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Protein

Cytochrome b5 type B

Gene

Cyb5b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi79Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b5 type B
Alternative name(s):
Cytochrome b5 outer mitochondrial membrane isoform
Gene namesi
Name:Cyb5b
Synonyms:Cyb5m
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1913677. Cyb5b.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei119 – 136HelicalSequence analysisAdd BLAST18

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: MGI
  • mitochondrial inner membrane Source: MGI
  • mitochondrial outer membrane Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
PropeptideiPRO_00000064732 – 11By similarity10
ChainiPRO_000000647412 – 146Cytochrome b5 type BAdd BLAST135

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei19PhosphoserineBy similarity1
Modified residuei30N6-acetyllysineBy similarity1
Modified residuei33PhosphoserineCombined sources1
Modified residuei80PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9CQX2.
MaxQBiQ9CQX2.
PaxDbiQ9CQX2.
PRIDEiQ9CQX2.
TopDownProteomicsiQ9CQX2.

PTM databases

iPTMnetiQ9CQX2.
PhosphoSitePlusiQ9CQX2.
SwissPalmiQ9CQX2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000031924.
CleanExiMM_CYB5B.
GenevisibleiQ9CQX2. MM.

Interactioni

Subunit structurei

Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2.By similarity

Protein-protein interaction databases

IntActiQ9CQX2. 3 interactors.
MINTiMINT-4125004.
STRINGi10090.ENSMUSP00000034400.

Structurei

3D structure databases

ProteinModelPortaliQ9CQX2.
SMRiQ9CQX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 96Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST77

Sequence similaritiesi

Belongs to the cytochrome b5 family.Curated
Contains 1 cytochrome b5 heme-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiQ9CQX2.
OMAiYYMAESK.
OrthoDBiEOG091G17G8.
PhylomeDBiQ9CQX2.
TreeFamiTF314537.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
3.10.120.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CQX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPEASGSG EKVEGSEPSV TYYRLEEVAK RNSAEETWMV IHGRVYDITR
60 70 80 90 100
FLSEHPGGEE VLLEQAGADA TESFEDVGHS PDAREMLKQY YIGDVHPSDL
110 120 130 140
KPKGDDKDPS KNNSCQSSWA YWFVPIVGAI LIGFLYRHFW ADSKSS
Length:146
Mass (Da):16,318
Last modified:June 1, 2001 - v1
Checksum:i4ADA7C96A5F311DD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti31R → E in BAB25251 (PubMed:16141072).Curated1
Sequence conflicti142D → G in BAB22721 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003333 mRNA. Translation: BAB22721.1.
AK003815 mRNA. Translation: BAB23012.1.
AK007780 mRNA. Translation: BAB25251.1.
AK019263 mRNA. Translation: BAB31635.1.
AK052810 mRNA. Translation: BAC35156.1.
AK088970 mRNA. Translation: BAC40677.1.
BC054749 mRNA. Translation: AAH54749.1.
BC058812 mRNA. Translation: AAH58812.1.
BC062980 mRNA. Translation: AAH62980.1.
CCDSiCCDS22647.1.
RefSeqiNP_079834.2. NM_025558.5.
UniGeneiMm.20242.

Genome annotation databases

EnsembliENSMUST00000034400; ENSMUSP00000034400; ENSMUSG00000031924.
GeneIDi66427.
KEGGimmu:66427.
UCSCiuc009nhg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK003333 mRNA. Translation: BAB22721.1.
AK003815 mRNA. Translation: BAB23012.1.
AK007780 mRNA. Translation: BAB25251.1.
AK019263 mRNA. Translation: BAB31635.1.
AK052810 mRNA. Translation: BAC35156.1.
AK088970 mRNA. Translation: BAC40677.1.
BC054749 mRNA. Translation: AAH54749.1.
BC058812 mRNA. Translation: AAH58812.1.
BC062980 mRNA. Translation: AAH62980.1.
CCDSiCCDS22647.1.
RefSeqiNP_079834.2. NM_025558.5.
UniGeneiMm.20242.

3D structure databases

ProteinModelPortaliQ9CQX2.
SMRiQ9CQX2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CQX2. 3 interactors.
MINTiMINT-4125004.
STRINGi10090.ENSMUSP00000034400.

PTM databases

iPTMnetiQ9CQX2.
PhosphoSitePlusiQ9CQX2.
SwissPalmiQ9CQX2.

Proteomic databases

EPDiQ9CQX2.
MaxQBiQ9CQX2.
PaxDbiQ9CQX2.
PRIDEiQ9CQX2.
TopDownProteomicsiQ9CQX2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034400; ENSMUSP00000034400; ENSMUSG00000031924.
GeneIDi66427.
KEGGimmu:66427.
UCSCiuc009nhg.1. mouse.

Organism-specific databases

CTDi80777.
MGIiMGI:1913677. Cyb5b.

Phylogenomic databases

eggNOGiKOG0537. Eukaryota.
COG5274. LUCA.
GeneTreeiENSGT00510000046507.
HOGENOMiHOG000039853.
HOVERGENiHBG002653.
InParanoidiQ9CQX2.
OMAiYYMAESK.
OrthoDBiEOG091G17G8.
PhylomeDBiQ9CQX2.
TreeFamiTF314537.

Miscellaneous databases

PROiQ9CQX2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031924.
CleanExiMM_CYB5B.
GenevisibleiQ9CQX2. MM.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
3.10.120.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
[Graphical view]
PRINTSiPR00363. CYTOCHROMEB5.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYB5B_MOUSE
AccessioniPrimary (citable) accession number: Q9CQX2
Secondary accession number(s): Q9D1M6, Q9D8R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: June 1, 2001
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.