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Protein

Chymotrypsinogen B

Gene

Ctrb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei75Charge relay systemBy similarity1
Active sitei120Charge relay systemBy similarity1
Active sitei213Charge relay systemBy similarity1

GO - Molecular functioni

  • peptidase activity Source: MGI
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  • digestion Source: UniProtKB-KW
  • proteolysis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.

Protein family/group databases

MEROPSiS01.152.

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsinogen B (EC:3.4.21.1)
Cleaved into the following 3 chains:
Gene namesi
Name:Ctrb1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:88559. Ctrb1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18By similarityAdd BLAST18
ChainiPRO_000028587419 – 263Chymotrypsinogen BAdd BLAST245
ChainiPRO_000028587519 – 31Chymotrypsin B chain ABy similarityAdd BLAST13
ChainiPRO_000028587634 – 164Chymotrypsin B chain BBy similarityAdd BLAST131
ChainiPRO_0000285877167 – 263Chymotrypsin B chain CBy similarityAdd BLAST97

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi19 ↔ 140PROSITE-ProRule annotation
Disulfide bondi60 ↔ 76PROSITE-ProRule annotation
Modified residuei93PhosphoserineCombined sources1
Disulfide bondi154 ↔ 219PROSITE-ProRule annotation
Disulfide bondi186 ↔ 200PROSITE-ProRule annotation
Disulfide bondi209 ↔ 238PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Phosphoprotein, Zymogen

Proteomic databases

MaxQBiQ9CR35.
PaxDbiQ9CR35.
PeptideAtlasiQ9CR35.
PRIDEiQ9CR35.

PTM databases

iPTMnetiQ9CR35.
PhosphoSitePlusiQ9CR35.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031957.
CleanExiMM_CTRB1.
GenevisibleiQ9CR35. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034435.

Structurei

3D structure databases

ProteinModelPortaliQ9CR35.
SMRiQ9CR35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 261Peptidase S1PROSITE-ProRule annotationAdd BLAST228

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9CR35.
KOiK01310.
OMAiHANEENI.
OrthoDBiEOG091G0AH5.
PhylomeDBiQ9CR35.
TreeFamiTF330455.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CR35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFLWLVSCF ALVGATFGCG VPAIQPVLTG LSRIVNGEDA IPGSWPWQVS
60 70 80 90 100
LQDRTGFHFC GGSLISENWV VTAAHCGVKT TDVVVAGEFD QGSDEENVQV
110 120 130 140 150
LKIAQVFKNP KFNSFTVRND ITLLKLATPA QFSETVSAVC LPTVDDDFPA
160 170 180 190 200
GTLCATTGWG KTKYNALKTP DKLQQAALPI VSEAKCKESW GSKITDVMIC
210 220 230 240 250
AGASGVSSCM GDSGGPLVCQ KDGVWTLAGI VSWGSGFCST STPAVYARVT
260
ALMPWVQEIL EAN
Length:263
Mass (Da):27,822
Last modified:June 1, 2001 - v1
Checksum:i28C4487AF1A26B27
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63S → Y in BAB25112 (PubMed:16141072).Curated1
Sequence conflicti82D → N in BAB22539 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003060 mRNA. Translation: BAB22539.1.
AK003079 mRNA. Translation: BAB22553.1.
AK007566 mRNA. Translation: BAB25112.1.
AK007765 mRNA. Translation: BAB25241.1.
AK007815 mRNA. Translation: BAB25280.1.
AK008644 mRNA. Translation: BAC25226.1.
AK008729 mRNA. Translation: BAB25861.1.
AK008888 mRNA. Translation: BAB25954.1.
AK008927 mRNA. Translation: BAB25971.1.
BC061083 mRNA. Translation: AAH61083.1.
CCDSiCCDS22679.1.
RefSeqiNP_079859.2. NM_025583.2.
UniGeneiMm.34374.

Genome annotation databases

EnsembliENSMUST00000034435; ENSMUSP00000034435; ENSMUSG00000031957.
GeneIDi66473.
KEGGimmu:66473.
UCSCiuc009nms.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003060 mRNA. Translation: BAB22539.1.
AK003079 mRNA. Translation: BAB22553.1.
AK007566 mRNA. Translation: BAB25112.1.
AK007765 mRNA. Translation: BAB25241.1.
AK007815 mRNA. Translation: BAB25280.1.
AK008644 mRNA. Translation: BAC25226.1.
AK008729 mRNA. Translation: BAB25861.1.
AK008888 mRNA. Translation: BAB25954.1.
AK008927 mRNA. Translation: BAB25971.1.
BC061083 mRNA. Translation: AAH61083.1.
CCDSiCCDS22679.1.
RefSeqiNP_079859.2. NM_025583.2.
UniGeneiMm.34374.

3D structure databases

ProteinModelPortaliQ9CR35.
SMRiQ9CR35.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034435.

Protein family/group databases

MEROPSiS01.152.

PTM databases

iPTMnetiQ9CR35.
PhosphoSitePlusiQ9CR35.

Proteomic databases

MaxQBiQ9CR35.
PaxDbiQ9CR35.
PeptideAtlasiQ9CR35.
PRIDEiQ9CR35.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034435; ENSMUSP00000034435; ENSMUSG00000031957.
GeneIDi66473.
KEGGimmu:66473.
UCSCiuc009nms.2. mouse.

Organism-specific databases

CTDi1504.
MGIiMGI:88559. Ctrb1.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000119027.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9CR35.
KOiK01310.
OMAiHANEENI.
OrthoDBiEOG091G0AH5.
PhylomeDBiQ9CR35.
TreeFamiTF330455.

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.

Miscellaneous databases

ChiTaRSiCtrb1. mouse.
PROiQ9CR35.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031957.
CleanExiMM_CTRB1.
GenevisibleiQ9CR35. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTRB1_MOUSE
AccessioniPrimary (citable) accession number: Q9CR35
Secondary accession number(s): Q9D8X8, Q9DC86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: June 1, 2001
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.