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Protein

Josephin-2

Gene

Josd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves 'Lys-63'-linked poly-ubiquitin chains, and with lesser efficiency 'Lys-48'-linked poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei24NucleophilePROSITE-ProRule annotation1
Active sitei125Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-5689877. Josephin domain DUBs.

Protein family/group databases

MEROPSiC86.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Josephin-2 (EC:3.4.19.12)
Alternative name(s):
Josephin domain-containing protein 2
Gene namesi
Name:Josd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1913374. Josd2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000538441 – 188Josephin-2Add BLAST188

Proteomic databases

PaxDbiQ9CR30.
PRIDEiQ9CR30.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038695.
CleanExiMM_JOSD2.
ExpressionAtlasiQ9CR30. baseline and differential.
GenevisibleiQ9CR30. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048415.

Structurei

3D structure databases

ProteinModelPortaliQ9CR30.
SMRiQ9CR30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 188JosephinPROSITE-ProRule annotationAdd BLAST178

Sequence similaritiesi

Contains 1 Josephin domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2934. Eukaryota.
ENOG4111I4J. LUCA.
GeneTreeiENSGT00390000009228.
HOGENOMiHOG000005731.
HOVERGENiHBG039523.
InParanoidiQ9CR30.
KOiK15235.
OMAiAEDGMSQ.
OrthoDBiEOG091G0NAU.
PhylomeDBiQ9CR30.
TreeFamiTF313660.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
SMARTiSM01246. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CR30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQAPEARPS PPSVYHERQR LELCAVHALN NVLQEQLFSQ EAADEICKRL
60 70 80 90 100
APDSRLNPHR SLLGTGNYDV NVIMAALQGL GLAAVWWDRR RPLSQLALPQ
110 120 130 140 150
VLGLILNLPS PVSLGLLSLP LRRRHWVALR QVDGIYYNLD SKLRAPEALG
160 170 180
DEDGVRTFLA AALAQGLCEV LLVVTKEVEE AGCWLNTS
Length:188
Mass (Da):20,776
Last modified:June 1, 2001 - v1
Checksum:i2B2DE4BB1E9243F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003525 mRNA. Translation: BAB22837.1.
AK002872 mRNA. Translation: BAB22420.1.
CCDSiCCDS21208.1.
RefSeqiNP_001191999.1. NM_001205070.1.
NP_001192000.1. NM_001205071.1.
NP_001192001.1. NM_001205072.1.
NP_001192002.1. NM_001205073.1.
NP_079644.1. NM_025368.4.
XP_006541137.1. XM_006541074.2.
UniGeneiMm.358691.

Genome annotation databases

EnsembliENSMUST00000035844; ENSMUSP00000048415; ENSMUSG00000038695.
ENSMUST00000117324; ENSMUSP00000113313; ENSMUSG00000038695.
ENSMUST00000118493; ENSMUSP00000113226; ENSMUSG00000038695.
ENSMUST00000118628; ENSMUSP00000113172; ENSMUSG00000038695.
ENSMUST00000120852; ENSMUSP00000114105; ENSMUSG00000038695.
GeneIDi66124.
KEGGimmu:66124.
UCSCiuc009gpl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003525 mRNA. Translation: BAB22837.1.
AK002872 mRNA. Translation: BAB22420.1.
CCDSiCCDS21208.1.
RefSeqiNP_001191999.1. NM_001205070.1.
NP_001192000.1. NM_001205071.1.
NP_001192001.1. NM_001205072.1.
NP_001192002.1. NM_001205073.1.
NP_079644.1. NM_025368.4.
XP_006541137.1. XM_006541074.2.
UniGeneiMm.358691.

3D structure databases

ProteinModelPortaliQ9CR30.
SMRiQ9CR30.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000048415.

Protein family/group databases

MEROPSiC86.005.

Proteomic databases

PaxDbiQ9CR30.
PRIDEiQ9CR30.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035844; ENSMUSP00000048415; ENSMUSG00000038695.
ENSMUST00000117324; ENSMUSP00000113313; ENSMUSG00000038695.
ENSMUST00000118493; ENSMUSP00000113226; ENSMUSG00000038695.
ENSMUST00000118628; ENSMUSP00000113172; ENSMUSG00000038695.
ENSMUST00000120852; ENSMUSP00000114105; ENSMUSG00000038695.
GeneIDi66124.
KEGGimmu:66124.
UCSCiuc009gpl.2. mouse.

Organism-specific databases

CTDi126119.
MGIiMGI:1913374. Josd2.

Phylogenomic databases

eggNOGiKOG2934. Eukaryota.
ENOG4111I4J. LUCA.
GeneTreeiENSGT00390000009228.
HOGENOMiHOG000005731.
HOVERGENiHBG039523.
InParanoidiQ9CR30.
KOiK15235.
OMAiAEDGMSQ.
OrthoDBiEOG091G0NAU.
PhylomeDBiQ9CR30.
TreeFamiTF313660.

Enzyme and pathway databases

ReactomeiR-MMU-5689877. Josephin domain DUBs.

Miscellaneous databases

PROiQ9CR30.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038695.
CleanExiMM_JOSD2.
ExpressionAtlasiQ9CR30. baseline and differential.
GenevisibleiQ9CR30. MM.

Family and domain databases

InterProiIPR006155. Josephin.
[Graphical view]
PfamiPF02099. Josephin. 1 hit.
[Graphical view]
SMARTiSM01246. Josephin. 1 hit.
[Graphical view]
PROSITEiPS50957. JOSEPHIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJOS2_MOUSE
AccessioniPrimary (citable) accession number: Q9CR30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.