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Protein

14-3-3 protein beta/alpha

Gene

Ywhab

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-165159. mTOR signalling.
R-MMU-166208. mTORC1-mediated signalling.
R-MMU-2028269. Signaling by Hippo.
R-MMU-392517. Rap1 signalling.
R-MMU-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.
R-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-5673000. RAF activation.
R-MMU-5674135. MAP2K and MAPK activation.
R-MMU-5675221. Negative regulation of MAPK pathway.
R-MMU-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein beta/alpha
Alternative name(s):
Protein kinase C inhibitor protein 1
Short name:
KCIP-1
Cleaved into the following chain:
Gene namesi
Name:Ywhab
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1891917. Ywhab.

Subcellular locationi

  • Cytoplasm By similarity
  • Melanosome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003679021 – 24614-3-3 protein beta/alphaAdd BLAST246
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00000000052 – 24614-3-3 protein beta/alpha, N-terminally processedAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in 14-3-3 protein beta/alpha, N-terminally processedBy similarity1
Modified residuei2PhosphothreonineBy similarity1
Modified residuei60Phosphoserine1 Publication1
Modified residuei70N6-acetyllysineBy similarity1
Modified residuei84Nitrated tyrosineCombined sources1
Modified residuei106Nitrated tyrosineCombined sources1
Modified residuei117N6-acetyllysineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Modified residuei232PhosphoserineBy similarity1
Isoform Short (identifier: Q9CQV8-2)
Modified residuei1N-acetylmethionine By similarityBy similarity1

Post-translational modificationi

Isoform alpha differs from isoform beta in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.By similarity1 Publication
Isoform Short contains a N-acetylmethionine at position 1.By similarity

Keywords - PTMi

Acetylation, Nitration, Phosphoprotein

Proteomic databases

PaxDbiQ9CQV8.
PeptideAtlasiQ9CQV8.
PRIDEiQ9CQV8.
TopDownProteomicsiQ9CQV8-1. [Q9CQV8-1]

2D gel databases

UCD-2DPAGEQ9CQV8.

PTM databases

iPTMnetiQ9CQV8.
PhosphoSitePlusiQ9CQV8.
SwissPalmiQ9CQV8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000018326.
ExpressionAtlasiQ9CQV8. baseline and differential.
GenevisibleiQ9CQV8. MM.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with SAMSN1 and PRKCE (PubMed:18604201, PubMed:20478393). Interacts with AKAP13. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2. Interacts with YAP1 (phosphorylated form). Interacts with the phosphorylated (by AKT1) form of SRPK2. Interacts with PKA-phosphorylated AANAT. Interacts with MYO1C. Interacts with SIRT2 (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (By similarity). Interacts (via phosphorylated form) with YWHAB; this interaction occurs in a protein kinase AKT1-dependent manner (By similarity).By similarity3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei58Interaction with phosphoserine on interacting proteinBy similarity1
Sitei129Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
Lrrk2Q5S0063EBI-771608,EBI-2693710
PrkceP160546EBI-771608,EBI-298451
Synpo2Q91YE83EBI-771608,EBI-7623057

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207648. 23 interactors.
IntActiQ9CQV8. 626 interactors.
MINTiMINT-1869492.
STRINGi10090.ENSMUSP00000018470.

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 17Combined sources13
Helixi21 – 32Combined sources12
Helixi40 – 68Combined sources29
Helixi77 – 105Combined sources29
Helixi107 – 110Combined sources4
Helixi114 – 134Combined sources21
Helixi137 – 161Combined sources25
Helixi167 – 182Combined sources16
Helixi187 – 203Combined sources17
Helixi205 – 207Combined sources3
Turni210 – 212Combined sources3
Helixi213 – 230Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GNTX-ray2.41A1-239[»]
5F74X-ray2.35A1-246[»]
ProteinModelPortaliQ9CQV8.
SMRiQ9CQV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiQ9CQV8.
KOiK16197.
OMAiMGREYRE.
OrthoDBiEOG091G0VKY.
PhylomeDBiQ9CQV8.
TreeFamiTF102003.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Long (identifier: Q9CQV8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY
60 70 80 90 100
KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL
110 120 130 140 150
ELLDKYLILN ATQAESKVFY LKMKGDYFRY LSEVASGENK QTTVSNSQQA
160 170 180 190 200
YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE
210 220 230 240
AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN
Length:246
Mass (Da):28,086
Last modified:January 23, 2007 - v3
Checksum:i51C366ED85B38EED
GO
Isoform Short (identifier: Q9CQV8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: Missing.

Note: No experimental confirmation available.By similarity
Show »
Length:244
Mass (Da):27,854
Checksum:iF42896ED9EC6F0D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10Q → H in AAC14343 (Ref. 1) Curated1
Sequence conflicti74N → D in AAC14343 (Ref. 1) Curated1
Sequence conflicti126D → Y in BAE29538 (PubMed:16141072).Curated1
Sequence conflicti126D → Y in BAE30278 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186341 – 2Missing in isoform Short. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058797 mRNA. Translation: AAC14343.1.
AK002632 mRNA. Translation: BAB22246.1.
AK004872 mRNA. Translation: BAB23631.1.
AK011389 mRNA. Translation: BAB27587.1.
AK083367 mRNA. Translation: BAC38886.1.
AK144061 mRNA. Translation: BAE25678.1.
AK150414 mRNA. Translation: BAE29538.1.
AK151294 mRNA. Translation: BAE30278.1.
AK158932 mRNA. Translation: BAE34730.1.
CCDSiCCDS17019.1. [Q9CQV8-1]
RefSeqiNP_061223.2. NM_018753.6. [Q9CQV8-1]
XP_006499972.1. XM_006499909.1. [Q9CQV8-1]
UniGeneiMm.34319.
Mm.491117.

Genome annotation databases

EnsembliENSMUST00000018470; ENSMUSP00000018470; ENSMUSG00000018326. [Q9CQV8-1]
GeneIDi54401.
KEGGimmu:54401.
UCSCiuc008ntp.1. mouse. [Q9CQV8-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058797 mRNA. Translation: AAC14343.1.
AK002632 mRNA. Translation: BAB22246.1.
AK004872 mRNA. Translation: BAB23631.1.
AK011389 mRNA. Translation: BAB27587.1.
AK083367 mRNA. Translation: BAC38886.1.
AK144061 mRNA. Translation: BAE25678.1.
AK150414 mRNA. Translation: BAE29538.1.
AK151294 mRNA. Translation: BAE30278.1.
AK158932 mRNA. Translation: BAE34730.1.
CCDSiCCDS17019.1. [Q9CQV8-1]
RefSeqiNP_061223.2. NM_018753.6. [Q9CQV8-1]
XP_006499972.1. XM_006499909.1. [Q9CQV8-1]
UniGeneiMm.34319.
Mm.491117.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4GNTX-ray2.41A1-239[»]
5F74X-ray2.35A1-246[»]
ProteinModelPortaliQ9CQV8.
SMRiQ9CQV8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207648. 23 interactors.
IntActiQ9CQV8. 626 interactors.
MINTiMINT-1869492.
STRINGi10090.ENSMUSP00000018470.

PTM databases

iPTMnetiQ9CQV8.
PhosphoSitePlusiQ9CQV8.
SwissPalmiQ9CQV8.

2D gel databases

UCD-2DPAGEQ9CQV8.

Proteomic databases

PaxDbiQ9CQV8.
PeptideAtlasiQ9CQV8.
PRIDEiQ9CQV8.
TopDownProteomicsiQ9CQV8-1. [Q9CQV8-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018470; ENSMUSP00000018470; ENSMUSG00000018326. [Q9CQV8-1]
GeneIDi54401.
KEGGimmu:54401.
UCSCiuc008ntp.1. mouse. [Q9CQV8-1]

Organism-specific databases

CTDi7529.
MGIiMGI:1891917. Ywhab.

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiQ9CQV8.
KOiK16197.
OMAiMGREYRE.
OrthoDBiEOG091G0VKY.
PhylomeDBiQ9CQV8.
TreeFamiTF102003.

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-165159. mTOR signalling.
R-MMU-166208. mTORC1-mediated signalling.
R-MMU-2028269. Signaling by Hippo.
R-MMU-392517. Rap1 signalling.
R-MMU-450385. Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA.
R-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-5673000. RAF activation.
R-MMU-5674135. MAP2K and MAPK activation.
R-MMU-5675221. Negative regulation of MAPK pathway.
R-MMU-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Miscellaneous databases

ChiTaRSiYwhab. mouse.
PROiQ9CQV8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018326.
ExpressionAtlasiQ9CQV8. baseline and differential.
GenevisibleiQ9CQV8. MM.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei1433B_MOUSE
AccessioniPrimary (citable) accession number: Q9CQV8
Secondary accession number(s): O70455, Q3TY33, Q3UAN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 154 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.