Reviewed, UniProtKB/Swiss-Prot Q9CQX2 (CYB5B_MOUSE)

Names and origin

Protein names

Recommended name:
    Cytochrome b5 type B
Alternative name(s):
    Cytochrome b5 outer mitochondrial membrane isoform

Gene names

Name:	Cyb5b
Synonyms:	Cyb5m

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases By similarity.
Subunit structure	Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2 By similarity.
Subcellular location	Mitochondrion outer membrane By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from direct assay. Source: MGI mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 11	11	By similarity		PRO_0000006473
	Chain	12 – 146	135	Cytochrome b5 type B		PRO_0000006474
Regions
	Transmembrane	119 – 136	18	Potential
	Domain	20 – 96	77	Cytochrome b5 heme-binding
Sites
	Metal binding	55	1	Iron (heme axial ligand) By similarity
	Metal binding	79	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Modified residue	33	1	Phosphoserine Ref.3 Ref.4
Experimental info
	Sequence conflict	31	1	R → E in BAB25251. Ref.1
	Sequence conflict	142	1	D → G in BAB22721. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9CQX2-1 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 4ADA7C96A5F311DD	FASTA	146	16,318
	10 20 30 40 50 60 MATPEASGSG EKVEGSEPSV TYYRLEEVAK RNSAEETWMV IHGRVYDITR FLSEHPGGEE 70 80 90 100 110 120 VLLEQAGADA TESFEDVGHS PDAREMLKQY YIGDVHPSDL KPKGDDKDPS KNNSCQSSWA 130 140 YWFVPIVGAI LIGFLYRHFW ADSKSS

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Mammary gland, Pancreas and Thymus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and FVB/N. Tissue: Brain and Colon.
[3]	"Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS." Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y. Mol. Cell. Proteomics 6:669-676(2007) [PubMed: 17208939] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33, MASS SPECTROMETRY. Tissue: Liver.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33, MASS SPECTROMETRY. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AK003333 mRNA. Translation: BAB22721.1. AK003815 mRNA. Translation: BAB23012.1. AK007780 mRNA. Translation: BAB25251.1. AK019263 mRNA. Translation: BAB31635.1. AK052810 mRNA. Translation: BAC35156.1. AK088970 mRNA. Translation: BAC40677.1. BC054749 mRNA. Translation: AAH54749.1. BC058812 mRNA. Translation: AAH58812.1. BC062980 mRNA. Translation: AAH62980.1.
IPI	IPI00315794.
RefSeq	NP_079834.2.
UniGene	Mm.20242
3D structure databases
HSSP	HSSP built from PDB template 1B5M based on UniProtKB P04166.
SMR	Q9CQX2. Positions 17-102.
ModBase	Search...
PTM databases
PhosphoSite	Q9CQX2.
Proteomic databases
PRIDE	Q9CQX2.
Genome annotation databases
Ensembl	ENSMUSG00000031924. Mus musculus. [Contig view]
GeneID	66427.
KEGG	mmu:66427.
Organism-specific databases
MGI	MGI:1913677. Cyb5b.
Phylogenomic databases
HOVERGEN	Q9CQX2.
OMA	Q9CQX2. IGDVHPS.
Gene expression databases
ArrayExpress	Q9CQX2.
Bgee	Q9CQX2.
CleanEx	MM_CYB5B.
GermOnline	ENSMUSG00000031924. Mus musculus.
Family and domain databases
InterPro	IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
ProDom	PD000612. Cyt_B5. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	321663.
SOURCE	Search...

Entry information

Entry name

CYB5B_MOUSE

Accession

Primary (citable) accession number: Q9CQX2
Secondary accession number(s): Q9D1M6, Q9D8R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)