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Protein

NF-kappa-B inhibitor-interacting Ras-like protein 2

Gene

Nkiras2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 188GTPBy similarity
Nucleotide bindingi61 – 655GTPBy similarity
Nucleotide bindingi120 – 1234GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. GTP catabolic process Source: InterPro
  2. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B inhibitor-interacting Ras-like protein 2
Alternative name(s):
I-kappa-B-interacting Ras-like protein 2
Short name:
Kappa B-Ras protein 2
Short name:
KappaB-Ras2
Gene namesi
Name:Nkiras2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1919216. Nkiras2.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191NF-kappa-B inhibitor-interacting Ras-like protein 2PRO_0000225680Add
BLAST

Proteomic databases

MaxQBiQ9CR56.
PaxDbiQ9CR56.
PRIDEiQ9CR56.

PTM databases

PhosphoSiteiQ9CR56.

Expressioni

Gene expression databases

BgeeiQ9CR56.
CleanExiMM_NKIRAS2.
ExpressionAtlasiQ9CR56. baseline and differential.
GenevestigatoriQ9CR56.

Interactioni

Subunit structurei

Interacts with both NF-kappa-B inhibitor alpha (NFKBIA) and beta (NFKBIB) in vitro. However, it probably only interacts with NFKBIB in vivo (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9CR56.
SMRiQ9CR56. Positions 6-155.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 191191Small GTPase-likeAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi35 – 439Effector region

Domaini

In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Ala and Leu residues, respectively, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG302793.
GeneTreeiENSGT00390000006495.
HOGENOMiHOG000230991.
HOVERGENiHBG105089.
InParanoidiQ9CR56.
KOiK17197.
OMAiIEPFVYL.
OrthoDBiEOG70KGQS.
PhylomeDBiQ9CR56.
TreeFamiTF314483.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CR56-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKSCKVVVC GQASVGKTSI LEQLLYGNHV VGSEMIETQE DIYVGSIETD
60 70 80 90 100
RGVREQVRFY DTRGLRDGAE LPKHCFSCTD GYVLVYSTDS RESFQRVELL
110 120 130 140 150
KKEIDKSKDK KEVTIVVLGN KCDLQEQRRV DPDVAQHWAK SEKVKLWEVS
160 170 180 190
VADRRSLLEP FIYLASKMTQ PQSKSAFPLS RKNKGSGSLD G
Length:191
Mass (Da):21,494
Last modified:June 1, 2001 - v1
Checksum:i1E429230C348C823
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti57 – 571V → E in BAC35078 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010371 mRNA. Translation: BAB26889.1.
AK015918 mRNA. Translation: BAB30030.1.
AK052645 mRNA. Translation: BAC35078.1.
AK083475 mRNA. Translation: BAC38929.1.
AK149696 mRNA. Translation: BAE29033.1.
AK169878 mRNA. Translation: BAE41430.1.
BC013469 mRNA. Translation: AAH13469.1.
BC024398 mRNA. Translation: AAH24398.1.
CCDSiCCDS25429.1.
RefSeqiNP_082300.1. NM_028024.2.
UniGeneiMm.274734.
Mm.485819.

Genome annotation databases

EnsembliENSMUST00000017981; ENSMUSP00000017981; ENSMUSG00000017837.
ENSMUST00000051947; ENSMUSP00000059559; ENSMUSG00000017837.
ENSMUST00000107376; ENSMUSP00000102999; ENSMUSG00000017837.
GeneIDi71966.
KEGGimmu:71966.
UCSCiuc007llt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010371 mRNA. Translation: BAB26889.1.
AK015918 mRNA. Translation: BAB30030.1.
AK052645 mRNA. Translation: BAC35078.1.
AK083475 mRNA. Translation: BAC38929.1.
AK149696 mRNA. Translation: BAE29033.1.
AK169878 mRNA. Translation: BAE41430.1.
BC013469 mRNA. Translation: AAH13469.1.
BC024398 mRNA. Translation: AAH24398.1.
CCDSiCCDS25429.1.
RefSeqiNP_082300.1. NM_028024.2.
UniGeneiMm.274734.
Mm.485819.

3D structure databases

ProteinModelPortaliQ9CR56.
SMRiQ9CR56. Positions 6-155.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9CR56.

Proteomic databases

MaxQBiQ9CR56.
PaxDbiQ9CR56.
PRIDEiQ9CR56.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017981; ENSMUSP00000017981; ENSMUSG00000017837.
ENSMUST00000051947; ENSMUSP00000059559; ENSMUSG00000017837.
ENSMUST00000107376; ENSMUSP00000102999; ENSMUSG00000017837.
GeneIDi71966.
KEGGimmu:71966.
UCSCiuc007llt.1. mouse.

Organism-specific databases

CTDi28511.
MGIiMGI:1919216. Nkiras2.

Phylogenomic databases

eggNOGiNOG302793.
GeneTreeiENSGT00390000006495.
HOGENOMiHOG000230991.
HOVERGENiHBG105089.
InParanoidiQ9CR56.
KOiK17197.
OMAiIEPFVYL.
OrthoDBiEOG70KGQS.
PhylomeDBiQ9CR56.
TreeFamiTF314483.

Miscellaneous databases

ChiTaRSiNkiras2. mouse.
NextBioi335068.
PROiQ9CR56.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CR56.
CleanExiMM_NKIRAS2.
ExpressionAtlasiQ9CR56. baseline and differential.
GenevestigatoriQ9CR56.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR020849. Small_GTPase_Ras.
[Graphical view]
PANTHERiPTHR24070. PTHR24070. 1 hit.
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and NOD.
    Tissue: Bone marrow, Kidney and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N and FVB/N-3.
    Tissue: Colon and Mammary tumor.

Entry informationi

Entry nameiKBRS2_MOUSE
AccessioniPrimary (citable) accession number: Q9CR56
Secondary accession number(s): Q8BWG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: June 1, 2001
Last modified: January 7, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.