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Protein

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Gene

Uqcrfs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochrome b-c1 complex subunit Rieske, mitochondrial: Component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis (PubMed:28673544). Incorporation of UQCRFS1 is the penultimate step in complex III assembly (By similarity).By similarity1 Publication
Cytochrome b-c1 complex subunit 9: Possible component of the mitochondrial ubiquinol-cytochrome c reductase complex dimer (complex III dimer), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis (PubMed:28673544). UQCRFS1 undergoes proteolytic processing once it is incorporated in the complex III dimer, including this fragment, called subunit 9, which corresponds to the transit peptide (PubMed:28673544). The proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer, but the persistence of UQCRFS1-derived fragments may prevent newly imported UQCRFS1 to be processed and assembled into complex III and is detrimental for the complex III structure and function (PubMed:28673544). It is therefore unsure whether the UQCRFS1 fragments, including this fragment, are structural subunits (PubMed:28673544).1 Publication

Miscellaneous

The Rieske protein is a high potential 2Fe-2S protein.

Caution

Several peptides are generated during UQCRFS1 insertion (PubMed:28673544). According to some authors, the identification of the transit peptide as the subunit 9, does not necessary imply that it must be considered as a structural subunit of the complex III dimer as additional fragments from UQCRFS1 are also present (PubMed:28673544).1 Publication

Catalytic activityi

Quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+.

Cofactori

[2Fe-2S] clusterPROSITE-ProRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit. Fe-S cluster delivery to the Rieske protein is mediated by components of the iron sulfur (Fe-S) cluster assembly machinery that reside in the mitochondrial matrix (including HSC20 and LYRM7) (By similarity).PROSITE-ProRule annotationBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi217Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi219Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi236Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi239Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, Iron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:1.10.2.2)
Alternative name(s):
Complex III subunit 5
Cytochrome b-c1 complex subunit 5
Rieske iron-sulfur protein
Short name:
RISP
Rieske protein UQCRFS1Curated
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Cleaved into the following chain:
Cytochrome b-c1 complex subunit 9By similarity
Short name:
Su9By similarity
Short name:
Subunit 9By similarity
Alternative name(s):
8 kDa subunit 9By similarity
Complex III subunit IX
Cytochrome b-c1 complex subunit 11
Ubiquinol-cytochrome c reductase 8 kDa proteinBy similarity
Gene namesi
Name:Uqcrfs1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1913944 Uqcrfs1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei103 – 140HelicalBy similarityAdd BLAST38

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 78MitochondrionBy similarityAdd BLAST78
ChainiPRO_00003072441 – 78Cytochrome b-c1 complex subunit 91 PublicationAdd BLAST78
ChainiPRO_000003066779 – 274Cytochrome b-c1 complex subunit Rieske, mitochondrialAdd BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi222 ↔ 238PROSITE-ProRule annotation

Post-translational modificationi

Proteolytic processing is necessary for the correct insertion of UQCRFS1 in the complex III dimer (PubMed:28673544). Several fragments are generated during UQCRFS1 insertion, most probably due to the endogenous matrix-processing peptidase (MPP) activity (PubMed:28673544). The action of the protease is also necessary for the clearance of the UQCRFS1 fragments (PubMed:28673544).1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ9CR68
MaxQBiQ9CR68
PaxDbiQ9CR68
PeptideAtlasiQ9CR68
PRIDEiQ9CR68

PTM databases

iPTMnetiQ9CR68
PhosphoSitePlusiQ9CR68
SwissPalmiQ9CR68

Expressioni

Gene expression databases

BgeeiENSMUSG00000038462
GenevisibleiQ9CR68 MM

Interactioni

Subunit structurei

Binds to the mitochondrial respiratory complex III dimer. The monomeric module of the mitochondrial respiratory complex III contains 11 subunits: 3 respiratory subunits involved in its catalytic activity (cytochrome b, cytochrome c1 and Rieske protein UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1) (By similarity). Incorporation of the Rieske protein UQCRFS1 is the penultimate step in complex III assembly (By similarity). Interacts with TTC19, which is involved in the clearance of UQCRFS1 fragments (PubMed:28673544).By similarity1 Publication

Protein-protein interaction databases

BioGridi211651, 2 interactors
ComplexPortaliCPX-563 Mitochondrial respiratory chain complex III
CORUMiQ9CR68
IntActiQ9CR68, 4 interactors
MINTiQ9CR68
STRINGi10090.ENSMUSP00000045284

Structurei

3D structure databases

ProteinModelPortaliQ9CR68
SMRiQ9CR68
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 272RieskePROSITE-ProRule annotationAdd BLAST86

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1671 Eukaryota
COG0723 LUCA
GeneTreeiENSGT00390000001014
HOGENOMiHOG000255193
HOVERGENiHBG001040
InParanoidiQ9CR68
KOiK00411
OMAiERKAFSY
OrthoDBiEOG091G0MOP
PhylomeDBiQ9CR68
TreeFamiTF105037

Family and domain databases

Gene3Di1.20.5.270, 1 hit
2.102.10.10, 1 hit
InterProiView protein in InterPro
IPR037008 bc1_Rieske_TM_sf
IPR011070 Globular_prot_asu/bsu
IPR017941 Rieske_2Fe-2S
IPR036922 Rieske_2Fe-2S_sf
IPR014349 Rieske_Fe-S_prot
IPR005805 Rieske_Fe-S_prot_C
IPR004192 Rieske_TM
IPR015248 Ubiqinol_cyt_c_Rdtase_N
IPR006317 Ubiquinol_cyt_c_Rdtase_Fe-S-su
PANTHERiPTHR10134 PTHR10134, 1 hit
PfamiView protein in Pfam
PF00355 Rieske, 1 hit
PF09165 Ubiq-Cytc-red_N, 1 hit
PF02921 UCR_TM, 1 hit
PRINTSiPR00162 RIESKE
SUPFAMiSSF50022 SSF50022, 1 hit
SSF56568 SSF56568, 1 hit
TIGRFAMsiTIGR01416 Rieske_proteo, 1 hit
PROSITEiView protein in PROSITE
PS51296 RIESKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CR68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSVAARSGP FAPVLSATSR GVAGALRPLL QGAVPAASEP PVLDVKRPFL
60 70 80 90 100
CRESLSGQAA ARPLVATVGL NVPASVRFSH TDVKVPDFSD YRRAEVLDST
110 120 130 140 150
KSSKESSEAR KGFSYLVTAT TTVGVAYAAK NVVSQFVSSM SASADVLAMS
160 170 180 190 200
KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT KKEIDQEAAV EVSQLRDPQH
210 220 230 240 250
DLDRVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG SHYDASGRIR
260 270
KGPAPLNLEV PAYEFTSDDV VVVG
Length:274
Mass (Da):29,368
Last modified:June 1, 2001 - v1
Checksum:i17E55DE0BBD09961
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003966 mRNA Translation: BAB23097.1
AK012180 mRNA Translation: BAB28081.1
AK014470 mRNA Translation: BAB29374.1
AK153139 mRNA Translation: BAE31751.1
AK152391 mRNA Translation: BAE31179.1
AL611944 Genomic DNA Translation: CAI24872.1
BC019934 mRNA Translation: AAH19934.1
CCDSiCCDS26416.1
RefSeqiNP_079986.1, NM_025710.2
UniGeneiMm.181933

Genome annotation databases

EnsembliENSMUST00000042834; ENSMUSP00000045284; ENSMUSG00000038462
GeneIDi66694
KEGGimmu:66694
UCSCiuc007pyv.2 mouse

Similar proteinsi

Entry informationi

Entry nameiUCRI_MOUSE
AccessioniPrimary (citable) accession number: Q9CR68
Secondary accession number(s): Q5SVV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: June 1, 2001
Last modified: June 20, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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