Q9CR56 (KBRS2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NF-kappa-B inhibitor-interacting Ras-like protein 2

Alternative name(s):
I-kappa-B-interacting Ras-like protein 2
Short name=Kappa B-Ras protein 2
Short name=KappaB-Ras2

Gene names

Name:

Nkiras2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and nuclear localization of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB By similarity.
Subunit structure	Interacts with both NF-kappa-B inhibitor alpha (NFKBIA) and beta (NFKBIB) in vitro. However, it probably only interacts with NFKBIB in vivo By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	In contrast to other members of the Ras family, the members of the KappaB-Ras subfamily do not contain the conserved Gly and Gln residues in positions 13 and 65, which are replaced by Ala and Leu residues, respectively, and are therefore similar to the constitutively active forms of oncogenic forms of Ras. This suggests that members of this family are clearly different from other small GTPases proteins.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family. KappaB-Ras subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	NF-kappa-B inhibitor-interacting Ras-like protein 2		PRO_0000225680
Regions
	Nucleotide binding	11 – 18	8	GTP By similarity
	Nucleotide binding	61 – 65	5	GTP By similarity
	Nucleotide binding	120 – 123	4	GTP By similarity
	Region	1 – 191	191	Small GTPase-like
	Motif	35 – 43	9	Effector region
Experimental info
	Sequence conflict	57	1	V → E in BAC35078. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9CR56 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 1E429230C348C823	FASTA	191	21,494
	10 20 30 40 50 60 MGKSCKVVVC GQASVGKTSI LEQLLYGNHV VGSEMIETQE DIYVGSIETD RGVREQVRFY 70 80 90 100 110 120 DTRGLRDGAE LPKHCFSCTD GYVLVYSTDS RESFQRVELL KKEIDKSKDK KEVTIVVLGN 130 140 150 160 170 180 KCDLQEQRRV DPDVAQHWAK SEKVKLWEVS VADRRSLLEP FIYLASKMTQ PQSKSAFPLS 190 RKNKGSGSLD G

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Bone marrow, Kidney and Testis.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N and FVB/N-3. Tissue: Colon and Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK010371 mRNA. Translation: BAB26889.1. AK015918 mRNA. Translation: BAB30030.1. AK052645 mRNA. Translation: BAC35078.1. AK083475 mRNA. Translation: BAC38929.1. AK149696 mRNA. Translation: BAE29033.1. AK169878 mRNA. Translation: BAE41430.1. BC013469 mRNA. Translation: AAH13469.1. BC024398 mRNA. Translation: AAH24398.1.
IPI	IPI00459428.
RefSeq	NP_082300.1. NM_028024.2.
UniGene	Mm.274734. Mm.485819.
3D structure databases
ProteinModelPortal	Q9CR56.
SMR	Q9CR56. Positions 6-155.
ModBase	Search...
PTM databases
PhosphoSite	Q9CR56.
Proteomic databases
PaxDb	Q9CR56.
PRIDE	Q9CR56.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000017981; ENSMUSP00000017981; ENSMUSG00000017837. ENSMUST00000051947; ENSMUSP00000059559; ENSMUSG00000017837. ENSMUST00000107376; ENSMUSP00000102999; ENSMUSG00000017837.
GeneID	71966.
KEGG	mmu:71966.
Organism-specific databases
CTD	28511.
MGI	MGI:1919216. Nkiras2.
Phylogenomic databases
eggNOG	NOG302793.
GeneTree	ENSGT00390000006495.
HOGENOM	HOG000230991.
HOVERGEN	HBG105089.
InParanoid	Q9CR56.
OMA	IEPFVYL.
OrthoDB	EOG4RV2SH.
Gene expression databases
ArrayExpress	Q9CR56.
Bgee	Q9CR56.
CleanEx	MM_NKIRAS2.
Genevestigator	Q9CR56.
GermOnline	ENSMUSG00000017837. Mus musculus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR020849. Small_GTPase_Ras. [Graphical view]
PANTHER	PTHR24070. PTHR24070. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NKIRAS2. mouse.
NextBio	335068.
SOURCE	Search...

Entry information

Entry name

KBRS2_MOUSE

Accession

Primary (citable) accession number: Q9CR56
Secondary accession number(s): Q8BWG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program