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Protein

Golgi phosphoprotein 3

Gene

Golph3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811PtdIns4PBy similarity
Binding sitei90 – 901PtdIns4PBy similarity
Binding sitei171 – 1711PtdIns4PBy similarity
Binding sitei174 – 1741PtdIns4PBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi phosphoprotein 3
Alternative name(s):
Coat protein GPP34
Gene namesi
Name:Golph3
Synonyms:Gpp34
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1913879. Golph3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Golgi apparatus, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 298298Golgi phosphoprotein 3PRO_0000123820Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351PhosphoserineCombined sources
Disulfide bondi84 ↔ 108By similarity

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ9CRA5.
MaxQBiQ9CRA5.
PaxDbiQ9CRA5.
PeptideAtlasiQ9CRA5.
PRIDEiQ9CRA5.

PTM databases

iPTMnetiQ9CRA5.
PhosphoSiteiQ9CRA5.

Expressioni

Tissue specificityi

Expressed in all tissues tested including brain, heart, kidney, liver, lung, salivary gland, skeletal muscle, small intestine, spleen, stomach, skin and testis (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000022200.
CleanExiMM_GOLPH3.
GenevisibleiQ9CRA5. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with the coatomer complex. Interacts with MYO18A; the interaction is direct and may link Golgi membranes to the actin cytoskeleton. Interacts with GCNT1; may control its retention in the Golgi. Interacts with VPS35 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211608. 1 interaction.
IntActiQ9CRA5. 1 interaction.
MINTiMINT-1857422.
STRINGi10090.ENSMUSP00000057375.

Structurei

3D structure databases

ProteinModelPortaliQ9CRA5.
SMRiQ9CRA5. Positions 59-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni190 – 20112Beta-hairpin required for oligomerizationBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi30 – 367Poly-Gly
Compositional biasi48 – 514Poly-Asp

Sequence similaritiesi

Belongs to the GOLPH3/VPS74 family.Curated

Phylogenomic databases

eggNOGiKOG3983. Eukaryota.
ENOG410XRT6. LUCA.
GeneTreeiENSGT00390000007153.
HOGENOMiHOG000209848.
HOVERGENiHBG059607.
InParanoidiQ9CRA5.
KOiK15620.
OMAiESMKANT.
OrthoDBiEOG091G0M99.
PhylomeDBiQ9CRA5.
TreeFamiTF314360.

Family and domain databases

InterProiIPR008628. GPP34.
[Graphical view]
PANTHERiPTHR12704. PTHR12704. 1 hit.
PfamiPF05719. GPP34. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CRA5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSLTQRSSG LVQRRTEASR NAADKERAAG GGGGSGEDEA QSRRDEQDDD
60 70 80 90 100
DKGDSKETRL TLMEEVLLLG LKDREGYTSF WNDCISSGLR GCMLIELALR
110 120 130 140 150
GRLQLEACGM RRKSLLTRKV ICKSDAPTGD VLLDEALKHV KETQPPETVQ
160 170 180 190 200
NWIELLSGET WNPLKLHYQL RNVRERLAKN LVEKGVLTTE KQNFLLFDMT
210 220 230 240 250
THPLTNNNIK QRLIKKVQEA VLDKWVNDPH RMDKRLLALI YLAHASDVLE
260 270 280 290
NAFAPLLDEQ YDLATKRVRQ LLDLDPEVEC LKANTNEVLW AVVAAFTK
Length:298
Mass (Da):33,752
Last modified:June 1, 2001 - v1
Checksum:i09AC126ED98F6370
GO
Isoform 2 (identifier: Q9CRA5-2) [UniParc]FASTAAdd to basket
Also known as: GMx33alphaV, GPP34V

The sequence of this isoform differs from the canonical sequence as follows:
     76-81: GYTSFW → VRRGIH
     82-298: Missing.

Show »
Length:81
Mass (Da):8,901
Checksum:i30585C933CE14C71
GO

Sequence cautioni

The sequence BAB29486 differs from that shown. Reason: Frameshift at position 160. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei76 – 816GYTSFW → VRRGIH in isoform 2. 1 PublicationVSP_037833
Alternative sequencei82 – 298217Missing in isoform 2. 1 PublicationVSP_037834Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB271918 mRNA. Translation: BAF64720.1.
AK005996 mRNA. Translation: BAB24357.1.
AK014644 mRNA. Translation: BAB29486.1. Frameshift.
AK015543 mRNA. Translation: BAB29889.1.
BC003961 mRNA. Translation: AAH03961.1.
BC051035 mRNA. Translation: AAH51035.1.
CCDSiCCDS27389.1. [Q9CRA5-1]
RefSeqiNP_079949.1. NM_025673.2. [Q9CRA5-1]
XP_011243680.1. XM_011245378.1.
UniGeneiMm.157655.

Genome annotation databases

EnsembliENSMUST00000059680; ENSMUSP00000057375; ENSMUSG00000022200. [Q9CRA5-1]
GeneIDi66629.
KEGGimmu:66629.
UCSCiuc007vhn.1. mouse. [Q9CRA5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB271918 mRNA. Translation: BAF64720.1.
AK005996 mRNA. Translation: BAB24357.1.
AK014644 mRNA. Translation: BAB29486.1. Frameshift.
AK015543 mRNA. Translation: BAB29889.1.
BC003961 mRNA. Translation: AAH03961.1.
BC051035 mRNA. Translation: AAH51035.1.
CCDSiCCDS27389.1. [Q9CRA5-1]
RefSeqiNP_079949.1. NM_025673.2. [Q9CRA5-1]
XP_011243680.1. XM_011245378.1.
UniGeneiMm.157655.

3D structure databases

ProteinModelPortaliQ9CRA5.
SMRiQ9CRA5. Positions 59-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211608. 1 interaction.
IntActiQ9CRA5. 1 interaction.
MINTiMINT-1857422.
STRINGi10090.ENSMUSP00000057375.

PTM databases

iPTMnetiQ9CRA5.
PhosphoSiteiQ9CRA5.

Proteomic databases

EPDiQ9CRA5.
MaxQBiQ9CRA5.
PaxDbiQ9CRA5.
PeptideAtlasiQ9CRA5.
PRIDEiQ9CRA5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059680; ENSMUSP00000057375; ENSMUSG00000022200. [Q9CRA5-1]
GeneIDi66629.
KEGGimmu:66629.
UCSCiuc007vhn.1. mouse. [Q9CRA5-1]

Organism-specific databases

CTDi64083.
MGIiMGI:1913879. Golph3.

Phylogenomic databases

eggNOGiKOG3983. Eukaryota.
ENOG410XRT6. LUCA.
GeneTreeiENSGT00390000007153.
HOGENOMiHOG000209848.
HOVERGENiHBG059607.
InParanoidiQ9CRA5.
KOiK15620.
OMAiESMKANT.
OrthoDBiEOG091G0M99.
PhylomeDBiQ9CRA5.
TreeFamiTF314360.

Miscellaneous databases

PROiQ9CRA5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022200.
CleanExiMM_GOLPH3.
GenevisibleiQ9CRA5. MM.

Family and domain databases

InterProiIPR008628. GPP34.
[Graphical view]
PANTHERiPTHR12704. PTHR12704. 1 hit.
PfamiPF05719. GPP34. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGOLP3_MOUSE
AccessioniPrimary (citable) accession number: Q9CRA5
Secondary accession number(s): A6BLP3, Q99KY1, Q9D636
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.