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Q9CQY1

- ATG12_MOUSE

UniProt

Q9CQY1 - ATG12_MOUSE

Protein

Ubiquitin-like protein ATG12

Gene

Atg12

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.3 Publications

    GO - Molecular functioni

    1. Atg8 ligase activity Source: RefGenome
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. autophagic vacuole assembly Source: UniProtKB
    2. autophagy Source: MGI
    3. cellular response to nitrogen starvation Source: RefGenome
    4. C-terminal protein lipidation Source: RefGenome
    5. innate immune response Source: UniProtKB-KW
    6. mitochondrion degradation Source: RefGenome
    7. nucleophagy Source: RefGenome

    Keywords - Biological processi

    Autophagy, Immunity, Innate immunity, Ubl conjugation pathway

    Enzyme and pathway databases

    ReactomeiREACT_198532. Negative regulators of RIG-I/MDA5 signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-like protein ATG12
    Alternative name(s):
    Autophagy-related protein 12
    Short name:
    APG12-like
    Gene namesi
    Name:Atg12
    Synonyms:Apg12, Apg12l
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1914776. Atg12.

    Subcellular locationi

    Cytoplasm By similarity. Preautophagosomal structure membrane By similarity; Peripheral membrane protein By similarity
    Note: TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.By similarity

    GO - Cellular componenti

    1. Atg12-Atg5-Atg16 complex Source: RefGenome
    2. cytosol Source: Reactome
    3. pre-autophagosomal structure membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 141141Ubiquitin-like protein ATG12PRO_0000212472Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki141 – 141Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein)

    Post-translational modificationi

    Acetylated by EP300.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond

    Proteomic databases

    PaxDbiQ9CQY1.
    PRIDEiQ9CQY1.

    PTM databases

    PhosphoSiteiQ9CQY1.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    BgeeiQ9CQY1.
    CleanExiMM_ATG12.
    GenevestigatoriQ9CQY1.

    Interactioni

    Subunit structurei

    Forms a conjugate with ATG5. The ATG12-ATG5 conjugate forms a complex with several units of ATG16L. Interacts with ATG3, ATG7 and ATG10. ATG12-ATG5 also interacts with MAVS, MGA, RARRES3 and TECPR1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Atg3Q9CPX63EBI-2911788,EBI-2911810
    Atg5Q99J833EBI-2911788,EBI-2911848

    Protein-protein interaction databases

    BioGridi212249. 4 interactions.
    IntActiQ9CQY1. 8 interactions.
    MINTiMINT-4613690.
    STRINGi10090.ENSMUSP00000038489.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CQY1.
    SMRiQ9CQY1. Positions 54-141.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.1 Publication

    Sequence similaritiesi

    Belongs to the ATG12 family.Curated

    Phylogenomic databases

    eggNOGiNOG236266.
    GeneTreeiENSGT00390000016654.
    HOGENOMiHOG000234863.
    HOVERGENiHBG080875.
    InParanoidiQ9CQY1.
    KOiK08336.
    OMAiPWRKARP.
    OrthoDBiEOG7XPZ7X.
    PhylomeDBiQ9CQY1.
    TreeFamiTF325131.

    Family and domain databases

    InterProiIPR007242. Atg12.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PANTHERiPTHR13385. PTHR13385. 1 hit.
    PfamiPF04110. APG12. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9CQY1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEDSEVVLQ LPSAPVGAGG ESLPELSPET ATPEPPSSAA VSPGTEEPPG    50
    DTKKKIDILL KAVGDTPIMK TKKWAVERTR TIQGLIDFIK KFLKLVASEQ 100
    LFIYVNQSFA PSPDQEVGTL YECFGSDGKL VLHYCKSQAW G 141
    Length:141
    Mass (Da):15,207
    Last modified:June 1, 2001 - v1
    Checksum:i440F9A625624A78B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti44 – 485GTEEP → ERGT in BAB25839. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB066216 mRNA. Translation: BAB62092.1.
    AK008698 mRNA. Translation: BAB25839.1.
    AK016474 mRNA. Translation: BAB30256.1.
    AK005405 mRNA. Translation: BAB24005.1.
    AK167027 mRNA. Translation: BAE39200.1.
    BC070470 mRNA. Translation: AAH70470.1.
    CCDSiCCDS37811.1.
    RefSeqiNP_080493.2. NM_026217.3.
    UniGeneiMm.9852.

    Genome annotation databases

    EnsembliENSMUST00000035648; ENSMUSP00000038489; ENSMUSG00000032905.
    GeneIDi67526.
    KEGGimmu:67526.
    UCSCiuc008evu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB066216 mRNA. Translation: BAB62092.1 .
    AK008698 mRNA. Translation: BAB25839.1 .
    AK016474 mRNA. Translation: BAB30256.1 .
    AK005405 mRNA. Translation: BAB24005.1 .
    AK167027 mRNA. Translation: BAE39200.1 .
    BC070470 mRNA. Translation: AAH70470.1 .
    CCDSi CCDS37811.1.
    RefSeqi NP_080493.2. NM_026217.3.
    UniGenei Mm.9852.

    3D structure databases

    ProteinModelPortali Q9CQY1.
    SMRi Q9CQY1. Positions 54-141.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 212249. 4 interactions.
    IntActi Q9CQY1. 8 interactions.
    MINTi MINT-4613690.
    STRINGi 10090.ENSMUSP00000038489.

    PTM databases

    PhosphoSitei Q9CQY1.

    Proteomic databases

    PaxDbi Q9CQY1.
    PRIDEi Q9CQY1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000035648 ; ENSMUSP00000038489 ; ENSMUSG00000032905 .
    GeneIDi 67526.
    KEGGi mmu:67526.
    UCSCi uc008evu.1. mouse.

    Organism-specific databases

    CTDi 9140.
    MGIi MGI:1914776. Atg12.

    Phylogenomic databases

    eggNOGi NOG236266.
    GeneTreei ENSGT00390000016654.
    HOGENOMi HOG000234863.
    HOVERGENi HBG080875.
    InParanoidi Q9CQY1.
    KOi K08336.
    OMAi PWRKARP.
    OrthoDBi EOG7XPZ7X.
    PhylomeDBi Q9CQY1.
    TreeFami TF325131.

    Enzyme and pathway databases

    Reactomei REACT_198532. Negative regulators of RIG-I/MDA5 signaling.

    Miscellaneous databases

    ChiTaRSi ATG12. mouse.
    NextBioi 324820.
    PROi Q9CQY1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9CQY1.
    CleanExi MM_ATG12.
    Genevestigatori Q9CQY1.

    Family and domain databases

    InterProi IPR007242. Atg12.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    PANTHERi PTHR13385. PTHR13385. 1 hit.
    Pfami PF04110. APG12. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells."
      Mizushima N., Yamamoto A., Hatano M., Kobayashi Y., Kabeya Y., Suzuki K., Tokuhisa T., Ohsumi Y., Yoshimori T.
      J. Cell Biol. 152:657-668(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CONJUGATION TO ATG5, FUNCTION OF THE ATG12/ATG5 CONJUGATE, SUBCELLULAR LOCATION.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Blastocyst, Placenta, Stomach and Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Mouse Apg10 as an Apg12-conjugating enzyme: analysis by the conjugation-mediated yeast two-hybrid method."
      Mizushima N., Yoshimori T., Ohsumi Y.
      FEBS Lett. 532:450-454(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG10, CONJUGATION TO ATG5.
    5. "The mouse APG10 homologue, an E2-like enzyme for Apg12p conjugation, facilitates MAP-LC3 modification."
      Nemoto T., Tanida I., Tanida-Miyake E., Minematsu-Ikeguchi N., Yokota M., Ohsumi M., Ueno T., Kominami E.
      J. Biol. Chem. 278:39517-39526(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CONJUGATION TO ATG5 BY ATG10, FUNCTION.
    6. "Mouse Apg16L, a novel WD-repeat protein, targets to the autophagic isolation membrane with the Apg12-Apg5 conjugate."
      Mizushima N., Kuma A., Kobayashi Y., Yamamoto A., Matsubae M., Takao T., Natsume T., Ohsumi Y., Yoshimori T.
      J. Cell Sci. 116:1679-1688(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN A COMPLEX WITH ATG5 AND ATG16.
    7. "The Atg8 and Atg12 ubiquitin-like conjugation systems in macroautophagy. 'Protein modifications: beyond the usual suspects' review series."
      Geng J., Klionsky D.J.
      EMBO Rep. 9:859-864(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN.
    8. "The Atg8 conjugation system is indispensable for proper development of autophagic isolation membranes in mice."
      Sou Y.S., Waguri S., Iwata J., Ueno T., Fujimura T., Hara T., Sawada N., Yamada A., Mizushima N., Uchiyama Y., Kominami E., Tanaka K., Komatsu M.
      Mol. Biol. Cell 19:4762-4775(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: CONJUGATION TO ATG5.
    9. "Mitochondrial clearance is regulated by Atg7-dependent and -independent mechanisms during reticulocyte maturation."
      Zhang J., Randall M.S., Loyd M.R., Dorsey F.C., Kundu M., Cleveland J.L., Ney P.A.
      Blood 114:157-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: CONJUGATION TO ATG5.
    10. "ATG12 conjugation to ATG3 regulates mitochondrial homeostasis and cell death."
      Radoshevich L., Murrow L., Chen N., Fernandez E., Roy S., Fung C., Debnath J.
      Cell 142:590-600(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiATG12_MOUSE
    AccessioniPrimary (citable) accession number: Q9CQY1
    Secondary accession number(s): Q3TKE5, Q9D7Y5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3