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Protein

E3 ubiquitin-protein ligase FANCL

Gene

Fancl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri307 – 36559RING-typeAdd
BLAST

GO - Molecular functioni

  • ligase activity Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin protein ligase binding Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB
  • DNA repair Source: UniProtKB
  • gamete generation Source: MGI
  • positive regulation of defense response to virus by host Source: MGI
  • protein monoubiquitination Source: UniProtKB
  • regulation of cell proliferation Source: MGI
  • xenophagy Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6783310. Fanconi Anemia Pathway.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase FANCL (EC:6.3.2.-)
Alternative name(s):
Fanconi anemia group L protein homolog
Proliferation of germ cells protein
Gene namesi
Name:Fancl
Synonyms:Phf9, Pog
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914280. Fancl.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • Fanconi anaemia nuclear complex Source: UniProtKB
  • nuclear envelope Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Fancl are a cause of the gcd (germ cell deficient) mutant phenotype, which leads to reduced numbers of precursor germ cells and adult sterility, probably due to a reduced precursor germ cells proliferation.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 375374E3 ubiquitin-protein ligase FANCLPRO_0000055909Add
BLAST

Post-translational modificationi

The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ9CR14.
PaxDbiQ9CR14.
PRIDEiQ9CR14.

PTM databases

PhosphoSiteiQ9CR14.

Expressioni

Gene expression databases

BgeeiENSMUSG00000004018.
CleanExiMM_FANCL.
ExpressionAtlasiQ9CR14. baseline and differential.
GenevisibleiQ9CR14. MM.

Interactioni

Subunit structurei

Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM (By similarity). In complex with FANCF, FANCA and FANCG, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. Interacts with FANCI (By similarity). Interacts with GGN. Interacts (via the RING-type zinc finger) with UBE2T and UBE2W.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211887. 8 interactions.
IntActiQ9CR14. 3 interactions.
MINTiMINT-1341363.
STRINGi10090.ENSMUSP00000004120.

Structurei

3D structure databases

ProteinModelPortaliQ9CR14.
SMRiQ9CR14. Positions 74-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni104 – 294191UBC-RWD region (URD)By similarityAdd
BLAST

Domaini

The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.By similarity

Sequence similaritiesi

Contains 1 RING-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri307 – 36559RING-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3268. Eukaryota.
ENOG410YC2U. LUCA.
GeneTreeiENSGT00390000005537.
HOGENOMiHOG000007643.
HOVERGENiHBG045632.
InParanoidiQ9CR14.
KOiK10606.
OMAiPKMLPEC.
OrthoDBiEOG091G0PRR.
PhylomeDBiQ9CR14.
TreeFamiTF323571.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR026848. Fancl.
IPR026850. FANCL_C.
IPR019162. FancL_WD-rpt_cont_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR13206:SF0. PTHR13206:SF0. 1 hit.
PfamiPF11793. FANCL_C. 1 hit.
PF09765. WD-3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CR14-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEAEASLLR HFPLLLPQNR EKTVYEGFIS AQGSDFHLRI VLPKDLQLKK
60 70 80 90 100
ARLLCSLQLK NILNEYHQVV QQRMKHSPDL MSFMMELKMI LEVALKNKQE
110 120 130 140 150
LCVQPPSCSF CKDLLTEIGA IGWDKLACVE SSFSTIKLKA DDASGRKHLI
160 170 180 190 200
TVKLKAKYPV EPPDCVVDFP VPFSVSWTPQ SSLVDVYSQF LVALETLKVF
210 220 230 240 250
WDVMDEIDEK TWVLEPEKPP RSATARRIAL GKNVSIAIEV DPRHPTMLPE
260 270 280 290 300
FCFLGADHVT KPLGMKLSGS IHLWDPENSL LQNLKDVLEI DFPARSILEE
310 320 330 340 350
SDFSMDCGIC YARHLNGAIP DQVCNNPQCG QPFHEICLYE WLRGLSTSRQ
360 370
SFNVFFGDCP YCSKPITLKM SGRKP
Length:375
Mass (Da):42,527
Last modified:June 1, 2001 - v1
Checksum:i33CE1FE964E917DA
GO
Isoform 2 (identifier: Q9CR14-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     274-279: WDPENS → C

Note: No experimental confirmation available.
Show »
Length:370
Mass (Da):41,902
Checksum:i7DC016A9D404BFF1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti230 – 2301L → I in BAB27906 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei274 – 2796WDPENS → C in isoform 2. 1 PublicationVSP_008552

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF513619 mRNA. Translation: AAN64921.1.
AK008605 mRNA. Translation: BAB25772.1.
AK011520 mRNA. Translation: BAB27674.1.
AK011752 mRNA. Translation: BAB27820.1.
AK011903 mRNA. Translation: BAB27906.1.
AK018018 mRNA. Translation: BAB31039.1.
AK076017 mRNA. Translation: BAC36123.1.
AK168538 mRNA. Translation: BAE40416.1.
BC066181 mRNA. Translation: AAH66181.1.
BC032876 mRNA. Translation: AAH32876.1.
CCDSiCCDS24485.1. [Q9CR14-1]
CCDS70152.1. [Q9CR14-2]
RefSeqiNP_001264202.1. NM_001277273.1. [Q9CR14-2]
NP_080199.1. NM_025923.3. [Q9CR14-1]
UniGeneiMm.18875.

Genome annotation databases

EnsembliENSMUST00000004120; ENSMUSP00000004120; ENSMUSG00000004018. [Q9CR14-1]
ENSMUST00000109509; ENSMUSP00000105135; ENSMUSG00000004018. [Q9CR14-2]
GeneIDi67030.
KEGGimmu:67030.
UCSCiuc007ige.2. mouse. [Q9CR14-1]
uc011xsj.2. mouse. [Q9CR14-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF513619 mRNA. Translation: AAN64921.1.
AK008605 mRNA. Translation: BAB25772.1.
AK011520 mRNA. Translation: BAB27674.1.
AK011752 mRNA. Translation: BAB27820.1.
AK011903 mRNA. Translation: BAB27906.1.
AK018018 mRNA. Translation: BAB31039.1.
AK076017 mRNA. Translation: BAC36123.1.
AK168538 mRNA. Translation: BAE40416.1.
BC066181 mRNA. Translation: AAH66181.1.
BC032876 mRNA. Translation: AAH32876.1.
CCDSiCCDS24485.1. [Q9CR14-1]
CCDS70152.1. [Q9CR14-2]
RefSeqiNP_001264202.1. NM_001277273.1. [Q9CR14-2]
NP_080199.1. NM_025923.3. [Q9CR14-1]
UniGeneiMm.18875.

3D structure databases

ProteinModelPortaliQ9CR14.
SMRiQ9CR14. Positions 74-373.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211887. 8 interactions.
IntActiQ9CR14. 3 interactions.
MINTiMINT-1341363.
STRINGi10090.ENSMUSP00000004120.

PTM databases

PhosphoSiteiQ9CR14.

Proteomic databases

MaxQBiQ9CR14.
PaxDbiQ9CR14.
PRIDEiQ9CR14.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000004120; ENSMUSP00000004120; ENSMUSG00000004018. [Q9CR14-1]
ENSMUST00000109509; ENSMUSP00000105135; ENSMUSG00000004018. [Q9CR14-2]
GeneIDi67030.
KEGGimmu:67030.
UCSCiuc007ige.2. mouse. [Q9CR14-1]
uc011xsj.2. mouse. [Q9CR14-2]

Organism-specific databases

CTDi55120.
MGIiMGI:1914280. Fancl.

Phylogenomic databases

eggNOGiKOG3268. Eukaryota.
ENOG410YC2U. LUCA.
GeneTreeiENSGT00390000005537.
HOGENOMiHOG000007643.
HOVERGENiHBG045632.
InParanoidiQ9CR14.
KOiK10606.
OMAiPKMLPEC.
OrthoDBiEOG091G0PRR.
PhylomeDBiQ9CR14.
TreeFamiTF323571.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-6783310. Fanconi Anemia Pathway.

Miscellaneous databases

ChiTaRSiFancl. mouse.
PROiQ9CR14.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000004018.
CleanExiMM_FANCL.
ExpressionAtlasiQ9CR14. baseline and differential.
GenevisibleiQ9CR14. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR026848. Fancl.
IPR026850. FANCL_C.
IPR019162. FancL_WD-rpt_cont_dom.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PANTHERiPTHR13206:SF0. PTHR13206:SF0. 1 hit.
PfamiPF11793. FANCL_C. 1 hit.
PF09765. WD-3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFANCL_MOUSE
AccessioniPrimary (citable) accession number: Q9CR14
Secondary accession number(s): Q3TGY2, Q9D017
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: June 1, 2001
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Despite its name, it does not contain a PHD-type zinc finger, but contains a RING-type zinc finger. Moreover, PHD-type zinc fingers do not have any ubiquitin ligase activity.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.