Q9CQX2 (CYB5B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b5 type B

Alternative name(s):
Cytochrome b5 outer mitochondrial membrane isoform

Gene names

Name:	Cyb5b
Synonyms:	Cyb5m

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	146 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases By similarity.
Subunit structure	Component of a complex composed of cytochrome b5, NADH-cytochrome b5 reductase (CYB5R3) and MOSC2 By similarity.
Subcellular location	Mitochondrion outer membrane By similarity.
Sequence similarities	Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from direct assay. Source: MGI mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 11	11	By similarity		PRO_0000006473
	Chain	12 – 146	135	Cytochrome b5 type B		PRO_0000006474
Regions
	Transmembrane	119 – 136	18	Helical; Potential
	Domain	20 – 96	77	Cytochrome b5 heme-binding
Sites
	Metal binding	55	1	Iron (heme axial ligand) By similarity
	Metal binding	79	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Modified residue	30	1	N6-acetyllysine By similarity
	Modified residue	33	1	Phosphoserine Ref.3 Ref.4
Experimental info
	Sequence conflict	31	1	R → E in BAB25251. Ref.1
	Sequence conflict	142	1	D → G in BAB22721. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9CQX2 [UniParc]. Last modified June 1, 2001. Version 1. Checksum: 4ADA7C96A5F311DD	FASTA	146	16,318
	10 20 30 40 50 60 MATPEASGSG EKVEGSEPSV TYYRLEEVAK RNSAEETWMV IHGRVYDITR FLSEHPGGEE 70 80 90 100 110 120 VLLEQAGADA TESFEDVGHS PDAREMLKQY YIGDVHPSDL KPKGDDKDPS KNNSCQSSWA 130 140 YWFVPIVGAI LIGFLYRHFW ADSKSS

References

[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Mammary gland, Pancreas and Thymus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6 and FVB/N. Tissue: Brain and Colon.
[3]	"Mitochondrial phosphoproteome revealed by an improved IMAC method and MS/MS/MS." Lee J., Xu Y., Chen Y., Sprung R., Kim S.C., Xie S., Zhao Y. Mol. Cell. Proteomics 6:669-676(2007) [PubMed: 17208939] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33, MASS SPECTROMETRY. Tissue: Liver.
[4]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-33, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK003333 mRNA. Translation: BAB22721.1. AK003815 mRNA. Translation: BAB23012.1. AK007780 mRNA. Translation: BAB25251.1. AK019263 mRNA. Translation: BAB31635.1. AK052810 mRNA. Translation: BAC35156.1. AK088970 mRNA. Translation: BAC40677.1. BC054749 mRNA. Translation: AAH54749.1. BC058812 mRNA. Translation: AAH58812.1. BC062980 mRNA. Translation: AAH62980.1.
IPI	IPI00315794.
RefSeq	NP_079834.2. NM_025558.5.
UniGene	Mm.20242.
3D structure databases
ProteinModelPortal	Q9CQX2.
SMR	Q9CQX2. Positions 18-102.
ModBase	Search...
PTM databases
PhosphoSite	Q9CQX2.
Proteomic databases
PRIDE	Q9CQX2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034400; ENSMUSP00000034400; ENSMUSG00000031924.
GeneID	66427.
KEGG	mmu:66427.
UCSC	uc009nhg.1. mouse.
Organism-specific databases
CTD	80777.
MGI	MGI:1913677. Cyb5b.
Phylogenomic databases
GeneTree	ENSGT00510000046507.
HOGENOM	HBG629516.
HOVERGEN	HBG002653.
InParanoid	Q9CQX2.
OMA	DVHPSDL.
OrthoDB	EOG40ZR00.
PhylomeDB	Q9CQX2.
Gene expression databases
ArrayExpress	Q9CQX2.
Bgee	Q9CQX2.
CleanEx	MM_CYB5B.
Genevestigator	Q9CQX2.
GermOnline	ENSMUSG00000031924. Mus musculus.
Family and domain databases
InterPro	IPR023271. Aquaporin-like. IPR001199. Cyt_B5. IPR018506. Cyt_B5_heme-BS. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit. G3DSA:1.20.1080.10. MIP. 1 hit.
Pfam	PF00173. Cyt-b5. 1 hit. [Graphical view]
PRINTS	PR00363. CYTOCHROMEB5.
SUPFAM	SSF55856. Cyt_B5. 1 hit.
PROSITE	PS00191. CYTOCHROME_B5_1. 1 hit. PS50255. CYTOCHROME_B5_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	321663.
SOURCE	Search...

Entry information

Entry name

CYB5B_MOUSE

Accession

Primary (citable) accession number: Q9CQX2
Secondary accession number(s): Q9D1M6, Q9D8R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	June 1, 2001
Last modified:	September 21, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program