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Protein

Golgi phosphoprotein 3

Gene

Golph3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei81PtdIns4PBy similarity1
Binding sitei90PtdIns4PBy similarity1
Binding sitei171PtdIns4PBy similarity1
Binding sitei174PtdIns4PBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Golgi phosphoprotein 3
Alternative name(s):
Coat protein GPP34
Gene namesi
Name:Golph3
Synonyms:Gpp34
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1913879 Golph3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Endosome, Golgi apparatus, Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001238201 – 298Golgi phosphoprotein 3Add BLAST298

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35PhosphoserineCombined sources1
Disulfide bondi84 ↔ 108By similarity

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ9CRA5
PaxDbiQ9CRA5
PeptideAtlasiQ9CRA5
PRIDEiQ9CRA5

PTM databases

iPTMnetiQ9CRA5
PhosphoSitePlusiQ9CRA5

Expressioni

Tissue specificityi

Expressed in all tissues tested including brain, heart, kidney, liver, lung, salivary gland, skeletal muscle, small intestine, spleen, stomach, skin and testis (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000022200
CleanExiMM_GOLPH3
GenevisibleiQ9CRA5 MM

Interactioni

Subunit structurei

Homodimer. Interacts with the coatomer complex. Interacts with MYO18A; the interaction is direct and may link Golgi membranes to the actin cytoskeleton. Interacts with GCNT1; may control its retention in the Golgi. Interacts with VPS35 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211608, 2 interactors
IntActiQ9CRA5, 1 interactor
STRINGi10090.ENSMUSP00000057375

Structurei

3D structure databases

ProteinModelPortaliQ9CRA5
SMRiQ9CRA5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 201Beta-hairpin required for oligomerizationBy similarityAdd BLAST12

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 36Poly-Gly7
Compositional biasi48 – 51Poly-Asp4

Sequence similaritiesi

Belongs to the GOLPH3/VPS74 family.Curated

Phylogenomic databases

eggNOGiKOG3983 Eukaryota
ENOG410XRT6 LUCA
GeneTreeiENSGT00390000007153
HOGENOMiHOG000209848
HOVERGENiHBG059607
InParanoidiQ9CRA5
KOiK15620
OMAiVMKIGFQ
OrthoDBiEOG091G0M99
PhylomeDBiQ9CRA5
TreeFamiTF314360

Family and domain databases

Gene3Di1.10.3630.10, 1 hit
InterProiView protein in InterPro
IPR008628 GPP34-like
IPR038261 GPP34-like_sf
PANTHERiPTHR12704 PTHR12704, 1 hit
PfamiView protein in Pfam
PF05719 GPP34, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CRA5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTSLTQRSSG LVQRRTEASR NAADKERAAG GGGGSGEDEA QSRRDEQDDD
60 70 80 90 100
DKGDSKETRL TLMEEVLLLG LKDREGYTSF WNDCISSGLR GCMLIELALR
110 120 130 140 150
GRLQLEACGM RRKSLLTRKV ICKSDAPTGD VLLDEALKHV KETQPPETVQ
160 170 180 190 200
NWIELLSGET WNPLKLHYQL RNVRERLAKN LVEKGVLTTE KQNFLLFDMT
210 220 230 240 250
THPLTNNNIK QRLIKKVQEA VLDKWVNDPH RMDKRLLALI YLAHASDVLE
260 270 280 290
NAFAPLLDEQ YDLATKRVRQ LLDLDPEVEC LKANTNEVLW AVVAAFTK
Length:298
Mass (Da):33,752
Last modified:June 1, 2001 - v1
Checksum:i09AC126ED98F6370
GO
Isoform 2 (identifier: Q9CRA5-2) [UniParc]FASTAAdd to basket
Also known as: GMx33alphaV, GPP34V

The sequence of this isoform differs from the canonical sequence as follows:
     76-81: GYTSFW → VRRGIH
     82-298: Missing.

Show »
Length:81
Mass (Da):8,901
Checksum:i30585C933CE14C71
GO

Sequence cautioni

The sequence BAB29486 differs from that shown. Reason: Frameshift at position 160.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03783376 – 81GYTSFW → VRRGIH in isoform 2. 1 Publication6
Alternative sequenceiVSP_03783482 – 298Missing in isoform 2. 1 PublicationAdd BLAST217

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB271918 mRNA Translation: BAF64720.1
AK005996 mRNA Translation: BAB24357.1
AK014644 mRNA Translation: BAB29486.1 Frameshift.
AK015543 mRNA Translation: BAB29889.1
BC003961 mRNA Translation: AAH03961.1
BC051035 mRNA Translation: AAH51035.1
CCDSiCCDS27389.1 [Q9CRA5-1]
RefSeqiNP_079949.1, NM_025673.2 [Q9CRA5-1]
UniGeneiMm.157655

Genome annotation databases

EnsembliENSMUST00000059680; ENSMUSP00000057375; ENSMUSG00000022200 [Q9CRA5-1]
ENSMUST00000226517; ENSMUSP00000153929; ENSMUSG00000022200 [Q9CRA5-2]
GeneIDi66629
KEGGimmu:66629
UCSCiuc007vhn.1 mouse [Q9CRA5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGOLP3_MOUSE
AccessioniPrimary (citable) accession number: Q9CRA5
Secondary accession number(s): A6BLP3, Q99KY1, Q9D636
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health