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Q9CQX0

- OTUB2_MOUSE

UniProt

Q9CQX0 - OTUB2_MOUSE

Protein

Ubiquitin thioesterase OTUB2

Gene

Otub2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Jun 2001)
      Previous versions | rss
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    Functioni

    Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei48 – 481By similarity
    Active sitei51 – 511NucleophileBy similarity
    Active sitei224 – 2241By similarity
    Sitei226 – 2261Required to orient and stabilize the active site H-224By similarity

    GO - Molecular functioni

    1. omega peptidase activity Source: InterPro
    2. ubiquitin-specific protease activity Source: UniProtKB

    GO - Biological processi

    1. cellular amino acid metabolic process Source: UniProtKB
    2. protein deubiquitination Source: UniProtKB
    3. protein K11-linked deubiquitination Source: UniProtKB
    4. protein K48-linked deubiquitination Source: UniProtKB
    5. protein K63-linked deubiquitination Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC65.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin thioesterase OTUB2 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme OTUB2
    OTU domain-containing ubiquitin aldehyde-binding protein 2
    Otubain-2
    Ubiquitin-specific-processing protease OTUB2
    Gene namesi
    Name:Otub2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1915399. Otub2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 234234Ubiquitin thioesterase OTUB2PRO_0000221011Add
    BLAST

    Proteomic databases

    PaxDbiQ9CQX0.
    PRIDEiQ9CQX0.

    PTM databases

    PhosphoSiteiQ9CQX0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9CQX0.
    BgeeiQ9CQX0.
    CleanExiMM_OTUB2.
    GenevestigatoriQ9CQX0.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9CQX0.
    SMRiQ9CQX0. Positions 2-232.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 231192OTUPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C65 family.Curated
    Contains 1 OTU domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG267426.
    GeneTreeiENSGT00390000006979.
    HOGENOMiHOG000019496.
    HOVERGENiHBG053383.
    KOiK09603.
    OrthoDBiEOG71K64D.
    PhylomeDBiQ9CQX0.
    TreeFamiTF314145.

    Family and domain databases

    InterProiIPR003323. OTU.
    IPR019400. Peptidase_C65_otubain.
    IPR016615. Ubiquitin_thioesterase_Otubain.
    [Graphical view]
    PfamiPF10275. Peptidase_C65. 1 hit.
    [Graphical view]
    PIRSFiPIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
    PROSITEiPS50802. OTU. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9CQX0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSETSFNLIS EKCDILSILR DHPENRIYQR KIQELSKRFT SIRKTKGDGN    50
    CFYRALGYSY LESLLGKSRE ILKFKERVLQ TPNDLLAAGF EEHKFRNFFN 100
    AFYSVVELVE KDSSVSSLLK VFNDQSSSDR IVQFLRLLTS AFIRNRADFF 150
    RHFIDEEMDI KDFCTHEVEP MAMECDHVQI TALSQALNIA LQVEYVDEMD 200
    TALNHHVFPE AAIPSVYLLY KTSHYNILYA AEKH 234
    Length:234
    Mass (Da):27,300
    Last modified:June 1, 2001 - v1
    Checksum:i4BE469C91CE4EAD4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK006054 mRNA. Translation: BAB24385.1.
    AK006346 mRNA. Translation: BAB24539.1.
    AK006469 mRNA. Translation: BAB24603.1.
    AK019830 mRNA. Translation: BAB31872.1.
    AK160505 mRNA. Translation: BAE35829.1.
    BC049551 mRNA. Translation: AAH49551.1.
    CCDSiCCDS36528.1.
    RefSeqiNP_001171312.1. NM_001177841.1.
    NP_080856.1. NM_026580.4.
    UniGeneiMm.127378.

    Genome annotation databases

    EnsembliENSMUST00000021620; ENSMUSP00000021620; ENSMUSG00000021203.
    GeneIDi68149.
    KEGGimmu:68149.
    UCSCiuc007ovg.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK006054 mRNA. Translation: BAB24385.1 .
    AK006346 mRNA. Translation: BAB24539.1 .
    AK006469 mRNA. Translation: BAB24603.1 .
    AK019830 mRNA. Translation: BAB31872.1 .
    AK160505 mRNA. Translation: BAE35829.1 .
    BC049551 mRNA. Translation: AAH49551.1 .
    CCDSi CCDS36528.1.
    RefSeqi NP_001171312.1. NM_001177841.1.
    NP_080856.1. NM_026580.4.
    UniGenei Mm.127378.

    3D structure databases

    ProteinModelPortali Q9CQX0.
    SMRi Q9CQX0. Positions 2-232.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C65.002.

    PTM databases

    PhosphoSitei Q9CQX0.

    Proteomic databases

    PaxDbi Q9CQX0.
    PRIDEi Q9CQX0.

    Protocols and materials databases

    DNASUi 68149.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021620 ; ENSMUSP00000021620 ; ENSMUSG00000021203 .
    GeneIDi 68149.
    KEGGi mmu:68149.
    UCSCi uc007ovg.2. mouse.

    Organism-specific databases

    CTDi 78990.
    MGIi MGI:1915399. Otub2.

    Phylogenomic databases

    eggNOGi NOG267426.
    GeneTreei ENSGT00390000006979.
    HOGENOMi HOG000019496.
    HOVERGENi HBG053383.
    KOi K09603.
    OrthoDBi EOG71K64D.
    PhylomeDBi Q9CQX0.
    TreeFami TF314145.

    Miscellaneous databases

    NextBioi 326534.
    PROi Q9CQX0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9CQX0.
    Bgeei Q9CQX0.
    CleanExi MM_OTUB2.
    Genevestigatori Q9CQX0.

    Family and domain databases

    InterProi IPR003323. OTU.
    IPR019400. Peptidase_C65_otubain.
    IPR016615. Ubiquitin_thioesterase_Otubain.
    [Graphical view ]
    Pfami PF10275. Peptidase_C65. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
    PROSITEi PS50802. OTU. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Testis and Tongue.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiOTUB2_MOUSE
    AccessioniPrimary (citable) accession number: Q9CQX0
    Secondary accession number(s): Q3TUZ2, Q9D4K8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2004
    Last sequence update: June 1, 2001
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3