Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vitamin K epoxide reductase complex subunit 1

Gene

Vkorc1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K. Vitamin K is required for the gamma-carboxylation of various proteins, including clotting factors, and is required for normal blood coagulation, but also for normal bone development.2 Publications

Catalytic activityi

Phylloquinone + oxidized dithiothreitol + H2O = 2,3-epoxy-2-methyl-3-phytyl-2,3-dihydro-1,4-naphthoquinone + 1,4-dithiothreitol.

Enzyme regulationi

Inhibited by warfarin (coumadin).1 Publication

GO - Molecular functioni

  • quinone binding Source: UniProtKB-KW
  • vitamin-K-epoxide reductase (warfarin-sensitive) activity Source: MGI

GO - Biological processi

  • blood coagulation Source: UniProtKB
  • bone development Source: UniProtKB
  • drug metabolic process Source: MGI
  • peptidyl-glutamic acid carboxylation Source: UniProtKB
  • positive regulation of coagulation Source: MGI
  • vitamin K biosynthetic process Source: MGI
  • vitamin K metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

ReactomeiR-MMU-6806664. Metabolism of vitamin K.

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin K epoxide reductase complex subunit 1 (EC:1.17.4.4)
Alternative name(s):
Vitamin K1 2,3-epoxide reductase subunit 1
Gene namesi
Name:Vkorc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:106442. Vkorc1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88LumenalSequence analysis
Transmembranei9 – 2921HelicalSequence analysisAdd
BLAST
Topological domaini30 – 7849CytoplasmicSequence analysisAdd
BLAST
Transmembranei79 – 9719HelicalSequence analysisAdd
BLAST
Transmembranei101 – 12323HelicalSequence analysisAdd
BLAST
Topological domaini124 – 1263LumenalSequence analysis
Transmembranei127 – 14923HelicalSequence analysisAdd
BLAST
Topological domaini150 – 16112CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are born at the expected Mendelian rate and appear normal, but die between one and twenty days after birth, due to severe bleeeding. In about 75% of the cases, subdural bleeding is observed, in addition to intracerebral and intramuscular bleeding. Daily oral administration of vitamin K to the mutant mice leads to normal survival, but the mice die within seven days after the cessation of vitamin K administration. Besides, both homozygous and heterozygous mutant mice display defects in bone development with reduced length of the calcified part of the long bones in front and hind limbs.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Vitamin K epoxide reductase complex subunit 1PRO_0000191669Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi132 ↔ 135Redox-activeBy similarity

Keywords - PTMi

Disulfide bond, Quinone

Proteomic databases

EPDiQ9CRC0.
MaxQBiQ9CRC0.
PaxDbiQ9CRC0.
PeptideAtlasiQ9CRC0.
PRIDEiQ9CRC0.

PTM databases

PhosphoSiteiQ9CRC0.

Expressioni

Tissue specificityi

Detected in liver.1 Publication

Gene expression databases

BgeeiQ9CRC0.
CleanExiMM_VKORC1.
ExpressionAtlasiQ9CRC0. baseline and differential.
GenevisibleiQ9CRC0. MM.

Interactioni

Protein-protein interaction databases

BioGridi205697. 1 interaction.
STRINGi10090.ENSMUSP00000033074.

Structurei

3D structure databases

ProteinModelPortaliQ9CRC0.
SMRiQ9CRC0. Positions 4-153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The number of transmembrane domains and the membrane topology are controversial; supporting evidence is available both for models with three transmembrane domains and four transmembrane domains.By similarity

Sequence similaritiesi

Belongs to the VKOR family.Curated

Keywords - Domaini

Redox-active center, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410J0HT. Eukaryota.
ENOG4111UX7. LUCA.
GeneTreeiENSGT00390000002103.
HOGENOMiHOG000230752.
HOVERGENiHBG076672.
InParanoidiQ9CRC0.
KOiK05357.
OMAiRARWASI.
OrthoDBiEOG7TQV2D.
PhylomeDBiQ9CRC0.
TreeFamiTF328467.

Family and domain databases

InterProiIPR012932. VKOR.
[Graphical view]
PfamiPF07884. VKOR. 1 hit.
[Graphical view]
SMARTiSM00756. VKc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CRC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTTWRSPGL VRLALCLAGL ALSLYALHVK AARARDENYR ALCDVGTAIS
60 70 80 90 100
CSRVFSSRWG RGFGLVEHML GADSVLNQSN SIFGCLFYTL QLLLGCLRGR
110 120 130 140 150
WASILLVLSS LVSVAGSVYL AWILFFVLYD FCIVCITTYA INVGLMLLSF
160
QKVPEHKTKK H
Length:161
Mass (Da):17,768
Last modified:June 1, 2001 - v1
Checksum:i044BEED047A57FD9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti128 – 1281L → S Identified in warfarin-resistant animals. 1 Publication
Natural varianti139 – 1391Y → C Identified in warfarin-resistant animals. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013996 mRNA. Translation: BAB29106.1.
AK002742 mRNA. Translation: BAB22320.1.
AK003237 mRNA. Translation: BAB22661.1.
AK008509 mRNA. Translation: BAB25708.1.
AK008578 mRNA. Translation: BAB25757.1.
BC031732 mRNA. Translation: AAH31732.1.
CCDSiCCDS21883.1.
RefSeqiNP_848715.1. NM_178600.2.
UniGeneiMm.29703.

Genome annotation databases

EnsembliENSMUST00000033074; ENSMUSP00000033074; ENSMUSG00000096145.
GeneIDi27973.
KEGGimmu:27973.
UCSCiuc009jxe.1. mouse.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013996 mRNA. Translation: BAB29106.1.
AK002742 mRNA. Translation: BAB22320.1.
AK003237 mRNA. Translation: BAB22661.1.
AK008509 mRNA. Translation: BAB25708.1.
AK008578 mRNA. Translation: BAB25757.1.
BC031732 mRNA. Translation: AAH31732.1.
CCDSiCCDS21883.1.
RefSeqiNP_848715.1. NM_178600.2.
UniGeneiMm.29703.

3D structure databases

ProteinModelPortaliQ9CRC0.
SMRiQ9CRC0. Positions 4-153.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205697. 1 interaction.
STRINGi10090.ENSMUSP00000033074.

PTM databases

PhosphoSiteiQ9CRC0.

Proteomic databases

EPDiQ9CRC0.
MaxQBiQ9CRC0.
PaxDbiQ9CRC0.
PeptideAtlasiQ9CRC0.
PRIDEiQ9CRC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033074; ENSMUSP00000033074; ENSMUSG00000096145.
GeneIDi27973.
KEGGimmu:27973.
UCSCiuc009jxe.1. mouse.

Organism-specific databases

CTDi79001.
MGIiMGI:106442. Vkorc1.

Phylogenomic databases

eggNOGiENOG410J0HT. Eukaryota.
ENOG4111UX7. LUCA.
GeneTreeiENSGT00390000002103.
HOGENOMiHOG000230752.
HOVERGENiHBG076672.
InParanoidiQ9CRC0.
KOiK05357.
OMAiRARWASI.
OrthoDBiEOG7TQV2D.
PhylomeDBiQ9CRC0.
TreeFamiTF328467.

Enzyme and pathway databases

ReactomeiR-MMU-6806664. Metabolism of vitamin K.

Miscellaneous databases

ChiTaRSiVkorc1. mouse.
PROiQ9CRC0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CRC0.
CleanExiMM_VKORC1.
ExpressionAtlasiQ9CRC0. baseline and differential.
GenevisibleiQ9CRC0. MM.

Family and domain databases

InterProiIPR012932. VKOR.
[Graphical view]
PfamiPF07884. VKOR. 1 hit.
[Graphical view]
SMARTiSM00756. VKc. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head, Kidney and Small intestine.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  3. "VKORC1 deficiency in mice causes early postnatal lethality due to severe bleeding."
    Spohn G., Kleinridders A., Wunderlich F.T., Watzka M., Zaucke F., Blumbach K., Geisen C., Seifried E., Muller C., Paulsson M., Bruning J.C., Oldenburg J.
    Thromb. Haemost. 101:1044-1050(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION, TISSUE SPECIFICITY.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Kidney, Liver, Lung, Pancreas and Testis.
  5. Cited for: VARIANTS SER-128 AND CYS-139, ENZYME REGULATION, FUNCTION.

Entry informationi

Entry nameiVKOR1_MOUSE
AccessioniPrimary (citable) accession number: Q9CRC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: June 1, 2001
Last modified: July 6, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The location of two cysteine active-site residues within a proposed transmembrane is consistent both with the known hydrophobic environment of the thiol redox site of the enzyme and with the lipophilicity of vitamin K and warfarin (coumadin).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.