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Protein

Cytochrome b-c1 complex subunit Rieske, mitochondrial

Gene

Uqcrfs1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
The transit peptide of the Rieske protein seems to form part of the bc1 complex and is considered to be the subunit 11/IX of that complex.By similarity

Catalytic activityi

Quinol + 2 ferricytochrome c = quinone + 2 ferrocytochrome c + 2 H+.

Cofactori

[2Fe-2S] clusterPROSITE-ProRule annotationNote: Binds 1 [2Fe-2S] cluster per subunit.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi217Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi219Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi236Iron-sulfur (2Fe-2S)PROSITE-ProRule annotation1
Metal bindingi239Iron-sulfur (2Fe-2S); via pros nitrogenPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b-c1 complex subunit Rieske, mitochondrial (EC:1.10.2.2)
Alternative name(s):
Complex III subunit 5
Cytochrome b-c1 complex subunit 5
Rieske iron-sulfur protein
Short name:
RISP
Ubiquinol-cytochrome c reductase iron-sulfur subunit
Cleaved into the following chain:
Alternative name(s):
Complex III subunit IX
Ubiquinol-cytochrome c reductase 8 kDa protein
Gene namesi
Name:Uqcrfs1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1913944. Uqcrfs1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei103 – 140HelicalBy similarityAdd BLAST38

GO - Cellular componenti

  • mitochondrial inner membrane Source: MGI
  • mitochondrial respiratory chain complex III Source: Ensembl
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 78MitochondrionBy similarityAdd BLAST78
ChainiPRO_00003072441 – 78Cytochrome b-c1 complex subunit 11By similarityAdd BLAST78
ChainiPRO_000003066779 – 274Cytochrome b-c1 complex subunit Rieske, mitochondrialAdd BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi222 ↔ 238PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiQ9CR68.
MaxQBiQ9CR68.
PaxDbiQ9CR68.
PeptideAtlasiQ9CR68.
PRIDEiQ9CR68.

PTM databases

iPTMnetiQ9CR68.
PhosphoSitePlusiQ9CR68.
SwissPalmiQ9CR68.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038462.
GenevisibleiQ9CR68. MM.

Interactioni

Subunit structurei

The bc1 complex contains 11 subunits: 3 respiratory subunits (cytochrome b, cytochrome c1 and Rieske/UQCRFS1), 2 core proteins (UQCRC1/QCR1 and UQCRC2/QCR2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of Rieske/UQCRFS1).By similarity

Protein-protein interaction databases

BioGridi211651. 1 interactor.
IntActiQ9CR68. 4 interactors.
MINTiMINT-1842979.
STRINGi10090.ENSMUSP00000045284.

Structurei

3D structure databases

ProteinModelPortaliQ9CR68.
SMRiQ9CR68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini187 – 272RieskePROSITE-ProRule annotationAdd BLAST86

Sequence similaritiesi

Contains 1 Rieske domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1671. Eukaryota.
COG0723. LUCA.
GeneTreeiENSGT00390000001014.
HOGENOMiHOG000255193.
HOVERGENiHBG001040.
InParanoidiQ9CR68.
KOiK00411.
OMAiPFYEFPD.
OrthoDBiEOG091G0MOP.
PhylomeDBiQ9CR68.
TreeFamiTF105037.

Family and domain databases

Gene3Di1.20.5.270. 1 hit.
2.102.10.10. 1 hit.
InterProiIPR011070. Globular_prot_asu/bsu.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR015248. Ubiqinol_cyt_c_Rdtase_N.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF00355. Rieske. 1 hit.
PF09165. Ubiq-Cytc-red_N. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF56568. SSF56568. 1 hit.
TIGRFAMsiTIGR01416. Rieske_proteo. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CR68-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSVAARSGP FAPVLSATSR GVAGALRPLL QGAVPAASEP PVLDVKRPFL
60 70 80 90 100
CRESLSGQAA ARPLVATVGL NVPASVRFSH TDVKVPDFSD YRRAEVLDST
110 120 130 140 150
KSSKESSEAR KGFSYLVTAT TTVGVAYAAK NVVSQFVSSM SASADVLAMS
160 170 180 190 200
KIEIKLSDIP EGKNMAFKWR GKPLFVRHRT KKEIDQEAAV EVSQLRDPQH
210 220 230 240 250
DLDRVKKPEW VILIGVCTHL GCVPIANAGD FGGYYCPCHG SHYDASGRIR
260 270
KGPAPLNLEV PAYEFTSDDV VVVG
Length:274
Mass (Da):29,368
Last modified:June 1, 2001 - v1
Checksum:i17E55DE0BBD09961
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003966 mRNA. Translation: BAB23097.1.
AK012180 mRNA. Translation: BAB28081.1.
AK014470 mRNA. Translation: BAB29374.1.
AK153139 mRNA. Translation: BAE31751.1.
AK152391 mRNA. Translation: BAE31179.1.
AL611944 Genomic DNA. Translation: CAI24872.1.
BC019934 mRNA. Translation: AAH19934.1.
CCDSiCCDS26416.1.
RefSeqiNP_079986.1. NM_025710.2.
UniGeneiMm.181933.

Genome annotation databases

EnsembliENSMUST00000042834; ENSMUSP00000045284; ENSMUSG00000038462.
GeneIDi66694.
KEGGimmu:66694.
UCSCiuc007pyv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003966 mRNA. Translation: BAB23097.1.
AK012180 mRNA. Translation: BAB28081.1.
AK014470 mRNA. Translation: BAB29374.1.
AK153139 mRNA. Translation: BAE31751.1.
AK152391 mRNA. Translation: BAE31179.1.
AL611944 Genomic DNA. Translation: CAI24872.1.
BC019934 mRNA. Translation: AAH19934.1.
CCDSiCCDS26416.1.
RefSeqiNP_079986.1. NM_025710.2.
UniGeneiMm.181933.

3D structure databases

ProteinModelPortaliQ9CR68.
SMRiQ9CR68.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211651. 1 interactor.
IntActiQ9CR68. 4 interactors.
MINTiMINT-1842979.
STRINGi10090.ENSMUSP00000045284.

PTM databases

iPTMnetiQ9CR68.
PhosphoSitePlusiQ9CR68.
SwissPalmiQ9CR68.

Proteomic databases

EPDiQ9CR68.
MaxQBiQ9CR68.
PaxDbiQ9CR68.
PeptideAtlasiQ9CR68.
PRIDEiQ9CR68.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042834; ENSMUSP00000045284; ENSMUSG00000038462.
GeneIDi66694.
KEGGimmu:66694.
UCSCiuc007pyv.2. mouse.

Organism-specific databases

CTDi7386.
MGIiMGI:1913944. Uqcrfs1.

Phylogenomic databases

eggNOGiKOG1671. Eukaryota.
COG0723. LUCA.
GeneTreeiENSGT00390000001014.
HOGENOMiHOG000255193.
HOVERGENiHBG001040.
InParanoidiQ9CR68.
KOiK00411.
OMAiPFYEFPD.
OrthoDBiEOG091G0MOP.
PhylomeDBiQ9CR68.
TreeFamiTF105037.

Enzyme and pathway databases

ReactomeiR-MMU-611105. Respiratory electron transport.

Miscellaneous databases

PROiQ9CR68.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038462.
GenevisibleiQ9CR68. MM.

Family and domain databases

Gene3Di1.20.5.270. 1 hit.
2.102.10.10. 1 hit.
InterProiIPR011070. Globular_prot_asu/bsu.
IPR017941. Rieske_2Fe-2S.
IPR014349. Rieske_Fe-S_prot.
IPR005805. Rieske_Fe-S_prot_C.
IPR015248. Ubiqinol_cyt_c_Rdtase_N.
IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su.
IPR004192. Ubiquinol_cyt_Rdtase_TM.
[Graphical view]
PANTHERiPTHR10134. PTHR10134. 1 hit.
PfamiPF00355. Rieske. 1 hit.
PF09165. Ubiq-Cytc-red_N. 1 hit.
PF02921. UCR_TM. 1 hit.
[Graphical view]
PRINTSiPR00162. RIESKE.
SUPFAMiSSF50022. SSF50022. 1 hit.
SSF56568. SSF56568. 1 hit.
TIGRFAMsiTIGR01416. Rieske_proteo. 1 hit.
PROSITEiPS51296. RIESKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUCRI_MOUSE
AccessioniPrimary (citable) accession number: Q9CR68
Secondary accession number(s): Q5SVV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2004
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The Rieske protein is a high potential 2Fe-2S protein.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.