDBiEOG7M3J13.
PhylomeDBiQ9CS74.
TreeFamiTF324229.

Family and domain databases

InterProiIPR010770. Ecd.
[Graphical view]
PANTHERiPTHR13060. PTHR13060. 1 hit.
PfamiPF07093. SGT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CS74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGSGKLAMV EDAVEYHLFL IPDKARGTEE HREILQKYIE RIMTQFAPIL
60 70 80 90 100
VPYIWQNQPF NLKYKPAKGG VPAHMYGMTK FGDNIEDEWF IVYVIKQITK
110 120 130 140 150
EFPELVARVE DNDGEFLLIE AADFLPKWLD PDNSANRVFF HHGELCIIPV
160 170 180 190 200
PRKSERIPWL PMTPPTIQQA LSIISAHPEA VLASESIQAA VDRRVSGYPE
210 220 230 240 250
RVEASLHRAH CFLPAGIVAV LKQQPRLLSA AVQAFYLRDP IDLRACRVFK
260 270 280 290 300
TFLPETRIMA SVTFTKCLYA QLVQQKFVPD RRSGYGLPPP SHPQYRAYEL
310 320 330 340 350
GMKLAHGFEI LCSKCSPHFS DSRKSLVTAS PLWASFLESL KRNDYFKGLM
360 370 380 390 400
DGSAQYQERL EMAKNYFQLS IHRPESSLAM SPGEEILTVL QTQPFDVAEL
410 420 430 440 450
KTEEADLPPE DDDQWLDLSP DQLDQLLQDA AGRKESQPGP QKEELQNYDV
460 470 480 490 500
AQVSDSMKAF ISKVSSHKGA ELPRDPSEAP ITFDADSFLN YFDKILGAKP
510 520 530 540 550
QESDSEDDPG EEDVEGVDSD DDVGFEAQES ESLKGALGSL KSYMARMDQE
560 570 580 590 600
LAHTSMGRSF TTRERLNKDP PSHTANDNSD EEDSGAGDCA VEAVDVDLNL
610 620 630 640
ISNILESYSS QAGLAGPASN LLHSMGVRLP DNADHNPQVS Q
Length:641
Mass (Da):71,710
Last modified:November 16, 2001 - v2
Checksum:i679C6E2EE6404357
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028664 mRNA. Translation: BAC26054.1.
BC013470 mRNA. Translation: AAH13470.1.
CCDSiCCDS26842.1.
RefSeqiNP_081751.1. NM_027475.3.
UniGeneiMm.35105.

Genome annotation databases

EnsembliENSMUST00000022344; ENSMUSP00000022344; ENSMUSG00000021810.
GeneIDi70601.
KEGGimmu:70601.
UCSCiuc011zgo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK028664 mRNA. Translation: BAC26054.1.
BC013470 mRNA. Translation: AAH13470.1.
CCDSiCCDS26842.1.
RefSeqiNP_081751.1. NM_027475.3.
UniGeneiMm.35105.

3D structure databases

ProteinModelPortaliQ9CS74.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214157. 9 interactions.
IntActiQ9CS74. 11 interactions.
STRINGi10090.ENSMUSP00000022344.

PTM databases

iPTMnetiQ9CS74.
PhosphoSiteiQ9CS74.

Proteomic databases

EPDiQ9CS74.
MaxQBiQ9CS74.
PaxDbiQ9CS74.
PRIDEiQ9CS74.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022344; ENSMUSP00000022344; ENSMUSG00000021810.
GeneIDi70601.
KEGGimmu:70601.
UCSCiuc011zgo.1. mouse.

Organism-specific databases

CTDi11319.
MGIiMGI:1917851. Ecd.

Phylogenomic databases

eggNOGiKOG2406. Eukaryota.
ENOG410XR07. LUCA.
GeneTreeiENSGT00390000015361.
HOGENOMiHOG000029899.
HOVERGENiHBG023145.
InParanoidiQ9CS74.
OMAiIVYIVKQ.
OrthoDBiEOG7M3J13.
PhylomeDBiQ9CS74.
TreeFamiTF324229.

Miscellaneous databases

ChiTaRSiEcd. mouse.
PROiQ9CS74.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CS74.
CleanExiMM_ECD.
GenevisibleiQ9CS74. MM.

Family and domain databases

InterProiIPR010770. Ecd.
[Graphical view]
PANTHERiPTHR13060. PTHR13060. 1 hit.
PfamiPF07093. SGT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  3. Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.
  5. "A novel interaction of ecdysoneless (ECD) protein with R2TP complex component RUVBL1 is required for the functional role of ECD in cell cycle progression."
    Mir R.A., Bele A., Mirza S., Srivastava S., Olou A.A., Ammons S.A., Kim J.H., Gurumurthy C.B., Qiu F., Band H., Band V.
    Mol. Cell. Biol. 36:886-899(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiECD_MOUSE
AccessioniPrimary (citable) accession number: Q9CS74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 16, 2001
Last modified: June 8, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.