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Protein

E3 ubiquitin-protein ligase FANCL

Gene

Fancl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.2 Publications

Caution

Despite its name, it does not contain a PHD-type zinc finger, but contains a RING-type zinc finger. Moreover, PHD-type zinc fingers do not have any ubiquitin ligase activity.Curated

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri307 – 365RING-typeAdd BLAST59

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin protein ligase binding Source: MGI
  • ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  • cellular response to DNA damage stimulus Source: UniProtKB
  • DNA repair Source: UniProtKB
  • gamete generation Source: MGI
  • protein monoubiquitination Source: UniProtKB
  • regulation of cell proliferation Source: MGI

Keywordsi

Molecular functionTransferase
Biological processDNA damage, DNA repair, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6783310 Fanconi Anemia Pathway
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase FANCL (EC:2.3.2.27By similarity)
Alternative name(s):
Fanconi anemia group L protein homolog
Proliferation of germ cells protein
RING-type E3 ubiquitin transferase FANCLCurated
Gene namesi
Name:Fancl
Synonyms:Phf9, Pog
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914280 Fancl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Involvement in diseasei

Defects in Fancl are a cause of the gcd (germ cell deficient) mutant phenotype, which leads to reduced numbers of precursor germ cells and adult sterility, probably due to a reduced precursor germ cells proliferation.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000559091 – 375E3 ubiquitin-protein ligase FANCLAdd BLAST375

Post-translational modificationi

The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ9CR14
PRIDEiQ9CR14

PTM databases

PhosphoSitePlusiQ9CR14

Expressioni

Gene expression databases

BgeeiENSMUSG00000004018
CleanExiMM_FANCL
ExpressionAtlasiQ9CR14 baseline and differential
GenevisibleiQ9CR14 MM

Interactioni

Subunit structurei

Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM (By similarity). In complex with FANCF, FANCA and FANCG, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. Interacts with FANCI (By similarity). Interacts with GGN. Interacts (via the RING-type zinc finger) with UBE2T and UBE2W.By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211887, 9 interactors
IntActiQ9CR14, 3 interactors
MINTiQ9CR14
STRINGi10090.ENSMUSP00000004120

Structurei

3D structure databases

ProteinModelPortaliQ9CR14
SMRiQ9CR14
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni104 – 294UBC-RWD region (URD)By similarityAdd BLAST191

Domaini

The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri307 – 365RING-typeAdd BLAST59

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3268 Eukaryota
ENOG410YC2U LUCA
GeneTreeiENSGT00390000005537
HOGENOMiHOG000007643
HOVERGENiHBG045632
InParanoidiQ9CR14
KOiK10606
OMAiWTPQSSL
OrthoDBiEOG091G0PRR
PhylomeDBiQ9CR14
TreeFamiTF323571

Family and domain databases

Gene3Di3.10.110.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR026848 Fancl
IPR026850 FANCL_C
IPR019162 FancL_WD-rpt_cont_dom
IPR016135 UBQ-conjugating_enzyme/RWD
IPR013083 Znf_RING/FYVE/PHD
PANTHERiPTHR13206 PTHR13206, 1 hit
PfamiView protein in Pfam
PF11793 FANCL_C, 1 hit
PF09765 WD-3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9CR14-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDEAEASLLR HFPLLLPQNR EKTVYEGFIS AQGSDFHLRI VLPKDLQLKK
60 70 80 90 100
ARLLCSLQLK NILNEYHQVV QQRMKHSPDL MSFMMELKMI LEVALKNKQE
110 120 130 140 150
LCVQPPSCSF CKDLLTEIGA IGWDKLACVE SSFSTIKLKA DDASGRKHLI
160 170 180 190 200
TVKLKAKYPV EPPDCVVDFP VPFSVSWTPQ SSLVDVYSQF LVALETLKVF
210 220 230 240 250
WDVMDEIDEK TWVLEPEKPP RSATARRIAL GKNVSIAIEV DPRHPTMLPE
260 270 280 290 300
FCFLGADHVT KPLGMKLSGS IHLWDPENSL LQNLKDVLEI DFPARSILEE
310 320 330 340 350
SDFSMDCGIC YARHLNGAIP DQVCNNPQCG QPFHEICLYE WLRGLSTSRQ
360 370
SFNVFFGDCP YCSKPITLKM SGRKP
Length:375
Mass (Da):42,527
Last modified:June 1, 2001 - v1
Checksum:i33CE1FE964E917DA
GO
Isoform 2 (identifier: Q9CR14-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     274-279: WDPENS → C

Note: No experimental confirmation available.
Show »
Length:370
Mass (Da):41,902
Checksum:i7DC016A9D404BFF1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti230L → I in BAB27906 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_008552274 – 279WDPENS → C in isoform 2. 1 Publication6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF513619 mRNA Translation: AAN64921.1
AK008605 mRNA Translation: BAB25772.1
AK011520 mRNA Translation: BAB27674.1
AK011752 mRNA Translation: BAB27820.1
AK011903 mRNA Translation: BAB27906.1
AK018018 mRNA Translation: BAB31039.1
AK076017 mRNA Translation: BAC36123.1
AK168538 mRNA Translation: BAE40416.1
BC066181 mRNA Translation: AAH66181.1
BC032876 mRNA Translation: AAH32876.1
CCDSiCCDS24485.1 [Q9CR14-1]
CCDS70152.1 [Q9CR14-2]
RefSeqiNP_001264202.1, NM_001277273.1 [Q9CR14-2]
NP_080199.1, NM_025923.3 [Q9CR14-1]
UniGeneiMm.18875

Genome annotation databases

EnsembliENSMUST00000004120; ENSMUSP00000004120; ENSMUSG00000004018 [Q9CR14-1]
ENSMUST00000109509; ENSMUSP00000105135; ENSMUSG00000004018 [Q9CR14-2]
GeneIDi67030
KEGGimmu:67030
UCSCiuc007ige.2 mouse [Q9CR14-1]
uc011xsj.2 mouse [Q9CR14-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFANCL_MOUSE
AccessioniPrimary (citable) accession number: Q9CR14
Secondary accession number(s): Q3TGY2, Q9D017
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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