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Protein

Methylsterol monooxygenase 1

Gene

Msmo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the first step in the removal of the two C-4 methyl groups of 4,4-dimethylzymosterol.By similarity

Catalytic activityi

4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)+ + H2O.By similarity
4-beta-hydroxymethyl-4-alpha-methyl-5-alpha-cholest-7-en-3-beta-ol + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)+ + 2 H2O.By similarity
3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carbaldehyde + NAD(P)H + O2 = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + NAD(P)+ + H2O.By similarity

Cofactori

Fe cationBy similarity

Pathwayi: zymosterol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (Cyp51a1)
  2. no protein annotated in this organism
  3. Methylsterol monooxygenase 1 (Msmo1)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (Nsdhl)
  5. 3-keto-steroid reductase (Hsd17b7)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Iron, NAD

Enzyme and pathway databases

ReactomeiR-MMU-191273. Cholesterol biosynthesis.
UniPathwayiUPA00770; UER00756.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylsterol monooxygenase 1 (EC:1.14.13.72By similarity)
Alternative name(s):
C-4 methylsterol oxidase
Gene namesi
Name:Msmo1
Synonyms:Sc4mol
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1913484. Msmo1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001170341 – 293Methylsterol monooxygenase 1Add BLAST293

Proteomic databases

EPDiQ9CRA4.
MaxQBiQ9CRA4.
PaxDbiQ9CRA4.
PeptideAtlasiQ9CRA4.
PRIDEiQ9CRA4.

PTM databases

iPTMnetiQ9CRA4.
PhosphoSitePlusiQ9CRA4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031604.
CleanExiMM_SC4MOL.
ExpressionAtlasiQ9CRA4. baseline and differential.
GenevisibleiQ9CRA4. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034015.

Structurei

3D structure databases

ProteinModelPortaliQ9CRA4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi157 – 161Histidine box-15
Motifi170 – 174Histidine box-25
Motifi249 – 255Histidine box-37

Domaini

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0873. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00530000063017.
HOGENOMiHOG000162289.
HOVERGENiHBG051504.
InParanoidiQ9CRA4.
KOiK07750.
OMAiSSTFTWW.
OrthoDBiEOG091G0BM3.
PhylomeDBiQ9CRA4.
TreeFamiTF354294.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CRA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATNKSVGVF SSASLAVEYV DSLLPENPLQ EPFKNAWVYM LDNYTKFQIA
60 70 80 90 100
TWGSLIVHEA IYFLFSLPGF LFQFIPYMRK YKIQKDKPET FEGQWKCLKK
110 120 130 140 150
ILFNHFFIQL PLICGTYYFT EFFNIPYDWE RMPRWYLTLA RCLGCAVIED
160 170 180 190 200
TWHYFLHRLL HHKRIYKYIH KVHHEFQAPF GIEAEYAHPL ETLILGTGFF
210 220 230 240 250
IGIVLLCDHV ILLWAWVTIR LLETIDVHSG YDIPLNPLNL VPFYTGARHH
260 270 280 290
DFHHMNFIGN YASTFTWWDK LFGTDAQYHA YIEKSKKLGK KSD
Length:293
Mass (Da):34,772
Last modified:June 1, 2001 - v1
Checksum:i0A275DE7B5D4DB52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005090 mRNA. Translation: BAB23811.1.
AK005441 mRNA. Translation: BAB24035.1.
AK045059 mRNA. Translation: BAC32201.1.
AK146684 mRNA. Translation: BAE27358.1.
AK151135 mRNA. Translation: BAE30143.1.
AK169017 mRNA. Translation: BAE40814.1.
BC006802 mRNA. Translation: AAH06802.1.
CCDSiCCDS22328.1.
RefSeqiNP_079712.1. NM_025436.2.
UniGeneiMm.30119.

Genome annotation databases

EnsembliENSMUST00000034015; ENSMUSP00000034015; ENSMUSG00000031604.
GeneIDi66234.
KEGGimmu:66234.
UCSCiuc009lux.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005090 mRNA. Translation: BAB23811.1.
AK005441 mRNA. Translation: BAB24035.1.
AK045059 mRNA. Translation: BAC32201.1.
AK146684 mRNA. Translation: BAE27358.1.
AK151135 mRNA. Translation: BAE30143.1.
AK169017 mRNA. Translation: BAE40814.1.
BC006802 mRNA. Translation: AAH06802.1.
CCDSiCCDS22328.1.
RefSeqiNP_079712.1. NM_025436.2.
UniGeneiMm.30119.

3D structure databases

ProteinModelPortaliQ9CRA4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034015.

PTM databases

iPTMnetiQ9CRA4.
PhosphoSitePlusiQ9CRA4.

Proteomic databases

EPDiQ9CRA4.
MaxQBiQ9CRA4.
PaxDbiQ9CRA4.
PeptideAtlasiQ9CRA4.
PRIDEiQ9CRA4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034015; ENSMUSP00000034015; ENSMUSG00000031604.
GeneIDi66234.
KEGGimmu:66234.
UCSCiuc009lux.1. mouse.

Organism-specific databases

CTDi6307.
MGIiMGI:1913484. Msmo1.

Phylogenomic databases

eggNOGiKOG0873. Eukaryota.
COG3000. LUCA.
GeneTreeiENSGT00530000063017.
HOGENOMiHOG000162289.
HOVERGENiHBG051504.
InParanoidiQ9CRA4.
KOiK07750.
OMAiSSTFTWW.
OrthoDBiEOG091G0BM3.
PhylomeDBiQ9CRA4.
TreeFamiTF354294.

Enzyme and pathway databases

UniPathwayiUPA00770; UER00756.
ReactomeiR-MMU-191273. Cholesterol biosynthesis.

Miscellaneous databases

ChiTaRSiMsmo1. mouse.
PROiQ9CRA4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031604.
CleanExiMM_SC4MOL.
ExpressionAtlasiQ9CRA4. baseline and differential.
GenevisibleiQ9CRA4. MM.

Family and domain databases

InterProiIPR006694. Fatty_acid_hydroxylase.
[Graphical view]
PfamiPF04116. FA_hydroxylase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMSMO1_MOUSE
AccessioniPrimary (citable) accession number: Q9CRA4
Secondary accession number(s): Q543V8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.