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Protein

Ubiquitin thioesterase OTUB2

Gene

Otub2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei48 – 481By similarity
Active sitei51 – 511NucleophileBy similarity
Active sitei224 – 2241By similarity
Sitei226 – 2261Required to orient and stabilize the active site H-224By similarity

GO - Molecular functioni

  1. omega peptidase activity Source: InterPro
  2. ubiquitin-specific protease activity Source: UniProtKB

GO - Biological processi

  1. cellular amino acid metabolic process Source: Ensembl
  2. protein deubiquitination Source: UniProtKB
  3. protein K11-linked deubiquitination Source: UniProtKB
  4. protein K48-linked deubiquitination Source: UniProtKB
  5. protein K63-linked deubiquitination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC65.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin thioesterase OTUB2 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme OTUB2
OTU domain-containing ubiquitin aldehyde-binding protein 2
Otubain-2
Ubiquitin-specific-processing protease OTUB2
Gene namesi
Name:Otub2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:1915399. Otub2.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 234234Ubiquitin thioesterase OTUB2PRO_0000221011Add
BLAST

Proteomic databases

MaxQBiQ9CQX0.
PaxDbiQ9CQX0.
PRIDEiQ9CQX0.

PTM databases

PhosphoSiteiQ9CQX0.

Expressioni

Gene expression databases

BgeeiQ9CQX0.
CleanExiMM_OTUB2.
ExpressionAtlasiQ9CQX0. baseline and differential.
GenevestigatoriQ9CQX0.

Structurei

3D structure databases

ProteinModelPortaliQ9CQX0.
SMRiQ9CQX0. Positions 2-232.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 231192OTUPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C65 family.Curated
Contains 1 OTU domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG267426.
GeneTreeiENSGT00390000006979.
HOGENOMiHOG000019496.
HOVERGENiHBG053383.
InParanoidiQ9CQX0.
KOiK09603.
OrthoDBiEOG71K64D.
PhylomeDBiQ9CQX0.
TreeFamiTF314145.

Family and domain databases

InterProiIPR003323. OTU.
IPR030299. OTUB2.
IPR016615. Otubain.
IPR019400. Peptidase_C65_otubain.
[Graphical view]
PANTHERiPTHR12931:SF3. PTHR12931:SF3. 1 hit.
PfamiPF10275. Peptidase_C65. 1 hit.
[Graphical view]
PIRSFiPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9CQX0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSETSFNLIS EKCDILSILR DHPENRIYQR KIQELSKRFT SIRKTKGDGN
60 70 80 90 100
CFYRALGYSY LESLLGKSRE ILKFKERVLQ TPNDLLAAGF EEHKFRNFFN
110 120 130 140 150
AFYSVVELVE KDSSVSSLLK VFNDQSSSDR IVQFLRLLTS AFIRNRADFF
160 170 180 190 200
RHFIDEEMDI KDFCTHEVEP MAMECDHVQI TALSQALNIA LQVEYVDEMD
210 220 230
TALNHHVFPE AAIPSVYLLY KTSHYNILYA AEKH
Length:234
Mass (Da):27,300
Last modified:June 1, 2001 - v1
Checksum:i4BE469C91CE4EAD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006054 mRNA. Translation: BAB24385.1.
AK006346 mRNA. Translation: BAB24539.1.
AK006469 mRNA. Translation: BAB24603.1.
AK019830 mRNA. Translation: BAB31872.1.
AK160505 mRNA. Translation: BAE35829.1.
BC049551 mRNA. Translation: AAH49551.1.
CCDSiCCDS36528.1.
RefSeqiNP_001171312.1. NM_001177841.1.
NP_080856.1. NM_026580.4.
UniGeneiMm.127378.

Genome annotation databases

EnsembliENSMUST00000021620; ENSMUSP00000021620; ENSMUSG00000021203.
GeneIDi68149.
KEGGimmu:68149.
UCSCiuc007ovg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006054 mRNA. Translation: BAB24385.1.
AK006346 mRNA. Translation: BAB24539.1.
AK006469 mRNA. Translation: BAB24603.1.
AK019830 mRNA. Translation: BAB31872.1.
AK160505 mRNA. Translation: BAE35829.1.
BC049551 mRNA. Translation: AAH49551.1.
CCDSiCCDS36528.1.
RefSeqiNP_001171312.1. NM_001177841.1.
NP_080856.1. NM_026580.4.
UniGeneiMm.127378.

3D structure databases

ProteinModelPortaliQ9CQX0.
SMRiQ9CQX0. Positions 2-232.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC65.002.

PTM databases

PhosphoSiteiQ9CQX0.

Proteomic databases

MaxQBiQ9CQX0.
PaxDbiQ9CQX0.
PRIDEiQ9CQX0.

Protocols and materials databases

DNASUi68149.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021620; ENSMUSP00000021620; ENSMUSG00000021203.
GeneIDi68149.
KEGGimmu:68149.
UCSCiuc007ovg.2. mouse.

Organism-specific databases

CTDi78990.
MGIiMGI:1915399. Otub2.

Phylogenomic databases

eggNOGiNOG267426.
GeneTreeiENSGT00390000006979.
HOGENOMiHOG000019496.
HOVERGENiHBG053383.
InParanoidiQ9CQX0.
KOiK09603.
OrthoDBiEOG71K64D.
PhylomeDBiQ9CQX0.
TreeFamiTF314145.

Miscellaneous databases

NextBioi326534.
PROiQ9CQX0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9CQX0.
CleanExiMM_OTUB2.
ExpressionAtlasiQ9CQX0. baseline and differential.
GenevestigatoriQ9CQX0.

Family and domain databases

InterProiIPR003323. OTU.
IPR030299. OTUB2.
IPR016615. Otubain.
IPR019400. Peptidase_C65_otubain.
[Graphical view]
PANTHERiPTHR12931:SF3. PTHR12931:SF3. 1 hit.
PfamiPF10275. Peptidase_C65. 1 hit.
[Graphical view]
PIRSFiPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
PROSITEiPS50802. OTU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Testis and Tongue.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiOTUB2_MOUSE
AccessioniPrimary (citable) accession number: Q9CQX0
Secondary accession number(s): Q3TUZ2, Q9D4K8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2001
Last modified: February 4, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.