Q9CQV7 (TIM14_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitochondrial import inner membrane translocase subunit TIM14

Alternative name(s):
DnaJ homolog subfamily C member 19

Gene names

Name:	Dnajc19
Synonyms:	Tim14, Timm14

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. May act as a co-chaperone that stimulate the ATP-dependent activity By similarity.
Subunit structure	Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19 By similarity.
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the TIM14 family. Contains 1 J domain.

Ontologies

Keywords
Biological process	Protein transport Translocation Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	Chaperone
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9CQV7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9CQV7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     95-116: GSPYIAAKINEAKDLLEGQAKK → PLVEEGLKPI...TPVKPCETTM

Note: No experimental confirmation available.

Isoform 3 (identifier: Q9CQV7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     95-116: GSPYIAAKINEAKDLLEGQAKK → KQLLLLYWTVNGIANSWCYECLCVSSFPVVDHIVVS

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 116	115	Mitochondrial import inner membrane translocase subunit TIM14		PRO_0000071101
Regions
	Topological domain	2 – 3	2	Mitochondrial intermembrane Potential
	Transmembrane	4 – 24	21	Helical; Potential
	Topological domain	25 – 116	92	Mitochondrial matrix Potential
	Domain	62 – 116	55	J
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	70	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	95 – 116	22	GSPYI…GQAKK → PLVEEGLKPIPICRSCSSIC RRSILSCSTSYDQNKNPFTV CVCVHSSAHTGIHTPVKPCE TTM in isoform 2.		VSP_016390
	Alternative sequence	95 – 116	22	GSPYI…GQAKK → KQLLLLYWTVNGIANSWCYE CLCVSSFPVVDHIVVS in isoform 3.		VSP_016391

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 51E4313DB4F6083E	FASTA	116	12,437
	10 20 30 40 50 60 MASTVVAVGL TIAAAGFAGR YVLQAMKHVE PQVKQVFQSL PKSAFGGGYY RGGFEPKMTK 70 80 90 100 110 REAALILGVS PTANKGKIRD AHRRIMLLNH PDKGGSPYIA AKINEAKDLL EGQAKK
	Isoform 2 [UniParc]. Checksum: 7D8BC9DF54F2BDAA	FASTA	157	16,956
	10 20 30 40 50 60 MASTVVAVGL TIAAAGFAGR YVLQAMKHVE PQVKQVFQSL PKSAFGGGYY RGGFEPKMTK 70 80 90 100 110 120 REAALILGVS PTANKGKIRD AHRRIMLLNH PDKGPLVEEG LKPIPICRSC SSICRRSILS 130 140 150 CSTSYDQNKN PFTVCVCVHS SAHTGIHTPV KPCETTM
	Isoform 3 [UniParc]. Checksum: A7EE66196A6A7B2F	FASTA	130	14,193
	10 20 30 40 50 60 MASTVVAVGL TIAAAGFAGR YVLQAMKHVE PQVKQVFQSL PKSAFGGGYY RGGFEPKMTK 70 80 90 100 110 120 REAALILGVS PTANKGKIRD AHRRIMLLNH PDKGKQLLLL YWTVNGIANS WCYECLCVSS 130 FPVVDHIVVS

References

[1]

"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Pancreas, Small intestine and Tongue.

[2]

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: FVB/N. Tissue: Kidney.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK007866 mRNA. Translation: BAB25317.1. AK008267 mRNA. Translation: BAB25565.1. AK010204 mRNA. Translation: BAB26767.1. AK012051 mRNA. Translation: BAB27993.1. BC024335 mRNA. Translation: AAH24335.1.
IPI	IPI00111111. IPI00468992. IPI00624902.
RefSeq	NP_001021382.1. NM_001026211.1. NP_080608.3. NM_026332.3. XP_003084767.1. XM_003084719.1.
UniGene	Mm.274266. Mm.389927.
3D structure databases
ProteinModelPortal	Q9CQV7.
SMR	Q9CQV7. Positions 48-111.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000011029.
PTM databases
PhosphoSite	Q9CQV7.
Proteomic databases
PaxDb	Q9CQV7.
PRIDE	Q9CQV7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000108195; ENSMUSP00000103830; ENSMUSG00000027679. ENSMUST00000117223; ENSMUSP00000113484; ENSMUSG00000027679.
GeneID	100503724. 67713.
KEGG	mmu:100503724. mmu:67713.
UCSC	uc008oxm.1. mouse. uc008oxo.1. mouse. uc008oxp.1. mouse.
Organism-specific databases
CTD	131118.
MGI	MGI:1914963. Dnajc19.
Phylogenomic databases
eggNOG	NOG250674.
GeneTree	ENSGT00390000017303.
HOGENOM	HOG000115841.
HOVERGEN	HBG057483.
KO	K09539.
OMA	NKIREAH.
OrthoDB	EOG43XV50.
Gene expression databases
ArrayExpress	Q9CQV7.
Bgee	Q9CQV7.
CleanEx	MM_DNAJC19.
Genevestigator	Q9CQV7.
GermOnline	ENSMUSG00000057330. Mus musculus.
Family and domain databases
Gene3D	1.10.287.110. 1 hit.
InterPro	IPR001623. DnaJ_domain. [Graphical view]
Pfam	PF00226. DnaJ. 1 hit. [Graphical view]
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
SUPFAM	SSF46565. DnaJ_N. 1 hit.
PROSITE	PS00636. DNAJ_1. False negative. PS50076. DNAJ_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	325345.
SOURCE	Search...

Entry information

Entry name

TIM14_MOUSE

Accession

Primary (citable) accession number: Q9CQV7
Secondary accession number(s): Q8R1N1, Q9D896

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program